Pennisetum purpureum Schum-derived bacillus megaterium and use thereof

Abstract

The invention relates to Pennisetum purpureum Schum-derived bacillus megaterium and a use thereof and belongs to the technical field of microbes. The Pennisetum purpureum Schum-derived bacillus megaterium pp02 has a class name of Bacillus megaterium pp02, is preserved in the China general microbiological culture collection center (CGMCC) and has a preservation number of CGMCC 9415. The Pennisetum purpureum Schum-derived bacillus megaterium has iron-producing carrier, azotification and IAA production characteristics, has an IAA yield of 10.57 microgrammes per milliliter, has a strong plant root colonization ability and good salt resistance, can grow in a culture medium containing 5% of NaCl (50g / L) and is an excellent plant growth promoting endophyte strain. Through infection of hybrid pennisetum with the Pennisetum purpureum Schum-derived bacillus megaterium, plant salt resistance is substantially improved, plant growth is promoted, and fresh weight, dry weight and height of the plant above the ground are substantially improved. The Pennisetum purpureum Schum-derived bacillus megaterium has important development and application prospects in study of hybrid pennisetum microbial fertilizer and promotion of growth and development of plants in saline marginal land.

Description

technical field [0001] The invention relates to a Bacillus megaterium derived from Elephant grass and its application, belonging to the technical field of applied microorganisms. Background technique [0002] In recent years, with the rise of the biomass energy industry, elephant grass ( Pennisetum purpureum schum.) and hybrid pennisetum have developed into a new type of energy plant due to their large biomass, strong regeneration ability, high content of cellulose and hemicellulose, and strong ecological adaptability. my country has large areas of marginal land suitable for energy such as coastal tidal flats, saline-alkali land, barren land, and barren land. The current biomass production potential exceeds 200 million tons of standard coal. Planting energy grasses such as elephant grass and hybrid pennisetum on marginal land suitable for energy, and developing biomass energy are in line with my country's energy development goal of "not competing for land with food, and...

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Problems solved by technology

However, at present, the endophytic bacteria that promote growth in plants mainly come from rice, corn, wheat, sugar cane, apple, citrus, highbush blueberry, mulberry, and apricot, and there are no reports of growth-promoting endophytes from Elephant grass ((1) Kloepper, J.W. 1994. Plant growth promoting bacteria (other systems). In: Okon, J. (Ed.), Azospirillum / Plant Association. CRC Press, Boca Raton, pp. 137–154.; (2) De Silva, A ., Petterson, K., Rothrock, C., Moore, J. Growth promotion of high bush blue berry by fungal and bacterial inoculants. 2000. Hort Science 35, 1228–1230.; (3) Sudhakar, P., Chattopadhyay, G.N., Gangwar, S.K., Ghosh, J.K. Effect of foliar application of Azotobacter, Azospirillum and Beijerinckia on leaf yield and quality of mulberry (Morus alba), 2000, J. Agric. Sci. 134, 227–234.; (4) Esitken, A., Karlidag, H., Ercisli, S., Sahin, F. Effects of foliar application of Bacillus substilis Osu-142 on the yield, growth and control of shot-hole disease (Coryneum blight) of apricot, 2002, Gartenbauwissenschaft 67, 139–142; (5) Esitken, A., Karlidag, H., Ercisli, S., Turan, M., Sahin, F. The effect of spraying a growth promoting bacteria on the yield, growth and nutrient element composition of leaves of apricot (Prunus armeniaca L. cv. Hacihaliloglu), 2003, Aus. J. Agric. Res. 54, 377–380.)

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