147 results about "Medicago" patented technology

The present invention relates to a process for cross-linking proteins, according to which the crosslinking agent is a ketose containing 3 to 5 carbon atoms and said proteins are chosen from the group comprising proteins from animal tissues, from milk or from blood, such as in particular casein, gelatin or collagen; proteins from cereals, such as in particular maize, wheat or rice proteins; protein from high-protein plants, such as in particular pea, alfalfa, lupin, barley, millet or sorghum proteins; proteins from oleaginous plants, such as in particular soybean proteins, for instance soybean cakes, rapeseed or flax proteins, for instance rapeseed cakes, sunflower, groundnut or cotton proteins; and proteins from tubers, such as in particular from potato or manioc.

The invention discloses a method for planting transplanting type alfalfa. The method for planting the transplanting type alfalfa comprises the following steps that alfalfa varieties which are strong in disease resistance and early in dormancy are selected, seeds are selected through the winnowing and screening modes, and the seeds are spread and placed under the blazing sun for 3-4 days; a land which is leeward, exposed to the sun, high in terrain, dry in soil, good in water draining and close to a water source is selected to serve as a seedbed land; the sowing time is selected between March and April, a nutrition pot is used for growing seedlings, the seeding rate is 20 grams per square meter, and seeds are covered by soil by 2 centimeters; water is supplemented and covering is carried out timely according to the soil water regime of a seedbed; transplanting is carried out when the rainy season comes in mid-to-late June, the transplanting method of digging dibble water sitting sowing or digging pits is adopted, or transplanting is carried out before rainy days or after rainy days; weeds around rice seedlings are removed at the time of soil freezing and melting in the late autumn and early winter, in the late autumn and early winter, the land is irrigated once with frozen water and is irrigated with green resuming water when the alfalfa begins to return to green every spring; the alfalfa is not harvested in the year when the alfalfa is transplanted to enable the alfalfa to live through the winter safely, and the alfalfa can be harvested in autumn next year. The method is simple and convenient to operate, and the yield of the alfalfa is improved.

The invention discloses a medicago pseudomonas strain capable of producing ACC (1-aminocyclopropane-1-carboxylate) deaminase and application of the medicago pseudomonas strain, and relates to a medicago pseudomonas strain and application thereof. The pseudomonas (pseudomonas sp.) MJM-9 is preserved in the general microorganism center of the China microorganism culture preservation management committee in 26th July, 2012, and the preservation number of the pseudomonas MJM-9 is CGMCC NO.6294. The medicago pseudomonas strain containing the ACC deaminase is used as bacterial manure for growing crops. The medicago pseudomonas strain can generate the ACC deaminase, secrete auximone IAA (indole acetic acid), synthesize siderophores, reduce the concentration of ethylene inside a plant under the condition of environmental stress, promote the plant to grow fast, particularly promote growth and development of a root system of the plant, and increase utilization rate of fertilizers. Besides, when the strain is applied to agricultural production, growth of plants can be promoted, yield of crops can be increased, and stress resistance of the crops is improved.

The invention discloses a soft-shelled turtle feed for preventing and treating parotitis as well as a preparation method thereof. The soft-shelled turtle feed for preventing and treating parotitis is prepared from the following raw materials in parts by weight: 20-30 parts of sweet corn protein powder, 10-15 parts of wheat germ powder, 5-10 parts of sesame seed meal, 10-15 parts of alfalfa hay, 8-12 parts of wheat middling, 30-40 parts of marine fish powder, 6-9 parts of snail powder, 3-5 parts of dry sea sedge, 2-3 parts of radices trichosanthis, 1-2 parts of chrysanthemum leaves, 2-3 parts of plantain herb, 0.5-1.5 parts of centella, 1-2 parts of phaseolus radiates, 1.5-2.5 parts of hibiscus mutabilis, 2-3 parts of purslane and 1-2 parts of phagostimulant. The chrysanthemum leaves, plantain herb, centella, phaseolus radiates, hibiscus mutabilis and purslane are added to the feed provided by the invention on the basis of guaranteeing the nutrition requirement of soft-shelled turtle growth, and can play effects of clearing away heat and detoxifying, dispelling wind, removing stasis, fighting bacterium, diminishing inflammation, relieving pain and stopping blood, improve the disease resistance of soft-shelled turtles, can effectively prevent and treat parotitis of soft-shelled turtles, guarantee the healthy growth of soft-shelled turtles, improve the survival rate of soft-shelled turtles, reduce the cultivation hazard, and improve the economical benefit of farmers.

