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Microbial oil extraction method

A technology of microbial oil and extraction method, applied in the field of microbial oil extraction, can solve the problems of large damage to human health and the environment, poor operation safety, etc., and achieve the effects of environmental friendliness, low toxicity and simple treatment process.

Active Publication Date: 2012-07-04
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are less safe to operate and cause great damage to human health and the environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021]According to the method described in literature (Li YH, Zhao ZB, Bai FW. Enzyme Microbial Technology, 2007, 41, 312), cultivate oleaginous yeast R. toruloides AS 2.1389 (the strain comes from the China Common Microorganism Culture Collection Management Center), the fermentation mash with a fermentation density of 114 g dry cells (CDW) / L was obtained, and the oil content of the dry cells was 63%. Take 50 ml of the above fermented mash and microwave it under normal pressure (800 W, 60 s). Add β-1,3-mannanase to a final concentration of 0.28 g / L, treat at pH 5.0, 25 oC for 0.5 h; then add 50 ml of ethyl acetate, mix well at 25 oC, and extract for 1 h , centrifuged to take out the organic phase, and evaporated the solvent to obtain microbial oil, with an extraction rate of 95%.

[0022] Comparing the experimental results of Example 1 and Comparative Example 1, it was shown that after the fermented mash was treated with microwave and β-1,3-mannanase, the oil could be extra...

Embodiment 2

[0024] Other operating conditions are the same as in Example 1, but the step of treating the fermented mash with β-1,3-mannanase is omitted during the extraction process. The extraction rate is 35%. Comparing Example 1 and Example 2, it shows that treatment with β-1,3-mannanase significantly improves the oil extraction rate.

Embodiment 3

[0026] Other operating conditions are the same as in Example 1, but the step of microwave treatment of fermented mash is omitted in the extraction process. The extraction rate is 30%.

[0027] Comparing Example 1 and Example 3, it is shown that microwave treatment before enzyme treatment can significantly increase the oil extraction rate. The reason may be that microwave treatment leads to the inactivation or significant reduction of endogenous protein activity of oleaginous microorganisms, which is more conducive to β -1,3-mannanase to function.

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PUM

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Abstract

The invention discloses a microbial oil extraction method, and belongs to the field of bioengineering downstream technology. Oil-producing microorganism fermented liquor is used as a raw material, and is subjected to enzymolysis and extraction by organic solvent to obtain the microbial oil, wherein the extraction rate is up to 100% maximally. The microbial oil extraction method disclosed by the invention does not need concentration of the fermented liquor, recovery of strains, drying of the strains and other pretreatment operations, has mild enzymolysis conditions, short treatment time, low extraction temperature, high extraction rate, low requirements on equipment and low toxicity of the used organic solvent, greatly reduces the power and energy consumptions in the microbial oil extraction process, lowers the process cost and alleviates the environmental pollution and damage to human health. According to the method, a new way is provided for mass production and extraction of the microbial oil.

Description

technical field [0001] The invention relates to a method for extracting microbial oil. Specifically, the fermentation mash of oil-producing microorganisms is pretreated with enzymes, and then extracted with an organic solvent to obtain oil, which belongs to the downstream technical field of bioengineering. Background technique [0002] Many microorganisms, such as yeast, mold, microalgae, bacteria, etc., can convert carbohydrates into oil and store them in the bacteria under certain conditions. This oil is called microbial oil. The strains that can accumulate more than 20% of the dry weight of the cells in the cells are called oleaginous microorganisms. The oil content of some oleaginous microorganisms can reach more than 70% of the dry cell weight (Ratledge C. Acta Biotechnology, 1991, 11(5), 429). The fatty acid composition of microbial oil is similar to that of general vegetable oil, mainly C16 and C18 series fatty acids, such as palmitic acid, stearic acid, oleic acid a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
Inventor 赵宗保靳国杰杨帆
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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