202 results about "Endophytic bacteria" patented technology

The disclosure provides materials and methods for conferring improved plant traits or benefits on plants. The materials can include a formulation comprising an exogenous endophytic bacterial population, which can be disposed on an exterior surface of a seed or seedling, typically in an amount effective to colonize the plant. The formulations can include at least one member selected from the group consisting of an agriculturally compatible carrier, a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, and a nutrient.

The disclosure provides materials and methods for conferring improved plant traits or benefits on plants. The materials can include a formulation comprising an exogenous endophytic bacterial population, which can be disposed on an exterior surface of a seed or seedling, typically in an amount effective to colonize the plant. The formulations can include at least one member selected from the group consisting of an agriculturally compatible carrier, a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, and a nutrient.

The subject invention concerns materials and methods for providing or enhancing nitrogen fixation in plants. The invention provides for the use of nitrogen fixing bacteria that are isolated from nitrogen efficient plants. Plants for which enhanced nitrogen fixation is desired are inoculated with an effective amount of nitrogen firing bacteria of the invention. In an exemplified embodiment, the bacteria is Klebsiella Kp342. The subject invention also concerns means to increase the number of free-living nitrogen-fixing bacteria in plants. Mutants of beneficial endophytic bacteria that are resistant to plant defense responses can be used to colonize a plant in numbers higher than a wild type or a non-mutated bacteria can colonize a plant. The higher number of bacteria colonizing the plant provide for more nitrogen fixation for the plant. The subject invention concerns methods for producing non-leguminous plants that are capable e of utilizing atmospheric nitrogen by colonization with a nitrogen fixing endophyic bacteria that is resistant to plant defense responses. The subject invention also concerns the plants produced by the subject method. The subject invention also concerns methods for producing the mutant endophytic bacteria. The subject invention also concerns the mutant endophytic bacteria produced using the subject methods.

The invention relates to an endophytic bacterial agent for promoting cucumber growth and preventing fusarium wilt of cucumber, which is prepared by bacillus subtilis. The technical scheme adopted by the endophytic bacterial agent is as follows: mother solution is prepared by 0.7 to 2.0% of peptone, 0.3 to 1.0% of yeast extract, 0.5 to 1.0% of NaCl and the rest percent of water; then, the mother solution with the pH value of 5 to 9 is cultivated for 24 to 72 hours in oscillating and fermenting manners at the speed of 120 to 200r / min under the fermentation temperature of 20 to 32 DEG C according to the liquid volume of 25 to 55% and the inoculum size of 2 to 10%.

The invention relates to methods for promoting fixed nitrogen from atmospheric nitrogen, and related products. Endophytic bacteria having an exogenous nif cluster promote fixed nitrogen for cereal plants.

The invention discloses Bacillus velezensis 05-1205 as well as an acquisition method and application thereof, and belongs to the technical field of microbial application. The Bacillus velezensis 05-1205 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the Bacillus velezensis 05-1205 is CCTCC No: M2020568. The acquisition method comprises processes of separating, screening and purifying, firstly, endophytic bacteria are seperated from healthy tobacco plants, endophytic bacteria antagonizing rhizopus oryzae are screened by taking the tobacco mildew pathogenic bacteria rhizopus oryzae in a baking period as an indicator, and finally, purifying is conducted on a culture medium plate to obtain the Bacillus velezensis 05-1205. The application ofthe bacterial strain comprises application in tobacco mildew control, the bacterial strain Bacillus velezensis 05-1205 can achieve effective control over tobacco mildew in the baking period, and therelative control effect is 70% or above. Secondly, the Bacillus velezensis 05-1205 is wide in antibacterial spectrum, and has a very good antibacterial effect on tobacco bacterial wilt bacteria, botrytis cinerea, sclerotinia sclerotiorum, target leaf spot bacteria, root rot bacteria and alternaria alternata besides pathogenic bacteria of tobacco mildew in the baking period.

The invention relates to a method for producing a biological pesticide by using endophytic bacillus subtilis, and belongs to the field of biological pesticide preparation. According to the method, the strain of the endophytic bacillus subtilis is subjected to shake cultivation for 24 hours in a seed culture solution containing 0.3% of beef extract, 1% of peptone, and 0.5% of NaCl at a temperature of 32 DEG C and a rotation speed of 170 r / min, wherein the resulting seed culture solution is adopted as the production bacteria seed; the resulting seed culture solution is inoculated in a fermentation culture medium comprising soybean cake powder, soluble starch, peptone and NaCl to carry out fermentation culture for 72 hours at a fermentation temperature of 30-34 DEG C under a condition of the pH value of 5-9, wherein the inoculation amount of the seed culture solution is 6% of the fermentation culture medium; the fermentation culture medium with the bacterial concentration more than 0.5*10<9> cfu / mL is subject to spray drying to prepare into the wettable powder with the bacterial content more than 2.0*10<9> cfu / g. With the method of the present invention, the characteristics of the endophytic bacillus subtilis are adopted, such that the significant prevention and treatment effect of the wheat sharp eyespot is provided, wherein the characteristics of the endophytic bacillus subtilis are that: the endophytic bacillus subtilis can colonize on the wheat rhizosphere and in the body, and the endophytic bacteria can produce biological surface active element (surfactin) antibacterial matters.

The invention discloses bacillus velezensis Z002 as well as an acquisition method and application thereof. The bacillus velezensis Z002 is collected in China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC No: M 2020567. The acquisition method comprises separation, screening and purification processes. Endophytic bacteria are firstly separated from tobacco plant tissue and subjected to dark culture, the endophytic bacteria antagonizing the tobacco mildew pathogen rhizopus oryzae are screened out, and finally purification is carried out to obtain the bacillus velezensis Z002. The application of the bacillus velezensis Z002 comprises fermentation, inoculation and modulation processes. Firstly, strains are cultured to obtain a fermentation inoculant, the fermentation inoculant is sprayed to tobacco petioles through a sprayer, tobacco leaves are put into a curing barn, and the tobacco leaves are cured according to a normal curing process. The strain bacillusvelezensis Z002 can effectively control tobacco mildew in the curing period, and the relative control effect is 80% or above. Secondly, the bacillus velezensis Z002 also has very good bacteriostatic effects on Ralstonia pseudosolanacearum, Phylophthora nicotianae, Botrytis cinerea, Sclerotinia sclerotiorum, Thanatephorus cucumeris, Fusarium oxysporum, Alternaria alternata and the like. The bacillus velezensis Z002 has good control effect and higher application and popularization value.