235 results about "Neomycin" patented technology

The invention discloses a milk powder for calf, which comprises the following constituents (by weigh percent): whey powder 14-21%, whole milk powder 4-23.12%, puffed soybean 49-64%, concentrated soybean protein 1-3%, microelement premix 0.2-0.45%, vitamin premix 0.1-0.4%, calcium hydrogen phosphate 0.12-1.12%, table salt 0.03-0.06%, paunch accelerating agent 0.1-0.3%, intestinal tract protective agent 0.07-0.2%, neomycin 0.02-0.07%, terramicin 0.02-0.08%, anti-oxidizing agent 0.02-0.4%, lypressin 0.02-0.4%, hepionin 1-1.7%, tryptophan 0.5-0.75%, threonine 0.2-0.6%, stone powder 0.8-2% and fat 0.9-3%.

The invention discloses an up-conversion fluorescence immune chromatography test paper for the quantitative detection of neomycin and a preparation method thereof. The up-conversion fluorescence test paper comprises a support layer, an adsorption layer and a protection layer; the absorbing layer comprises an adsorption fiber layer, a fluorescent antibody fiber layer, a cellulose membrane layer and a water adsorption material layer at a handle hand; the cellulose membrane layer is provided with detection blotting printed by a carrier protein solution coupled with NEO and contrast blotting printed by a goat anti-mouse IgG; the fluorescent antibody adopts an NEO monoclonal antibody or polyclonal antibody marked by NaYF4:Yb:Er nanoparticles. Through the up-conversion fluorescence immune chromatography test paper for the quantitative detection of the neomycin, the application of the immune chromatography marked by up-conversion fluorescence nanometer materials in the quantitative detection of NEO residual is realized, so that the detection of the NEO residual is not subjected to background interference; the up-conversion fluorescence immune chromatography test paper for the quantitative detection of the neomycin is strong in specificity, high in sensitivity, simple, intuitional and accurate in detection, low in cost, wide in range of application and easy to popularize and apply.

The invention discloses a medical composite alginate dressing containing antibacterial drugs and a preparation method thereof. In parts by weight, the medical composite alginate dressing comprises the following components: 50-99.9 parts of alginate and 0.05-10 parts of antibacterial drugs, The antibacterial drug is selected from gentamicin or its salt, neomycin or its salt, amikacin or its salt, fusidic acid or its salt, kanamycin or its salt, polycresol , mupirocin, sulfamethonium or its salt and clindamycin or its salt at least one. The medical composite alginate dressing of the present invention has strong ability to absorb exudate, has good water vapor transmission rate, can be biodegraded and has good biocompatibility, does not adhere to the wound and can quickly form gel after absorbing wound exudate , has strong moisturizing properties, can effectively promote wound healing, shorten wound repair time, can effectively prevent or treat sensitive bacterial infections, and can be widely used in acute and chronic wounds with exudate.

The invention discloses a lentiviral vector for preparation of CAR-T (chimeric antigen receptor T cells), a construction method of the lentiviral vector and an application of the lentiviral vector inanti-tumor cell product preparation. Meanwhile, the invention further provides a method for detecting virus titer with fluorescence quantitative PCR (polymerase chain reaction), and detection standardis provided for clinical application. The lentiviral vector is short, can stably and efficiently express a CAR gene in T cells and contains no genes such as GFP, Neomycin and the like irrelevant to treatment, and a high-titer lentivirus can be formed through packaging by the lentiviral vector in viral packaging cells such as HEK293T. Experiments prove that the lentiviral vector is suitable for research and clinical application of CAR-T.

The invention discloses a measuring method for detecting residual quantities of streptomycin, dihydrostreptomycin, kanamycin, spectinomycin and neomycin in food simultaneously. The method comprises steps as follows: (1) extraction; (2) purification; (3) preparation of a matrix standard working solution; (4) measurement with LC-MS / MS (liquid chromatography-tandem mass spectrometry). The method has the advantages of simplicity in operation, accuracy, high sensitivity and good repeatability, can completely meet technical requirements for corresponding product security detection in China, the European Union, America and Japan and can provide powerful technical support for guaranteeing food security of people in China and sound development of export trades.

