191results about How to "Simplify testing procedures" patented technology

The invention belongs to the technical field of non-destructive testing and measuring, and discloses a focusing rotary scanning photoacoustic ultrasonic blood vessel endoscope imaging device and a focusing rotary scanning photoacoustic ultrasonic blood vessel endoscope imaging method. The device comprises a photoacoustic ultrasonic endoscope imaging probe, a rotating connecting part and a peripheral circuit part, wherein pulse laser generates 90-degree reflection at the light outlet end and is irradiated on the blood vessel wall after being gathered by a cylindrical surface light gathering lens, photoacoustic signals are generated, a sound-sensitive element receives the photoacoustic signals, the photoacoustic signals are collected and recorded after being converted, the synchronous working of a data collector and a pulse laser is realized, triggering signals generated by the pulse laser trigger an ultrasonic pulse emitting and receiving device to emit electric signals, the electric signals trigger the sound-sensitive element to emit ultrasonic signals, the ultrasonic signals are reflected after reaching the blood vessels, are received by sound-sensitive elements and are collected and recorded after being converted, a step motor drives the device for carrying out scanning to obtain the whole blood vessel fault data, and photoacoustic and ultrasonic images are obtained after the processing. The sound-sensitive elements are shared by ultrasonic and photoacoustic images, and the high-resolution and high-sensitivity blood vessel internal ultrasonic photoacoustic imaging can be realized.

The invention discloses an internal rectal optical, optoacoustic and ultrasonic multimode imaging endoscope which comprises a sleeve, an optoacoustic signal activating component, an ultrasonic signal activating and collecting component, an optical imaging component and an image reconstructing and displaying component. A device comprises a compact internal rectal optical, optoacoustic and ultrasonic multimode imaging endoscope, three kinds of imaging can be performed simultaneously, multi-parameter physical information and multi-scale structural images in recta can be acquired. The invention further provides a configuration scheme of the whole device and a method for utilizing the device for imaging. Three imaging techniques are highly integrated into one set of instrument, each imaging technique is optimized, and combination of three rectal endoscopic imaging methods of optical imaging, optoacoustic imaging and ultrasonic imaging is realized. The integrated endoscope combines specific advantages of three imaging modes, rectal physical images with multi-parameter information and multi-scale structural characteristics can be acquired, and application requirements of a rectal endoscope on medicine can be met better.

A method for holding samples for analysis and an apparatus thereof includes a testing plate with a pair of opposing surfaces and a plurality of holes. Each of the holes extends from one of the opposing surfaces to the other one of the opposing surfaces. The holes are arranged in groups, where each group has at least two rows and two columns of holes. The groups are arranged in sets, where each set has at least two rows and two columns of groups. To analyze samples, at least one of the opposing surfaces of the testing plate is immersed in a solution to be analyzed. A portion of the solution enters openings for each of the holes in the immersed opposing surface. Once the holes are filled with solution, the testing plate is removed and is held above a supporting surface. Surface tension holds the solution in each of the holes. The solution in one or more of the holes is then analyzed and the solution in one of these holes is identified for further study. The location of the identified solution is marked based upon its location within a particular set and group of holes.

The invention discloses an urban gas buried pipeline leakage detection and locating method and applications thereof. Based on an acoustic emission technology and combined with the current gas pipeline laying situation of Changzhou city, layout schemes and relevant parameters of sensors are designed, and the leakage detection method under the situation of guaranteeing normal gas transmission of buried pipelines and not excavating pipelines is determined. Simultaneously, wavelet noise reduction and relevant analysis methods are introduced, and accordingly, the locating accuracy is improved, and objective and credible results are obtained. According to the urban gas buried pipeline leakage detection and locating method and applications thereof, the application of the acoustic emission detection technology in gas buried pipeline leakage detection is facilitated and improved to a large extent, and hence, while the method is effective and accurate in detection and cost saving, popularization and use values are increased, and the method has great significances in enhancement of urban gas buried pipeline risk control.

The invention relates to an integrated opto-acoustic, ultrasonic and opto-acoustic elastic endoscopic imaging device which is characterized in that the imaging device comprises a pulse laser, an optical filter, a diaphragm, an objective and an optical fiber coupler sequentially arranged along the transmission path of pulse laser and also comprises a multimode optical fiber, an optical fiber slip ring, a rotation component, a rotary step motor, an electric slip ring, an integrated probe, a translation platform, a translation step motor, an ultrasonic pulse transceiver, a time delay unit and a computer, wherein the computer is in signal connection with the pulse laser; the pulse laser is in signal connection with the time delay unit; the time delay unit is in signal connection with the ultrasonic pulse transceiver; and the ultrasonic pulse transceiver is in signal connection with the electric slip ring. The invention also relates to a method for imaging by using the imaging device. In the invention, three kinds of imaging can be obtained, the detection precision can be improved while multi-parameter and multi-scale information in blood vessels can be obtained, and the invention belongs to the technical field of blood vessel endoscopy.

