305 results about "Endophyte" patented technology

This invention relates to methods and materials for modulating seed nutritional quality traits of seeds produced by a wheat, cotton, soybean, or maize plant, said plant having been heterologously disposed to, or grown from, a plant element treated with an endophyte.

The invention discloses a rapid propagation method for dendrobii officmalis caulis. The rapid propagation method comprises the following steps: sterilizing the surfaces of seed capsules, sowing the sterilized seeds in a germination culture medium, grafting seedlings germinated by seeds to a protocorm differential medium, transferring differentiated seedlings to a strong seedling rooting medium to finally obtain the tissue culture seedlings of dendrobii officmalis caulis. Compared with the prior art, the method has the advantages that uncracked capsules of dendrobii officmalis caulis are adopted so as to effectively prevent endophytes of dendrobii officmalis caulis from bringing troubles to tissue culture and lower pollution rate; a tri-generation culturing method including seed germination, subculture and strong seedling rooting is adopted, so that the seedling period is greatly shortened and the production cost is lowered; and the cultured tissue culture seedlings are robust, and have developed roots and high survival rate.

This invention relates to methods and materials for modulating the characteristics of a plant, said plant having been heterologously disposed to an endophyte or a plurality of endophytes, or derived from a plant reproductive element heterologously disposed to an endophyte or a plurality of endophytes.

This invention relates to methods and materials for modulating the characteristics of a plant, said plant having been heterologously disposed to an endophyte or a plurality of endophytes, or derived from a plant reproductive element heterologously disposed to an endophyte or a plurality of endophytes.

A process and method for the production of endophytes as plant inoculant products, specifically Clonostachys rosea strain 88-710, for the promotion of plant vigor, health, growth and yield are disclosed. The endophyte, Clonostachys rosea strain 88-710 produces a fungal conidial preparation by utilizing a discrete solid substrate fermentation system, namely Potato Dextrose Agar or Malt Extract Agar. Additionally, the endophyte, Clonostachys rosea strain 88-710, can act as an inoculant to stimulate and have an additive effect with rhizobium bacteria on the production of nitrogen fixing nodules on legumes and growth enhancement e.g. beans, soybeans, peas and alfalfa. As well, Clonostachys rosea strain 88-710, can combine with rooting hormones, e.g. indole-3-butyric acid (IBA) to provide inoculant and rooting benefits to cuttings / transplants of plants.

The invention discloses an application of Bacillus megaterium YJB3 in plant growth promotion and bio-control, and belongs to the field of plant protection. The Bacillus megaterium YJB3 has phosphorusdissolving, nitrogen fixing, ferritin producing and other plant growth promoting functions, and also has a plant pathogen inhibiting bio-control effect. Endophytes are colonized in plants or rhizospheres in a rhizosphere inoculation, blade spray or smear treatment or seed or root soaking manner by using a plant endophyte YJB3 bacterium liquid, so the nutrient level of inoculated plants is improved, the disease resistance is improved, the plant biomass is increased, and the plant growth is promoted. The Bacillus megaterium YJB3 is simple, safe and economic to operate, can reduce the use amountsof pesticides and chemical fertilizers in agricultural production and reduce agricultural non-point source pollution, and is of great significance to improving the ecological environment of farmlandsoil and improving the quality of agricultural products.

The invention provides a method for producing taxol by utilizing Chinese yew seed endophyte. The method for producing the taxol by utilizing the Chinese yew seed endophyte comprises the following steps: sterilizing and inoculating while Chinese yew seeds are taken as explants, then carrying out secondary sterilization, culturing for a period of time, picking endophytic fungi at the periphery of the Chinese yew seeds, placing the endophytic fungi into a PDA solid culture medium, culturing, then picking part of bacterial plaque, placing the picked bacterial plaque into PDA liquid and culturing; filtering ferment with gauze, and extracting filtrate with ethyl acetate; carrying out reduced-pressure distillation at the temperature of 35 DEG C after filtering by adopting a rotary evaporator, and dissolving in a small amount of methyl alcohol, so that a methanol solution containing taxol is obtained. The method for producing the taxol by utilizing the Chinese yew seed endophyte has the advantages that the problems that Chinese yew plants grow slowly and chemical synthesis of the taxol is complex while a fungus culturing technique is applied can be solved, a foundation is provided for industrial production of the taxol by fungal fermentation, the method for producing the taxol by utilizing the Chinese yew seed endophyte is environment-friendly, raw materials are rich, and the problem of high supply shortage of the taxol can be well relieved.

The present invention discloses a method of preparing enzymes in a biomimetic manner and the enzymes capable of eliminating atherosclerosis. A first contribution is as follows: cognitive limitations of probiotics and enzymes in the existing technology are broken through, plant own endophytes and endogenous enzymes are used in the biomimetic manner to produce the enzymes, and active ingredients invarious plants are fully released; a second contribution is as follows: in classifications of plant phyla, classes, orders, families, tribes, genera and species, due to the close endophytes and endogenous enzymes, the plants within the same families can be constrainedly fermented together, or plant characteristic components in the fermentation processes are seriously affected; a third contributionis as follows: based on academic points of eliminating pathogens to strengthen vital qi in ''Confucians' Duties to Their Parents'' and macroscopic thoughts that all the pathogen eliminating methods can be achieved by three methods of sweating, emesis and purgation, food enzymes with functions of the sweating, emesis and purgation are used to replace medicines, and purposes of clearing body metabolic waste are achieved by the three ways of the sweating, emesis and purgation; and a fourth contribution is as follows: the method merges modern technology, reduces production difficulties, shortensproduction cycles, and improves product quality.

The invention discloses peltate yam rhizome endophyte for generating saponinase and application. The peltate yam rhizome endophyte is Aspergillus flavus strain SYfx213.2 and preserved in CGMCC, and the preservation number of the peltate yam rhizome endophyte is CGMCC 11517. The peltate yam rhizome endophyte can be used for industrial fermentation and production of peltate yam rhizome saponinase, the yield of saponinase can be improved, and the market requirement is met.

An endophyte or endophyte culture of N. lolii species is described that, in combination with a host grass does not cause typical symptoms of ryegrass toxicosis in grazing animals and also contains levels of compounds from the class of janthitrems epoxides to individually or in combination protect the host grass from pests or abiotic stresses or both. Uses and methods are also described to produce and characterise the combination as well as alternative uses for compounds from the class of janthitrem epoxide compounds.