The invention relates to the field of mining area ecological restoration, in particular to a limestone mining area ecological restoration method. The method includes the steps of mining area base material treatment and ecological restoration layer building. According to the method, limestone mining area slag is used as a main base material which is supplemented by a little loam, an ecological organic fertilizer and a mycorrhizal fungus inoculant to provide stable nutrient supply for germination and growth of plants, so that a material guarantee is provided for mining area ecological restoration; clover is drought-resistant and alkali-resistant and can be used as a nitrogen-fixing plant for soil improvement at the same time, lavender is used as an ecological restoration plant, which not only restores the ecological environment of a limestone mining area, but can be an ornamental and commercial crop to bring added values, and with collaborative planting of the clover and the lavender, not only can the ecological environment of the limestone mining area be improved, but the soil quality of the area can be improved, so that continuous restoration of the ecological environment of the mining area is guaranteed.

The present invention is rapid identification of a powdery mildew gene of Medicago truncatula, relates to discipline knowledge of plant comparative genomics, genetics and bioinformatics and belongs to the technical field of plant biotechnology. The invention mainly comprises the following steps: 1) download of Medicago truncatula genome sequence and collection of an MLO gene; 2) identification of MLO type gene; 3) MLO gene phylogenetic relationship; and 4) comparison of MLO type powdery mildew gene. The invention effectively shortens a mining cycle of the powdery mildew gene of Medicago truncatula and facilitates the rapid identification of the powdery mildew gene. The identified candidate powdery mildew genes can be used to develop corresponding coseparation functional markers (SNP and SCAR, etc.), and can also be used quickly for molecular marker assisted selection of powdery mildew resistant gene, and has high accuracy. Combined with other disease resistant gene molecular markers, the identified candidate powdery mildew genes can be used in development of multiresistance breeding material, so as to shorten the breeding period and improve breeding efficiency. The invention lays foundation to elaborate molecular mechanism of powdery mildew resistance of Medicago truncatula.

An Aids and associated conditions related to Aids treating compositions is disclosed. The compositions comprise: a medicament selected from an extract of at least one of the following plant families: Apocynaceae (Pleioscarpa Bicarpellata); Annonaceae (Cleistopholis Patens); Dichapetalaceae (Dichapetehan Madagasca Riense); Annoceae (Uvaristrum Pierreanum); Cynocynaceae (Strophantus Gratus); Asclepiadaceae (Gongronema Latifolium); Combretaceae (Combretum Racemosum); Apocynaceae (Alostonia Boonei); Amaranthaceae (Altemanthera Pungens); Aroceae (Anchomanes Differmis); Cyperaceae (Seleria Voivinil); Anacardiaceae (Lannea Acida); Bignoniaceae (Kigelia Africana); Bombacaceae (Ceiba Pentanota); Anarcardiaceae (Antrocaryon Micraster); Bombacaceae (Bombax Bounopozense): Anarcardiaceae (Spondias Mombin); Caricaceae (Carica Papaya); a glyceryl ester of any of the foregoing extracts; a saponin of any of the foregoing extracts; an alkaloid of any of the foregoing extracts; a protein of any of the foregoing extracts; a fat of any of the foregoing extracts; a sugar of any of the foregoing extracts; and any mixture of any of the foregoing.

