The invention provides nucleic acid preserving fluid. The nucleic acid preserving fluid consists of a nuclease inhibitor, a protein denaturant, a stabilizer, a bacteriostatic agent and a Tricine buffer solution, wherein the concentration of the nuclease inhibitor is 5mg / ml-20mg / ml, the concentration of the protein denaturant is 20mg / ml-80mg / ml, the concentration of the stabilizer is 30mg / ml-90mg / ml, the concentration of the bacteriostatic agent is 100mg / ml-500mg / ml, and the concentration of the Tricine buffer solution is 20nM-120nM. The nucleic acid preserving fluid has the beneficial effectsthat the nucleic acid preserving fluid is capable of effectively preventing degradation of nucleic acid at a normal temperature and providing a stable environment for nucleic acid, so that the preservation time of the nucleic acid is prolonged, and the safety during collection, transportation and detection can be guaranteed; and meanwhile, pathogenic microorganisms can be inactivated, and the nucleic acid preserving fluid has the advantages of low preparation cost, stable performance, easiness in preservation and the like.