The invention relates to a production method capable of increasing yield of neomycin. According to the production method, one or several of additives which are lysine, ferrous lactate, tyrosine, methionine, hydrolysis casein, vitamin B12, fructose, glucosamine, folic acid, riboflavin and tryptophan are added to a conventional fermentation medium comprising peanut meal, corn starch, rice flour, yeast powder, glucose, peptone, ammonium sulfate, sodium chloride, light calcium carbonate and KH2PO4-containign streptomyces fradiae. The production method has the advantages that the yield of the neomycin is increased by above 5-20%, the cost is reduced, and the fermentation efficiency is high. The neomycin is used for treating human diseases, widely used in animal husbandries and used as antibiotics.

The invention discloses a recombinant plasmid and a construction method, the recombinant plasmid contains an antioxidation reaction element, luciferase gene and neomycin resistant gene. The invention also discloses a cell line screened by an anti-oxidant or a chemical preventive agent, a construction and an application, and the cell line is the cell integrated with the above recombinant plasmid. The provided cell line integrates the ARE gene in a recombinant plasmid carrier pARE-Luc-Neo, luciferase gene and neomycin resistant gene to a genome of a host cell Hek293, by following with subcultring of cell (more than 10 generation), the detection of a luciferase expression is stable, compared with a transient transfection method, and the construction provided by the invention is more convenient, sensitive and reliable.

The invention provides an enzyme linked immune regent box to detect neomycin medicine. It includes: the enzyme labeled board which encrusts the encrusting substance, the enzyme marker, the special antibody for the neomycin, the neomycin standard solution, the substrate color solution, the stop solution, the condense washing solution, the condense recovery solution. The invention also discloses the method which includes the process: first to pre-treat the sample, then to detect by the regent box and last to analyze the detecting result. The invention is to detect the neomycin residue weight in the animal food such as the chicken, chicken liver, pork, pork liver, milk powder, egg and feedstuff. The method is simple, low cost and has high sensitivity, also it is proper for detect the mass sample.

The invention relates to a one-step ELISA (Enzyme Linked Immunosorbent Assay) method for neomycin (NEO) residues in milk, which belongs to the technical field of immunoassay chemicals. The method comprises the following steps of: coupling NEO with bovine serum albumin (BSA) or ovalbumin (OVA) to synthetize a NEO antigen (immunogen NEO-BSA or coating antigen NEO-OVA) by a glutaraldehyde method; authenticating the NEO-BSA by utilizing an infrared spectroscopy and an SDS-PAGE (Sodium Dodecyl Sulfate-PolyAcrylamide Gel Electrophoresis) method; obtaining NEO antiserum by immunization and detectingthe valence of the NEO antiserum by an indirect ELISA method; and detecting the specificity of the NEO antiserum by the one-step ELISA method. Compared with the indirect competition ELISA method, theinvention greatly shortens the detecting time of the specificity of the NEO antibody, has higher sensitivity, achieves the aim of simply, rapidly and sensitively detecting NEO residues in milk and can be widely used for rapid field detection.

The invention relates to a sterile cultivation method for algae, in particular to a sterile cultivation method for enteromorpha. Firstly, enteromorpha seedlings are washed 2-3 times, and the surfaces of the enteromorpha seedlings are absorbed dry; secondly, three antibiotics including the penicillin G, the neomycin and the polymyxin B are added into a VSE nutrient solution to be prepared into an antibiotic nutrition solution; thirdly, the enteromorpha seedlings are placed in the antibiotic nutrition solution, the cultivation temperature ranges from 18 DEG C to 25 DEG C, the illumination intensity ranges from 130mumol / m<2>*s, the photoperiod is 12L:12D, the enteromorpha seedlings are cultivated in an inflation mode, and the antibiotic nutrition solution is replaced every 3-6 days until the enteromorpha seedlings grow into mature algae. The sterile cultivation is carried out on the enteromorpha collected in the field in a laboratory, the enteromorpha is tested and has no infectious microbe growth, and accordingly the accuracy of the later experiment results of the enteromorpha is greatly improved. The sterile cultivation method is easy to operate, the enteromorpha cultivation system does not need to be changed, raw materials are easy to obtain, price is low, and cost is low.

