688 results about "Riboflavin" patented technology

A system for bilateral or monocular photochemical cross-linking of corneal collagen employs selectable light in a selected wavelength band as the excitation source and riboflavin as the photosensitizer. The system has an illumination source which may have multi-spectral capability, light guides for delivery of light to the optical head for projection onto the corneal surface, selectable radiation patterns to accommodate individual corneal architecture, and red light phototherapy to limit apoptosis and accelerate healing time. Aiming beams provide alignment of the optical head to the patient cornea. A microprocessor-controlled rotary solenoid mechanical shutter provides discontinuous illumination for tissue reoxygenation, and devices and methods may be included for the in situ determination of oxygen utilization and the riboflavin content of the cornea.

Equi-dosed time-fractionated pulsed UVA is employed to irradiate a class of riboflavin / collagen mixture in the presence of copious oxygen to cause rapid crosslinking causing gelation of the riboflavin / collagen mixture in situ and to effect adhesion to underlying structure specifically ocular tissue such as scleral and corneal tissue. Irradiation according to an embodiment of the invention results in depletion of dissolved oxygen at a rate inversely related to irradiance and more particularly depletion of dissolved oxygen occurs rapidly during the process of generation / cross-linking of reactive oxygen species (ROS), specifically singlet oxygen, such that the pulsed fractionation of UVA radiation exposure increases cross-linking efficiency by allowing the re-diffusion of oxygen during pauses in exposure.

A system for accurately delivering bilateral simultaneous equi-dosed time-fractionated pulsed UVA to irradiate a class of riboflavin / collagen mixture in the presence of oxygen for treatment of ocular tissue such as scleral and corneal tissue. The system employs ocular trial frames for mounting on the face that are fitted with 1) a nozzle for introducing Riboflavin in solution to collagen on the surface of the ocular tissue, 2) a port for introducing oxygen-rich gas to the ocular tissue, and 3) a pair of optical collimator inserts mounted in the lens holders, wherein the collimator inserts have a mask in the optical path at an aperture on focal point to control the pattern of UVA radiation at the ocular target, the collimator inserts further having optical input ports coupled to a controlled source of UVA radiation that is operative in accordance with the related inventive method.

The present invention is directed to tooth whitening compositions that are capable of being activated by light energy, where the compositions include a safe and effective amount of riboflavin or a derivative of riboflavin, from about 0.1 to about 50 percent by weight of a bleaching agent and a water-soluble liquid phase; to systems for delivering the photoactivatable tooth whitening compositions to the surface of the teeth, and methods of whitening teeth that include the steps of exposing the photoactivatable whitening composition to light energy under conditions effective to photoactivate the tooth whitening composition and maintaining the composition in contact with the teeth under conditions effective to whiten teeth.

The invention relates to a human mesenchymal stem cell culture medium. According to the culture medium, the basal culture medium comprises the following components based on the final concentration: 1-2g / L of human serum albumin, 5-10mg / L of transferring, 2-8mg / L of fibronectin, 1-4mg / L of laminin, 50g / L of Fe(NO3)3.9H2O, 417g / L of FeSO4.7H2O, 1-3mu g / L of estradiol, 2-5mu g / L of testosterone, 1-3mu g / L of progesterone, 39.25-117.74 mu g / L of dexamethasone, 5-10mg / L of insulin, 376.36mg / L of riboflavin, 80.96-242.87mg / L of coenzyme A, 4.41-6.17mg / L of butanediamine, 1-2mg / L of taurine, 0.61-1.85mg / L of aminoethanol, 8.81-26.42mg / L of pyruvic acid, 3.78-7.56mu g / L of sodium selenate, 292.3-584.6mg / L of L-glutamine, 2-8mu g / L of vascular endothelial growth factor, 4-10mu g / L of epidermal growth factor, 4-10mu g / L of basic fibroblast growth factor, 1-5mu g / L of leukaemia inhibitory factor, 1-5mu g / L of insulin-like growth factor-I and 2-8mu g / L of stem cell factor. The culture medium does not contain the animal serum, the potential animal endogenous endotoxin or virus of the animal serum is eliminated, and the culture medium is conveniently applied to clinics.

