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Target object semi-quantitative detection method based on detection test paper and detection test paper

A technology for semi-quantitative detection and test strips, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as single type and inability to detect multiple test objects, and achieve high accuracy and accurate preliminary semi-quantitative test results. , the effect of high versatility

Pending Publication Date: 2022-01-14
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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Problems solved by technology

[0002] On-site point-of-care testing (POCT, Point-of-care testing) is a method for immediate detection and analysis at the sampling site. It can well meet the needs of detection outside the laboratory; however, a POCT detector can detect a single type of item and cannot detect a variety of detection objects

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  • Target object semi-quantitative detection method based on detection test paper and detection test paper
  • Target object semi-quantitative detection method based on detection test paper and detection test paper
  • Target object semi-quantitative detection method based on detection test paper and detection test paper

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Embodiment

[0044] The invention provides a detection test paper, which is used to provide a fast, stable, accurate and effective universal semi-quantitative detection method for specific targets, and the targets can include ions, small molecules, nucleic acids, proteins, viruses, bacteria and cells and other specific targets, with a wide range of applications; and the detection test paper can drop liquid on it, and through the detection of the color change visible to the naked eye, the concentration range of the solution to be tested can be preliminarily determined to achieve rapid semi-quantitative detection; Instructions attached image 3 As shown, the detection test paper includes a substrate 1, a water-absorbing layer 2, a test paper layer 3 and a test paper shell 4, wherein the water-absorbing layer 2 is bonded to the upper surface of the substrate 1, and the test paper layer 3 is bonded to the upper surface of the water-absorbing layer 2 Finally, the test paper shell 4 covers the t...

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Abstract

The invention discloses a target semi-quantitative detection method based on detection test paper and the detection test paper. The method comprises the following steps of covalently modifying a mixed solution of a probe I and a pH enzyme on silicon dioxide nano-particles through a covalent modification reagent to obtain immune silicon nanoparticles, incubating the paired probe II and the magnetic beads to obtain immunomagnetic beads, combining the immune silicon nanoparticles, the immunomagnetic beads and the target substance to form a three-layer structure of the immunomagnetic beads, the target substance and the immune silicon nanoparticles, carrying out catalytic reaction on the solution to be detected and a substrate of pH enzyme to change the pH of the solution to be detected, and dropwise adding the supernatant of the solution to be detected on the test paper, and obtaining a semi-quantitative detection result of the concentration of the target according to the color change and the standard curve of the concentration and pH. The detection test paper is adopted for rapid semi-quantification, meanwhile, the nano immunomagnetic bead separation technology and the immune silicon nanoparticle coupling adsorption technology are adopted, the method is suitable for rapid detection of multiple targets, sensitivity is high, universality is high, and the detection efficiency is greatly improved.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a semi-quantitative detection method of a target based on detection test paper and the detection test paper. Background technique [0002] On-site point-of-care testing (POCT, Point-of-care testing) is a method for immediate detection and analysis at the sampling site. It can well meet the needs of detection outside the laboratory; however, a POCT detector can detect a single type of item, and cannot detect a variety of detection objects. [0003] Therefore, there is a need for a semi-quantitative detection method for targets based on detection test paper and detection test paper, which can be applied to a variety of target species, have strong versatility, good specificity, high sensitivity and accuracy, and contribute to the identification of targets in the biomedical field. rapid detection of substances. Contents of the invention [0004] Aiming at the problem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/01G01N33/52G01N33/543G01N33/552G01N33/58
CPCG01N21/78G01N21/01G01N33/587G01N33/552G01N33/54346G01N33/54326G01N33/52G01N2021/0112
Inventor 陈世兴李铁魏擅红王跃林
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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