366 results about "Diseased plant" patented technology

A portable fluorescence and transmittance imaging spectroscopy system for use in diagnosing plant health. The system has a primary LED light source array with spectral wavelengths in the 400-600 nm range, a focus cone that collects the LED light source output and focuses it, a controller that controls the primary LED array to turn it on and off, or certain of the spectral wavelengths on and off such that the primary LED array controllably emits light of a desired wavelength in the range, the light irradiating the plant through the focus cone, a digital imaging device that both spatially and temporally captures a fluorescence image comprising chlorophyll fluorescence emitted by the plant due to the emitted light from the LED array, a leaf holder located proximate to the output of the focus cone to maintain a consistent position and distance between the digital imaging device, the LED light source and the leaf and providing for fixed position and non-destructive leaf imaging and testing, a secondary light source for providing broad-band transmissive light through the leaf, a lens for focusing onto the imaging device the light emitted from the secondary light source, and one or more memory devices that store the fluorescence image and the transmitted light data received by the digital imaging device and store a library of plant fluorescence-intensity data indicative of both healthy plants and stressed or diseased plants, and plant light transmittance data indicative of certain plant conditions.

A pest and disease identification method and device provided by the invention are applied to the data processing equipment, wherein the data processing equipment is pre-configured with a trained neural network model, and the neural network model is obtained by training leaf images and plant images of a plurality of diseased plants. The data processing equipment obtains a leaf image and a plant image of a to-be-identified plant, identifies the characteristics of the to-be-identified plant through the neural network model, and further obtains the type of the to-be-identified plant and the pest and disease damage type. Therefore, by combining the leaf image of the plant with the plant image of the plant, the identification rate of the plant species and the identification rate of the disease types of different plants are improved.

The invention discloses a chlorophyll fluorescence and imaging technology based plant disease detection method and a detection device. The detection device is arranged in an illumination box, a blue LED (light emitting diode) lamp is used as an excitation light source, an equilaterally triangular structure is formed, can achieve stable and uniform illumination and is used for exciting chlorophyll fluorescence of plant leaves, and a colored high-speed camera and a front red optical filter with adjustable lens are adopted for filtering interference light and collecting a chlorophyll fluorescence image; the leaves and the background are separated and a sub-image of a pixel region taking the main leaf vein as the center position is obtained through the steps of image pretreatment, image division and characteristic extraction, the vein characteristics and the leaf vein characteristic parameters of the leaves are calculated, and finally, the plants are divided into healthy plants and disease plants through computation by a classifier; the method and the device can be used for exciting the chlorophyll fluorescence of the plant leaves, acquiring the chlorophyll fluorescence image of the plant leaves, calculating the vein characteristics and the leaf vein characteristic parameters and detecting the plant diseases.

The invention provides a CNN and transfer learning-based disease intelligent identification method and system, which can reduce interference of a picture background, can achieve high recognition accuracy under the condition of a limited sample number, and supports higher training sample multi-classification operation efficiency. The disease image identification method comprises the following steps: image preprocessing: normalizing the size of the image, quickly positioning a disease region by using Fast-RCNN, and eliminating background interference; image feature extraction: using a triplet similarity measurement model to extract image features, and then using SIFT features as compensation features to perform weighted fusion; and disease classification and recognition: learning a first image feature of a normal plant image by adopting a deep convolutional neural network, then learning a second image feature of the disease plant image by using transfer learning, and finally performing classification and recognition by combining the first image feature and the second image feature.

