The invention relates to a culture-free biochemical method for analyzing the microbial
community structure of
yeast for traditional brewage, in particular to a method for analyzing the microbial
community structure of
yeast for traditional brewage by using the
phospholipid fatty acid spectrogram analysis technique. The method comprises the following steps: (1) weighting a certain amount of
yeast sample which is pulverized and evenly mixed; (2) adding a
buffer solution and an organic
reagent to obtain an organic phase containing lipids; (3) separating the organic phase by small
silica gel columns, eluting and collecting organic components containing
polar lipids; (4) carrying out methyl esterification under mild condition, and adding an extracting
reagent to obtain corresponding
fatty acid methyl ester; (5) carrying out quantitative analysis on the
fatty acid methyl ester by using a gas chromatogram-
mass spectrogram combined instrument; and (6) determining the characteristics of the microbial
community structure in the sample according to characteristics of different
phospholipid fatty acid compositions. The invention provides an accurate and quick culture-free detection means for researching the composition of the microbial
community structure of yeast for brewage and knowing the law of growth and decline of different microbial communities in the yeast preparation process.