The invention provides a pig feed capable of preventing piglets from diarrhea. The pig feed comprises a feed carrier, nano-montmorillonite, acidifier, a probiotic additive, propolis, fish meal, zinc sulfate, chromium nicotinate, methionine, lysine, choline chloride, salt, vitamin A, vitamin E, riboflavin and a traditional Chinese medicine feed additive. The use of the pig feed can decrease diarrhea rate, reduce the occurrence of diseases, promote the fast growth of piglets and increase economic benefits of farmers.

A method for administering an antioxidant composition to humans according to their age and sex is disclosed wherein the method comprises administering to said humans a daily dose of a multiple antioxidant micronutrient composition comprising vitamin A (palmitate), beta carotene (from natural d. salina), vitamin C (calcium ascorbate), vitamin D-3 (cholecalciferol), natural source vitamin E including both d-alpha tocopheryl and d-alpha tocopheryl acid succinate, thiamine mononitrate, riboflavin, niacinamide ascorbate, d-calcium pantothenate, pyridoxine hydrochloride, cyanocobalamin, folic acid (folacin), d-biotin, selenium (1-seleno methionine), chromium picolinate, zinc glycinate, calcium citrate, and magnesium citrate. For persons over the age of about 51, the composition preferably further comprises one or more of co-enzyme Q10, N-acetyl cysteine, and alpha lipoic acid. Preferably, also, vitamin D is added for women over the age of about 36.

The invention relates to a piece of surface enhanced raman detection test paper and the application thereof, which belong to the technical field of ultra-sensitive test analysis, in particular to the piece of surface enhanced raman detection test paper and the application of the paper to detection of biological or chemical probe molecules with raman signals. The surface enhanced raman detection test paper is obtained by covering a precious metal layer on the surface of paper with a natural fiber micro nano multi-grade structure through the physical vapor deposition or chemical plating technology, the paper can be filter paper, parchment paper, napkin, newspaper or printing paper and the like, the precious metal film is gold or silver and the like, and the thickness of the precious metal film is 5 nanometers to 90 nanometers. Combined action of size, period and roughness enables incident light to generate a local electric field on the surface of a multi-stage structure and enables the electric field to be enhanced, and the detection limit can reach 10-10mol / L. The test paper has the advantages of being good in flexibility, low in cost, free of environment pollution, capable of being prepared in batch and the like, thereby being capable of being used in detection of probe molecules such as rhodamine 6G, p-aminothiophenol, riboflavin or ethanol and the like.

Delivery systems and methods for delivering riboflavin (R / F) and UVA irradiation to the sclera are disclosed. The R / F is delivered and then activated with UVA irradiation through the use of LEDs or optical fibers, thereby causing cross-linking of the collagen tissue. Delivery systems include implantable structures which provide surfaces that conform to the sclera. The delivery systems include various types of structures for delivery of R / F onto the sclera surface. Additionally, the delivery systems include UVA sources which provide irradiation of R / F in sclera collagen tissue.

The invention provides a natural pigment composition. The natural pigment comprises an emulsifier and two or more than two pigments of gardenia yellow pigment, curcumin, carithamine, citroxanthin, xanthophyll, riboflavin, capsaicin, monascus red pigment, bixa orettana pigment and gardenia blue. Natural pigments can prepare natural pigment compositions in different color systems and different tones in arbitrary proportions, such as yellow, orange, red, green and purple. Meanwhile, the natural pigment composition contains the emulsifier, so that the composition can be more uniform and more stable. Therefore, in the natural pigment composition provided by the invention, combinations of different natural pigments can overcome the defect of single pigment, for example, the light resistance of natural yellow pigment combined by the riboflavin, the curcumin and the like with poor light resistance and the gardenia yellow pigment with more stable light resistance is better than that of the riboflavin or the curcumin, thus the dosage can be reduced, and the cost can be reduced.

