32 results about "Clonostachys rosea" patented technology

A process and method for the production of endophytes as plant inoculant products, specifically Clonostachys rosea strain 88-710, for the promotion of plant vigor, health, growth and yield are disclosed. The endophyte, Clonostachys rosea strain 88-710 produces a fungal conidial preparation by utilizing a discrete solid substrate fermentation system, namely Potato Dextrose Agar or Malt Extract Agar. Additionally, the endophyte, Clonostachys rosea strain 88-710, can act as an inoculant to stimulate and have an additive effect with rhizobium bacteria on the production of nitrogen fixing nodules on legumes and growth enhancement e.g. beans, soybeans, peas and alfalfa. As well, Clonostachys rosea strain 88-710, can combine with rooting hormones, e.g. indole-3-butyric acid (IBA) to provide inoculant and rooting benefits to cuttings / transplants of plants.

Clonostachys rosea strains have novel usefulness as inoculants of plants promoting plant vigor, health, growth, yield and a reduction of competitive stress caused by other fungi when used alone or sequentially with many fungicides in an integrated pest management system (IPM). Seed and foliar uses are shown to inoculate and subsequently achieve endophytic colonization of the portion of the plant treated. While the germinating conidia of this organism has been shown to tolerate several fungicide groups, the established mycelium of Clonostachys rosea is significantly more tolerant to systemic fungicides facilitating use in seed and foliar applications. Seed treatment and colonization may occur at any time after harvest resulting in endophyte enhanced seed that ignores or is marginally altered by other fungal organisms and may be suitable for Feed, Food and Seed uses.

The present invention relates to a fungi mixing bacterial agent for preventing and controlling plant meloidogyne diseases, and applications thereof, wherein two fungi cultures such as Purpureocillium lilacinum CGMCC No.9344 and Clonostachys rosea CGMCC No.1037 are cultured to prepare the fungi mixing bacterial agent. According to the present invention, the fungi mixing bacterial agent can be mixed with seedling breeding mediums, organic fertilizers and organic-inorganic compound fertilizers or be used separately so as to perform soil treatment or hole application before or after transplantation, or root-irrigation, and is used for preventing and controlling plant meloidogyne diseases.

The invention discloses clonostachys rosea and a solid fermenting agent and application thereof, and aims at solving the technical problems of chemical pollution, complicated screening of biological antibacterial bacteria and complicated production process. A clonostachys rosea strain NF-06 with the preservation number of CGMCC No.16262 is screened. The solid fermenting agent is prepared by solidfermentation of the clonostachys rosea strain NF-06. The clonostachys rosea strain NF-06 or the solid fermenting agent is used for controlling plant root knot nematode. The method is simple and easy to operate, has low cost, has high effective viable count of spore powder, is friendly to ecology and safety, has simple production process and low cost, and is beneficial to application and promotion.Less chemical agents can be used.

The invention provides zearalenone degrading enzyme mutants with improved enzyme activity as well as a coding gene and application of the zearalenone degrading enzyme mutants. Zearalenone degrading enzyme genes from Clonostachys rosea are modified by an error-prone PCR method, and the zearalenone degrading enzyme mutants YMDS01, YMDS02 and YMDS03 are obtained after screening. The zearalenone degradation capability of the zearalenone degrading enzyme mutants is obviously improved, and the zearalenone degrading enzyme mutants have good market application prospects and industrial value.

The invention provides clonostachys rosea-bacillus subtilis composite wettable powder containing clonostachys rosea fungus conidial powder for preventing and treating gray mold, sheath blight, root rot, fungal leaf spot and sclerotinia rot and bacillus subtilis bacterial spore powder for preventing and treating bacterial wilt, soft rot diseases, canker, bacterial spot diseases and bacterial angular leaf spot diseases. The wettable powder comprises, by weight, 50-100 parts of clonostachys rosea fungus conidial powder, 50-100 parts of bacillus subtilis bacterial spore powder, 800-850 parts of diatomite, 10-20 parts of sodium dodecyl benzene sulfonate, 10-50 parts of sodium lignosulfonate, 10-50 parts of carboxymethylcellulose sodium and 5-10 parts of ascorbic acid. The wettable powder composite microbes do not produce antagonistic action, have a wide sterilization spectrum, are safe for crops, users and an environment, improve crop quality and improve a yield and performance stability.

