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Neomycin semi-quantitative gold-labeled rapid detection kit, and detection method and application thereof

A detection kit and neomycin technology, applied in the field of immunology, can solve the problems of inability to realize semi-quantitative detection of residues, large application limitations, cumbersome detection methods, etc., and achieve simple pretreatment methods, low detection costs, and high detection efficiency. short time effect

Inactive Publication Date: 2014-04-23
北京陆桥技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] To sum up, the application of existing immunochromatography technology still has defects such as the inability to realize the semi-quantitative detection of residues, the detection method is cumbersome, the detection time is long, and the application limitations in actual work are large.

Method used

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  • Neomycin semi-quantitative gold-labeled rapid detection kit, and detection method and application thereof
  • Neomycin semi-quantitative gold-labeled rapid detection kit, and detection method and application thereof
  • Neomycin semi-quantitative gold-labeled rapid detection kit, and detection method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] The sensitivity experiment of embodiment 1 kit

[0074] For neomycin residue limit in milk stipulated in 235 Announcement of the Ministry of Agriculture is 400ng / kg, currently there is no national standard to refer to, only in GB / T21323-2007 (Determination of aminoglycoside drug residues in animal tissues with high-efficiency liquid phase chromatography-mass spectrometry), but this method is only applicable to the detection of neomycin residues in animal tissues. The Dutch EURO-PROXIMA Neomycin Detection Kit can be used to detect the drug residues in milk samples, with a detection sensitivity of 18ng / ml, which can meet the screening requirements for negative samples.

[0075] Take 5 milk samples that are free of aminoglycoside antibiotics tested by the Netherlands EURO-PROXIMA neomycin detection kit and the GB / T22969-2008 national standard method, and add different concentrations of standard substances (Table 1). Each sample was repeatedly measured 5 times, and the low...

Embodiment 2

[0080] Embodiment 2 detection strip specificity determination

[0081] The so-called specificity of the test strip refers to whether the test strip has cross-reaction with other drugs. In this experiment, several common veterinary drug standard products were selected for the addition test (see Table 2). The final concentrations of all drugs were 0.5 and 1ug. / ml. The test results show that there is no cross-reaction between the neomycin gold-labeled rapid test strip and other drugs, and it has a high degree of specificity. .

[0082] Table 2 The detection results of neomycin gold-labeled rapid test strips

[0083]

[0084]

[0085] Note: "-" indicates that the test result is negative, and "+" indicates that the test result is positive.

Embodiment 3

[0086] The preparation of embodiment 3 rapid detection test strips

[0087] 1. Preparation of gold-labeled antibody binding pad

[0088] 1. Preparation of blank colloidal gold:

[0089] The colloidal gold particle is the 25nm colloidal gold solution that adopts trisodium citrate reduction method to prepare, and specific method is: add 97.5ml double-distilled water and 1ml1% chloroauric acid solution in the Erlenmeyer flask with magnetic stirring bar and heat to boiling and continue After boiling for 4 minutes, add 1.5mL of 1% trisodium citrate solution, continue to boil until the liquid turns orange, keep boiling for 10 minutes, then stop heating, take out the stirring bar in the flask, fully cool the solution at room temperature, and refrigerate for later use;

[0090] 2. Preparation of colloidal gold-labeled antibody:

[0091] Take 100ml of blank colloidal gold solution, add 425μL of potassium carbonate solution with a concentration of 0.1g / L, and stir slowly at a constant...

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Abstract

The invention discloses a neomycin semi-quantitative gold-labeled rapid detection kit, and a detection method and an application thereof, and belongs to the field of immunology. The kit includes a sample diluent, an enzyme label plate and neomycin gold-labeled rapid detection strips; each detection strip is composed of a sample pad, a gold-labeled antibody combination pad, a nitrocellulose film and a water absorption pad; the gold-labeled antibody combination pad is a neomycin monoclonal antibody labeled by colloidal gold; two detection lines and a quality-control line are coated at the middle part of the nitrocellulose film; a reaction of the neomycin antibody and an neomycin antigen is showed in a colored reaction mode through a colloidal gold immunochromatography technology, and during the detection process, whether neomycin residues exist and a residual amount range in dairy products are determined through changes of colors of the two detection lines. The detection kit can achieve semi-quantitative detection of the residues, has the advantages of simple detection method, short detection time, low detection cost, wide detection range, accurate and reliable detection results, and can be widely applied to neomycin semi-quantitative detection in food.

Description

technical field [0001] The invention belongs to the field of immunology, and relates to a kit and a method for rapidly detecting residues of aminoglycoside antibiotic neomycin in food. Background technique [0002] With the development of the economy and the improvement of people's living standards, people's requirements for food are not simply to fill their stomachs, but to pay more attention to the nutrition and safety of food. Because dairy products contain a lot of vitamins and minerals in addition to high-quality protein, fat, and carbohydrates, they are recognized as an ideal food so far, so milk and dairy products have become people's favorite daily food. one. [0003] From the perspective of the general environment, it is expected that in the next three years, China's urbanization rate will be further strengthened. By 2013, China's urban population may exceed the rural population, and China will become a country where urban consumption drives national consumption. ...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/577G01N33/531G01N33/558
Inventor 王丽哲唐慧林何艳玲赵瑜王丽丽于重楠刘敏李瑾
Owner 北京陆桥技术股份有限公司
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