200 results about "Root nodule" patented technology

The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.

The invention belongs to the technical field of agriculture and environmental pollution improvement and relates to a heavy metal resistant plant endogenous nodule bacterium and application thereof. The heavy metal resistant nodule bacterium is preserved at the China center for type culture collection (CCTCC) with the preservation date to be 2012/9/18 and culture preservation number to be CCTCC NO:M2012357. The bacterial strain liquid preparation contains more than a billion of effective living bacteria per milliliter, and the bacterial strain solid preparation contains 0.2 billion of effective living bacteria per gram. The heavy metal resistant nodule bacterium strain can secrete plant hormones indole acetic acid (IAA) and siderophores, and resists heavy metal copper and cadmium. When plants are planted in moist soil or tailings containing heavy metal and bioremediation preparation is inoculated, the heavy metal resistant nodule bacterium can promote plant growth and absorbs heavy metal copper, can improve plant restoration efficiency, can fix sandy soil and reduces water and soil loss.

The invention relates to an interplant method of camellia oleifera and torreya grandis young growth. The interplant method of the camellia oleifera and the torreya grandis young growth is characterized in that peanut and upland rice are interplanted at intervals between the camellia oleifera and the torreya grandis young growth, and the peanut and the upland rice interchange positions in the nest year. The interplant method of the camellia oleifera and the torreya grandis young growth has the advantages that the upland rice provides shade for the camellia oleifera or the torreya grandis so that expense of sheltering nets is reduced, interplanted the upland rice and the peanut can store water and keep moisture for the torreya grandis young growth so as to avoid water and soil loss, the peanut provides nitrogen needed during in the early stage of production of the camellia oleifera by means of a biological nitrogen fixation function of the nodule bacteria so that nitrogenous fertilizer is saved, intercropping and crop rotation of the interplanted crop can not only avoid damage, caused by continuous cropping, to the soil but also have functions of restraining occurrence and spread of plant diseases and insect pests and isolation, tend is replaced with cultivation, weeds are restrained, management cost of the camellia oleifera or the torreya grandis is reduced, the utilization rate and the copping index of the forest land are improved and economic benefits are increased.

The invention relates to a compound microbial preparation for promoting paddy growth and a preparation method thereof. The compound microbial preparation for promoting paddy growth is characterized by consisting of 20-40 parts by weight of a microbial strain culture and 60-80 parts by weight of a microbial carrier; wherein, the microbial strain culture consists of the following raw materials in parts by weight: 2-8 parts of bacilli subtilis, 2-8 parts of bacilli megaterium, 5-10 parts of saccharomyces cerevisiae and 1-4 parts of rhizobia; the microbial carrier is zeolite or bentonite or mixture thereof. The compound microbial preparation has the advantages of promoting growth of beneficial microbes, enhancing absorption of nitrogen, phosphorous, potassium and other organic substances for paddy root system and promoting normal growth of the root system, thus promoting the paddy growth and reducing chemical fertilizer application.

The invention discloses a phosphorus transportprotein gene GmPT5 related to phosphorus transport of soybean nodulation and application thereof. The phosphorus transportprotein gene GmPT5 related to the phosphorus transport of the soybean nodulation has a nucleotide sequence as shown in SEQ ID NO:1, and coded protein nucleotide sequence is shown as SEQ ID NO:2. The gene GmPT5 regulates and controls the phosphorus transport from a root part to the nodulation, furthermore, the overexpression of the gene is favorable for increasing the size of the soybean nodulation and the plant biomass as well as the nitrogen and phosphorus contents.

The invention discloses a method for remediating polycyclic aromatic hydrocarbon (PAH) polluted soil through cooperation of alfalfa and complex microbial inoculants. Alfalfa seeds are pretreated, and the plant sprouting period is shortened by 2-3 days, so that the tolerance of the plant to soil containing PAHs is enhanced, and the remediating capacity of the plant on the soil is improved. Besides, in an alfalfa-trichoderma-nodule bacterium combined remediating system, the synergistic effect of trichoderma and nodule bacteria is used for promoting degradation of the alfalfa to the PAHs in the polluted soil. Meanwhile, the physiological contour of the microbial population level of alfalfa rhizosphere soil is changed, and the diversity and the stability of the microbial ecological functions of the soil are recovered, so that the growth environment of crops is improved, and the quality and the yield of the crops are improved; the method has wide market application prospects.

The invention relates to a method for improving a plant planting desert, which is realized by the following steps: adding 140 to 170kg of liquid film, 0.6 to 8kg of calcium magnesium phosphate, 0.01 to 0.2kg of ammonium molybdate and 0.4 to 5kg of nitragin of this family into 800 to 1300kg of water, stirring, dissolving, then adding 4 to 50kg of leguminous forage seed, stirring evenly, sowing on the sand of one hectare and enclosing and banning grazing for 3 to 5 years; and sowing 7 to 30kg of gramineous forage grass seed on the sand after the primary period, relieving enclosure and grazing banning after enclosing and banning grazing for 2 to 3 years and keeping the pasturage strength at 5 to 10 animals/year to achieve the ecological equilibrium of animals and plants so as to finish the improvement of the desert. By using the method, the usability of soil can be increased, the soil obtains sufficient water and nutrients, a woodland with large area can become a source of forest, the condition that the growth rate of the forest is greater than the exploitation rate is kept, the forest provides more oxygen gas used by organisms and assists to lower a warming phenomenon, buildings can be constructed again in part of regions with desertification reversion by the windproof protection of the forest, and the volume and the utilization rate of the soil are increased.