The invention discloses odontobutis obscura parent fish feed. The odontobutis obscura parent fish feed is composed of the following components in parts by weight: 40-60 parts of eel grass, 40-60 parts of duckweed, 30-50 parts of corn meal, 10-20 parts of globe artichoke, 5-10 parts of hizikia fusiformis, 5-15 parts of potamogeton malaianus, 1-5 parts of pomegranate peel extract, 1-5 parts of persimmon leaf extract, 5-10 parts of crabshell powder, 5-10 parts of earthworm powder, 5-10 parts of pumpkin powder and 1-2 parts of sulphur neomycin. The odontobutis obscura parent fish feed has the advantages that the formula of raw materials is reasonable, a preparation technology is simple and feasible, the content of total protein in feed is high, energy required by growth of a parent fish can be provided, and egg laying amount is high; globe artichoke, potamogeton malaianus, hizikia fusiformis, pomegranate peel extract, persimmon leaf extract and sulphur neomycin are compounded, antibacterial and antiviral capabilities of the parent fish are improved, growth and development of the parent fish are promoted, diseases are fundamentally prevented and controlled, and the survival rate of fish fries is increased; palatability can be enhanced by virtue of the crabshell powder, the earthworm powder and the pumpkin powder, and the grazing rate of the parent fish is increased.

The invention discloses a neomycin immuno-colloidal gold detection card and a preparation method thereof, and relates to the technical field of beta-adrenergic receptor agonist detection. The test strip on the shell of the detection card is composed of a PVC rubber sheet, a sample pad, a colloidal gold combined pad, a coating membrane, and a water absorbing pad; the colloidal gold membrane is a glass cellulose membrane containing a neomycin monoclonal antibody; the coating membrane is a nitrocellulose membrane and is provided with a T line and C line, neomycin protein conjugate is coated in the T line, and goat anti mouse IgG antibody is coated in the C line. The provided detection card is used to detect neomycin in a short time, and has the advantages of convenience, quickness, and precise results.

The invention discloses a method for simultaneously analyzing various aminoglycoside compound residues in agricultural and animal products. The method comprises the following steps: weighing a sample;adding an ammonium acetate buffering solution; after carrying out oscillation and extraction, carrying out centrifuging; separating an extracting solution obtained by centrifuging and regulating to asuitable range by utilizing a PH (Potential of Hydrogen) meter; purifying by adopting dispersed solid-phase extraction and a solid-phase extraction small column. Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS), electrospray ionization (ESI) and multi-reaction monitoring (MRM) scanning modes are adopted. Compared with other similar methods, the method disclosed by the invention has the advantages of simplicity in operation and high sample flux; eight types of aminoglycoside compounds including streptomycin, dihydrostreptomycin, spectinomycin, gentamicin, apramycin, kanamycin, amikacin and neomycin are detected at the same time; the detection efficiency is greatly improved, and rapid qualitative and quantitative detection can be rapidly carried out; the detection limit can completely meet domestic and overseas laws and regulations and limitation requirements, and powerful support is provided for food safety guarantees and import and export of the agricultural and animal products of China.

This invention is for an improved process to co-encapsulate hydrophobic drugs and hydrophilic drugs in phospholipid liposomes. Non-toxic supercritical or near-critical fluids with / without polar cosolvents are utilized to solubilize phospholipid materials and hydrophobic drugs, and form uniform liposomes to encapsulate hydrophobic drugs and hydrophilic drugs.DNA topoisomerase I (Top1) is the target of camptothecin, and novel Top1 inhibitors are in development as anticancer agents. Top1 inhibitors damage DNA by trapping covalent complexes between the Top1 catalytic tyrosine and the 3′-end of the broken DNA. Tyrosyl-DNA phosphodiesterase (Tdp1) can repair Top1-DNA covalent complexes by hydrolyzing the tyrosyl-DNA bond. Inhibiting Tdp1 has the potential to enhance the anticancer activity of Top1 inhibitors and to act as antiproliferative agents. It has been recently reported that neomycin inhibits Tdp1 more effectively than the related aminoglycosides paromomycin and lividomycin A. Inhibition of Tdp1 by neomycin is observed both with single- and double-stranded substrates but is slightly stronger with duplex DNA, which is different from aclarubicin, which only inhibits Tdp1 with the double-stranded substrate. Inhibition by neomycin can be overcome with excess Tdp1 and is greatest at low pH. Aminoglycoside antibiotics and the ribosome inhibitors thiostrepton, clindamycin-2-phosphate, and puromycin are the first reported pharmacological Tdp1 inhibitors. The development of Tdp1 inhibitors as anticancer agents can be envisioned as combinations of Tdp1 and Top1 inhibitors. Moreover, Tdp1 inhibitors might also be effective by themselves as anticancer agents. In addition, Tdp1 inhibitors might be valuable as anti-infectious agents.This invention can produce a co-encapsulated combination drug product consisting of a topoisomerase 1 inhibitor such as camptothecins including neat camptothecin and its derivatives irinotecan, topotecan and other derivatives, and a tyrosyl-DNA phosphodiesterase (Tdp1) such as aminoglycoside antibiotics including neomycin and tetracycline, and the ribosome inhibitors thiostrepton, clindamycin-2-phosphate, and puromycin.