The present invention relates to an antifungal protein, AlfAFP1, which is a modified form of an antifungal protein isolated from Medicago plants, the modified form exhibiting enhanced anti-fungal activity for controlling fungal pathogenesis in plants. A method for inhibiting fungal colonization of plants is described which includes preparation of nucleotide sequences encoding the modified antifungal protein, preparation of vectors containing the nucleotide coding sequence, and methods for transforming plants with the nucleotide sequences. The polypeptide can be formulated into compositions useful in controlling plant pathogenic fungi.

The invention discloses a planting method of big peaches with high yield and high quality. The planting method comprises the steps of selecting a sandy soil field in which alfalfa is sown last year and which is high in terrain and good in air permeability, plowing alfalfa into the ground in continuous cloudy and rainy days, then applying farm manures and biological pellet organic and inorganic compound fertilizers, plowing and bleaching; digging soil in the field after bleaching, so as to form planting pits; uniformly mixing a seaweed continuous cropping agent, calcium superphosphate, soil and water, so as to obtain mulching liquid; putting roots of big peach tree seedlings into the mulching liquid, soaking, then taking out, and planting in the planting pits; subsequently, backfilling soil, treading, watering and stabilizing the seedlings; after planting, taking field management measures including (A) interplanting management, (B) branch trimming, (C) management of flowers and fruits, (D) fertilizer management and (E) pest control; picking undamaged fruits without diseases and pests or wounds after flowering for a certain time, and storing. The big peaches produced by using the planting method have the advantages of compact flesh, rich nutrition, crispy taste, high yield and good quality.

The invention relates to an amplifying nucleic acid primer for rapidly detecting phytophthora. medicaginis at normal temperature. The LAMP amplifying nucleic acid primer comprises an FIP primer, an F3 primer, a BIP primer, a B3 primer, an LF primer and an LB primer. The LAMP detecting system of the invention has favorable specificity and can rapidly, conveniently and efficiently detect phytophthora. medicaginis under the condition of 63 DEG C and meet the rapid detecting requirement of a port on the phytophthora. medicaginis.

The present invention discloses a pig feeding attractant, which is prepared from the following raw materials by weight: 5-15 parts of pericarpium citri reticulatae, 5-15 parts of hawthorn, 10-20 parts of red date, 5-10 parts of chinese wolfberry, 2-5 parts of malt, 3-7 parts of medicago, 3-5 parts of licorice root, 2-5 parts of tuckahoe, and 1-4 parts of cinnamomum tamala. The pig feeding attractant of the present invention has a special fragrant and sweet incense so as to completely cover the adverse odor in the feed, and further has effects of feed palatability enhancing, feed intake increasing, and reduction of waste of the feed.

The invention discloses a double-inoculation method for improving plant resistance in heavy metal-contaminated soil. The double-inoculation method comprises the following steps: step 1, culture of a bacterial strain and acquisition of a bacterial suspension, wherein the bacterial strain used in a double-inoculation system is wild rhizobacteria (S.meliloti CCNWSX0020) and plant growth-promoting rhizobacteria gelatinous bacillus (P.Mucilaginosus ACCC10013); step 2, performing germination treatment on alfalfa seeds; step 3, performing double inoculation on rhizobacteria-bacillus on alfalfa. The double-inoculation method disclosed by the invention has the benefits that the toxic effects of heavy metals on plants are alleviated by constructing the double-inoculation method for rhizobacteria andplant growth-promoting rhizobacteria, the plant biomass is increased, the plant resistance and the ability to extract the heavy metals are improved, meanwhile the soil nutrient circulation in a contaminated area is promoted, and a new strategy is provided for bioremediation technology plant management measures for heavy metal-contaminated soil in a mining area.

