52results about How to "Achieve semi-quantitative detection" patented technology

The present invention relates to a colloidal gold semiquantitative immunologic diagnosis test paper preparing and assembly method. Said test paper utilizes immunity chromatograph principle capable of rapid visual inspection nano colloidal gold marker and sample combined colour, judging detective semiquantitative result through test zone with parallel reference zone colour contrast.

The invention discloses a Sudan I immune chromatography test paper detection method. On the back liner of the test paper, a sample liquor absorption part, a colloidal gold marking part, a detecting reaction part and a water absorption part are sequentially pasted, wherein, the detecting reaction part is covered with one or two strips of Sudan I polyclonal antibodies or one or two strips of antigens used for detecting, which are used as detecting lines, meanwhile, one strip of IgG which resists the second species animal protein also covers the detecting reaction part and is used as a reference line. The combination mode has two ways, that is, one detecting line plus one reference line or two detecting lines plus one reference line. The fast detection test paper can be used when the temperature ranges from 4 DEG C to 40 DEG C and is suitable for fast detecting foods such as meat, etc. which may be polluted by Sudan I by departments such as sanitary, quality supervision, customs and supermarkets, etc. The Sudan I immune chromatography test paper detection method has the advantages of strong specificity, high sensitivity, capability for realizing semi-quantitative detection, simple and convenient operation, etc.

The invention discloses a kit for detecting protein glycosylation subtype and a detection method thereof. The detection kit comprises agglutinin, confining liquid, a biotin labeled antibody, horse radish peroxidase labeled streptavidin, a 3',3',5',5'-tetramethyl benzidine substrate developing solution, a stop solution and a cleaning buffer solution. The detection method comprises the following steps: fixing the agglutinin onto a polystyrene solid phase carrier to be used for recognizing and binding glycoprotein in the sample based on specific recognition of agglutinin and an oligosaccharide chain, recognizing the glycoprotein needing to be detected by virtue of specific binding of the protein and the antibody thereof, and realizing semi-quantitative analysis of the glycoprotein. According to the detection kit and the detection method disclosed by the invention, semi-quantitative detection of single protein glycosylation subtype can be realized, and the kit is easy and convenient to operate and low in cost.

The invention discloses an NTx (Naltrexone) detection test strip. The NTx detection test strip comprises a chromatography membrane and a conjugate pad; an immunolabelled NTx antibody and an immunolabelled first antibody are loaded on the conjugate pad; the chromatography membrane is provided with an NTx protein covered detection line and a second antibody covered colorimetric line along a liquid chromatography direction. When a sample is detected, the colorimetric line correspondingly displays the chroma of specific protein concentration after the second antibody is specifically combined withthe first antibody; NTx protein on the detection line and the NTx protein in the sample are competitively combined with the immunolabelled NTx antibody and the color developing depth of the detectionline and the colorimetric line is directly compared so that semi-quantitative detection of the NTx protein is realized. The invention discloses a preparation method of the NTx detection test strip; the preparation method is used for preparing an NTx protein semi-quantitative detection test strip which is rapid and sensitive in detection and does not depend on an instrument. The invention disclosesan NTx detection kit; the NTx detection kit comprises the NTx detection test strip and can provide effective information for early diagnosis and treatment of osteoporosis.

The invention discloses a visual rapid combined measuring method of plant pathogen and an antibody chip. The method provided by the invention comprises the following steps of: distributing plant pathogen grabbing antibody points on basal lamina to obtain an antibody chip, hybridizing a detected sample and the antibody chip for incubation,washing, adding a corresponding plant pathogen detection antibody, continuously reacting, washing and developing. The method provided by the invention has the same sensitivity, specificity and reproducibility with methods such as ELISA and the like, and also has the capability of detecting a plurality of pathogens in one experiment, which is what traditional methods don't have.

The invention discloses a semi-quantitative detecting paper of Lac-dopamine effluent immune chromatography, which comprises the following parts on the test paper back: sample liquid absorption part, colloidal gold mark part, detecting reaction part and water-absorbing part, wherein the detecting reaction part contains 1-3 Lac-dopamine antibody as detecting line, which contains 1-3 anti-second animal protein lgG as reference line simultaneously; the amount of combined detecting line and reference line isn't more than one simultaneously; the amount of combined line is 2-4. The invention displays strong specificity to accomplish semi-quantitative detection at 4-40 deg.c temperature for 3 min, which is fit for unit or personnel to detect the Lac-dopamine medicine in the animal source food sample rapidly.

