202 results about "Endophytic bacteria" patented technology

The present invention provides a rice plant that shows enhanced growth and increased seed production, which also enables reduction of the use of chemical fertilizers.In the present invention, a nitrogen-fixing endophytic bacterium is isolated from bacteria symbiotically inhabiting natural plants, the isolated endophytic bacterium is artificially proliferated and then artificially inoculated into rice plants, and thus, the nitrogen-fixing endophytic bacteria are allowed to infect to rice plants.

The objective of the present invention is to confer pest resistance to plants of Poaceae without using any chemically synthesized pesticides. The pest resistance can be conferred to plants of Poaceae by isolating from a natural plant an endophytic bacterium capable of expressing pest resistance, artificially culturing the endophytic bacterium, and introducing the bacteria to a Poaceae plant of interest.

The disclosure provides materials and methods for conferring improved plant traits or benefits on plants. The materials can include a formulation comprising an exogenous endophytic bacterial population, which can be disposed on an exterior surface of a seed or seedling, typically in an amount effective to colonize the plant. The formulations can include at least one member selected from the group consisting of an agriculturally compatible carrier, a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, and a nutrient.

The invention discloses a separation and selection method of antibiotic antitubercular endophyte, which comprises the steps as follows, the preparation of antibiotic antitubercular plant tissue, the surface sterilization and the sterility test, the separation and the purification of the endophyte, the ferment of endogenetic fungi, the ferment of endogenetic bacterium, the process of the zymotic fluid, the process of the thalli, the selection of the effective antibiotic bacterial strains, the selection of the effective antitubercular bacterial strains and the preservation of the strains. The separation and selection method of antibiotic antitubercular endophyte ensures the realization of obtaining the effective antibiotic antitubercular matter from microorganisms, thanks to separation and selection method, a batch of effective bacterial strains are obtained.

The subject invention concerns materials and methods for providing or enhancing nitrogen fixation in plants. The invention provides for the use of nitrogen fixing bacteria that are isolated from nitrogen efficient plants. Plants for which enhanced nitrogen fixation is desired are inoculated with an effective amount of nitrogen firing bacteria of the invention. In an exemplified embodiment, the bacteria is Klebsiella Kp342. The subject invention also concerns means to increase the number of free-living nitrogen-fixing bacteria in plants. Mutants of beneficial endophytic bacteria that are resistant to plant defense responses can be used to colonize a plant in numbers higher than a wild type or a non-mutated bacteria can colonize a plant. The higher number of bacteria colonizing the plant provide for more nitrogen fixation for the plant. The subject invention concerns methods for producing non-leguminous plants that are capable e of utilizing atmospheric nitrogen by colonization with a nitrogen fixing endophyic bacteria that is resistant to plant defense responses. The subject invention also concerns the plants produced by the subject method. The subject invention also concerns methods for producing the mutant endophytic bacteria. The subject invention also concerns the mutant endophytic bacteria produced using the subject methods.

The invention discloses a complex endophytic bacterial inoculant and an application thereof in phytoremediation of heavy metal contaminated soil. The complex inoculant is composed of acinetobactercalcoaceticus, pseudomonas fluorescens, bacillusmegaterium, stenotrophomonasmaltophilia, bacillus pumilus and pseudomonas putida. The complex inoculant disclosed by the invention, when applied to heavy metal pollution remediation, integrates advantages of various endophytic bacteria and overcomes disadvantages of phytoremediation; and the complex inoculant can promote the growth of sedum alfredii hance, enhance the absorbing capacity of the sedum alfredii hance to heavy metals and improve the efficiency of phytoremediation.

The invention discloses a plant endophyte 16S rRNA gene amplification method and application. The amplification method includes the following steps of plant pretreating; plant-sample total DNA extracting; sample 16S rRNA gene amplifying, wherein sample 16S rRNA gene amplifying comprises 16S rRNA gene total-length emulsion PCR amplifying and 16S rRNA gene hypervariable-region / conserved-region amplifying sub-amplifying; high-throughput sequencing and biological information analyzing based on a Illumina platform, wherein high-throughput sequencing and biological information analyzing based on the Illumina platform comprises plant endophyte 16S rRNA gene amplicon purifying and recycling, amplicon sequencing library establishing, Illumina HiSeq sequencing and sequencing data bioinformatics analysis. The gene amplification method is used for high-throughput-sequencing plant disease detection and phytophagous animal enteric microorganism detection. Sequencing analysis of plant endophytic bacteria is carried out with the high-throughput sequencing technology, the data size is larger, the detection result is more complete, pollution of plant hosts is reduced to the maximum degree, the result multiformity is higher, and the cost is low.