The invention relates to the technical field of traditional Chinese herbal medicine cultivation, in particular to a herbahouttuyniae cultivation method, which comprises the following steps of: 1) determining a planting time: planting seedlings at a period from the middle ten days and last ten days of September to the first ten days of November every year; 2) determining a planting mode: selecting high-stalk crops for interplanting; 3) preparing the seedlings: conducting asexual reproduction by using subterraneous stems of herbahouttuyniae as reproduction materials; 4) planting the seedlings: directly placing the seedlings on compartment surfaces and covering the compartment surfaces with rice straw or maize stalks after the seedlings are planted; 5) conducting field management: removing weed at the growth period of the herbahouttuyniae, removing ill herbahouttuyniae plants, weak herbahouttuyniae plants and non-herbahouttuyniae crops during weeding, and making sure that the herbahouttuyniae is not damaged; and during top dressing, using farm manure as main fertilizers and chemical fertilizers as auxiliary fertilizers, paying attention to reasonable drainage and irrigation and keeping soil wet at the entire growth period of the herbahouttuyniae; and 6) harvesting at a proper time and the like. By adopting the herbahouttuyniae cultivation method, the yield of the herbahouttuyniae is high, the damage by diseases and insects is reduced, the herbahouttuyniae diseases such as southern blight and zonate spot can be effectively prevented and controlled, and the yield of the herbahouttuyniae and the mass content of medicinal components in the herbahouttuyniae are improved to the utmost extent.

A potato planting method comprises the following steps of (a), preparation before planting, (b), planting, (c), field management and (d), harvesting. By using the planting method, planted new Daping level potatoes are high in yield and high in disease resistance, and the shortcoming that the new Daping level potatoes planted by using the traditional planting mode are not high in yield, late blight resistance is not high and the like is overcome. Compared with the traditional cultivation technology, the potato planting method has the advantages that the yield and the quality of potatoes are greatly improved, the yield is improved by 15%, the dry matter content is increased by 12.53%, the reducing sugar content is increased by 20.00%, the soluble protein content is increased by 6.76%, the starch content is increased by 19.07%, and the Vc content is increased by 18.39%; and in 3-5 days before the late blight center diseased or infected plants of the potatoes are found out, the blight of the potatoes can be effectively prevented and cured by using bactericide, and the disease rate is reduced by 27.89%.

The invention discloses a method for obtaining sogatella furcifera carrying SRBSDV (southern rice black-streaked dwarf virus) and an application sogatella furcifera. The method for obtaining the sogatella furcifera carrying the SRBSDV, comprises the following steps of: feeding the sogatella furcifera by utilizing rice infecting the SRBSDV or cryopreserved rice diseased plant so that the sogatella furcifera obtains the SRBSDV, transferring the infected sogatella furcifera to a healthy rice to be fed till passing through a circulation period of virus, and detecting the sogatella furcifera by utilizing an RT-PCR (reverse transcription-polymerase chain reaction) technology and a serology method to obtain the sogatella furcifera carrying the SRBSDV, wherein the carrier rate of the sogatella furcifera can reach more than 50% through a manual feeding manner based on the experiment. Through utilizing the method for obtaining the sogatella furcifera carrying the SRBSDV, the obtained sogatella furcifera carrying the SRBSDV can be applied to screening of SRBSDV-resisting monoclonal antibody to carry out a rice inoculation experiment to identify the resistance of the rice, screen disease-resistant variety and provide a service of the breeding for disease resistance. The invention can be further applied to researching mutual relation between the SRBSDV and a vector insect sogatella furcifera and provides powerful theoretical evidence for prevention and treatment on the viruses.

The invention provides a prevention and control method of potato late blight, which is used for preventing and controlling potato late blight by comprehensive utilization of the traditional measures. The prevention and control method comprises the aspects of: potato seed treatment before sowing, management at seedling stage, cultivation measures, prediction and pre-warning, chemical control and the like. The prevention and control method is stable and permanent in prevention and control, reduces environmental pollution, is low in cost, and can predict and persistently control, thereby being amethod for realizing comprehensive prevention and control of potato late blight. From the growing conditions of the above-ground parts in field, the purpose of controlling the incidence and prevalence of late blight is achieved by adopting a late blight prediction and pre-warning system to instruct the chemical control on the field late blight. By adopting the prevention and control method, the disease plants are few, the yield is increased and is 25.7-45.8% higher than that of common planting, and the chemical spraying frequency is simultaneously reduced.