The invention discloses a semiquantitative immunodiagnosis NGF colloidal gold test paper and a preparation method thereof. The semiquantitative immunodiagnosis NGF colloidal gold test paper is separated into a first sample absorption area, a second immobilization colloidal gold mark NGF polyclonal antibody area, an third immobilization another NGF polyclonal antibody area, a fourth immobilization NGF area and a fifth water absorption area from bottom to top, wherein spaced narrow slits are symmetrically distributed on both sides of the third immobilization another NGF polyclonal antibody area and the fourth immobilization NGF area. The semiquantitative immunodiagnosis NGF colloidal gold test paper is characterized in that the fourth immobilization NGF area comprises a clutter signal removing stripe and 2 to 7 NGF stripes with degressive concentration. Through the semiquantitative immunodiagnosis NGF colloidal gold test paper, the color of nanometer colloidal gold markers can be rapidly visually inspected after the nanometer colloidal gold markers are combined with samples and a detected semiquantitative result can be judged according to the comparison of color at the left sides and the right sides of testing areas and the number of the strips of a right side area by adopting an immunochromatographic principle. The semiquantitative immunodiagnosis NGF colloidal gold test paper has the characteristics of reacting rapidly and rapidly judging if the samples have objects to be tested and the amount of the content of the objects to be tested.

The invention discloses a colloidal gold immunochromatography test strip used for detecting human NGAL, a kit, and a preparation method of the colloidal gold immunochromatography test strip and the kit. The colloidal gold immunochromatography test strip comprises a substrate, and a sample pad, a colloidal gold marked pad, a coating membrane, and water absorption paper which are arranged on the substrate successively; the colloidal gold marked pad is coated with colloidal gold marked anti-human NGAL detection antibody and colloidal gold marked chicken IgY antibody; the coating membrane comprises a detection zone and a reference zone which are arranged in parallel and are separated from each other; the detection zone is coated with anti-human NGAL coating antibody; the reference zone is coated with goat-anti-chicken IgY antibody; the anti-human NGAL detection antibody possesses epitopes corresponding to an amino acid sequence represented by SEQ ID No:5. The colloidal gold immunochromatography test strip is simple, rapid, practical, and sensitive, is high in efficiency, is suitable for clinical rapid detection and on-site rapid detection, is convenient to store, and is suitable to be popularized and applied in primary hospitals, and batch production can be realized.

The invention relates to a melamine immunity-chromatography test strip test method, comprising: the liner of the test strip is attached with a sample solution absorption part, an adhesive gold mark part, a test reaction part and a water adsorption part in turn, wherein the test reaction part is enveloped by one melamine multi-clone antibody or one detection antigen as the detection line and is enveloped by one IgG resisting the second animal protein as the reference line. The fast detection test strop has strong specificity, can realize half-quantitative detection, can be used in the environment temperature of 4 to 40 DEG C, is suitable for the fast test on the food and feeds of melamine pollution for milk product enterprises, individual cultivators, food health quality monitor departments, customs and supermarkets and the like. The melamine immunity-chromatography test strip test method has the advantages of strong specificity, high sensitivity, half-quantitative test and simple operation and the like.

The invention provides gold labeled immunochromatographic strip assay semiquantitative detection test paper for detecting dienestrol. The test paper is characterized in that a sample absorption pad, a colloid gold label pad, a chromatography pad and a water absorption pad are pasted on the back lining of the test paper; and a detection reaction pad is covered by dienestrol antigen serving as a detection line and second antibody serving as a quality control line at the same time. The semiquantitative detection test paper has the advantages that the test paper has strong specificity and can realize semiquantitative detection; the test paper can be used at a temperature of between 4 and 40 DEG C and can obtain a result after 5 minutes; and the test paper is suitable for quickly detecting dienestrol drugs in animal-derived foodstuff samples by sanitation departments, quality inspection departments, customs and livestock farms and foodstuff enterprises.

The invention relates to a culture-independent semiquantitative detection kit of tilapia streptococcus agalactiae, which consists of a reagent A, a reagent B, a reagent C, a reagent D, a reagent E, a product specification and a test result record card. The kit can be used for directly detecting whether blood of tilapia carries the streptococcus agalactiae without pure culture by a method provided by the invention, and semiquantitatively detect the quantity of the streptococcus agalactiae in the blood.