The invention discloses equipment and a method for applying additional water-soluble fertilizers to medicago. The equipment comprises a framework. The framework comprises a fixed frame and a vertical movable frame, a vertical strip-shaped groove is formed in the fixed frame, and the vertical movable frame is fixedly connected into the strip-shaped groove in such a manner that the vertical movable frame can be longitudinally vertically repositioned; a fertilizer tank is arranged on the fixed frame, travel wheels are arranged at the lower end of the fixed frame, a fertilizer application wheel is arranged at the lower end of the vertical movable frame, and a furrowing disc is arranged between the travel wheels and the fertilizer application wheel and is fixedly connected to the lower side of the vertical movable frame; the fertilizer tank is connected with one end of a water outlet pipe, a plurality of branch flow pipes are connected with the other end of the water outlet pipe and are connected with one end of a fertilizer application pipe by flow pumps, and the other end of the fertilizer application pipe extends onto the fertilizer application wheel. The equipment and the method have the advantages that the medicago is planted in a drill seeding manner, and the additional water-soluble fertilizers can be applied to the medicago by the aid of the equipment for applying the additional water-soluble fertilizers while the medicago is harvested; problems of difficulty in applying additional fertilizers, low fertilizer utilization rate, unstable additional fertilizer application periods and the like in existing medicago planting procedures can be solved by the aid of the equipment and the method.

The invention relates to the technical field of agricultural plantation and in particular discloses a method of improving quality of CaoYuan No.3 Medicago varia martyn via clipping. The method of improving quality of CaoYuan No.3 Medicago varia martyn via clipping comprises the following steps of (1) selecting and preparing a land, (2) sowing, choosing a clean-culturing way, (3) managing the field and (4) clipping. The beneficial effects are that after the clipping, contents of crude protein and ash in the herbage can be improved; nutritive value is enhanced; and crude fiber content is reduced; and palatability is improved.

The invention provides a biological pollination method and application of the biological pollination method to planting wild jujube trees in saline-alkali soil. The biological pollination method includes arranging bee cocoons; building artificial honeycombs; arranging the honeycombs; breeding bee swarms; spraying wall bee attractants; releasing bees and withdrawing the honeycombs. The spray quantities of the sprayed wall bee attractants are 2.8L per mu in each procedure. The wall bee attractants comprise, by weight, 7-8 parts of benzyl alcohol, 10-12 parts of veratric acid, 4-6 parts of lavender extracting liquor, 10-12 parts of gamma-nonalactone, 4-6 parts of maltose, 3-5 parts of gardenia extracting liquor and 7-8 parts of clover blossom extracting liquor. The biological pollination method and the application have the advantages that the wild jujube trees can be pollinated by the aid of the wall bee attractants, and the bee attracting quantities are 0.921-0.968 per square meter in wild jujube tree planting areas in pollution periods; the pollination rates of the wild jujube trees can reach 92.0-94.2%; the fruit-setting ratios of the wild jujube trees can reach 89.8-92.3%.

The invention discloses a method for breeding a snail and interplanting medicago in a kiwi fruit garden, which comprises the following steps: constructing a kiwi fruit shelf, and reshaping and trimming a kiwi fruit vine into a shape of single stem and double arms; interplanting medicago in the second year after kiwi fruit is planted, breeding snail after the medicago is interplanted, mixing sand, soil and slaked lime according to a volume ratio of 5:5 to 10:1, and spreading the mixture to the medicago field, feeding feed at dusk every two days, wherein the breeding density is 120 to 200 snails per square meter. According to the method, by planting the medicago and breeding the snail rationally in the kiwi fruit garden, on one hand, the kiwi fruit vine provides a shady environment for the snail, on the other hand, the planted medicago can be eaten by the snail, so that the food intake of the snail and the feeding cost can be reduced, and the utilization rate of the field is improved.

The invention provides an organic cultivation method of intercropping medicago hispida in myrica rubra forest. The organic cultivation method is characterized by comprising the following steps: (1) orchard site selection, (2) base fertilizer applying and soil preparation, (3) sowing, (4) field management: after emerging of the medicago hispida, spreading plant ash in an orchard, wherein the using amount of the plant ash is 40 to 50kg per mu; finely spraying and slowly irrigating during a seeding stage and a seedling stage with the extent that no obvious earth surface runoff appears each time; removing weed in the medicago hispida orchard manually; naturally controlling insect attack by using numerous natural enemies in low mountains and hills; (5) harvesting: harvesting the medicago hispida for several times in one growing season, wherein the harvesting should be flexibly carried out according to the growing trend and market supply and demand situation. Compared with the prior art, the organic cultivation method provided by the invention has the advantages that the land utilization rate is high and comprehensive economic benefit is high.