The invention provides a method for simultaneous determination of multiple nutrient compositions and antibiotic titer changes by using near infrared spectroscopy during a production process of antibiotics from microbial fermentation. The method comprises the following steps: spectrums of antibiotic (including aureomycin, terramycin, neomycin and other fermentation type antibiotics) fermentation liquor in different fermentation periods are acquired by using the near infrared spectroscopy technology; each acquired sample is detected by a chemical method, and physical and chemical data of multiple nutrient composition contents and antibiotic titer changes are obtained; the acquired near infrared spectrums as well as the physical and chemical data are fitted for establishing a model. After verification, the model is suitable for detecting nutrient composition contents and antibiotic titer changes in antibiotic fermentation liquor in different periods. The method is used for rapidly feeding back and understanding fermentation cases, timely carrying out nutrition supplement and process control, and monitoring antibiotic titer changes; the whole detection process is highly efficient and saving without pollution.

The invention discloses a specific monoclonal antibody capable of identifying neomycin, amikacin and paromomycin and an enzyme-linked immunosorbent assay (ELISA) method and kit for detecting neomycin, amikacin and paromomycin. The monoclonal antibody is secreted by a hybridoma cell EDC / 5G04 and the hybridoma cell is collected in the China Center for Type Culture Collection, with collection number being CCTCC NO:C201144. The ELISA method comprises the steps of preparation of immunogen, coating antigen and the antibody, treatment and detection of samples and the like. Compared with the prior art, the monoclonal antibody, the ELISA method and the kit have the following main advantages that the prepared monoclonal antibody can simultaneously identify neomycin, amikacin and paromomycin, thus improving the detection efficiency of the prior art; the animal tissue sample treatment method is simple and short in time; and the ELISA method and the kit also have the characteristics of high detection sensitivity, good precision and good accuracy.

The invention discloses a molecular engram integral cylinder and its process method for detecting gentamicin, streptomycin and neomycin residue in animal food.The method adopts gentamicin, streptomycin and neomycin as molecular modular and combining in original position to process gentamicin, streptomycin and neomycin molecular engram integral cylinder. The stuff in cylinder is a crosslinked polymers of determined range functional group with fixed hole size and shape, which has memory function for modular molecular spatial structure of gentamicin, streptomycin and neomycin.

An ointment composition for treating decubitus ulcers and methods for its making and its use. The composition includes a skin protestant ointment, a rash cream, an antibiotic ointment, virgin olive oil, and boric acid powder. The skin protestant ointment includes active ingredients petroleum 53.4%, lanolin 15.5%, and inactive ingredients cod liver oil containing vitamin A & vitamin D, a fragrance, light mineral oil, microcrystalline wax, and paraffin. The rash cream includes active ingredients dimethicone 1% and zinc oxide 10%, and inactive ingredients aloe barbadensis extract, benzyl alcohol, coconut oil, cod liver oil containing vitamin A & vitamin D, a fragrance, glycerol oleate, light mineral oil, ozokerite, paraffin, propylene glycol, sorbitol, synthetic beeswax, and water. The antibiotic ointment includes active ingredients polymyxin B sulfate 5,000 units, bacitracin zinc 400 units, and neomycin base (as sulfate) 3.5 mg., and an inactive ingredient white petroleum.

The invention relates to the microbial field, and discloses corynebacterium glutamicum strains and application thereof. A corynebacterium glutamicum MHZ-0510 strain has a preservation number CGMCC No.7939; a corynebacterium glutamicum MHZ-0511 strain has a preservation number CGMCC No.7940; the two strains have significantly improved ability of fermentation for generating L-glutamine, compared with that of the original strain of wild type of corynebacterium glutamicum B498, and can be widely applied to fermentation production of L-glutamine. The corynebacterium glutamicum strains involved in the invention are obtained by screening aminoglycoside antibiotics, such as streptomycin and neomycin, have resistance to the aminoglycoside antibiotics including streptomycin and neomycin, and have improved ability to synthesis of L-glutamine, thus increasing the yield of L-glutamine. Therefore, aminoglycoside antibiotics can be widely applied to screen of L-glutamine.

