The invention discloses a Sudan I immune
chromatography test paper detection method. On the back liner of the test paper, a sample liquor absorption part, a
colloidal gold marking part, a detecting reaction part and a water absorption part are sequentially pasted, wherein, the detecting reaction part is covered with one or two strips of Sudan I
polyclonal antibodies or one or two strips of antigens used for detecting, which are used as detecting lines, meanwhile, one strip of IgG which resists the second species
animal protein also covers the detecting reaction part and is used as a
reference line. The combination mode has two ways, that is, one detecting line plus one
reference line or two detecting lines plus one
reference line. The fast detection test paper can be used when the temperature ranges from 4 DEG C to 40 DEG C and is suitable for fast detecting foods such as meat, etc. which may be polluted by Sudan I by departments such as sanitary, quality supervision, customs and supermarkets, etc. The Sudan I immune
chromatography test paper detection method has the advantages of strong specificity, high sensitivity, capability for realizing semi-quantitative detection, simple and convenient operation, etc.