Pharmaceutical compositions effective in alleviating or reducing the effects of fatigue and weakness associated with cancer and cancer chemotherapy are disclosed. The pharmaceutical compositions of the present invention comprise riboflavin, effectors of the urea cycle in free form or pharmacologically acceptable salts thereof, and amino acids selected from the groups of essential and non-essential amino acids, in free form or pharmaceutically acceptable salts thereof, suitably combined with appropriate carriers, diluents, or excipients. Also disclosed are methods of alleviating or reducing the effects of fatigue and weakness associated with cancer and cancer chemotherapy by administration of pharmaceutical compositions of the present invention.

Implanted catheters are locked with a solution comprising a lower alcohol, typically ethanol, propanol, or butanol, in a range from 1% to 99% by volume, and an additive in a range from 1% to 99% by volume, the additive comprising an anti-microbial, typically taurolidine or triclosan, or an anti-coagulant, typically riboflavin, sodium citrate, ethylene diamine tetraacetic acid, or citric acid. The use of an alcohol and additive solution can effectively reduce fouling of the catheter, particularly clotting and thrombus in intravascular catheters, as well as eradicate existing infections and / or reduce the risk of potential infections. Existing infections and / or potential infections can be further reduced by employing a catheter body which permits an anti-microbial solution to penetrate into the catheter body and preferably through the catheter into tissue surrounding the implanted catheter.

The present invention relates to a dietary supplement for the support of normal cerebrovascular tone. Extracts of the feverfew plant in combination with magnesium and riboflavin, either singly or in combination provide the major therapeutic enhancement in the reduction of migraine headaches and the associated symptoms.

The invention relates to a stable biochemical compound calibrator and a preparation method thereof and belongs to the technical field of biological in-vitro diagnosis. Through use of a compound protective substance composed of an excipient, an antioxidant, a surfactant, a stabilizer and a preservative, analytical components in the calibrator are stable in one month at the room temperature in dark,are stable in two years at 4 DEG C in dark and are stable in three years at -20 DEG C in dark. In preparation, a riboflavin photochemical method is used to inactivate various potential pathogenic microorganisms so that the calibrator has no potential pathogenic microorganism harmfulness. The stable biochemical compound calibrator contains more ingredients and greatly facilitates clinical use. Thepreparation method is traceable to international or domestic reference materials. The stable biochemical compound calibrator ensures clinical test result accuracy.

The invention relates to a feedstuff additive, in particular a feedstuff additive of rabbits; the additive is prepared scientifically and reasonably by combining with microelements, vitamins and amino acid, etc. which are needed in the processes of growing, reproducing, producing furs and producing meat, etc. for the rabbits; the microelements comprises copper, ferrum, zinc, manganese, cobalt, sulfur and halofuginone hydrobromide; the amino acid comprises methionine, lysine and arginine; the vitamins comprises A, E, D, K, B1, B12, riboflavin and biotin; kelp powder and iodine are added into the additive especially, thus solving the problems in the prior art that the additive of the rabbits has single formula and unreasonable proportion, which cause that the rabbits grow slowly with multiple diseases, and reproduction thereof occurs obstacles with low amount of fur and meat; the coccidiosis needs to be prevented, etc.; the invention provides a green feedstuff additive of the rabbits for rabbit-breeding families and large-scale rabbit-breeding warren for vast rural areas.

The invention discloses a method for treating riboflavin fermentation waste water, which comprises the following steps of: concentrating and drying the waste water after extracting riboflavin fermentation liquor by using a multi-effect evaporation system; drying concentrated dense size to obtain a large amount of solid protein feed; and putting condensed condensate water in a multi-effect evaporator into a biochemical system, and treating the condensate water by adopting an 'anaerobic upflow anaerobic sludge blanket (UASB), anaerobic / oxic (A / O) and contact oxidization decoloration' method. The method changes the conventional sewage treatment mode, effectively separates solid matters in riboflavin production waste water, reduces the sewage treatment difficulty, and solves sewage biochemical oxygen demand / chemical oxygen demand (B / C) values, thereby ensuring that the sewage can be biochemically degraded more easily and the waste water can be discharged up to standard. Simultaneously, the solid protein feed is recovered, and the up-to-standard waste water is recycled for blending, fermentation water replenishing, extracting working section and / or greening and the like, thereby saving water resources. The biogas generated by anaerobic treatment is collected for comprehensive utilization, thereby saving energy sources and realizing comprehensive utilization of resources.