The present invention relates to a colloidal gold semiquantitative immunologic diagnosis test paper preparing and assembly method. Said test paper utilizes immunity chromatograph principle capable of rapid visual inspection nano colloidal gold marker and sample combined colour, judging detective semiquantitative result through test zone with parallel reference zone colour contrast.

The invention provides a preparation method of urea detection test paper in dairy and a detection method of urea; urease source powder is taken and mixed with pH indicator with the pKHIn more than 6.8; the mixture is diluted by deioned water and the filter solution or the upper suspension liquid after natural deposition is taken, uniformly fixed on a carrier with good water absorption by the forms of coating, printing or soaking, and dried subsequently, thus forming the urea detection test paper; the detection method of the dairy comprises the steps as follows: firstly, common pH test paper is used for detecting the dairy to be detected; whether the dairy to be detected is in normal pH range 6.3-6.8 or not is checked; if so, the urea detection is directly carried out; if not, the pH value is adjusted to the normal pH range and the urea detection is carried out subsequently; one piece of urea detection test paper dips the dairy to be detected and the color change of the test paper is observed; if the color of the test paper is not changed, the dairy is qualified; if the color of the test paper changes, the dairy is mixed with the urea, thus carrying out qualitative judgment on the dairy; and the displayed color is compared with a standard colorimetric card, so as to carry out semi-quantitative detection of the urea in the dairy.

The invention discloses a kit for detecting klebsiella pneumoniae, and belongs to the technical field of PCR detection. The kit comprises specific primers and a probe for detecting klebsiella pneumoniae; the nucleotide sequences of the specific primers and the probe are as shown in SEQ ID NO.1-3; a detected target gene is a sequence of a pho gene, and has a nucleotide sequence as shown in SEQ ID No.4. The kit has the advantages of being accurate in detection, high in sensitivity, strong in specificity, and simple and quick to operate, has a favorable sample detection capacity, can replace a traditional bacterial isolated cultivation and diagnosis method, and the novel kit for quick detection is provided for klebsiella pneumoniae.

The invention belongs to the field of intravascular endoscope imaging and discloses an intravascular fluorescent-photoacoustic-ultrasonic multi-mode imaging device and method. The device comprises a computer, a fluorescence excitation and acquisition system, a photoacoustic signal excitation and acquisition system, an ultrasonic signal excitation and acquisition system and an integrated probe. The fluorescence excitation and acquisition system comprises a pulsed laser, a first optical filter, a collimator, a first optical fiber coupler, an optical fiber circulator, a photoelectric sliding ring, a second optical fiber coupler, a second optical filter, a photoelectric multiplier tube, a first signal amplifier and a first acquisition card. An intravascular endoscope imaging system in a fluorescent imaging mode, an intravascular endoscope imaging system in a photoacoustic imaging mode and an intravascular endoscope imaging system in an ultrasonic imaging mode are integrated, and due to combination of the advantages of the three imaging modes, structural information of multiple parameters of vessels and component information of corresponding substances can be obtained.

The invention discloses an ISSR molecular marking based method for constructing a Chinese asparagus bean generic resource database and application thereof. The invention also utilizes the data base to identify the trueness of a variety; according to field forms, the consistency and typicality among individual plants of the variety are identified, separated and atypical materials are given up, and DNA of the individual plant of the consistent and typical variety is picked up; by utilizing three ISSR markings with high polymorphism, the consistency of DNA level among the individual plants is primarily selected, and the variety and individual plant constructing a fingerprint are finally determined; and by utilizing the finally selected variety and individual plant, the ISSR analysis of the Chinese asparagus bean is made to construct a fingerprint database. The invention overcomes the defects existing in the construction of the prior generic resource database, such as separation among different individual plants of the variety and impurity on the DNA level, the reliability and accuracy of the Chinese asparagus bean ISSR fingerprint data base constructed by the method are greatly improved, and the planting varieties of the Chinese asparagus bean can be effectively and conveniently identified and distinguished.