The invention relates to a single strand conformation polymorphism (SSCP) marker closely linked with a major wheat scab resistance quantitative trait locus (QTL). The SSCP marker is characterized in that: scab resistance candidate genes of a scab resistance QTL in a 3BS area of a wheat variety or strain resisting scab is PCR amplified by using a primer; after denaturalization, a PCR-amplified product has different single strand conformation; and the SSCP marker closely linked with the major wheat scab resistance QTL is established for detecting a genotype of the wheat variety or strain during breeding. The SSCP marker has the advantages of: overcoming the disadvantages that the wheat scab resistance screening can only be authenticated in a flowering period and is easily influenced by the environment in conventional breeding, predicting and screening wheat plants with the scab resistance by detecting a molecular marker at a seedling stage, eliminating disease plants, reducing waste of labor and materials and improving the breeding efficiency. Compared with an ABI DNA sequence analysis meter-based marker, the SSCP marker closely linked with the major wheat scab resistance QTL has the advantages of simple and convenient operation, low cost and same sensitivity.

The invention belongs to the plant protection subject of the agricultural category, and particularly relates to citrus yellow shoot candidatus liberibacter asiaticus detection test paper. The detection test paper is prepared through the following steps that pathogenic bacteria antigen is extracted and purifed from catharanthus roseus host with citrus yellow shoot candidatus liberibacter asiaticus antigen and used for injecting immunized rabbits to obtain yellow shoot candidatus liberibacter asiaticus specific antiserum, the yellow shoot candidatus liberibacter asiaticus specific antiserum is purified to obtain the citrus yellow shoot candidatus liberibacter asiaticus antigen, immune colloidal gold is prepared, a combined pad which is soaked with the immune colloidal gold and a chromatography membrane which is coated with a detection line T-citrus yellow shoot candidatus liberibacter asiaticus antigen and a quality control line C-goat anti-rabbit antibody are further prepared, and finally a detection test paper product is assembled. Against for the requirement of the detection of a great amount of citrus yellow shoot samples, the citrus yellow shoot candidatus liberibacter asiaticus detection test paper which is convenient, rapid and high in specificity is provided. The test paper has following advantages that 1, convenience in use and simpleness in operation are realized; 2, a detection result can be obtained within a short period of time; 3, the stability is good; and 4, the cost is low, and the test paper is particularly suitable for the discrimination of citrus yellow shoot plants of a fruit garden.

The invention provides a method for laodelphax striatellus to obtain a rice black-streaked dwarf virus (RBSDV) from refrigerated diseased leaves. The principle of the invention is as follows: storing plant leaves carrying RBSDV by using a refrigerated method, obtaining RBSDV for laodelphax striatellus from refrigerated diseased leaves by a feeding method, and obtaining laodelphax striatellus with RBSDV applied in inoculating and identification after laodelphax striatellus passes a circulative period of virus. The varietalresistance identification method established by the invention can overcome the technical bottleneck of difficult long-term storage of diseased plants by adopting the method of obtaining the virus which feeds laodelphax striatellus with diseased plant better, broadens the varietalresistance identification time, and is hopeful for quickening the screening speed of disease-resistant resources and breeding process of disease-resistant varieties.

The invention relates to a loop-mediated isothermal amplification (LAMP) detection method for the specificity of radopholus similis and application thereof, and belongs to the technical field of biology. In the LAMP method for the specificity of the radopholus similis, LAMP detection primers for the specificity of the radopholus similis in a reaction system are RSF3, RSB3, RSFIP, RSBIP and RSLF respectively, and the radopholus similis is detected quickly and specifically by extracting the DNA of the radopholus similis or single-head radopholus similis in diseased plant tissues and performing LAMP isothermal amplification. Therefore, the detection method is high in specificity and sensitivity and low in cost, is easy and convenient to operate, and has the high application value in aspects of the on-site quick detection of the radopholus similis and the early diagnosis of radopholus similis diseases.

This invention relates to Bacillus subtilis and its application. Bacillus subtilis Jaas ed1 is stored in China General Microbiological Culture Collection Center on Dec. 12, 2005 with a number of CGMCC No.1564. Bacillus subtilis Jaas ed1 can enter crops with a low bacteria solution concentration, and colonize in crops. Bacillus subtilis Jaas ed1 has good effect on preventing plant diseases, especially soil-borne diseases. Besides, Bacillus subtilis Jaas ed1 can promote plant growth and produce various antibacterial metabolites, and has such advantages as low cost, easy operation, no pollution and no harm.

