The invention relates to an application of a
Zea mays grain
transcription factor in the aspect of regulation and control of alcohal-soluble proteins. The factor comprises a
base sequence shown in SEQ ID NO: 1. The
protein ZmbZIP22 encoded by the sequence can be directly bonded to a 27kDa gamma-
gliadin promoter and activate the 27kDa gamma-
gliadin promoter.
Gene-
deficient mutant plants are obtained through carrying out
Zea mays immature-
embryo conversion by taking a
gene fragment, shown in SEQ ID NO: 2, of ZmbZIP22 as a
guide RNA by using a
CRISPR-Cas9 technology. Compared with
wild type grains, transgenic mutants have
Zea mays grains with irregular and relative-thin
protein body shells, the content of alcohal-soluble proteins in mature grains is remarkably lowered, the content of essential amino acids such as
lysine, which are deficient to the conventional Zea mays, in the mature grains is remarkably increased, and thus, the
nutritional quality of Zea mays is improved.