The invention discloses a design way of primers for in-vitro qualitative detection of newborn inherited metabolic disease genes. The design way comprises the following steps: (1) acquiring relevant disease-causing gene information of inherited diseases: acquiring the relevant disease-causing gene information, including gene location information and mutation site information of a plurality of inherited diseases through an OMIM (Online Mendelian Inheritance in Man) database, and acquiring exon and intron information of relevant genes from an NCBI (National Center for Biotechnology Information) database according to the obtained gene information; (2) designing primers specific to the relevant genes: designing corresponding specific primers F1 and R1 specific to an exon and an exon-intron combination area of each gene. The invention also discloses the first-round PCR (Polymerase Chain Reaction) primer sequence list of syndromic deafness, a first-round PCR primer list of G6PD genes, and a first-round PCR primer sequence list of SLC12A3 genes. The primers can be used for detecting relevant genes of 22 types of common newborn inherited diseases.

The invention relates to a method for quickly, qualitatively and quantitatively measuring Lactobacillus plantarum in probiotic dairy products. The method comprises the following steps: aiming at the lactobacillus plantarum in probiotic dairy products, independently designing a species specificity primer of the 16S rRNA gene sequence of the lactobacillus plantarum, carrying out species specificity PCR and real-time fluorescent quantitative PCR reaction by using the primer, and analyzing through an agarose gel electrophoresis pattern and a real-time fluorescent quantitative PCR pattern, thereby establishing the method for simply, conveniently, quickly, accurately, qualitatively and quantitatively measuring lactobacillus plantarum in probiotic dairy products.

The invention discloses a preparation method and an application method of a nucleic acid rapid detection kit for Bostaurus components in feeds and belongs to the field of molecular biology and immunology. According to the preparation method and the application method, a high-sensitivity and high-specificity method of a polymerase chain reaction in nucleic acid detection is combined with an immuno gold staining rapid detection technology in immunology detection; a unique primer is designed and the primer is labeled; an extracted target DNA is subjected to specific amplification and an amplified product is combined with a labeled antibody immobilized on a test strip in a developing solution to form a stable and visible detection strip and a quality control strip, so as to realize rapid and accurate detection on the bovine-derived components in food and feed.

The invention relates to a detection method of degree of damage of plasma, which comprises the following steps: providing a batch of chips; providing the upper limit value of the threshold voltage shift of the batch of chips; testing the drain current of the batch of chips to obtain the distribution of the drain current; extracting part of chips from the batch of chips, testing the threshold voltage of the part of chips to obtain the threshold voltage shift and distribution thereof; determining the qualification rate of the part of chips according to the threshold voltage shift and the upper limit value; applying the qualification rate of the part of chips to the batch of chips to establish the drain current standard value of the batch of chips; and detecting the degree of damage of plasma of the batch of chips according to the drain current standard value of the batch of chips. The method can lower the detection cost, enhance the detection efficiency and realize on-line measurement without damaging the chips.

The invention discloses a preparation method of a rapid nucleic acid detection kit for detecting canis familiaris components in feed and an application method thereof, and belongs to the fields of molecular biology and immunology. According to the invention, a high sensitivity and high specificity method of polymerase chain reaction in nucleic acid detection and an immuno gold staining rapid detection technology in an immunological detection are combined, a specific primer is designed and labeled, the extracted target DNA is subjected to specific amplification, and amplified product is combined with a gold labeled antibody immobilized on a test paper tape, so as to form a stable and visible detection band and quality control band, thereby achieving the rapid and correct detection of the canis familiaris components in the main feed.

The invention discloses a liberobacter asiaticum detection method based on visible light images. In the training stage, a large number of images of citrus leaves infected with liberobacter asiaticum and images of normal leaves are collected, the feature values of the texture feature and the color feature are extracted, the above feature values and the feature values of normal leaves are trained and learned through a BP neural network, and the optimal BP neural network model is obtained; in the identification stage, the features of the leaf images to be identified are extracted and input into the optimal BP neural network model, and then whether a citrus tree is healthy is judged. By means of the liberobacter asiaticum detection method based on visible light images, the type of the liberobacter asiaticum and whether the citrus tree is not yellowed due to liberobacter asiaticum are further judged, early, accurate and non-destructive diagnosis can be carried out on the liberobacter asiaticum, and the liberobacter asiaticum detection method based on visible light images has the advantage of being high in detection accuracy.

The invention relates to a method for quickly, qualitatively and quantitatively measuring Lactobacillus casei in probiotic dairy products. The method comprises the following steps: aiming at the Lactobacillus casei in probiotic dairy products, independently designing a species specificity primer of the 16S rRNA gene sequence of the Lactobacillus casei, carrying out species specificity PCR and real-time fluorescent quantitative PCR reaction by using the primer, and analyzing through an agarose gel electrophoresis pattern and a real-time fluorescent quantitative PCR pattern, thereby establishing the method for simply, conveniently, quickly, accurately, qualitatively and quantitatively measuring Lactobacillus casei in probiotic dairy products.

