NTx (Naltrexone) detection test paper, kit and preparation method thereof

Abstract

The invention discloses an NTx (Naltrexone) detection test strip. The NTx detection test strip comprises a chromatography membrane and a conjugate pad; an immunolabelled NTx antibody and an immunolabelled first antibody are loaded on the conjugate pad; the chromatography membrane is provided with an NTx protein covered detection line and a second antibody covered colorimetric line along a liquid chromatography direction. When a sample is detected, the colorimetric line correspondingly displays the chroma of specific protein concentration after the second antibody is specifically combined withthe first antibody; NTx protein on the detection line and the NTx protein in the sample are competitively combined with the immunolabelled NTx antibody and the color developing depth of the detectionline and the colorimetric line is directly compared so that semi-quantitative detection of the NTx protein is realized. The invention discloses a preparation method of the NTx detection test strip; the preparation method is used for preparing an NTx protein semi-quantitative detection test strip which is rapid and sensitive in detection and does not depend on an instrument. The invention disclosesan NTx detection kit; the NTx detection kit comprises the NTx detection test strip and can provide effective information for early diagnosis and treatment of osteoporosis.

Description

technical field [0001] The invention belongs to the technical field of immunochemical detection, and in particular relates to an NTx detection test paper, a kit and a preparation method thereof. Background technique [0002] Osteoporosis (Osteoporosis, OP) is a systemic, systemic bone disease characterized by decreased bone mass and destruction of bone tissue microstructure leading to increased bone fragility and fracture risk. With the continuous increase of life expectancy and elderly population, the incidence of osteoporosis has jumped to the seventh place after cardiovascular and cerebrovascular diseases and cancer among the common frequently-occurring diseases in the world, and is the second place after cardiovascular diseases. Big medical problem. my country is the country with the largest number of osteoporosis patients, with an incidence of about 100 million people. Osteoporosis has become a major problem affecting the health of the elderly. About half of the women ...

AI Technical Summary

Problems solved by technology

[0008] Therefore, the technical problem to be solved by the present invention is to overcome the colloidal gold test strips for the detection of bone metabolism markers in the prior art that must rely on detection instruments to determine the detection results, resulting in high detection costs and complicated detection processes; thereby providing a detection cost Low cost, easy to operate, no need to rely on equipment, and a test strip that can complete the semi-quantitative detection of NTX1 through naked eye observation

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