The invention relates to a method for using soybean root nodule and endotrophic mycorrhiza to prevent soybean root disease. Wherein, the inventive bacteria seed has been stored in national microbe storage manager center, whose storage number is sinorhizobium sp; the seed L396 storage number is CGMCC No. 1851; the bacillus subtilis seed Snb2 storage number is CGMCC No.1850. And the invention uses general liquid to ferment and cultivate, uses itself or metabolite as effective active component; mixing the ferment solutions at different ratios, with effective restrain function on soybean cyst nematization and root pathogenic fungi; the density ratio between Snb2 and L392 is 8:3, and the dead ratio of young bacteria can reach 98.92%, and the relative restrain rate on cyst cultivation can reach 99.66%; the invention prepares the metabolite into water agent, to reach 78.6% prevent rate on the soybean cyst nematization. The inventive nodule bacteria can fix nitrogen and accelerate growth.

The invention discloses application of bacillus amyloliquefaciens in preventing and controlling fungal disease of plants. The application has the advantages that found by study, the bacillus amyloliquefaciens, especially bacillus amyloliquefaciens BSY3 separated and screened from African marigold by an inventor, has obvious antagonistic function on phellinus noxius, pyricularia cannaecola hashioka, sclerotinia sclerotiorum, colletotrichum sp., pestalotiopsissp, fusarium oxysporum f.sp.cubense and the like, and has obvious biological control function and potential application value on preventing and controlling of the fungi diseases due to pathogenic bacteria; the bacillus amyloliquefaciens comes from endophytic bacteria in plants, so that compared with the traditional chemical agent preventing and control method, the safer biological control effect on the fungi diseases is realized; because the bacillus amyloliquefaciens stays in the plant for a long time, the survival relationship with harmonious coexistence and stable development is established with the plant; compared with the chemical agent, the biocontrol function can be more effectively and stably realized.

The invention relates to Pseudomonas aeruginosa FJAT-346 which is obtained by separating and screening an endosymbiotic bacterium in banana plants cultivated in fields with serious banana blight and has biologic al control action. The stain is Pseudomonas aeruginosa FJAT-346 and is used for preparing a fermentation liquid capable of controlling banana blight. The invention has the advantages that the endosymbiotic bacterium FJAT-346 is obtained by separating from the endosymbiotic bacterium in the banana plants, the fermentation liquid being capable of controlling banana blight and being prepared by using the stain has excellent inhibition action on banana blight bacteria, and the preparation process of the fermentation liquid is simple; and what's more important, the fermentation liquid is completely harmless and has good production application prospect.

The invention relates to a wall-breaking technique of ganoderma lucidum spore, in particular to a biologic method for carrying out wall breaking on the ganoderma lucidum spore. The method comprises the following steps of: separating and purifying ganoderma endophytic bacteria from ganoderma; sieving strains; preparing enzyme liquid; carrying out enzymolysis on the ganoderma lucidum spore; and preparing wall-breaking ganoderma lucidum spore powder. The invention utilizes the self symbiotic bacteria of the ganoderma to carry out fermentation on the spore and a fruiting body of the ganoderma, the effective ingredients of the ganoderma more easily escape from cells, a human body can successfully absorb and digest the effective ingredients, and the effective ingredients can be fully utilized. The difficulty that biologic residues caused by introducing foreign bacteria are hard to remove can be eliminated, and the safety of a product is ensured.

The invention relates to a Paenibacillus polymyxa Jaas ed2 with the functions of disease prevention and growth promotion to crops and application thereof, and belongs to the technical field of intensive agriculture production. The bacterial strain Jaas ed2 adopted by the invention has been preserved at the General Microbiological Culture Collection Center of China Microbiological Culture Collection Administration Committee on Sep. 18th, 2012 with the preservation number of CGMCC No.6580. The bacterial strain has the antagonistic activity to verticillium lecanii of eggplant, oxysporum of watermelon and other various pathogenic fungi, can enter plants of eggplants and watermelons in case of lower concentration of bacterium for colonization and conduction in the crops, can be used for promoting growth and preventing diseases of crops, and particularly has excellent control efficiency to soil-borne vascular bundle diseases, is low in production cost, simple to operate, facilitates ecological environment protection for farmlands, and reduces pesticide residue when used as a preparation, thereby meeting requirements for production of nuisance free agricultural products in China.