The invention discloses a high-yield riboflavin Escherichia coli engineering strain, and a construction and fermentation method thereof. The construction method comprises the following steps: (1) obtaining a rib-int segment; (2) constructing a pLR 01 plasmid; (3) constructing an EC-rib 01 strain; and (4) constructing an EC-rib 02 strain, and knocking out the pfkA gene in the glycolytic pathway of the strain EC-rib 01 genome and the edd and eda genes in the ED pathway, thereby obtaining the strain EC-rib 02. The riboflavin yield of the high-yield riboflavin Escherichia coli engineering strain EC-rib 02 reaches 10.47 g / L or above, which is the maximum riboflavin yield for the existing Escherichia coli. The production cycle of the EC-rib 02 strain is 60-80 hours, which is shorter than the existing common riboflavin engineering strain production cycle, thereby saving the energy.

Trans-epithelial osmotic collyrium for the treatment of keratoconus by corneal cross-linking with UV rays, of the type consisting of a riboflavin-based solution and an absorption enhancement adjuvant consisting of benzalkonium chloryde (BAC). The solution has a hypoosmolarity degree ranging between −70% and −30% over an isotonic solution and contains a BAC concentration accordingly ranging between 0.005% and 0.035%.

The invention discloses a lactoflavin engineering bacterium and a constructing method thereof, and the preservation number of the lactoflavin engineering bacterium is CGMCC NO.16132. Through a geneticengineering means, a lactoflavin auxotroph strain is constructed, then a chromosome integrates and expresses a lactoflavin operon demodulated by bacillus amyloliquefaciens, and the lactoflavin production capacity of the strain is restored; then an expression vector overexpresses the lactoflavin operon demodulated by the bacillus subtilis, so that the lactoflavin production capacity of the straincan be improved; in addition, through point mutation of a ribC gene, the catabolism of lactoflavin is diminished; and finally, a recA gene which is responsible for homologous recombination and DNA repair on the chromosome is inserted for inactivation, and a lactoflavin production chassis cell is constructed. Based on the lactoflavin production chassis cell, through combination of the characteristic that the lactoflavin is a natural flroresence substance, and a strain high in lactoflavin yield is screened out through liquid drop micro-fluidic control. The invention also discloses a method for producing the lactoflavin through fermentation with the bacillus subtilis, and an application of the strain high in lactoflavin yield to lactoflavin production and an application of the strain high inlactoflavin yield to medicines, foods and feeds.

Biodegradable dextran based hydrogel suitable for biomedical application is produced by subjecting polysaccharide substituted with unsaturated moiety, e.g. dextran methacrylate, in aqueous medium to UV or visible light irradiation in the precense of riboflavin / L-arginine or riboflavin / chitosan to cause photocrosslinking of polysaccharide substituted with unsaturated moiety.

The invention relates to a production method capable of increasing yield of neomycin. According to the production method, one or several of additives which are lysine, ferrous lactate, tyrosine, methionine, hydrolysis casein, vitamin B12, fructose, glucosamine, folic acid, riboflavin and tryptophan are added to a conventional fermentation medium comprising peanut meal, corn starch, rice flour, yeast powder, glucose, peptone, ammonium sulfate, sodium chloride, light calcium carbonate and KH2PO4-containign streptomyces fradiae. The production method has the advantages that the yield of the neomycin is increased by above 5-20%, the cost is reduced, and the fermentation efficiency is high. The neomycin is used for treating human diseases, widely used in animal husbandries and used as antibiotics.

