The invention relates to a sample pretreatment integrated
chip which comprises a cover plate and at least one layer of structure plate, wherein the structure plate is provided with micro grooves and at least three holes communicated with the micro groove; and the micro grooves are at least two of the following grooves: a separation groove having a
micro structure of cross-flow separation or cross-flow
filtration, a purifying groove having a
solid phase extraction
micro structure and a mixed groove formed by converging at least two secondary grooves. The sample pretreatment integrated
chip has the pretreatment functions of separation,
filtration, mixing, purifying, and the like, is used for the pretreatment of samples of blood,
urine, and the like and gains analysis samples of
DNA,
protein,
amino acid, and the like which satisfy the follow-up detection need. The sample pretreatment integrated
chip separates or filters granules or cells on the basis of the principle of cross-flow separation or cross-flow
filtration, ruptures cells on the basis of the principle of a chemical method and purifies target molecules on the basis of the principle of
solid phase extraction. The invention develops the integrated micro-fluidic chip for the pretreatment of multiple samples by adopting the micro-electromechanical
system technology, has at least two sample pretreatment functions and has continuous and efficient sample pretreatment effect.