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Vitamin B12 preparation method

A technology for B12 and vitamins, applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of difficult filtration, large sodium cyanide, etc., achieve high impurity removal rate, reduce production costs, and reduce investment Effect

Active Publication Date: 2015-11-04
河北美邦工程科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the aerobic fermentation process, a large amount of filter aid polyaluminum chloride needs to be added when the fermentation broth is filtered, otherwise the filtration will be very difficult, so the mycelium produced after filtration cannot be processed due to the filter aid
In addition, when the cyanocobalamin salt of VB12 is filtered, due to the addition of sodium cyanide, toxic cyanogenic hyphae will be produced after filtration, and this toxic mycelium will form toxic waste; for VB12 manufacturers with an annual output of 20,000 tons , the cation exchange resin column desorption filtrate (VB12 content is 1000μg / L) that produces every day reaches 20t, the amount that needs to add sodium cyanide is very large, the toxic cyanogen-containing hyphae that produces every day exceeds 3t, because the polluted waste water that this process exists emissions, limiting production applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Hydrolysis

[0031] Using glucose, corn steep liquor, molasses, and betaine as raw materials to ferment to obtain VB12 fermentation liquid, add water of 1 times the quality of the fermentation liquid to the fermentation liquid, heat to 93°C for hydrolysis, release intracellular VB12, and obtain a hydrolyzed liquid;

[0032] (2) Primary membrane filtration

[0033] The hydrolyzate obtained in step (1) is filtered with a ceramic membrane system with a filtration accuracy of 50nm to remove impurities such as protein and mycelium. The filtration pressure is 0.36-0.41Mpa and the temperature is 50°C. When descending, add a certain amount of water to the membrane system and continue to filter. When the VB12 content in the hydrolyzate is lower than 5μg / L, stop adding water, and after the filtration is completed, collect the membrane permeate once and the membrane concentrate once;

[0034] (3) Secondary membrane filtration

[0035] The primary membrane permeate obtained i...

Embodiment 2

[0050] (1) Hydrolysis

[0051] Fermentation of glucose, corn steep liquor, molasses, and betaine as raw materials to obtain VB12 fermentation liquid, adding water of 1 times the quality of the fermentation liquid to the fermentation liquid, heating to 100°C for hydrolysis, releasing the VB12 in the cells, and obtaining a hydrolyzed liquid;

[0052] (2) primary membrane filtration

[0053] The hydrolyzate obtained in step (1) is filtered with a ceramic membrane system with a filtration accuracy of 50nm to remove impurities such as protein and mycelium. The filtration pressure is 0.36-0.41Mpa and the temperature is 55°C. When descending, add a certain amount of water to the membrane system and continue to filter. When the VB12 content in the hydrolyzate is lower than 5μg / L, stop adding water, and after the filtration is completed, collect the membrane permeate once and the membrane concentrate once;

[0054] (3) Secondary membrane filtration

[0055] The primary membrane perme...

Embodiment 3

[0070] (1) Hydrolysis

[0071] Using glucose, corn steep liquor, molasses, and betaine as raw materials to ferment VB12 fermentation liquid, add water with 1 times the quality of fermentation liquid to the fermentation liquid, heat to 95°C for hydrolysis, release intracellular VB12, and obtain hydrolyzate;

[0072] (2) primary membrane filtration

[0073] The hydrolyzate obtained in step (1) is filtered with a metal membrane system with a filtration accuracy of 100nm to remove impurities such as protein and mycelium. The filtration pressure is 0.36-0.41Mpa and the temperature is 60°C. When descending, add a certain amount of water to the membrane system and continue to filter. When the VB12 content in the hydrolyzate is lower than 5μg / L, stop adding water, and after the filtration is completed, collect the membrane permeate once and the membrane concentrate once;

[0074] (3) Secondary membrane filtration

[0075] The primary membrane permeate obtained in step (2) is filtere...

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Abstract

The present invention discloses a vitamin B12 preparation method, VB12 fermentation broth is directly heated for hydrolysis to release intracellular VB12, cyanocobalamin salt can be obtained by secondary membrane filtration, drum centrifuging protein removal, protein adsorption, and other impurity removal processes, and nanofiltration concentration dehydration, and sodium cyanide convertion, and pure product cyanocobalamin, namely VB12 can be obtained by three-level resin adsorption column impurity removal and crystallization separation. According to the method, filtration-aid-free proteins accounting to more than 80% of the total protein can be obtained, protein and mycelium can be used for further processing to produce high value-added products, the dosage of sodium cyanide can be reduced by more than 90% compared that of a traditional process, production cost is reduced, due to high removal rate of proteins and other impurities in macromolecular solutions, no poisonous cyanide-containing mycelium is produced, the cation exchange resin adsorption step is eliminated, one- time investment is reduced, production time is shortened, productivity is improved, and VB12 total yield reaches 83%.

Description

technical field [0001] The invention relates to a preparation method of vitamin B12, in particular to a green and environment-friendly preparation method, belonging to the technical field of vitamin production. Background technique [0002] Vitamin B12, referred to as VB12, also known as cobalamin, is the only vitamin containing metal elements. Its crystal is red, so it is also called red vitamin. Vitamin B12 in nature is synthesized by microorganisms. It mainly exists in animal foods, such as viscera, liver, kidney, and pig heart. VB12 also exists in lean meat, fish, milk, and egg yolk. Plants do not contain VB12. The main physiological function of vitamin B12 is to participate in the manufacture of bone marrow red blood cells, prevent pernicious anemia, and prevent brain nerves from being damaged. my country is the main producer of VB12, domestic producers include North China Pharmaceutical Group, CSPC, Hebei Yufeng Group, Ningxia Duowei Pharmaceuticals, etc. The aerobi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H23/00C07H1/00
Inventor 刘东吕志张玉新邢建波张辰杰闫利军
Owner 河北美邦工程科技股份有限公司
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