A pharmaceutical preparation comprising progestin, estrogen and a multivitamin agent. The progestin may be between 0 and 0.50 mg Desogesterel and the estrogen may be between 0 and 0.2 mg Ethinyl Estradiol. The multivitamin agent may be any combination of alpha carotene, beta carotene, biotin, bioflavonoid, calcium, chasteberry fruit, chromium, copper, coenzyme Q10, cryptoxanthin, dong quai root, folic acid, ginkgo biloba, garlic, grape seed extract, green tea extract, hesperedin, iodine, iron, lutein, malic acid, manganese, magnesium, milk thistle, molybdenum, niacin, panthothenic acid, potassium, pyridoxine HCL, quercetin, riboflavin, rutan, selenium, thiamine, vitamin A, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D, vitamin E, vitamin K, zinc, zoexanthin and any combination thereof.

The invention provides a natural pigment composition. The composition comprises an emulsifier, and two or more than two pigments selected from gardenia yellow pigment, curcumine, carthamin yellow, orange yellow pigment, xanthophylls, riboflavin, capsanthin, monascus red pigment, anattotree orange pigment and gardenia blue pigment. All the above natural pigments can be blended according to an arbitrary ratio to obtain the natural pigment composition with different color systems and different tones, such as yellow, orange, red, green and purple. The natural pigment composition contains the emulsifier, so the composition is uniform and stable. The combination of different natural pigments in the natural pigment composition makes up the defect of single pigments, for example, the light resistance of the natural yellow pigment composition obtained by combining riboflavin and curcumine with poor light resistance with stable gardenia blue pigment is better than that of riboflavin or curcumine, so the use amount is reduced, and the cost is reduced.

The invention discloses an industrial fermentation method of riboflavin, which comprises the following steps of: filling a seed culture medium into a 10m<3> seed tank, and performing oxic cultivation; and filling a fermentation medium into a 200m<3> fermentation tank for fermentation, mechanically stirring, adding sterilization starch glucose liquid, stopping fermenting till that the glucose is completely consumed or the dissolved oxygen is raised to be 60%, and extracting the riboflavin from the fermentation liquid. After the industrial fermentation method is used, the conventional riboflavin material-supplementing fermentation technology is changed, the low-concentration raw material cultivating bacteria is adopted, the material is quantificationally supplemented according to the changeof the dissolved oxygen of the fermentation solution, the excessive multiplication of the thallus can be effectively controlled, the deviation of the optimum control temperature caused by the excessive supersession can be avoided, the defects of the insufficient ventilation and oxygen supply can be remitted, and the thallus vigor can be kept, so that the indusial fermentation unit of the riboflavin is improved by more than 18g / L, and the yield is increased by 20% compared with the common fermentation.

The invention relates to a tumor tissue tumor infiltrating lymphocyte (TIL) cell preparation method and a dedicated culture medium. The method comprises the following steps of tumor surrounding tissue obtaining, cell digestion, cell primary culture, cell subculture and cell collection, wherein a primary culture medium is based on a RPMI 1640 culture medium and prepared from the following concentration ingredients of 10% volume of human-derived serum, 20 to 45ng / ml of basic fibroblast growth factor (bFGF), 1 to 5mg / ml of riboflavin, 70 to 90ng / ml of cortisol, 10 to 25mg / ml of sodium dihydrogen phosphate monohydrate, 47 to 62ng / ml of recombinant human leukaemia inhibitory factor (LIF) and 500 to 800U / ml of IL-2; a subculture medium is based on the RPMI 1640 culture medium and prepared from the following concentration ingredients of 10% volume of the human-derived serum, 20 to 40mmol / L of HEPES, 1000 to 2000U / ml of the IL-2, 0.03 to 0.07mmol / L of beta-mercaptoethanol and 5 to 15ng / ml of sodium phosphate. According to the preparation method, an existing culture medium is improved, different culture mediums are utilized to culture the TIL cells in pertinence, TIL cell expansion capacity is improved, meanwhile a culture period is reduced, a culture complexity degree is reduced, a use amount of the IL-2 is reduced, and toxic reaction is reduced.