The invention discloses a zearalenone hydrolase with improved trypsin resistance. The zearalenone hydrolase is an enzyme which is produced by single amino acid substitution in a zearalenone hydrolase derived from Clonostachys rosea and having an amino acid sequence of SEQ ID NO. 1 and has stronger trypsin resistance, wherein the amino acid substitution is the substitution at the 262 site. The invention provides a zearalenone hydrolase mutant with improved trypsin resistance by modifying key amino acid residues in a wild zearalenone hydrolase molecule through a protein engineering technology. The resistance half-life period of the enzyme to trypsin is prolonged by 20.8% compared with that of the wild zearalenone hydrolase, and other enzymatic properties are basically consistent with those of the wild zearalenone hydrolase.

The present invention discloses a clonostachys rosea engineering strain for transforming chitinase gene, wherein the strain is named Cr-chi32 and has the preservation number of CGMCC No.10027 in the China General Microbiological Culture Collection Center. The engineering strain Cr-chi32 has effects of enhancing of disease resistance of clonostachys rosea and improvement of prevention effect stability, can be effectively used for prevention and control of plant diseases, particularly for prevention and control of Sclerotinia and Botrytis cinerea of crops, and further provides good prevention and control effects on wilt, root rot, and other plant fungal diseases.

The invention relates to a clonostachys rosea (clonostachys rosea) strain YZC3 and an application thereof, and belongs to the technical field of microorganisms. The preservation number of the clonostachys rosea strain YZC3 is CGMCC No.14782. Based on the clonostachys rosea strain YZC3, the invention further provides a product capable of degrading lignin, a microbial agent for degrading the lignin,a product for composting park afforesting waste, a microbial agent for composting the park afforesting waste and a method for composting the park afforesting waste. The clonostachys rosea strain YZC3disclosed by the invention has excellent effects in the respect of degrading the lignin.

The invention discloses a continuous cropping-resistant compound microbial preparation and a preparing method thereof. The continuous cropping-resistant compound microbial preparation is a plant compound microbial agent. An active ingredient of the plant compound microbial agent is composed of streptomyces silaceus and clonostachys rosea. The streptomyces silaceus can be streptomyces silaceus SQ11, and the clonostachys rosea can be clonostachys rosea GR02. The continuous cropping-resistant compound microbial preparation can be prepared by a solid fermentation process, is simple and convenient,does not produce waste water and waste gas, has the advantages of pollution-free production, pure microbial microbial agent, and no addition of any hormones or chemical agents, guarantees the safetyof crop quality and zero pollution to the environment, reduces the production cost of farmers, guarantees the safe quality of agricultural products, and effectively reduces the amount of chemical fertilizers and pesticides.

The invention discloses an engineering strain-clonostachys rosea for perilipin gene transfer and an application of the engineering strain. The engineering strain is named as Cr-per44, has a preservation registration number of CGMCC (China General Microbiological Culture Collection Center) No.10028 in the CGMCC, is obtained by transferring a parasitic related gene-perilipin gene Per3 into clonostachys rosea HLD-1 through protoplast transformation, is strong in disease resistance and stable in prevention effect, can effectively prevent and control sclerotinia and gray mold of crops, and has relatively good prevention and control effects on various plant fungous diseases such as blight and root rot.

The invention discloses a preparation method of biocontrol bacterium immobilized microspheres optimized by a response surface methodology, and belongs to the field of biocontrol bacterium agents. The invention aims to optimize the preparation process of the immobilized bacterium microspheres, and finds the optimal process conditions based on the response surface methodology optimization, so that the effectiveness of the immobilized bacterium microspheres in practical application is improved. The method comprises the following steps of transferring a Clonostachys rosea strain into PDA culture under a sterile condition, transferring the strain subjected to purification culture into a PDB liquid culture medium added with antibiotics, placing the PDB liquid culture medium in a constant-temperature shaking table for shaking culture to obtain Clonostachys rosea fermentation liquor, adding a sodium alginate solution, slowly dropwise adding into a sterilized CaCl2 solution by using an injector, and performing hardening to form the Clonostachys rosea immobilized microspheres. The influence of different conditions on the Clonostachys rosea embedding rate in the embedding process is explored by utilizing the response surface methodology, a continuous variable curved surface model is established, and the optimal embedding condition is fitted and determined through a regression equation, so that the maximum efficacy of microorganisms is exerted.