The invention discloses a detection method of the content of lead chrome green in tea leaves. The method comprises the steps: taking tea juices obtained by soaking tea leaves with different lead chrome green contents as test samples, and acquiring Raman spectra of all the test samples in a set wave number range and with a silicon slice as a substrate; according to the Raman spectra of all the test samples, respectively using a successive projection algorithm and a multivariate linear regression analysis method to determine calibration wave numbers; according to the lead chrome green contents in the tea leaves corresponding to all the test samples, and ratio values of the peak intensities of all the calibration wave numbers to the 520 cm<-1> peak intensity, building a content-strength ratio first linear regression model as a calibration model, and measuring by using the calibration model to obtain the lead chrome green content in a to-be-measured sample. The detection method utilizes the Raman test for analysis, is simple to operate, does not need a tedious and time-consuming sample preparation process, at the same time, avoids interference from other sources so as to ensure the accuracy of the tested the tested lead chrome green, and greatly improves the test accuracy with silicon as a contrast.

The invention specifically discloses a diallyl phthalate colloidal gold immunochromatographic assay test strip which comprises a liquid absorption layer, a colloidal gold marking layer, a detection reaction layer, a water adsorption layer and a back liner. The diallyl phthalate colloidal gold immunochromatographic assay test strip has the advantages of being high in specificity, high in sensitivity, fast in detection speed, great in flux, simple and convenient to operate, stable in color, wide in detection temperature suitable range, long in expiration date of the test stripe, low in cost and the like, and semi-quantitative determination can be realized. The detection result of a colloidal gold kit is very close to a GC-MS detection result which is higher in precision. The result stability of different samples is relatively good, and the detection result of the kit and the actual result precision are controlled at about 97%, and the average deviation of three parallel detection results is controlled within 14%.

The invention discloses a method for detecting the concentration of light chrome yellow in a heavy metal concentrated alkali liquid. The method comprises the steps: taking heavy metal concentrated alkali liquids with different concentrations of light chrome yellow as test samples, and acquiring Raman spectra of all the test samples in a set wave number range and with a silicon slice as a substrate; according to the Raman spectra of all the test samples, respectively using a continuous projection algorithm and a multivariate linear regression analysis method to determine calibration wave numbers; according to the light chrome yellow concentrations of all the test samples, and ratio values of the peak intensities of all the calibration wave numbers in the corresponding Raman spectra to the 520 cm<-1> peak intensity, building a concentration-strength ratio linear regression model as a calibration model, and measuring by using the calibration model to obtain the concentration of light chrome yellow in a to-be-measured sample. The method utilizes the Raman test for analysis, is simple to operate, does not need a tedious and time-consuming sample preparation process, at the same time, avoids interference from other sources so as to ensure the accuracy of the tested light chrome yellow, and greatly improves the test accuracy with silicon as a contrast.

The invention discloses a cobalt ion test paper based on nanoscale zinc sulfide, a preparation method thereof and a test method. The preparation method for the cobalt ion test paper based on nanoscale zinc sulfide includes the following steps: (1) preparation of a zinc sulfide nanoparticle dispersant: zinc salt and sodium sulfide, the molar ratio of which is (0.25 to 4) to 1, are respectively dissolved in deionized water, so that a zinc salt solution and a sodium sulfide solution are obtained; the zinc salt solution is heated to 60 DEG C to 100 DEG C, and then added with mercaptoacetic acid dropwise, the mixture is stirred for 20 to 40 minutes, and the volume ratio of mercaptoacetic acid to the zinc salt solution is (0.01 to 0.1) to 1; the sodium sulfide solution is then added dropwise, and after 60 to 150 minutes of continued stirring, the zinc sulfide nanoparticle dispersant is obtained by cooling; (2) production of a test paper: an adsorption layer is immersed in the zinc sulfide nanoparticle dispersant obtained in Step (1) and sufficiently soaked, the soaked adsorption layer is then taken out and dried, and thereby the cobalt ion test paper is obtained.

The invention belongs to the field of advanced oxidation water pollution control, and relates to a micro-interface hydroxyl free radical characterization method based on fluorescence imaging analysis. According to the present invention, the HO. generation intensity on the micro-interface of a material is utilized by using a fluorescence microscopy imaging analysis technology; coumarin molecules rapidly act with HO. according to a dose ratio of 1:1 to convert into heptahydroxycoumarin with fluorescent luminescent property, and the research results of the Japanese team demonstrate that the concentration of the heptahydroxycoumarin is closely related to the coloration characteristic and the intensity of the fluoresce in a specific wavelength region; an excess amount of the coumarin is added to a heterogeneous reaction system as a HO. trapping agent, such that the intensity information of the HO. on the material surface layer can be effectively and indirectly acquired by using a fluorescence microscopy colorimetry method; and with the method, the semi-quantitative detection of the HO. at the solid-liquid micro-interface in the heterogeneous dynamic catalytic system can be achieved, the catalysis function of the function material can be directly identified and expressed in the imaging manner, and the method has characteristics of strong anti-interference and relatively simple operation steps .

