The invention relates to the field of skin care products, in particular to a mineral spring rejuvenating lotion and a preparation method thereof. The mineral spring rejuvenating lotion comprises water, 1,3-propylene glycol, glycerinum, trehalose, nicotinamide, cyanocobalamin, phenoxyethanol, allantoin, sodium hyaluronate, 3-o-ethyl ascorbic acid, panthenol and hydrolyzed collagen. Through a synergistic effect of multiple vitamins of the nicotinamide, 3-o-ethyl ascorbic acid, panthenol, cyanocobalamin and tocopherol, in cooperation with raw materials such as hydrolyzed collagen, trehalose and sodium hyaluronate, the mineral spring rejuvenating lotion is prepared, skin pores are purified, convenience is provided for the skin to absorb nutrient components, the water flocculation texture of the skin is restored, the translucent feeling and softness of the cuticle are maintained, and a mineral spring skin care lotion is supplemented, so that the skin feels active, moisturized and transparent; the vitamin B12 and sodium hyaluronate supplement each other, the skin metabolism is improved, absorption is promoted, the self-protection force of the substrate is effectively improved, and the conditions of dull skin and lowered elasticity are reduced; the spots are faded, the skin color is balanced, whiting and shining are achieved, the skin is transparent and free of speckles, and the skinis lustrous, transparent, bright and white.