Relating to the technical field of virus detection in genetic engineering, the invention provides a method for rapid and accurate detection of tomato yellow leaf curl virus (TYLCV). The method comprises: cloning a TYLCV-CP gene segment from the total DNA of a diseased leaf, connecting the TYLCV-CP gene segment with pET-32a and transforming a recipient bacterium, conducting inducible expression to a positive bacterial colony and purifying the target protein; preparing antiserum with an immunized rabbit, labeling a purified antibody with alkaline phosphatase, and detecting a diseased plant to be detected by a DAS-ELISA (double antibody sandwich-enzyme-linked immunosorbent assay) method. The method for detection of TYLCV in the invention has high sensitivity and can be used for qualitative and quantitative analysis, with a lowest detection limit of 9.75ng / mL; it also has simple and rapid operation that can be finished within several hours; with strong specificity and good repeatability, the method has very obvious immunological reaction to TYLCV; characterized by high reliability, the detection accuracy of the method is very close to PCR (polymerase chain reaction) method; the detection cost is low, and the reagent has small consumption amount and can be stored for a long time. Thus, the method provided in the invention is very suitable for large scale routine detection.

The invention discloses a potato breeder's seed seedling-raising substrate and a preparation method thereof, and belongs to the field of potato breeding and planting. The substrate comprises 20-40 parts of loam compound, 5 parts of potash fertilizer and 65-85 parts of specially-made organic fertilizer, wherein the loam compound comprises loam and a recycled substrate, the specially-made organic fertilizer is prepared by fermenting fallen leaves, manure, potato stems and leaves, coal ash powder and rice bran through microorganisms, and the fallen leaves include 60-80% of pine needles. The substrate mainly comprises the loam, the recycle substrate and the specially-made organism fertilizer. The specially-made organic fertilizer mainly comprises the fallen leaf pine needles, the manure and the potato stems and leaves free of diseased plants. The loam and the recycled substrate are exposed in sun and dried at high temperature, and the specially-made organic fertilizer is fermented at high temperature, so most bacteria, insect eggs and seeds can be killed, and convenient, clean and fertile substrate soil is provided for potato breeder's seed seedling-raising. The component raw materials of the substrate are wide in source, waste is reused due to the recycled substrate and the potato stems and leaves produced in seedling raising, and the substrate is quite suitable for being used and popularized in potato seedling raising bases in suburbs and countries.

A portable fluorescence and transmittance imaging spectroscopy system for use in diagnosing plant health. The system has a primary LED light source array with spectral wavelengths in the 400-600 nm range, a focus cone that collects the LED light source output and focuses it, a controller that controls the primary LED array to turn it on and off, or certain of the spectral wavelengths on and off such that the primary LED array controllably emits light of a desired wavelength in the range, the light irradiating the plant through the focus cone, a digital imaging device that both spatially and temporally captures a fluorescence image comprising chlorophyll fluorescence emitted by the plant due to the emitted light from the LED array, a leaf holder located proximate to the output of the focus cone to maintain a consistent position and distance between the digital imaging device, the LED light source and the leaf and providing for fixed position and non-destructive leaf imaging and testing, a secondary light source for providing broad-band transmissive light through the leaf, a lens for focusing onto the imaging device the light emitted from the secondary light source, and one or more memory devices that store the fluorescence image and the transmitted light data received by the digital imaging device and store a library of plant fluorescence-intensity data indicative of both healthy plants and stressed or diseased plants, and plant light transmittance data indicative of certain plant conditions.