The invention discloses endophytic azotobacter of wheat producing ACC (1-aminocyclopropane-1-carboxylate) deaminase and application thereof. The endophytic azotobacter of wheat is pseudomonas sp. GDXM114, and is collected in the China General Microbiological Culture Collection Center (CGMCC) with the collection number of CGMCC No.5040. The pseudomonas sp. GDXM114 with the collection number of CGMCC No.5040 has the advantages of higher nitrogenase activity, ACC deaminase activity and wide application prospect in producing nitrogenase microorganism inoculants and microbial organic fertilizers.

The invention relates to a Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 and an application, which belongs to the biology technical field. The Pantoea agglomerans1-7 is preserved in the China General Microbiological Culture Collection Center on May 28th, 2012 with the preservation number of CGMCC No.6160. The Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 is used for preparing the preparations used for preventing and treating bacterial soft rot of konjak and clubroot disease on cruciferous vegetable club root in fields. The Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 has the advantages of high efficiency, no toxicity, safety and no residue, the bacterial strain1-7 has the characteristics of good disease control, better growth promotion effect, better comprehensive characters and easy industrial production, and the Pantoea agglomerans1-7 can be applied to preparation of preparations for controlling cruciferous vegetable club root in fields.

The invention relates to an endophytic bacterial agent for promoting cucumber growth and preventing fusarium wilt of cucumber, which is prepared by bacillus subtilis. The technical scheme adopted by the endophytic bacterial agent is as follows: mother solution is prepared by 0.7 to 2.0% of peptone, 0.3 to 1.0% of yeast extract, 0.5 to 1.0% of NaCl and the rest percent of water; then, the mother solution with the pH value of 5 to 9 is cultivated for 24 to 72 hours in oscillating and fermenting manners at the speed of 120 to 200r / min under the fermentation temperature of 20 to 32 DEG C according to the liquid volume of 25 to 55% and the inoculum size of 2 to 10%.

The invention relates to the technical field of microbes. The eucommia ulmoides endogenous bacteria are separated from eucommia ulmoides living plants by adopting an endogenous bacteria microbe separation technology. Through identification by microbial taxonomy, strain is named Bacillus pumilus DZBT01 and is collected in China General Microbiological Culture Collection Center, the collection number is January 30, 2015, and the collection register number is CGMCC NO.10470. The strain disclosed by the invention has the characteristics that the strain can achieve an obvious effect of inhibiting plant pathogenic fungi. The invention discloses an application of the Bacillus pumilus DZBT01 in control of wheat sharp eyespot and an application of Bacillus pumilus in promotion of wheat growth. The Bacillus pumilus DZBT01 disclosed by the invention refers to eucommia ulmoides plant endogenous bacteria, is simple in culture conditions and easy to preserve and refers to an agriculturally important microbial resource.

The invention relates to methods for promoting fixed nitrogen from atmospheric nitrogen, and related products. Endophytic bacteria having an exogenous nif cluster promote fixed nitrogen for cereal plants.

The invention discloses Bacillus velezensis 05-1205 as well as an acquisition method and application thereof, and belongs to the technical field of microbial application. The Bacillus velezensis 05-1205 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the Bacillus velezensis 05-1205 is CCTCC No: M2020568. The acquisition method comprises processes of separating, screening and purifying, firstly, endophytic bacteria are seperated from healthy tobacco plants, endophytic bacteria antagonizing rhizopus oryzae are screened by taking the tobacco mildew pathogenic bacteria rhizopus oryzae in a baking period as an indicator, and finally, purifying is conducted on a culture medium plate to obtain the Bacillus velezensis 05-1205. The application ofthe bacterial strain comprises application in tobacco mildew control, the bacterial strain Bacillus velezensis 05-1205 can achieve effective control over tobacco mildew in the baking period, and therelative control effect is 70% or above. Secondly, the Bacillus velezensis 05-1205 is wide in antibacterial spectrum, and has a very good antibacterial effect on tobacco bacterial wilt bacteria, botrytis cinerea, sclerotinia sclerotiorum, target leaf spot bacteria, root rot bacteria and alternaria alternata besides pathogenic bacteria of tobacco mildew in the baking period.

The invention discloses a banana endophytic bacillus velezensis strain and an application thereof. The name of the bacillus velezensis strain is bacillus velezensis BS (Bacillus velezensis). The preservation number is GDMCC No: 60930, and the strain is preserved in Guangdong Province Microbial Culture Collection Center of 59 # building of 100 # courtyard of Xianlie Middle Road of Guangzhou City onDecember 11, 2019. The strain disclosed by the invention belongs to banana endophytic bacteria, has no pathogenicity and has a growth promoting effect on bananas. The bacterial strain and the biological agent thereof have a remarkable inhibition effect on banana wilt pathogen FOC4, the banana wilt symptom is obviously relieved, and the biological agent is environment-friendly and non-toxic in source and has small influence on the ecological environment; the strain has different degrees of inhibition effects on plant pathogenic fungi of different genera; the culture conditions are simple, a propagation speed is fast, thalli and a bacterial liquid can serve as main bodies for prevention and control, storage and transportation are easy, and good development and application prospects are achieved.