The invention relates to an enzyme-linked immunodetection method of aminoglycoside antibiotics in animal-derived foods, belonging to the immnunoassay field. The invention utilizes a carbodiimide method to synthesize immunogen (NM)L-(KM)m-(GM)n-(SM)k-BSA with multiple antigen determinants, such as aminoglycoside antibiotics, by four steps, utilizes a glutaric dialdehyde method to synthesize the envelope antigen of the aminoglycoside antibiotics and establish the enzyme-linked immunodetection method of the aminoglycoside antibiotics in the animal-derived foods; a polyclonal antibody with the specificity of aminoglycoside cluster is adopted as a detection reagent; gentamicin, neomycin, kanamycin or streptomycin is adopted as a standard substance, the four antibiotics and OVA conjugate are adopted as the envelope antigens, and the establishment of an ELISA method for detecting multi-residue of the aminoglycoside antibiotics provides a detecting means with high speed and high efficiency for the multi-residue detection of the aminoglycoside antibiotics in the animal-derived foods. The invention has the advantages of simple pre-processing, high sensitivity, high precision and the like.

The invention discloses a spectinomycin, streptomycin, gentamycin and neomycin detection test strip and a method thereof. The test strip comprises micro-porous strips, micro-porous reagents, a test paper and micro-porous plugs, the micro-porous reagents are lyophilized colloidal gold labeled spectinomycin, streptomycin, gentamycin and neomycin specific antibodies respectively, the test paper is formed by sequentially connecting a sample absorption pad, a reaction film, a water absorption pad, a protection film and a substrate, the reaction film comprises four detection lines and a quality control line, the four detection lines are coated with a spectinomycin-carrier protein conjugate, a streptomycin-carrier protein conjugate, a gentamycin-carrier protein conjugate and a neomycin-carrier protein conjugate respectively, and the quality control line is coated with an antiantibody. The method for detecting the spectinomycin, streptomycin, gentamycin and neomycin by using the test strip has the advantages of simplicity, rapidness, perceptual intuition, accuracy, portability, wide application range, low cost, and easy popularized use.

The invention discloses a fermentation medium used in the neomycin production adopting a fermentation method. The fermentation medium comprises an organic carbon source, an organic nitrogen source, inorganic salt and amylase, wherein the concentration of the organic carbon source is 60-110 g / L. After neomycin is produced by using the fermentation medium, the fermentation unit of the obtained neomycin can reach more than 33,310 U / mL, so that the profits of neomycin production enterprises can be greatly increased and the fermentation medium has a broad application prospect.

The invention discloses a neomycin semi-quantitative gold-labeled rapid detection kit, and a detection method and an application thereof, and belongs to the field of immunology. The kit includes a sample diluent, an enzyme label plate and neomycin gold-labeled rapid detection strips; each detection strip is composed of a sample pad, a gold-labeled antibody combination pad, a nitrocellulose film and a water absorption pad; the gold-labeled antibody combination pad is a neomycin monoclonal antibody labeled by colloidal gold; two detection lines and a quality-control line are coated at the middle part of the nitrocellulose film; a reaction of the neomycin antibody and an neomycin antigen is showed in a colored reaction mode through a colloidal gold immunochromatography technology, and during the detection process, whether neomycin residues exist and a residual amount range in dairy products are determined through changes of colors of the two detection lines. The detection kit can achieve semi-quantitative detection of the residues, has the advantages of simple detection method, short detection time, low detection cost, wide detection range, accurate and reliable detection results, and can be widely applied to neomycin semi-quantitative detection in food.

The invention aims to provide an animal semen preservative with biological activity and a preparation method of the animal semen preservative. The animal semen preservative with the biological activity contains oligoprocyanidine so that the animal semen preservative has an anti-oxidation effect, can prolong the preservation time of semen and keeps the activity of animal sperms. The animal semen preservative is prepared by mixing 1000mL of a common medium and 16mg of oligoprocyanidine; the common medium is prepared by mixing 6 parts of glucose, 0.37 part of EDTA (Ethylene Diamine Tetraacetic Acid), 0.375 part of sodium citrate dihydrate, 0-0.12 part of sodium bicarbonate, 0.1 part of neomycin and 100 parts of distilled water; the neomycin is independently added before the semen is diluted. The formula contains the oligoprocyanidine so that the animal semen preservative has the anti-oxidation effect and can prolong the preservation time of the semen; the degradation of a semen sample can be relieved by the oligoprocyanidine and the oligoprocyanidine has no negative effects on the activity of the sperms, so that the activity of the animal sperms is kept; particularly, the survival rate of the sperms preserved at a low temperature is obviously improved.