The invention discloses an early tobacco virus disease detection method based on an infrared thermal imaging technology, which comprises the steps that a tobacco infrared thermal image is subjected to image intensification from image smoothing, image sharpening and histogram equalization; a new tobacco leaf thermal image area and an old tobacco leaf thermal image area are extracted by a threshold segmentation method; if a temperature of a growth environment of tobacco is higher than 16 DEG C and less than or equal to 28 DEG C, a difference value between temperature values of various pixel points in the new tobacco leaf area and an average value of the temperature values is calculated; when an absolute value of the difference value is greater than 1 DEG C, a plant is a disease plant; if not, the plant is not the disease plant; when the environment temperature is higher than 28 DEG C and less than 34 DEG C, a difference value between average temperatures of the pixels in the new tobacco leaf area and the old tobacco leaf area is calculated; when the difference value is less than 0.8 DEG C, the plant is the disease plant; and if not, the plant is not the disease plant. The method is simple in step, short in consumed time and high in accuracy, can detect a disease earlier, can be applied to long-distance real-time monitoring of a field crop, and provides timely and reliable information of a crop growth condition, a disease condition and the like.

Disclosed is a method for cultivating pigment marigolds in the sand of a high and cold region. The method includes the steps that ground temperature rises and watering is sufficient 10 days before sowing; sowing is carried out on a fine day without wind, well-prepared soil is sieved by a sieve to cover a seedbed, a disinfectant is sprayed, a pesticide-clay mixture is scattered, and the seedbed is covered by straw; foliage top-dressing is conducted through DAP adding with water in the seedling stage, ventilation is achieved in a seedling growing greenhouse, films are uncovered, seedling exercising is conduced, seedling bacteria are sprayed so that damping-off can be prevented, soil insects can be trapped and killed by the way that phoxim is stirred and mixed with fried bran, and 200ppm of chlormequat chloride is sprayed for treating moderate seedling growth; compound fertilizer water is watered for facilitating growth, and the seedling age is 45-50 days for transplanting; field planting is carried out, the greenhouse is well built, soil preparation is completed, base fertilizer is fully applied, standard moisture in the soil is arranged according to the soil moisture width of 90cm, ponds are built on the water moisture surface in a twin-row mode according to the row spacing being 70cm and the line spacing being 60cm, film uncovering and seedling exercising are conducted 7 days before transplanting, and powder mancozeb is sprayed for disease prevention; field management is conducted, film rupturing and ridging are carried out, watering is achieved according to the dry and wet condition, diseased or infected plants are burned and buried, timber pile wires are pulled, the plants are fixed, and monopotassium phosphate is sprayed after defloration so that diseases can be prevented from endangering the plants. The method provides a mature technology for planting the marigolds in the sand of the high and cold region like Inner Mongolia.

A system (100), method and computer program product for determining plant diseases. The system includes an interface module (110) configured to receive an image (10) of a plant, the image (10) including a visual representation (11)of at least one plant element (1). A color normalization module (120) is configured to apply a color constancy method to the received image (10) to generate a color-normalized image. An extractor module (130) is configured to extract one or more image portions (11e) from the color-normalized image wherein the extracted image portions (11e) correspond to the at leastone plant element (1). A filtering module (140) configured: to identify one or more clusters (C1 to Cn) by one or more visual features within the extracted image portions (11e) wherein each cluster isassociated with a plant element portion showing characteristics of a plant disease; and to filter one or more candidate regions from the identified one or more clusters (C1 to Cn) according to a predefined threshold, by using a Bayes classifier that models visual feature statistics which are always present on a diseased plant image. A plant disease diagnosis module (150) configured to extract, byusing a statistical inference method, from each candidate region (C4, C5, C6, Cn) one or more visual features to determine for each candidate region one or more probabilities indicating a particulardisease; and to compute a confidence score (CS1) for the particular disease by evaluating all determined probabilities of the candidate regions (C4, C5, C6, Cn).

The invention provides a molecular detection primer of acidovorax avenae subsp. Citrulli and application thereof. The special primer comprises an upstream primer AIT1F: 5'-GCTGGATCACCTCCTTTCTG-3' and a downstream primer AIT2R: 5'-TGACGCAATCAAATTTTTGTCA-3'. Through PCG (Polymerase Chain Reation) amplification and agarose gel electrophoresis, a special amplification product with a fragment length of 462bp can be amplified in pure DNA of the acidovorax avenae subsp. Citrulli, a bacteria carrying disease plant tissue and a seed to rapidly detect the acidovorax avenae subsp. Citrulli. The special detection primer and the application thereof can be used for rapidly, sensitively and specially detecting the acidovorax avenae subsp. Citrulli in bacteria carrying watermelon seeds and plant seeds infected the acidovorax avenae subsp. Citrulli in production practice, can also be used for early diagnosis of field diseases and monitoring and identification of germs and provide a reliable technology and theoretical basis to control diseases caused by the acidovorax avenae subsp. Citrulli.

