1286 results about "Electrophoresis band" patented technology

Methods using gel electrophoresis and mass spectrometry for the rapid, quantitative analysis of proteins or protein function in mixtures of proteins derived from two or more samples in one unit operation are disclosed. In one embodiment the method includes (a) preparing an extract of proteins from each of at least two different samples; (b) providing a set of substantially chemically identical and differentially isotopically labeled protein reagents; (c) reacting the extract of proteins from different samples of step (a) with a different isotopically labeled reagent from the set of step (b) to provide two or more sets of isotopically differentially labeled proteins; (d) mixing each of the two or more sets of isotopically labeled proteins to form a single mixture of isotopically differentially labeled proteins; (e) electrophoresing the mixture of step (d) by an electrophoresing method capable of separating proteins within the mixture; (f) digesting at least a portion of one or more separated proteins of step (e) and (g) detecting the difference in the expression levels of the proteins in the two samples by mass spectrometry based on one or more peptides in the sample of labeled peptides. The analytical method can be used for qualitative and particularly for quantitative analysis of global protein expression profiles in cells and tissues, i.e. the quantitative analysis of proteomes.

A gel electrophoresis training aid is provided. The training aid includes a substantially transparent elastomeric body having at least one series of spaced-apart wells. The elastomeric body is adapted with a plurality of spaced-apart bands extending in the same direction from the wells. Each of the spaced-apart bands are positioned substantially parallel to one spaced-apart well. A training kit including the training aid is also provided.

The invention provides a method for extracting keratin from feather, comprising the steps that after being crashed, clean feather is pretreated by ethanol and hydrochloric acid; by adopting mercaptoethanol reduction method, the pretreated feather is deacidized to obtain keratin solution of the feather, and then clean sand white keratin solid powder is obtained through acid coprecipitate, washing and drying. The extracted keratin is analyzed and represented by the methods such as ultraviolet-visible spectrum, infrared spectrum, SDS-gel electrophoresis, elementary analysis, etc. The results shows that the keratin extracted by the method mainly contains the elements such as C, H, N, O, S, the total content of which reaches over 99%, and the purity is higher; the molecular weight of the keratin mainly ranges from 5 to 20 kD and 40 to 80kD.

Various traveling wave grids and electrophoretic systems, and electrode assemblies using such grids, are disclosed. A configuration in which a voltage potential is used to load a biomolecule sample against a grid is disclosed. A unique strategy of using multiple, reconfigurable grids in such systems is also described. The strategy involves initially conducting a broad protein separation and then selectively tailoring one or more grids, and conducting one or more secondary processing operations. Related strategies and specific methods are additionally disclosed for separating samples of biomolecules and components thereof using the noted systems, assemblies, and grids.

The invention relates to 12 identified protein biomarkers for diagnosis, determination of disease severity, and therapeutic response monitoring of patients with breast cancer. The method is based on the use of 2-dimensional (2D) gel electrophoresis to separate the complex mixture of proteins found in blood serum, the quantitation of up to 12 protein biomarkers, and statistical analysis of the concentration of the protein biomarkers.

Electrophoresis systems, assemblies, cassettes and methods for easily, and more effectively and efficiently, isolating a biomolecule band from an electrophoretic gel are provided. The methods use an electrophoresis cassette with at least one loading well and at lest one collection well. A sample containing the biomolecule of interest is placed into at least one loading well and buffer or water is placed in at lest one collection well. An electric field is then applied to drive migration and separation of the sample into different component bands within the gel. When the component of interest is located within at least one collection well, the electric field is terminated and the buffer or water in the collection well is removed, thereby isolating and collecting the sample component of interest.