The invention discloses a colorimetric sensing method for generating inorganic nanoparticles based on enzyme catalytic induction. The method comprises the steps: taking a frerric chloride solution, apotassium ferricyanide solution and an enzymatic solution, mixing, respectively adding the mixed solution into target solutions with known different concentrations, carrying out a reaction, quantitatively detecting by ultraviolet-visible spectroscopy to obtain the corresponding ultraviolet-visible absorbance values in all the concentrations, and thus obtaining a linear relationship between the target concentrations and the absorbance values; and adding a to-be-measured target solution to the mixed solution, detecting the absorbance value, and calculating to obtain the target concentration of the to-be-measured target solution. The method directly adopts Prussian blue as a colorimetric probe, and the constructed colorimetric sensing method has the advantages of being fast, simple, low in cost, high in sensitivity, good in selectivity and the like, has satisfactory results in application and has broad application prospects.

The invention relates to a tooth socket for detecting tooth diseases. The tooth socket comprises a tooth socket body. The tooth socket body is meshed with human oral cavity teeth. The tooth socket body can have a color development reaction with abnormal components in oral cavity gas. The tooth socket body is prepared by modifying an inert polymer material through an indicator and can have a colordevelopment reaction with abnormal components in the oral cavity gas, semi-quantitative detection can be realized for the health of the oral cavity teeth according to the content of the abnormal components in the gas, the detection cost is low, time is short, sensitivity is high, the affected teeth can be accurately located, and convenience is provided for further treatment of a patient.

The invention discloses a strip color developing type test strip for rapidly detecting the concentration of oxalic acid in urine as well as a preparation method, a use method and application of the strip color developing type test strip. The test strip is formed by overlapping four parts, namely a sample pad, a combination pad, a reaction film and an absorption pad. The method comprises the following steps: loading oxalate oxidase and horse radish peroxidase on the combination pad of the test strip; and a plurality of lines are uniformly scribed on the reaction film at intervals by using a color developing agent. The detection limit of the test strip is lower than 0.3 mM, and the detection range covers the concentration range of oxalic acid in urine from normal people to patients with hyperoxaluria. The method does not need image acquisition and processing equipment depended on by a traditional colorimetric method, realizes'equipment-free 'detection of oxalic acid, and has the advantages of simplicity and convenience in operation, low cost and capability of instantly detecting on site; the test strip can be prepared on a large scale, is widely applicable to household monitoring and bedside detection, and has a good application prospect.

The invention provides a batch calibration system and method for a rapid diagnosis test. The batch calibration system for the rapid diagnosis test comprises: a test strip batch information acquisition module, a batch calibration information acquisition module, a test data collection module, and a test data calibration module. Different batches of test bars can be calibrated by utilizing the batch calibration system for the rapid diagnosis test provided by the invention when being used by an actual user, so as to improve the accuracy of a test result.

The invention discloses a Sudan I immune chromatography test paper detection method. On the back liner of the test paper, a sample liquor absorption part, a colloidal gold marking part, a detecting reaction part and a water absorption part are sequentially pasted, wherein, the detecting reaction part is covered with one or two strips of Sudan I polyclonal antibodies or one or two strips of antigens used for detecting, which are used as detecting lines, meanwhile, one strip of IgG which resists the second species animal protein also covers the detecting reaction part and is used as a reference line. The combination mode has two ways, that is, one detecting line plus one reference line or two detecting lines plus one reference line. The fast detection test paper can be used when the temperature ranges from 4 DEG C to 40 DEG C and is suitable for fast detecting foods such as meat, etc. which may be polluted by Sudan I by departments such as sanitary, quality supervision, customs and supermarkets, etc. The Sudan I immune chromatography test paper detection method has the advantages of strong specificity, high sensitivity, capability for realizing semi-quantitative detection, simple and convenient operation, etc.

The invention relates to a sandwiched immunochromatographic test paper used for detecting Escherichia coli O157:H7. The sandwiched immunochromatographic test paper comprises a supporting layer, the upper side surface of the supporting layer is provided with adsorption layers, the adsorption layers sequentially comprise an adsorption fiber layer, a fluorescence antibody fiber layer, a cellulose film and a water absorbing material layer from a test end, the water absorbing material layer is arranged at a handle end, the cellulose film is provided with a detection line and a quality control line, the detection line is formed by an anti-Escherichia coli O157:H7 monoclonal antibody or polyclonal antibody, the quality control line is formed by a goat anti-mouse or rabbit anti-mouse IgG antibody, or a goat anti-rabbit IgG antibody, the fluorescence antibody fiber layer is adsorbed with a fluorescence antibody, and the fluorescence antibody is formed by an FITC labeled anti-Escherichia coli O157:H7 monoclonal antibody or polyclonal antibody. The invention also discloses a production method of the sandwiched immunochromatographic test paper. The test paper has the advantages of simple detection method, strong specificity, high sensitivity, visual property, high accuracy, and realization of lowest detection of 1CFU / mL of trace quantity pollution.