The invention relates to a full-automatic detection system based on a microscope, which comprises a mechanical system arranged on a base, an optical microscope, a control system for controlling a stepper motor of the mechanical system, a CCD camera arranged on the optical microscope rack and a computer located outside the base, wherein the mechanical system moves a detected smear under the optical microscope, light emitted by the light source of the optical microscope passes through the detected smear, focused imaging on an electronic eyepiece of the CCD camera is realized through an objective lens, and the CCD camera converts optical signals for imaging into electric signals and transmits the electric signals to the computer for image processing and detection. Full-automatic detection and recognition on the detected object can be realized, the automatic degree is high, the detection process is greatly simplified, errors brought by subjectivity of manual detection and insufficient experience are reduced, the detection speed and the efficiency are improved, the manual labor intensity is reduced, and human resources are saved.

The invention provides a target gene and a detection kit used for detecting depressive disorder. The target gene used for detecting the depressive disorder is ATP1A1, and nucleotide sequences of a real-time fluorescence quantification PCR (polymerase chain reaction) primer for detecting the gene are shown as SEQ ID NO.2-3. The invention also provides the detection kit used for quantitative detection on ATP1A1. Effective guidance is provided for clinic detection of the depressive disorder by application of the ATP1A1 in preparation of medicines for diagnosing, treating and preventing the depressive disorder.

The invention belongs to the detection technical fields of feed science and feed additives, and more specifically relates to a rapid qualification and quantitation measurement method of saccharomyces cerevisiae in an additive premix sample. According the invention, cDNA gradient dilution is taken as an external standard substance used for distinguishing dead bacteria and live bacteria for accurately detecting the amount of the live bacteria in a sample to be measured. The invention is capable of measuring saccharomyces cerevisiae in premix sample with simple, rapid and accurate qualification and quantitation without pure culture of saacharomyces cereisiae, the whole process has the advantages of simple detection process, high detection efficiency, high accuracy and short detection period; and the invention provides a guarantee a long term development for a microecological preparation industry.

The invention discloses a visual inspection method for coated paper. The visual inspection method comprises the following steps that a standard image is registered; a camera is used for carrying out primary shooting imaging on the inner wall of a zinc barrel of a product to be inspected within the view field of the camera, a visual controller controls a first camera to carry out primary shooting imaging on the bottom of the zinc barrel of the product to be inspected, and the image is marked as P1; the similarity of the image of the product to be inspected and the standard image is calculated; if the similarity of the image of the product to be inspected and the standard image is lower than a set value, it is judged that the product to be inspected is a defective product; if the similarity is larger than the set value, position displacement correction is carried out on the obtained image of the product to be inspected through the visual controller, so that the center of the image of the product to be inspected is overlapped with the center of the standard image; area gravity center detection is carried out, and the defects that no coated paper exists, the coated paper is too low or too high, and no bowl bottom paper exists are detected. By means of the method, the problems that current manual visual inspection is low in efficiency, poor in inspection effect and different in visual inspection standard, and consequently product stability is poor can be effectively solved.

The invention discloses a reagent kit for detecting chromosome number of fetus and a method for detecting chromosome number of fetus that belong to the field of chromosome detection. The reagent kit comprises a reagent I that modifies DNA, phosphorylated sequencing linkers for linking two terminals of the modified DNA, a reagent II for linking the DNA phosphorylated sequencing linkers together, and a device for directly purifying the ligation product without amplifying by a primer. The provided method for detecting chromosome number of fetus comprises four steps, including a DNA extraction step, a DNA terminal modification step, a sequencing linker connection step, and a purification step. A sequencing library can be acquired without a PCR amplification. The disclosed reagent kit and the prepared library are able to achieve an efficient, precise and non-invasive effect for the detection of chromosome number of fetus.

The invention discloses a primer and a kit for detecting microdeletion of a chromosome Y and a use method of the primer. The primer disclosed by the invention is a specific primer aiming at six STSs (Sequence Tagged Sites) and reference genes, and the kit containing the primer can be used for rapidly and accurately detecting the microdeletion of the chromosome Y through the combination of multiplex PCR (Polymerase Chain Reaction) amplification and DHPLC (Denaturing High Performance Liquid Chromatography) detection, so that detection program is simple and the detection sensitivity, the result repeatability and the sample flux are high.

