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Plant endogenetic bacterium with heavy metal tolerance and application thereof

A technology of endogenous bacteria and heavy metals in plants, applied in the fields of agriculture and environmental pollution control, can solve the problems of long cycle, restricting the development of phytoremediation technology, and slow growth, so as to improve the efficiency of remediation, improve the resistance of plants, and promote the growth of plants Effect

Inactive Publication Date: 2012-03-28
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most hyperaccumulative plants grow slowly, have a long cycle, have small biomass, and are selective to metals, which restrict the development of phytoremediation technology.

Method used

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  • Plant endogenetic bacterium with heavy metal tolerance and application thereof
  • Plant endogenetic bacterium with heavy metal tolerance and application thereof
  • Plant endogenetic bacterium with heavy metal tolerance and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The heavy metal-resistant plant endophytic bacteria Y1-3-9 bacterial strain of the present invention is isolated and purified from the leaves of Mosla chinensis, and the isolation and identification method is as follows:

[0027] First clean the surface of the leaves of A. chinensis with tap water, then wash them once with sterile water, then soak them in 75% ethanol for 5 minutes, rinse them with sterile water, then immerse them in 2.5% sodium hypochlorite solution for 5 minutes, and rinse them several times with sterile water. Put the surface-sterilized leaves in a mortar and grind until homogenized, take 0.1ml of the homogenate and spread it on an LB plate (10g of peptone, 5g of yeast extract, 10g of NaCl, 1000mL of water, 20g of agar, pH 7.0), and incubate at 28°C for 3 days , Pick a single colony, streak it on an LB plate and save it after purification. The strains were inoculated in LB culture medium, cultured on a shaker at 30°C for 18 hours, 1.0 mL of the bacter...

Embodiment 2

[0029] Streak inoculation of Y1-3-9 (CCTCC NO: M 2011251) in LB medium containing different concentrations of heavy metals, culture at 30°C for 3 days, and observe its growth and growth conditions. The results are shown in Table 1. Y1-3-9 strain can tolerate many heavy metals (the minimum inhibitory concentrations of Cu, Pb, Cd, Ni are 3.1, 4.8, 4.5, 1.7mM, respectively).

[0030] Table 1 The growth of bacterial strains on LB medium containing different concentrations of heavy metals

[0031]

[0032] In the above table, "+" means growth, and "-" means no growth.

Embodiment 3

[0034] Inoculate Y1-3-9 (CCTCC NO: M 2011251) in LB culture medium and shake it for 20 hours, collect the bacteria by centrifugation at 4°C, and use SM culture medium (glucose 1.0g, sucrose 1.0g, sodium citrate 1.0g , malic acid 1.0g, mannitol 1.0g, sodium acetate 1.0g, KH 2 PO 4 0.4g, K 2 HPO 4 2.0g, MgSO 4 0.2g, CaCl 2 0.1g, CuSO 4 1mg, NiSO 4 1mg, ZnSO 4 5mg, FeSO 4 5mg, MnSO 4 3 mg, CoSO 4 1 mg, Na 2 MoO 4 1 mg, H 3 BO 3 2mg, Biotin 2 (VB 6 ) 10mg, thiamine (VB 1 ) 2mg, cyanocobalamin (VB 12 )0.1mg, pantothenic acid (VB 3 ) 5mg, folic acid 2mg, riboflavin 5mg, niacin 5mg, distilled water 1000mL, pH 6.4. Vitamins were added after filter sterilisation. ) was washed and centrifuged twice, the bacteria were suspended in SM culture medium, and inoculated in SMA culture medium according to 5% inoculum [Add filter-sterilized ACC (1-aminocyclopropane-1-carboxylic aciddeaminase) to the sterile SM medium , so that the final concentration is 3mM], cultured at 28°C...

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Abstract

The invention belongs to the technical field of agriculture and environmental pollution treatment, and relates to a plant endogenetic bacterium with heavy metal tolerance and an application thereof. The plant endogenetic bacterium with heavy metal tolerance is preserved in CCTCC (China Center for Type Culture Collection), the preservation date is on July 26th in 2011, the preservation number of the strain is CCTCC No.M2011251. The bacterial strain liquid preparation contains more than 1 billion / milliliter of effective viable count, the solid preparation contains more than 0.2 billion / gram of effective viable count. The strain has good activation effect on the precipitation state copper in a solution, the activation rate is more than 73%. That the plant is implanted and the bioremediation preparation is inoculated in humid soil containing heavy metals promote the absorption and the extraction of the plant to the heavy metal copper and improve the extraction and recovery efficiency.

Description

technical field [0001] The invention belongs to the technical field of agriculture and environmental pollution control, and relates to a heavy metal-resistant plant endophytic bacterium and its application. Background technique [0002] Research on soil heavy metal pollution and its remediation is a major issue related to the sustainable utilization of soil resources. Bioremediation of contaminated soil using plants focuses on pollution control and landscaping, ecological restoration and reconstruction, and sustainable development, and has attracted extensive attention and attention from domestic and foreign governments, universities, research institutions, and commercial enterprises. Phytoremediation techniques include phytostabilization, phytovolatilization and phytoextraction. However, the development of phytoremediation technology relies heavily on the screening and growth of hyperaccumulative plants, and the treatment effect depends on the extraction and fixation of he...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/14B09C1/10C12R1/38
Inventor 盛下放何琳燕张艳峰黄智张文辉
Owner NANJING AGRICULTURAL UNIVERSITY
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