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530 results about "Microbial oil" patented technology

Microbial oil is a triglyceride (lipid) produced by a microbe, and is similar to vegetable oil, another biologically produced oil. Some species of yeast are able to convert food into triglycerides and accumulate the produced lipids when fed carbohydrates. Production of microbial oil has been researched for production of biodiesel, because impure carbohydrates such as agricultural residues, e.g. waste molasses can be used as a feedstock for production of oil.

Mortierella alpina C16/18 fatty acid elongase

The present invention relates to a fungal C16 / 18 fatty acid elongase that is able to catalyze the conversion of palmitate (16:0) to stearic acid (18:0). Specifically, the nucleotide sequence of a Mortierella alpina C16 / 18 fatty acid elongase is provided (designated as “ELO3”). Methods of increasing microbial oil production, increasing carbon flux into the polyunsaturated fatty acid biosynthetic pathway and increasing the content of polyunsaturated fatty acids by over-expression of the C16 / 18 fatty acid elongase are described herein. Most desirably, the substrate specificity of the instant ELO3 will be particularly useful to enable accumulation of long-chain polyunsaturated fatty acids in oleaginous yeast, such as Yarrowia lipolytica.
Owner:DUPONT US HLDG LLC

Separation and extraction method of microbial oil

A method for separating and extracting microbial oil comprises the following steps: (1) microbial strains are inoculated for fermentation; (2) the obtained fermentation liquor is concentrated to remove 45 percent to 55 percent of water in the fermentation liquor so as to obtain the concentrated fermentaton liquor; (3) the concentrated fermentation liquor is fed into a high pressure homogenizer with the pressure of 70 to 130MPa to carry out cell-crushing; (4) an extractive solvent is added, and after two times of extraction, the upper layer organic solution phase is separated and collected to obtain mixed oil; and (5) at last, the solvent in the mixed oil is vaporized and recycled to obtain the microbial oil. The method combines the fermented solution concentration with the high pressure homogenization technology to carry out cell crushing, and then adopts the two-time extraction to collect the contained oil and obtain bacterial protein accordingly. The process improves the original method which needs the steps, such as the separation of strains, drying, grinding and granulation, etc., thus greatly simplifying the technology, improving efficiency and reducing production cost. The method homogenizes the fermented solution under high pressure after concentration, which promotes the utilization rate of facilities, and reduces energy consumption.
Owner:ENERGY RES INST OF SHANDONG ACAD OF SCI

Preparation method of human milk fat substitute

ActiveCN102845537AIncrease contentReduced total palmitic acid contentEdible oils/fatsFood preparationMicrobial oilButterfat
The invention relates to a preparation method of a human milk fat substitute. The method comprises the steps of: step 1) conducting an acid hydrolysis reaction on a raw material of high melting palm stearin subjected to chemical interesterification and alcohol leaching, and an acyl donor of rapeseed oil fatty acid by using Lipozyme RM IM as a catalyst, so as to reduce the total palmitic acid content and increase the relative content of site-two palmitic acid of the raw material; and step 2) deacidifying an intermediate product; and at the same time, calculating the amount of sunflower seed oil, rapeseed oil, palm kernel oil, algae oil and microbial oil added to the intermediate product according to the composition and distribution of fatty acids in human milk fat, by a grease adding model, so as to ensure composition and distribution of fatty acids in a final product meeting requirements of a human milk fat substitute, as well as realize a maximum addition amount. The invention adopts two steps to produce the human milk fat substitute, in order to reduce usage amount of free fatty acids in the acid hydrolysis reaction; meanwhile, two times of oil additions increase yield. Therefore, the invention greatly reduces the cost, and achieves a more than doubled yield compared with a similar products with a similar cost.
Owner:JIANGNAN UNIV

Preparation method of human milk substitute fat based on triglyceride composition and evaluating method of human milk substitute fat

The invention discloses a preparation method of a human milk substitute fat based on a triglyceride composition and an evaluating method of the human milk substitute fat. The preparation method comprises the following steps: with lard oil as a base raw material, adding sunflower seed oil, canola oil, palm kernel oil, palm oil, microbial oil and algae oil in the lard oil according to a certain proportion in accordance with composition and distribution of fatty acid of human milk fat to obtain mixed oil similar to the human milk fat on the composition and distribution of the fatty acid; and by adopting specific lipase at 1 and 3 sites as catalysts, and similarities between of mixed oil and human milk fat in aspects of composition and distribution of the fatty acid, composition of polyunsaturated fatty acids and triglyceride as indexes, adjusting the composition of the triglyceride of the mixed fat by using a manner of lactone exchanging to obtain a product with relatively high similarity to the human milk fat on the composition of the triglyceride. The human milk substitute fat is obtained by adopting physical mixing and enzyme catalysis ester exchange manners, the production cost can be greatly reduced, and the relatively high similarity is achieved for the product and the human milk fat.
Owner:无锡益语科技发展有限公司