The invention relates to a portable photovoltaic subassembly power testing instrument and a testing method thereof, in particular to a hand-hold portable photovoltaic subassembly power testing instrument and a testing method thereof applied under the nonstandard environment. The power testing instrument comprises a standard photovoltaic subassembly, a port, current sensors used for measuring short circuit current of the standard photovoltaic subassembly and the short circuit current of a tested photovoltaic subassembly, and a microprocessor used for calculating the standard power of the tested photovoltaic subassembly. The testing method is on the basis of a formula that 'standard power of the tested photovoltaic subassembly is equal to standard power of the standard photovoltaic subassembly* open-circuit voltage of the tested photovoltaic subassembly* short circuit current of the tested photovoltaic subassembly / open-circuit voltage of the standard photovoltaic subassembly* short circuit current of the standard photovoltaic subassembly'. According to the portable photovoltaic subassembly power testing instrument and the testing method provided by the invention, the standard power can be obtained without debugging when the environment is varied; and the portable photovoltaic subassembly power testing instrument and the testing method can be applied to the indoor and outdoor, therefore, the detecting process of a photovoltaic subassembly can be simplified, and the efficiency can be maximized, and the problem that the power test is carried out with low efficiency and high cost caused by adopting a solar simulator for a long time can be completely solved.

The invention relates to a semiconductor test management system, comprising a circuit probe detection map recording the electricity property of a wafer; an automatic defect detection device detecting the defects of the wafer and a computer storing selection software, wherein the selection software contains selection standards for selecting the defects of the wafer. The semiconductor test management can detect the defects of wafers, simplifies test program and adopts computer to improve test efficiency.

The invention provides a RPA (recombinase polymerase amplification) kit used for detecting Ebola virus, special-purpose primers and probes of the RPA kit, and applications of the RPA kit in detection of Ebola virus. The RPA kit, the special-purpose primers, and the probes are designed based on Ebola virus NP gene conserved sequence, and the Ebola virus NP gene conserved sequence possesses an oligonucleotide sequence represented by SEQ ID NO.1. It is shown by research results that the RPA kit can be used for rapid detecting of Ebola virus in samples with specificity and sensitivity.

The invention belongs to the technical field of bioengineering, and in particular relates to a method for quantitatively analyzing clostridia, lactobacilli, bacilli and methanobacteria in pit mud. The technical problem to be solved by the invention is to provide a new choice for analyzing the clostridia, lactobacilli, bacilli and methanobacteria in the pit mud. The technical scheme of the invention is as follows: the method for quantitatively analyzing the clostridia, lactobacilli, bacilli and methanobacteria in the pit mud comprises the steps of a, preparation of a standard curve; b, extraction of genome DNA; c, amplification; d, quantitative analysis. The method disclosed by the invention can be used for analyzing the change trend of the clostridia, lactobacilli, bacilli and methanobacteria in the pit mud.

The invention provides a genetic marker for detecting canine parvovirus, and the genetic marker contains a nucleotide sequence as shown in SEQ ID NO.1. The invention also provides specific primers and a probe for detecting the genetic marker, a method for quantitative detection on the canine parvovirus and a detection kit. The method and the kit, provided by the invention, have the advantages of high sensitivity, strong specificity and easiness in operation, can meet the requirement of rapid detection on sick animals or products thereof, and has the significance for guaranteeing healthy development of the domestic animal husbandry and preventing the canine parvovirus from spreading, and meanwhile provides a new technological means for study on pathogenicity of the canine parvovirus in a natural reservoir.

A testing device for testing computer peripheral devices with Universal Serial Bus (USB) is provided. The testing device includes a CPU, a memory with a predetermined firmware program inside, which is connected to the CPU, a control chip with USB interface, which is connected to the CPU and is provided with a plurality of connecting ports for connecting with peripheral devices to be tested. The firmware program inside the memory is used for controlling the CPU to test the peripheral devices to be tested through the control chipset. According to one CPU and each peripheral device connected through by a control chip with USB interface for processing test procedures only, the hardware could be more simpler and efficiency.

The invention relates to a method for detecting ractopamine content in food. The method is characterized by comprising the following steps: preparing a capture probe; preparing a signal probe, treating a sample to be detected, and detecting by using a glucometer. Compared with the prior art, the method has the advantages that the signal is converted into a glucose signal which can be detected by the glucometer through an enzymatic effect, field detection of a nonsugar glucometer is realized, the stability is high, the detection limit is low, the detection range is wide. Moreover, the detection cost is low, the detection program is simple, the detection effect is good, particularly general staff can perform field detection, and the strict requirement that the professional staff need to operate in a lab in the prior art is avoided.