The invention discloses a smart roe-fish ball product and a preparation method thereof, and belongs to the technical field of food production. The smart roe-fish ball product comprises the following raw materials: fish ball skin, fish ball stuffing, and brain-strengthening traditional Chinese medicine gelatin. The preparation method comprises the following steps: preparing smart roe-fish ball skin material and smart roe-fish ball stuffing in sequence, palletizing, cooking, shaping, cooling, speed freezing, packaging, and putting in storage, wherein during the process in preparing the fish ball stuffing, the brain-strengthening traditional Chinese medicine gelatin is added, and the brain-strengthening traditional Chinese medicine gelatin is prepared from dry walnut, dry fleece-flower root, dry fructus alpiniae oxyphyllae, dry fructus ligustri lucidi, dry boxthorn fruit, dry black mulberry seed, dry gingko leaf, fresh celery, and ice water. The smart roe-fish ball product richly contains nutrient substances including protein, calcium, phosphorus, iron, vitamin, riboflavin and cephalin, is good replenisher and growing agent for human brain and marrow, is very important for human, especially for growth and development of children, and has efficacies of marrow protection and brain strengthening.

In one embodiment, the present application discloses a cell culture medium for culturing cell lines suitable for producing a therapeutic protein, comprising an amino acid selected from a group consisting of L-arginine, L-asparagine, L-proline, L leucine and L hydroxyproline and a mixture thereof; a vitamin selected from a group consisting of ascorbic acid Mg2+ salt, biotin, pyridoxine HCL, folic acid, riboflavin and D-calcium pantothenate, and a mixture thereof; an element selected from a group consisting of ammonium meta vanadate, sodium meta vanadate, germanium dioxide, barium acetate, aluminum chloride, rubidium chloride, cadmium chloride, ammonium molybedate, stannous chloride, cobalt chloride, chromium sulfate, silver nitrate, sodium metasilicate, zinc sulfate, manganese sulfate H2O, manganous chloride, ferric nitrate 9H2O, ferrous sulfate 7H2O, ferric ammonium citrate, magnesium chloride anhydrous, and magnesium sulfate anhydrous, and a mixture thereof; a nucleoside selected from a group consisting of uridine and cystidine; a sugar selected from a group consisting of galactose, mannose and N-Acetyl-D-Mannosamine; and a triple buffering system comprising sodium carbonate, sodium bicarbonate and HEPES; wherein the cell culture medium is animal component-free, plant component-free, serum-free, growth factors-free, recombinant protein-free, lipid-free, steroid-free, and free of plant or animal hydrolysates and / or extracts.

The invention discloses a beautifying healthcare walnut cookie. The beautifying healthcare walnut cookie comprises, by weight, 50-60 parts of wheat flour, 40-50 parts of soybeans, 20-25 parts of walnuts, 2-3 parts of reed rhizome, 1-2 parts of cortex phellodendri, 1-3 parts of photinia serrulata leaves, 1-2 parts of caulis erycibes, 20-25 parts of lotus seed powder, 15-18 parts of yoghurt powder, 14-17 parts of bee pollen, 12-15 parts of brown sugar, 20-30 parts of pitaya, 1-2 parts of yeast, 1-2 parts of nutrition additives and an appropriate amount of water. The beautifying healthcare walnut cookie has the advantages that the wheat flour and the soybeans are used as main raw materials, and accordingly the beautifying healthcare walnut cookie is rich in nutrition elements such as carbonhydrate, protein and trace elements; the walnuts are added into the beautifying healthcare walnut cookie and contain rich protein, fat, carbonhydrate, various vitamin such as carotene and riboflavin and various trace elements and minerals which are necessary for human bodies and include calcium, phosphorus, iron and the like; effects of preventing hypertension and arteriosclerosis, caring brain and beneficiating intelligence further can be realized; comprehensive immune functions and the disease resistance of the human bodies can be improved owing to the bee pollen; effects of preventing senescence, beautifying, moisturizing and nourishing skin, promoting loss hair to gradually grow, keeping hair black and beautiful and the like can be realized.