Method for extracting microbial oil

The invention provides a method for extracting microbial oil, which comprises the following steps: (1) allowing an oil-producing microbial strain to ferment and obtaining fermentation liquor; (2) regulating the pH value of the fermentation liquor obtained by the step (1) to 3.5 to 4.5, and allowing microbes to autolyze; (3) extracting by adding an extracting solvent into the fermentation liquor obtained by the step (2), and collecting upper mixed oil; (4) extracting again by adding the extracting solvent into the lower solution obtained by the extraction and separation in the step (3), and collecting upper mixed oil; and (5) mixing the upper mixed oil obtained by the step (3) and the upper mixed oil obtained by the step (4), distilling, recovering the extracting solvent and obtaining the microbial oil product. The method can extract the oil in the cells of microbes effectively, has high oil extraction rate and high oil extraction efficiency, makes process simple and is favorable for large-scale production.
Owner:CABIO BIOTECH WUHAN CO LTD

Method for strengthening indigenous microbes and improving oil recovery by improving microbial florae in oil deposit

The invention provides a method for strengthening indigenous microbes and improving oil recovery by improving microbial florae in an oil deposit. The method comprises the following steps of: (1) analyzing composition of microbial florae in the oil deposit; and (2) introducing exogenous microbes into the oil deposit according to an analysis result, so that the oil deposit with the introduced exogenous microbes contains the following microbes: hydrocarbon-oxiding bacteria, fermentative bacteria, heterotrophic bacteria, methanogenic bacteria, sulphate-reducing bacteria, and nitrate-reducing bacteria. Due to the adoption of the method provided by the invention, by adding the exogenous microbes into the oil deposit, the structure of the microbial florae in the oil deposit is improved, the function of the microbial florae in the oil deposit is strengthened, as a result, the oil displacement effect is improved, and the application range of microbial oil recovery technology is enlarged.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI

Method for detecting oil content of microalgae

The invention relates to lipid producing microalgae, in particular relates to a method for detecting the oil content of microalgae, comprising the following steps of: 1) taking microalgae-cultivated algae liquid, and computing the quantity concentration C of microalgae cells; 2) observing the lipid of the microalgae: adopting a BODIPY (boron-dipyrromethene) 505 / 515 staining fluorescent recording method; staining for 1-2min by adopting dye use liquid with concentration of 1-2mM, and observing and recording accumulation change conditions of single-celled lipid in a photographing way by using excitation light with wave length of 488nm under a fluorescence microscope; recording the number (n) of single-celled oil drop grains according to a photograph, detecting the diameter (d) of each oil drop, and computing the volume of each oil drop according to a ball volume formula, wherein the content of each cell lipid is the sum of the volume of all the oil drops; and computing the lipid content of the algae liquid in unit volume according to the quantity concentration C of the microalgae and the lipid content of a single cell: Vt=CV, wherein C is the concentration of tetraselmis cells, V is the lipid volume of the single cell, and Vt is the lipid content in the unit volume.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Method for improving oil recovery efficiency through microbial oil displacement

The invention discloses a method for improving oil recovery efficiency through microbial oil replacement and belongs to the technical field of microbial oil recovery. The method specifically comprises the following steps of performing in-situ sampling on a test site and detecting indigenous microorganisms, wherein the detected indigenous microorganisms are composed of aerobic, facultative and anaerobic microorganisms; performing oil displacement on oil reservoir through the aerobic microorganisms, filling the aerobic microorganisms or the activator of the aerobic microorganisms and air into the oil reservoir, and detecting the aerobic microorganisms inside the output liquid of the oil reservoir; performing oil displacement on oil reservoir through the facultative microorganisms, filling the facultative microorganisms or the activator of the facultative microorganisms and air into the oil reservoir, and detecting the facultative microorganisms inside the output liquid of the oil reservoir; performing oil displacement on oil reservoir through the anaerobic microorganisms and filling the anaerobic microorganisms or the activator of the anaerobic microorganisms and air into the oil reservoir. According to the method for improving the oil recovery efficiency through the microbial oil replacement, the activators are abundant in sources, low in price and free from damage to stratums. The method for improving the oil recovery efficiency through the microbial oil replacement is simple in process, high in pertinence and operability and good in in-situ testing effects and improves the recovery efficiency of in-situ tests by larger than 10.0%, thereby being capable of being widely applied to in-situ tests for improving the recovery efficiency through microorganisms.
Owner:CHINA PETROLEUM & CHEM CORP +1

Microbe oil production method after polymer drive

The invention discloses a microbial oil production method, including a process of displacement of reservoir oil by using bacterial liquid after polymer flooding of oil layers; the bacterial liquid is a mixed liquid or zymotic fluid of bacillus fusiformis 6# CGMCC No.2439, Potsdam Bacillus brevis Po CGMCC No.2441 or Bacillus licheniformis U1-3 CGMCC No.2437 singly mixed with a culture medium, or compatible liquid of the three with the culture medium. The experimental results of model displacement of reservoir oil show that the bacterial liquid is used for displacement of reservoir oil after the polymer flooding, so that recovery ratio is increased by 3-5 percent (OOIP), the bacterial liquid, coupled with polymer protection slug flooding, is used for displacement of reservoir oil after the polymer flooding, so that the recovery ratio is increased by about 7-9 percent (OOIP), and a method with the bacterial liquid displacement and chemical displacement combined is used for displacement of reservoir oil after the polymer flooding, so that the recovery ratio can be increased by 13% (OOIP). The experimental results show that the method of the invention can be widely applied to the field of oil production engineering, especially the field of microbial intensified oil production, and is suitable for large-scale popularization and application.
Owner:DAQING OILFIELD CO LTD +1
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