The invention discloses a plant-growth-promoting endophytic bacterium having a polycyclic aromatic hydrocarbons degrading function and application thereof. The plant-growth-promoting endophytic bacterium having the polycyclic aromatic hydrocarbons degrading function, namely Massilia bacterium Pn2 is preserved in China General Microbiological Culture Collection Center and has the preservation number of CGMCC 1.12927. By virtue of a microbial agent prepared by the strain, the degradation rate of phenanthrene of which the initial concentration is 150mg.L<-1> can reach up to above 95% within 3 days under the condition of laboratory shake-flask culture and meanwhile, naphthalene, acenaphthene, anthracene and pyrene can be degraded in different degrees by virtue of the bacterium, the microbial agent is colonized on the surface of a plant root by virtue of a root dipping treatment and then the plant is planted in a nutrient solution contaminated by virtue of phenanthrene so that the content of phenanthrene within a plant body can be reduced by above 50% within short time, the risk of phenanthrene contamination of the plant body is obviously reduced and the endophytic bacterium has a significant plant growth promoting effect.

The disclosure provides materials and methods for conferring improved plant traits or benefits on plants. The materials can include a formulation comprising an exogenous endophytic bacterial population, which can be disposed on an exterior surface of a seed or seedling, typically in an amount effective to colonize the plant. The formulations can include at least one member selected from the group consisting of an agriculturally compatible carrier, a tackifier, a microbial stabilizer, a fungicide, an antibacterial agent, an herbicide, a nematicide, an insecticide, a plant growth regulator, a rodenticide, and a nutrient.

The invention belongs to the technical field of agriculture and environmental pollution treatment, and relates to a plant endogenetic bacterium with heavy metal tolerance and an application thereof. The plant endogenetic bacterium with heavy metal tolerance is preserved in CCTCC (China Center for Type Culture Collection), the preservation date is on July 26th in 2011, the preservation number of the strain is CCTCC No.M2011251. The bacterial strain liquid preparation contains more than 1 billion / milliliter of effective viable count, the solid preparation contains more than 0.2 billion / gram of effective viable count. The strain has good activation effect on the precipitation state copper in a solution, the activation rate is more than 73%. That the plant is implanted and the bioremediation preparation is inoculated in humid soil containing heavy metals promote the absorption and the extraction of the plant to the heavy metal copper and improve the extraction and recovery efficiency.

The invention discloses Bacillus velezensis ZJU-C612-2, and a preservation number thereof is CCTCC NO: M 2018312. The Bacillus velezensis ZJU-C612-2 is capable of inhibiting growth of dendrobium officinale colletotrichum orbiculare, saffron corm valsa ceratosperma, walnut twig wilt pathogenic bacteria, sclerotinia sclerotiorum, cotton rhizoctonia solani pathogenic bacteria, and didymella bryoniae,and promoting growth of a dendrobium officinale protocorm and a tissue culture seedling.

The invention discloses a method of analyzing rice phyllospheric endophytic bacteria community structure, comprising the steps of a) extracting total DNA of a plant and its phyllospheric endophytic bacteria; b) using the total DNA as a template, using a primer pair 1 (sequence 1 and sequence 2) to perform first PCR (polymerase chain reaction) amplification to obtain PCR product 1; using the PCR product 1 as a template, and using primer pair 2 (sequence 3 and sequence 4) to perform second PCR amplification to obtain PCR product 2; (c) sequencing the PCR product 2, and identifying species according to sequencing results so as to analyze the rice phyllospheric endophytic bacteria community structure. The gate to study non-culture processes of plant phyllospheric endophytic bacteria is opened, subsequent study on this field is made possible, and references are provided for the whole field of study on plant phyllospheric endophytic bacteria.

The invention discloses an endophytic bacillus flexus having salt resistance PE4941, which is preserved in Guangdong microbe bacterial preservation administration center with a preservation number being GDMCC 60176 on May 10th, 2017; and provides an application of the endophytic bacillus flexus for promoting vegetation growth under salt stress. According to the invention, the separated and screened limonium sinense endophytic bacteria bacillus flexus PE4941 has a plurality of growth-promotion characteristics such as nitrogen fixation and iron carrier generation, the endophytic bacteria can tolerate 8% of NaCl, limonium sinense seedlings can be inoculated by pouring root, a salt damage symptom under salt stress is obviously alleviated, biomass is increased, growth effect is promoted, salt resistance of the plants is increased, and the usage of a chemical fertilizer is reduced.