The invention relates to a water and fertilizer regulation and control method for improving the yield of an alfalfa tame pasture of a newly-reclaimed sloping field at a river valley in an arid region, and the method mainly aims at the problems that the alfalfa tame pasture of the newly-reclaimed sloping field at the river valley in the arid region is large in slope, is thin in soil layer and is poor in water holding and fertilizer retaining capability and the yield is caused by the reduction of soil fertility to be unstable because water and soil loss is liable to happen. Through employing small-water-flow multi-frequency intermittent sprinkling irrigation and combining with fertilization management, the method reasonably optimizes the water and fertilizer application steps and mod. The method enables the water holding and fertilizer retaining of the soil of the alfalfa tame pasture of a thin soil sloping field at the river valley in the arid region to be fully coupled to water and fertilizer application through scientific water and fertilizer regulation and management. The method increases the effectiveness of water and fertilizer, prevents water and soil loss, guarantees the demands of alfalfa for water and fertilizer, and achieves the water and soil retaining and water saving stable yield of the alfalfa tame pasture. The method is suitable for the newly-reclaimed sloping field at the river valley in the arid region, especially for the yield increasing and stabilizing of the alfalfa tame pasture of the thin soil sloping field at the river valley in the arid region, wherein the slope is greater than 2%, and the thickness of the soil layer is 20cm-50cm.

The invention discloses medicago hispida hydroponic nutrient solution. The nutrient solution is composed of lots of elements, trace elements and water, wherein the lots of elements of the nutrient solution comprise carbon, hydrogen, oxygen, sulphur, nitrogen, phosphorus, potassium, calcium and magnesium; the trace elements comprise iron, boron, manganese, zinc, copper, molybdenum, nickel, lead and sodium; the medicago hispida hydroponic nutrient solution contains ammonium sulphate, magnesium sulfate heptahydrate, calcium nitrate terahydrate, ferrisodium, monopotassium phosphate, potassium sulphate, ammonium nitrate, potassium nitrate, ammonium dihydrogen phosphate, borax, manganese sulphate, zinc sulphate heptahydrate, copper sulfate pentahydrate, ammonium molybdate, nickel chloride, lead acetate, calcium carbonate, sodium chloride, zinc disodium ethylenediamine tetraacetic acid and calcium ammonium nitrate. The medicago hispida hydroponic nutrient solution is simple in nutrition ingredients, convenient to prepare, low in preparation cost, and with a certain promotion practical value.

The invention provides a stevia rebaudiana Bertoni stalk and alfalfa mixed and wrapped silage feed and a preparation method thereof. The method comprises the following steps of (1) harvesting raw materials: recovering utilized abandoned stevia rebaudiana Bertoni stalks, and harvesting alfalfa from the squaring stage to the early flowering stage; (2) performing kneading, cutting up and mixing: uniformly mixing the harvested stevia rebaudiana Bertoni stalks with the harvested alfalfa, and performing kneading and cutting up through machinery; (3) performing binding: performing high-density compacting and binding on the cut-up silage raw materials; (4) performing wrapping: performing wrapping and sealing on the bound silage raw materials; and (5) performing storage fermentation. According to the stevia rebaudiana Bertoni stalk and alfalfa mixed and wrapped silage feed disclosed by the invention, the stevia rebaudiana Bertoni stalks and the alfalfa are selected as silage raw materials for mixed silage, so that the problem that the alfalfa is low in soluble carbohydrate content, high in buffering energy and difficult in direct ensile can be solved; besides, stevia rebaudiana Bertoni waste residues, stevia rebaudiana Bertoni stalks and the like are also utilized, so that resource waste is reduced, and environmental pollution is reduced; and besides, the source of the silage feed is extended, and the nutrient quality of the silage raw materials is guaranteed.