The invention discloses a method for producing podophyllotoxin by using callus adventitious roots of sinopodophyllum hexandrum royle. The method comprises the steps of obtaining sterile embryos, carrying out embryonic callus induction, adventitious roots growing, induction production of the podophyllotoxin, and the like. The set of method for producing the podophyllotoxin by coupling cell engineering with fermentation engineering is established. The method provided by the invention is simple to operate and high in yield, subculture does not occure to the calluses, browning degree is low; the adventitious roots are treated by using endophyte of the sinopodophyllum hexandrum royle, clonostachys rosea (fhnzm) fermentation liquor and some plant growth regulators, so that the content of the sinopodophyllum hexandrum royle is effectively increased.

The present invention discloses a clonostachys rosea engineering strain for transforming chitinase gene, wherein the strain is named Cr-chi32 and has the preservation number of CGMCC No.10027 in the China General Microbiological Culture Collection Center. The engineering strain Cr-chi32 has effects of enhancing of disease resistance of clonostachys rosea and improvement of prevention effect stability, can be effectively used for prevention and control of plant diseases, particularly for prevention and control of Sclerotinia and Botrytis cinerea of crops, and further provides good prevention and control effects on wilt, root rot, and other plant fungal diseases.

The invention discloses a method for producing podophyllotoxin by using callus adventitious roots of sinopodophyllum hexandrum royle. The method comprises the steps of obtaining sterile embryos, carrying out embryonic callus induction, adventitious roots growing, induction production of the podophyllotoxin, and the like. The set of method for producing the podophyllotoxin by coupling cell engineering with fermentation engineering is established. The method provided by the invention is simple to operate and high in yield, subculture does not occure to the calluses, browning degree is low; the adventitious roots are treated by using endophyte of the sinopodophyllum hexandrum royle, clonostachys rosea (fhnzm) fermentation liquor and some plant growth regulators, so that the content of the sinopodophyllum hexandrum royle is effectively increased.

The invention provides clonostachys rosea-bacillus subtilis composite wettable powder containing clonostachys rosea fungus conidial powder for preventing and treating gray mold, sheath blight, root rot, fungal leaf spot and sclerotinia rot and bacillus subtilis bacterial spore powder for preventing and treating bacterial wilt, soft rot diseases, canker, bacterial spot diseases and bacterial angular leaf spot diseases. The wettable powder comprises, by weight, 50-100 parts of clonostachys rosea fungus conidial powder, 50-100 parts of bacillus subtilis bacterial spore powder, 800-850 parts of diatomite, 10-20 parts of sodium dodecyl benzene sulfonate, 10-50 parts of sodium lignosulfonate, 10-50 parts of carboxymethylcellulose sodium and 5-10 parts of ascorbic acid. The wettable powder composite microbes do not produce antagonistic action, have a wide sterilization spectrum, are safe for crops, users and an environment, improve crop quality and improve a yield and performance stability.

The invention discloses a mildew Mycoplasma pink, its solid fermentation inoculant and its application, and aims to solve the technical problems of chemical pollution control, complicated biocontrol bacteria screening and complicated production process. Screening a strain of Slime sclerotiorum ( Clonostachys rosea ) NF‑06, whose deposit number is CGMCC No.16262. Culturing and preparing a solid fermentation inoculum obtained by solid fermentation of Mycoplasma pink strain NF-06. The Myxoplasma pink strain NF-06 or the solid fermentative inoculum is used in the control of plant root-knot nematode disease. The method of the invention is simple and feasible, low in cost, high in effective viable bacteria count of the spore powder, friendly to ecological safety, less chemical agent is used, simple in production process, low in cost, and beneficial for application and popularization.