The invention relates to a method of preparing a bioactive substance with co-cultivation of a plant-based raw material and endophytic bacteria. The method includes the steps of: 1) pretreatment to the plant-based raw material: preparing sterile-cultured propagules, such as living plant cells and tissues, or drying and / or performing pulverization to the plant-based raw material, and performing sterilization pretreatment; 2) activation to the endophytic bacteria: inoculating the endophytic bacteria on a microbial culture medium to perform pre-cultivation for 1-25 days to produce activated endophytic bacteria; 3) co-cultivation of the bio-material and the endophytic bacteria: in a co-cultivation device, inoculating the activated endophytic bacteria in the step 2) and adding a solid or liquid culture medium, which contains the plant-based raw material and is prepared in the step 1), to perform mixed cultivation for 4-90 days to obtain a co-cultivation product; and 4) extraction and preparation of the bioactive substance. Through the co-cultivation of the plant-based raw material and the endophytic bacteria, the bioactive substance is prepared. The method is simple and controllable, achieves stable expression of the product and is short in production period.

The invention relates to a method for producing a biological pesticide by using endophytic bacillus subtilis, and belongs to the field of biological pesticide preparation. According to the method, the strain of the endophytic bacillus subtilis is subjected to shake cultivation for 24 hours in a seed culture solution containing 0.3% of beef extract, 1% of peptone, and 0.5% of NaCl at a temperature of 32 DEG C and a rotation speed of 170 r / min, wherein the resulting seed culture solution is adopted as the production bacteria seed; the resulting seed culture solution is inoculated in a fermentation culture medium comprising soybean cake powder, soluble starch, peptone and NaCl to carry out fermentation culture for 72 hours at a fermentation temperature of 30-34 DEG C under a condition of the pH value of 5-9, wherein the inoculation amount of the seed culture solution is 6% of the fermentation culture medium; the fermentation culture medium with the bacterial concentration more than 0.5*10<9> cfu / mL is subject to spray drying to prepare into the wettable powder with the bacterial content more than 2.0*10<9> cfu / g. With the method of the present invention, the characteristics of the endophytic bacillus subtilis are adopted, such that the significant prevention and treatment effect of the wheat sharp eyespot is provided, wherein the characteristics of the endophytic bacillus subtilis are that: the endophytic bacillus subtilis can colonize on the wheat rhizosphere and in the body, and the endophytic bacteria can produce biological surface active element (surfactin) antibacterial matters.

The invention discloses bacillus velezensis Z002 as well as an acquisition method and application thereof. The bacillus velezensis Z002 is collected in China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC No: M 2020567. The acquisition method comprises separation, screening and purification processes. Endophytic bacteria are firstly separated from tobacco plant tissue and subjected to dark culture, the endophytic bacteria antagonizing the tobacco mildew pathogen rhizopus oryzae are screened out, and finally purification is carried out to obtain the bacillus velezensis Z002. The application of the bacillus velezensis Z002 comprises fermentation, inoculation and modulation processes. Firstly, strains are cultured to obtain a fermentation inoculant, the fermentation inoculant is sprayed to tobacco petioles through a sprayer, tobacco leaves are put into a curing barn, and the tobacco leaves are cured according to a normal curing process. The strain bacillusvelezensis Z002 can effectively control tobacco mildew in the curing period, and the relative control effect is 80% or above. Secondly, the bacillus velezensis Z002 also has very good bacteriostatic effects on Ralstonia pseudosolanacearum, Phylophthora nicotianae, Botrytis cinerea, Sclerotinia sclerotiorum, Thanatephorus cucumeris, Fusarium oxysporum, Alternaria alternata and the like. The bacillus velezensis Z002 has good control effect and higher application and popularization value.

The invention discloses a polygonatum cyrtonema endophyte strain ZJU-C612-1, i.e. Bacillus velezensis ZJU-C612-1 with a preservation number of CCTCC NO: M 2018311. The invention also discloses the uses of the strain in inhibiting the growth of Colletotrichum gloeosporioides, Fusarium oxysporum, C. truncatum, F. graminearum, F. solami, Rhizoctonia. solani and F. oxyporum f. sp. Niveum and promotingthe growth of polygonatum kingianum tissue culture seedlings and polygonatum cyrtonema tubers.