The invention provides a traditional Chinese medicine for treating tubercular meningitis, which comprises the following raw materials: cortex phellodendri, franzenbach rhubarb root and rhizome, radix angelicae, gardenia, eclipta, semen euryales, bombyx batryticatus, rhodiola rosea, aster ageratoides, debregeasia orientalis, moellendorf's spidemoss herb, medicago falcate, radix codonopsis, radix isatidis, coptidis rhizome, meadowrue root and rhizome, lesser chickweed, saposhnicovia divaricata and vine of multiflower knotweed. The traditional Chinese medicine for treating tubercular meningitis can reduce side effects during treatment, reduces drug reaction, improves the treatment effect and is worth clinic further discussion and popularization.

The invention discloses a composite improvement method for reclamation soil of a semi-arid mining area. The method comprises green manure, a microbial mixed inoculant and a water-retaining agent, wherein the green manure is alfalfa, and the microbial mixed inoculant is formed by mixing trichoderma, bacillus subtilis and bacillus mucilaginosus according to the proportion of 1: 2.5: 2.5. Harvested alfalfa is cut up, dried in the shade and mixed with the microbial mixed inoculant according to a certain proportion to obtain a biological fertilizer; then strip-shaped turning and pressing are conducted on reclamation soil of the semi-arid mining area, and a biological fertilizer layer is formed; a water-retaining layer is constructed on the biological fertilizer layer, so that the function of the biological fertilizer can be promoted by the water-retaining agent, the physical and chemical properties of the soil are improved by the water-retaining agent and the biological fertilizer which aresynergistic, the biological activity of the soil is improved, the water retention rate of the soil and the contents of organic matters and total nitrogen are effectively improved, the phosphorus andpotassium activation of soil fixation is promoted, and the construction of organic and efficient high-quality soil is quickly realized for mine reclamation land; meanwhile, the method has the advantages of convenience in material taking, low cost, simplicity and feasibility in operation, no ecological risk and the like.

The invention discloses a multifunctional potato pulp residue grass carp feed. The multifunctional potato pulp residue grass carp feed is characterized by being prepared from the following raw materials in parts by weight: 4-6 parts of cuttlefish meal, 5-7 parts of shrimp meal, 12-14 parts of blood worm meal, 10-13 parts of robinia pseudoacacia leaves, 8-12 parts of medicago, 13-15 parts of germinated soybeans, 2.1-3.4 parts of pepper seed powder, 14-16 parts of semen sesami nigrum powder, 3-7 parts of horn pepper, 4-6 parts of locust tree flower powder, 3-5 parts of chestnut flower powder, 18-23 parts of potato pulp residue, 9-12 parts of onion soaked in vinegar, 20-25 parts of wheat bran, 19-23 parts of sorghum flour, 1-3 parts of sunflower oil, 1-2 parts of table salt, 0.4-0.8 part of dandelion, 0.2-0.3 of lawn pennywort herb, 0.5-0.8 part of orange peel, 3-6 parts of additive and a proper amount of water. According to the multifunctional potato pulp residue grass carp feed, since the potato pulp residue is added into the feed, waste is effectively utilized and cost is saved, and moreover the product cohesiveness is improved; the feed hardly disperses after being put into water, thus protecting the water quality of a water body; meanwhile, various active ingredients are combined, and the prepared feed has the advantages of comprehensive nutrition, good palatability, capability of improving fish body immunity and the like.

The invention relates to a Chinese medicinal preparation for treating urinary calculus and a preparation method. The raw materials of active ingredients for preparing the Chinese medicinal preparation comprise longhairy antenoron herb, common burreed rhizome, stem and leaf of assan crotalaria, barbed skullcap herb, liquoric root, plantain herb, pyrrosia leaf, gardenia, akebia stem, lalang grass rhizome, medicago, rust-coloured crotalaria herb with root, membrane of goose gizzard, humulus japonicus, laver and red phaseolus beans. The Chinese medicinal preparation has the effects of promoting blood circulation to remove blood stasis, clearing heat, alleviating water retention, removing urinary calculus and removing qi stagnation, has an obvious therapeutic effect on urinary calculus removal of each part in the urinary system, has a good treatment effect on obstruction and infection of the urinary system such as waist and abdomen colic pain, hematuria, frequent micturition, urgent micturition and pain in urination caused by calculus, can regulate and restore the function of organs such as the liver, the kidney and the spleen, has no toxic or side effect, has great clinical significance to treatment of the calculus of the urinary system and is safe, and is worthy of being widely generalized and applied.