The invention discloses a time resloved fluoroimmunoassay (TRF) kit for detecting Galectin-3.The kit comprises: a coating buffer, a blocking solution, a cleaning solution, a loading buffer, a enhancement solution, a assay buffer, coated antibody (monoclonal anti-human Galectin-3 antibody), detection antibody (biotinylated anti-human Galectin-3 antibody), Eu<3+> labeled streptavidin and neomycin, a standard substance (recombinant human Galectin-3). The kit is characterized in that: the kit adopts a double antibody sandwich method and TRF principle for detecting a concentration of the Galectin-3 in a sample. The method is characterized by: coating an ELISA plate through the coated antibody; adding the sample to be detected to the ELISA plate to carry out a incubation after blocking; washing the plate, then adding the detection antibody to the plate to carry out the incubation; washing the plate again, followed by adding the Eu<3+> labeled streptavidin to carry out the incubation; washing the plate, followed by adding the enhancement solution; oscillating for 5 minutes, followed by exciting through light having a excitation wavelength of 337nm; detecting a fluorescence value of the sample at a emission wavelength of 615nm after delaying 200 microseconds; drawing a standard curve through the concentration of the standard substance and the corresponding fluorescence value, such that the concentration of the Galectin-3 in the sample to be detected can be calculated through the fluorescence value and the standard curve.

The invention provides a fermentation process of neomycin. Cant spore suspension is put in a seed tank with pre-accommodated with a seed culture medium for cultivating 36-48 hours to obtain a culture solution; the seed culture medium in the seed tank contains soybean meal; the obtained seed culture solution is fed in a fermentation tank pre-accommodated with a fermentation culture medium for inoculating and fermenting 110-130 hours; a sugar material is supplemented in the fermentation process; the fermentation of the seed culture solution is realized through the phase control of air dosage and the volume control of fermentation solution; the cultivation of the fermentation solution is terminated; the fermentation solution is put in an absorption tank; 732 resins are added in the fermentation solution; the concentration of the fermentation solution is diluted below 13,000 u / ml; the pH value is neutralized within 6.4-7.0; then dilute fermentation solution is prepared; the fermentation steps are finished. The process improves the fermentation level above 15%, prominently creates the economic benefit, reduces the fermentation cost, and can be applied to the industrial production in large quantities.

The invention discloses a method for producing neomycin by replacing part of fermenting raw materials with soybean meal. The method consists of the following steps: (1) culturing bevel spores; (2) culturing a bevel spore suspension; (3) performing seeding tank culturing: putting the bevel spore suspension into a seed culture tank for culturing; (4) performing fermentation tank culturing: feeding a culture solution subjected to the seed tank culturing into a fermentation culture tank for culturing, wherein the culture solutions in the seed tank and the fermentation tank include soybean meal of which the mass content is 2.0-5.0 percent the mass of the culture solution in the fermentation tank, and the culture solution in the fermentation tank does not contain peptone, yeast powder and peanut powder; (5) terminating fermentation, diluting a fermentation solution, neutralizing till the pH value becomes 6.4-7.0 to obtain a diluted fermentation solution, thereby finishing the fermentation step. According to the method for producing the neomycin by replacing part of the fermenting raw materials with the soybean meal, the problems of complex raw material formula and high cost of the culture solution are solved, the formula is simplified effectively, the cost is reduced, and the production of the neomycin is guaranteed.

The invention relates to a culture medium for producing neomycin by utilizing fermentation of streptomyces fradiae. The culture medium comprises a seed culture medium and a fermentation culture medium, wherein soy peptone, biological fish meal, millet meal, zinc lactate, ammonium molybdate and other substances are added into the culture medium, so that metabolism of strains can be benefited, fermentation starting titer can be improved, the aging speed of hyphae can be delayed, the neomycin production capability can be improved, the fermentation cycle can be shortened, the fermentation cost can be reduced, and high-efficiency production of neomycin can be realized.

An expression carrier for effectively screening target protein is prepared through introducing an internal ribosome to the eukaryotic expression carrier containing CMV promoter and dhfr amplification system, linking the fusion gene of anti-erbB2-seFv-Fc gene and IL-2 to the neomycin-resistant gene, site mutation to Neo gene by PCR, and configuring the expression carrier pCMV-HFI-mNeo.