Preparation method of human milk fat substitute
A human milk fat substitute and oil technology, applied in food preparation, edible oil/fat, food science, etc., can solve the problems of large amount of fatty acid, high price of tripalmitin, and difficulty in downstream deacidification of increased cost
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Embodiment 1
[0026] Take by weighing 20g high-melting-point palm stearin in the round-bottomed flask, and mix with the free fatty acid that comes from rapeseed oil with the ratio of 1: 10 in molar ratio, add the Lipozyme RM IM of 10wt% in the mixed system (in total substrate weight), and reacted for 4 hours in water at 60° C. and a stirring rate of 250 rpm. After the obtained reaction substrate was centrifuged at 4000rpm to remove the enzyme, the fatty acid was removed by molecular distillation. The molecular distillation conditions were: evaporation temperature, 185°C; heat exchanger temperature, 60°C; rotational speed, 120rpm; absolute pressure, 2Pa. The composition and distribution of fatty acids are shown in the table.
[0027] Fatty acid composition and distribution of table 2 intermediate product
[0028] fatty acid
total
sn-2
%sn-2 a
sn-1,3 b
C12:0
0.3
0.1
11.1
0.4
C14:0
0.4
0.1
8.3
0.6 ...
Embodiment 2
[0058] Take by weighing 20g high-melting-point palm stearin in the round-bottomed flask, and mix with the free fatty acid that comes from rapeseed oil with the ratio of 1: 12 in molar ratio, add the Lipozyme RM IM of 8wt% in the mixing system (in total substrate weight), and reacted for 3 hours in water at 65° C. and a stirring rate of 250 rpm. After the obtained reaction substrate was centrifuged at 4000rpm to remove the enzyme, the fatty acid was removed by molecular distillation. The molecular distillation conditions were: evaporation temperature, 185°C; heat exchanger temperature, 60°C; rotational speed, 120rpm; absolute pressure, 2Pa. The composition and distribution of fatty acids are shown in the table below.
[0059] Fatty acid composition and distribution of table 4 intermediate product
[0060] fatty acid
total
sn-2
%sn-2
sn-1,3
C12:0
0.3
0.1
11.1
0.4
C14:0
0.4
0.1
8.3
0.6
...
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