The invention discloses a method for detecting the concentration of lemon chrome yellow in a heavy metal concentrated acid liquid. The method comprises the steps: taking heavy metal concentrated acid liquids with different concentrations of lemon chrome yellow as test samples, and acquiring Raman spectra of all the test samples in a set wave number range and with a silicon slice as a substrate; according to the Raman spectra of all the test samples, respectively using a continuous projection algorithm and a multivariate linear regression analysis method to determine calibration wave numbers; according to the lemon chrome yellow concentrations of all the test samples, and ratio values of the peak intensities of all the calibration wave numbers to the 520 cm<-1> peak intensity, building a concentration-strength ratio first linear regression model as a calibration model, and measuring by using the calibration model to obtain the concentration of lemon chrome yellow in a to-be-measured sample. The method utilizes the Raman test for analysis, is simple to operate, does not need a tedious and time-consuming sample preparation process, at the same time, avoids interference from other sources so as to ensure the accuracy of the tested lemon chrome yellow, and greatly improves the test accuracy with silicon as a contrast.

The invention discloses escherichia coli O157:H7 direct type immunofluorescence chromatography test paper. The test paper comprises a supporting layer which is provided with an adsorption layer. A protective layer is arranged on the adsorption layer. The adsorption layer is composed of an adsorptive fiber layer, a cellulose membrane layer and a water absorption material layer, one end of the adsorptive fiber layer is closely connected with one end of the cellulose membrane layer, the other end of the cellulose membrane layer is closely connected with the water absorption material layer, and a detection line is arranged on the cellulose membrane layer and is composed of monoclonal antibodies or polyclonal antibodies composed of escherichia coli O157:H7. The invention further provides a method for preparing the chromatography test paper. The test paper and a detection method are simple, high in specificity, high in sensitivity, visual, accurate and capable of being used for performing qualitative and semi-quantitative detection, the trace contamination of the lowest 1CFU / mL can be detected, the application range is wide, cost is low, and the test paper and the detection method are easy to apply and popularize.

The invention discloses a target semi-quantitative detection method based on detection test paper and the detection test paper. The method comprises the following steps of covalently modifying a mixed solution of a probe I and a pH enzyme on silicon dioxide nano-particles through a covalent modification reagent to obtain immune silicon nanoparticles, incubating the paired probe II and the magnetic beads to obtain immunomagnetic beads, combining the immune silicon nanoparticles, the immunomagnetic beads and the target substance to form a three-layer structure of the immunomagnetic beads, the target substance and the immune silicon nanoparticles, carrying out catalytic reaction on the solution to be detected and a substrate of pH enzyme to change the pH of the solution to be detected, and dropwise adding the supernatant of the solution to be detected on the test paper, and obtaining a semi-quantitative detection result of the concentration of the target according to the color change and the standard curve of the concentration and pH. The detection test paper is adopted for rapid semi-quantification, meanwhile, the nano immunomagnetic bead separation technology and the immune silicon nanoparticle coupling adsorption technology are adopted, the method is suitable for rapid detection of multiple targets, sensitivity is high, universality is high, and the detection efficiency is greatly improved.

The invention discloses a method for detecting the concentration of lemon chrome yellow in a heavy metal concentrated acid liquid. In the method, heavy metal concentrated acid liquids with different concentrations of lemon chrome yellow are used as test samples, and each test sample is obtained within a set wave number range using a silicon wafer as the test sample. The Raman spectrum of the substrate, and according to the Raman spectra of all test samples, the continuous projection algorithm and the multiple linear regression analysis method were used to determine the calibration wavenumber, according to the lemon chrome yellow concentration of each test sample, and each calibration wavenumber The peak intensity and 520cm ‑1 The first linear regression model of the concentration-intensity ratio is constructed as the calibration model, and the concentration of lemon chrome yellow in the sample to be tested is measured by using the calibration model. The Raman test is used for analysis, which is easy to operate and does not require tedious and time-consuming sample preparation processes. At the same time, interference from other sources is avoided to ensure the accuracy of the test lemon chrome yellow, and the comparison with silicon greatly improves the test accuracy. accuracy.