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7965results about How to "Improve isolation" patented technology

Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells

Disclosed are methods and compositions for producing heterologous disulfide bond containing polypeptides in bacterial cells. In preferred embodiments the methods involve co-expression of a prokaryotic disulfide isomerase, such as DsbC or DsbG and a gene encoding a recombinant eukaryotic polypeptide. Exemplary polypeptides disclosed include tissue plasminogen activator.
Owner:GENENTECH INC +1

Determining presence and/or physiological motion of one or more subjects within a doppler radar system

InactiveUS20080074307A1Null be avoidReadily detected and isolatedDiagnostic recording/measuringSensorsGeneralized likelihood ratioRadar systems
Systems and methods for detecting presence and / or physiological motion of at least one subject using a Doppler radar system and determining a number of subjects within range are provided. In one example, the apparatus includes a receiver for receiving a transmitted source signal, the transmitted source signal modulated by at least one subject, logic for mixing the received transmitted signal and a local oscillator signal, and logic for performing a Generalized Likelihood Ratio Test (GLRT) on the mixed signal to determine a number of subjects modulating the signal. Additionally, the receiver may include a quadrature receiver operable for mixing the source signal and the received modulated source signal to generate in-phase (I) and quadrature (Q) data, whereby nulls in the signal are avoided.
Owner:UNIV OF HAWAII

Determining presence and/or physiological motion of one or more subjects with multiple receiver Doppler radar systems

Systems and methods for determining presence and / or physiological motion of at least one subject using a Doppler radar system are provided. In one example, the apparatus includes at least two inputs for receiving a transmitted signal (e.g., a continuous wave signal), the transmitted signal modulated during reflection from at least one subject, and logic (e.g., hardware, software, and / or firmware; including digital and / or analog logic) for determining physiological motion associated with the at least one subject (e.g., a heart rate and / or respiration rate of the subject). In one example the logic includes comparing (e.g., mixing) the received signal with the source signal. The apparatus may further comprise logic for quadrature detection of the received signals, and may include various blind source separation algorithms for detecting signals associated with separate subjects.
Owner:UNIV OF HAWAII

System and method for conditional tracing of computer programs

A software system is disclosed which facilitates the process of tracing the execution paths of a program, called the client. The tracing is performed without requiring modifications to the executable or source code files of the client. Trace data collected during the tracing operation is collected according to instructions in a trace options file. At run time, the tracing library attaches to the memory image of the client. The tracing library is configured to monitor execution of the client and to collect trace data, based on selections in the trace options file. Conditional tracing, through the use of triggers and actions taken in response to the triggers, allows the developer to control the tracing operation. The triggers can be conditional triggers in which the corresponding action is taken only if a conditional expression is satisfied. The system can trace multiple threads and multiple processes. The tracing system provides a remote mode and an online mode. In remote mode, the developer sends the trace control information (which can include triggers and corresponding actions) to a remote user site together with a small executable image called the agent that enables a remote customer, to generate a trace file that represents execution of the client application at the remote site. In online mode, the developer can generate trace options (including triggers and corresponding actions), run and trace the client, and display the trace results in near real-time on the display screen during execution of the client program.
Owner:BMC SOFTWARE ISRAEL LTD

System and method for conditional tracing of computer programs

A software system is disclosed which facilitates the process of tracing the execution paths of a program, called the client. The tracing is performed without requiring modifications to the executable or source code files of the client. Trace data collected during the tracing operation is collected according to instructions in a trace options file. At run time, the tracing library attaches to the memory image of the client. The tracing library is configured to monitor execution of the client and to collect trace data, based on selections in the trace options file. Conditional tracing, through the use of triggers and actions taken in response to the triggers, allows the developer to control the tracing operation. The triggers can be conditional triggers in which the corresponding action is taken only if a conditional expression is satisfied. The system can trace multiple threads and multiple processes. The tracing system provides a remote mode and an online mode. In remote mode, the developer sends the trace control information (which can include triggers and corresponding actions) to a remote user site together with a small executable image called the agent that enables a remote customer, to generate a trace file that represents execution of the client application at the remote site. In online mode, the developer can generate trace options (including triggers and corresponding actions), run and trace the client, and display the trace results in near real-time on the display screen during execution of the client program.
Owner:BMC SOFTWARE ISRAEL LTD

Probe Compound for Detecting and Isolating Enzymes and Means and Methods Using the Same

InactiveUS20120231972A1Easy to useHighly sensitive and accurate and reproducible and robust high-throughputOrganic compound preparationMicrobiological testing/measurementMetaboliteFluorescence
The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.
Owner:GESELLSCHAFT FUR BIOTECHNOLOGISCHE FORSCHUNG MBH GBF +1
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