The invention relates to an organic fertilizer, in particular to an organic fertilizer for planting flos rosae rugosae and a method for preparing the organic fertilizer. The organic fertilizer for planting the flos rosae rugosae comprises, by weight, 28-49 parts of thoroughly decomposed feces, 10-18 parts of closterium powder, 12-20 parts of polysiphonia urceolata, 11-16 parts of astragalus sinicus, 5-13 parts of medicago, 8-16 parts of lotus root skin, 5-17 parts of trace elements, 5-14 parts of litsea cubeba, 5-15 parts of radix isatidis decoction dregs, 5-15 parts of sweet potatoes, 1-6 parts of urea, 2-6 parts of calcium superphosphate and 2-5 parts of multiplex floras. The organic fertilizer for planting the flos rosae rugosae and the method have the advantages that fertilizers prepared from the organic fertilizer for planting the flos rosae rugosae can be directly applied, procedures for preparing the organic fertilizer are simple, and the organic fertilizer is high in utilization rate.

The invention discloses a method for constructing a mixed sowing grassland by reseeding alfalfa in a slightly degraded leymus chinensis meadow, and relates to a method for constructing an artificial grassland in a degraded leymus chinensis meadow. The invention aims to improve the community structure of the leymus chinensis meadow and improve the yield and quality of forage grass. The method comprises the following steps that 1, in early July, the slightly degraded leymus chinensis meadow is cut, stubbles of 3-5 cm are reserved, and light raking operation is conducted; 2, after light raking operation, alfalfa seeds are sown, the sowing rate is 10 kg / hectare, and after sowing, a compacting machine is used for slight compacting; 3, in the current year of alfalfa sowing, the grassland is forbidden to be cut, the grassland is cut twice every year from the next year, the cutting time is selected at the initial flowering stage of alfalfa each time, and after the first cutting, the grassland is fertilized. According to the method, the yield of high-quality forage grass can be increased by 1.5-2 tons / hectare per hectare, the price of the forage grass is 1000 yuan / ton, the income can be increased by at least 1500 yuan / hectare every year, and the economic benefit in the later period is very considerable. The invention belongs to the field of degraded grassland recovery.

The invention relates to a cattle feed. The cattle feed is prepared from the following components in parts by mass: 30-50 parts of corns, 10-20 parts of wheat bran, 15-25 parts of bean dreg, 17-24 parts of cottonseed cakes, 4-6 parts of bone powder, 8-9 parts of fish meal, 8-15 parts of brassica oleracea, 9-12 parts of carrots, 7-9 parts of medicago, 9-11 parts of traditional Chinese medicine mixture and 6-8 parts of table salt. Compared with prior art, the cattle feed prepared by the invention greatly improves qualities of the beef; and the beef has the advantages of good taste and rich nutrition.

The invention discloses a sebastes schlegeli calreticulin gene and an application. The sebastes schlegeli calreticulin gene is an arbitrary one of DNA molecules as shown in (a1) to (a4): a DNA molecule having a coding region as shown in SEQ ID NO.1; (a2) a DNA molecule as shown in SEQ ID NO.3; (a3) a DNA molecule which is hybridized with the DNA sequence defined by (a1) or (a2) under a strict condition and codes the protein in claim 1; and (a4) a DNA molecule which is sourced from medicago truncatula and has more than 90 percent of homology with the DNA sequence defined by (a1) or (a2) or (a3). The sebastes schlegeli calreticulin gene has the advantages of short cycle, simple operation, low price, strong specificity and the like, is economical, and provides technical support to amplification of CRT genes of other species.