The invention relates to Clonostachys rosea, Clonostachys rosea liquid and application of the Clonostachys rosea liquid in prevention and treatment of Sclerotinia sclerotiorum(Lib.)deBary. The collection unit name of the Clonostachys rosea MES is China General Microbiological Culture Collection Center, and the collection number is CGMCC 3.11519. The Clonostachys rosea liquid contains 1.8 * 10<7> to 2.3 * 10<7> Clonostachys rosea spores per mL. The preparation method comprises the following steps of: picking fresh activated and cultured Clonostachys rosea hyphae, inoculating a plate containing a solid PDA (potato dextrose agar) culture medium with the fresh activated and cultured Clonostachys rosea hyphae, and culturing in a constant-temperature incubator at 25-30 DEG C for 12-16 days; and adding 20-30mL of Tween 80-containing sterile water into the culture dish, washing off the spores with a coater, filtering, and carrying out 8-12-time gradient dilution. The Clonostachys rosea liquid provided by the invention has obvious antagonistic effects on sclerotinia sclerotiorum causing Sclerotinia sclerotiorum(Lib.)deBary, and is capable of preventing or relieving the occurrence of Sclerotinia sclerotiorum(Lib.)deBary, promoting stable yield and income increase of sunflowers and improving the yield and quality of sunflowers.

The invention relates to a compound bactericidal composition and application thereof. The compound bactericidal composition comprises spores of Clonostachys rosea and fludioxonil, and the weight ratio of the spores of the Clonostachys rosea to the fludioxonil is (90-99.98):(0.02-10). The compound bactericidal composition provided by the invention is suitable for preventing and treating crop diseases caused by sclerotium rolfsii, such as capsicum southern blight, and can be used for treating soil of a protected land; and the prevention and treatment effect is obviously improved compared with that of a single agent, the dosage is relatively small, the pesticide cost can be reduced, and pollution to the soil environment is reduced.

The invention discloses a compound microbial preparation against heavy cropping and a preparation method thereof. The compound microorganism preparation against repeated cropping is a compound microorganism preparation for plants, and the active ingredients of the composite microorganism preparation for plants are composed of Streptomyces yellow ochreus and Clonospora pink. The Streptomyces silaceus may be Streptomyces silaceus SQ11, and the Clonostachys rosea may be Clonostachys rosea GR02. The anti-stubble compound microbial preparation of the present invention can be prepared by a solid fermentation process, which is simple and convenient, does not produce waste water, waste gas, and is produced without pollution. The pure microbial agent does not add any hormones and chemical preparations, ensuring crop quality safety and zero environmental impact. Pollution, reduce farmers' production costs, ensure the quality and safety of agricultural products, and effectively reduce the amount of chemical fertilizers and pesticides.

The invention relates to a bactericidal composition containing Clonostachys rosea spores and boscalid. The bactericidal composition is prepared from the Clonostachys rosea spores and the boscalid, wherein the weight ratio of the Clonostachys rosea spore powder to the boscalid is (80-99.93):(0.07-20). The sterilization composition provided by the invention is suitable for preventing and treating crop diseases caused by sclerotium rolfsii, such as southern blight, and can be used for treating soil of a protected land. The prevention and treatment effect of the sterilization composition is obviously improved compared with that of a single agent, and the usage amount is relatively small; and the pesticide cost is greatly reduced, and the pollution to the soil environment can be further reduced.

The present invention relates to a fungi mixing bacterial agent for preventing and controlling plant meloidogyne diseases, and applications thereof, wherein two fungi cultures such as Purpureocillium lilacinum CGMCC No.9344 and Clonostachys rosea CGMCC No.1037 are cultured to prepare the fungi mixing bacterial agent. According to the present invention, the fungi mixing bacterial agent can be mixed with seedling breeding mediums, organic fertilizers and organic-inorganic compound fertilizers or be used separately so as to perform soil treatment or hole application before or after transplantation, or root-irrigation, and is used for preventing and controlling plant meloidogyne diseases.

A composition for biologically controlling phytopathogenic fungi that pertains to the agricultural biotechnology area, more particularly a composition of bacteria and fungi with different complementary mechanisms of action in the mitigation of phytopathogenic fungi in crops. The composition is a formulation containing Bacillus amyloliquefaciens and Trichoderma harzianum mixed with additives and excipients. Micro-organism Clonostachys rosea can also be added.