The invention relates to a composite microbial preparation for promoting the growth of rape and a preparation method thereof. The composite microbial preparation for promoting the growth of the rape is characterized by comprising 20-40 parts by weight of strain culturing substance and 60-80 parts by weight of microbial carrier, wherein the strain culturing substance comprises the following raw materials in parts by weight: 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium, 5-10 parts of rhodopseudomonas palustris and 1-4 parts of azotobacter chroococcum. The preparation method of the composite microbial preparation for promoting the growth of the rape comprises the following steps: culturing 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium and the rhodopseudomonas palustris together, culturing the azotobacter chroococcum independently, mixing and fermenting. The invention has the advantages of promoting the growth and the propagation of beneficial microorganisms, increasing the absorption of the root system of the rape to nitrogen, phosphorus, potassium and other organic substances and promoting the normal development of the root system, thus promoting the growth of the rape and reducing the applied chemical fertilizer.

The invention provides rhizobium Scot1305 and a symbiotic system of rhizobium Scot1305 and plants is established. Due to nitrogen fixation function, siderophore secretion function and soluble phosphorus activity of the rhizobium strain and protection effect of the rhizobium strain on key enzyme of plant tocopherol synthesis, enhancement effect of the strain on phytoremediation of Co contaminated soil is realized. The strain can coexist with plants in Co contaminated soil, and can promote the extraction and enrichment of heavy metals Co in the aerial parts of plants, and finally achieve the purpose that the content of Co in soil meets the environmental safety standard.

The invention discloses a method for repairing arsenium-polluted farmland soil through microorganisms and plants in a combined manner. The method includes the following steps that firstly, a microbial agent is sprayed, wherein nodule bacteria are sprayed in the arsenium-polluted farmland soil after being dissolved in water; secondly, plant plantation is conducted, wherein Nephrolepiscordifolia(L.)Presl is planted in the arsenium-polluted farmland soil where the nodule bacteria are sprayed; and thirdly, field management is conducted, wherein the field management is conducted on the Nephrolepiscordifolia(L.)Presl, and normal growth of the plants is ensured. Treatment of arsenium-polluted farmland soil repair is conducted through the microorganisms and the plant repair technology in a combined manner, the nodule bacterium NT-26 and Nephrolepiscordifolia(L.)Presl combined repair is adopted, the leaching concentration of the heavy metal arsenium in the repaired and treated farmland soil is smaller than the IV-type standard in Quality Standard for Ground Water (GB/T14848-93), the whole construction process is high in benefit, and influences on the environment are small.

The invention discloses a rhizobium strain and its application. The rhizobium strain is named as Rhizobium sp., the strain number is zb612, and preservation number is CGMCC No. 10721. The rhizobium strain was preserved in the China General Microbiological Culture Collection Center (CGMCC) at the No.3, 1 West Beichen Road, Chaoyang District of Beijing on April 15th, 2015. The strain provided by the invention can be used in onsite real nitrogen removal from sewage so as to raise the effect of removing ammonia nitrogen from oil sludge. The rhizobium provided by the invention has the characteristic of removing ammonia nitrogen. According to the rhizobium strain, organic carbon can be used as the sole carbon source and ammonia nitrogen is used as the sole nitrogen source so as to carry out metabolism, and ammonia nitrogen is directly converted to N2 through heterotrophic nitrification-aerobic denitrification so as to finish nitrogen removal. Thus, problems of aerobiotic nitrification and anoxic denitrification sectional treatment in traditional biological removal of nitrogen are solved.

The invention discloses a microecological modulator. The microecological modulator is prepared from photosynthetic bacteria, actinomycetes, bacillus, saccharomycetes, lactic acid bacteria, nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium bacteria, silicate bacteria, bacillus radicicola, blue-green algae, vitamin C, nitrogen, boron, iron, saccharose, calcium sulfate, dipotassium phosphate, calcium carbonate, magnesium sulfate, sodium chloride and agar. According to the microecological modulator disclosed by the invention, by adopting a fusion technique of multiple useful microbiological populations, the useful microbiological population and plant enzymes are fermented and cultivated at the same time, mutual phagocytosis of biological populations in a fusion process of the biological populations can be prevented, and symbiosis and compatibility are realized. The microecological modulator disclosed by the invention is rich in multiple microbiological populations and multiple trace elements and mineral substances needed by plant growth, so that microecological circulation of soil can be improved, and the fertilizer efficiency of the soil is enhanced; the microecological modulator replaces chemical fertilizer; photosynthesis of leaf surfaces of plants and crops is enhanced, the oxidation is reduced, and leaf surfaces of the plants and the crops can be lustrous and plump; the plants and the crops can be full in fruits; the yields of the plants and the crops can be increased.

The invention relates to a nodule bacteria transferring method by utilizing distant grafting, comprising: 1) hybrid sunflowers and corns which have high plasticity are chosen as stock; 2) varieties with high nitrogen fixation activity and stable hereditary are chosen from leguminous plants; 3) cleft grafting is adopted, when 1-6 pieces of leaves of the stock grow and 1-6 pieces of leaves of the scion grow, grafting is carried out; 4) after the grafting survival is realized, the growth amount of the stock and the scion is rationally controlled; the plants with nodule bacteria and the plants without nodule bacteria are grafted by utilizing the distant grafting, so as to lead the nodule bacteria to be transferred to the plants without nodule bacteria and cause the plant without nodule bacteria to have nodule bacteria naturally.