The invention provides loop-mediated isothermal amplification (LAMP) primers for detecting Phytophthora capsici and a kit comprising the primers. The primers comprise FIP, BIP, F3 and B3 (shown as Seq ID No.1-4). An LAMP primer technology is used for quickly detecting the Phytophthora capsici, and the Phytophthora capsici can be accurately detected in a complicated pathogenic bacteria environmentin diseased plant tissues and soil. The method has higher specificity and sensitivity than the conventional PCR method, propagules of the Phytophthora capsici in various forms, such as mycelia, oospores and zoospores can be detected, and the method has significance in aspects of early warning of Phytophthora capsici epidemics and monitoring pathogeny in epidemic areas; meanwhile, expensive instruments can be avoided, and the method is easily promoted and used in basic level.

The invention discloses bacillus siamensis X. The collection number of the bacillus siamensis X in the China Center for Type Culture Collection is CCTCC NO: M 2017008; a strain of the bacillus siamensis X is applied to the prevention and the treatment of the powdery mildew of the plant, compared with the existing chemical preventing and treating agent, the bacillus siamensis X is obvious in prevention and treatment effects, can be used for effectively controlling and relieving the state of an illness, can be used for making a disease plant recuperate health, further, is stable in the prevention and treatment effects, makes the recrudescence difficult, is safe, nontoxic, harmless and environment-friendly, and can be used for effectively avoiding pesticide-residue and environmental-pollution problems brought by the prevention and the treatment of a chemical pesticide; further, a preparation method of a product provided by the invention is simple, and is easy to operate; the bacillus siamensis X is suitable for industrialized production and market popularization and application.

The invention belongs to the technological field of agricultural production, and particularly relates to a method for preparing compound bactericide for preventing and controlling soybean root rot. The method comprises the following steps of: collecting field healthy soybean root soil, and specifically screening pseudomonas and bacillus for later use by using a King'B culture medium and a method for coating a beef extract peptone culture medium after 10 minutes of water bath at the temperature of 80 DEG C respectively; collecting roots of a field soybean root rot plant, and separating pathogenic fungi by using a martin culture medium; and re-screening bacterial strains for efficiently inhibiting the activity of the screened pathogenic fungi from the pseudomonas and the bacillus for the lateral use, fermenting the bacterial strains respectively, and then compounding the fermented bacterial strains according to a proportion to obtain the compound bactericide for preventing and controlling the soybean root rot. The screening method for preventing mixed bacteria by using soybean root growth can effectively improve the bioactivity and expand the control range; the compound bactericide is from the healthy soybean roots and most from soybean probiotics; and the colonization can be effectively performed on the soybean roots, so the utilization rate of the bactericide is enhanced.

The invention discloses a method for treating the citrus yellow shoot. Particularly, the method includes the following steps of 1, the soil sterilization process; 2, the soil improvement process; 3, the process of treating root systems of diseased plants; 4, the nutrition supplementing process conducted after the plants are cured. According to the method, the citrus yellow shoot in rutaceous plants (especially rutaceous fruit trees) can be effectively treated, and prevention and treatment effects on the aspects of soil bad bacterium treatment, plant root system phloem bacillus treatment, plant nutrition supplementing, maintenance after cure and the like are provided. The whole technology is complete, effective and safe, organic biological material products used in the treatment process are all safe and free of toxins, no other pesticide needs to be used, the production cost is reduced, harvested fruits can achieve the purpose of zero pesticide residue, the freshness and taste are kept, and the yield of the fruits is increased.

The invention discloses a planting method of carrot containing iodine. The method comprises the following steps: 1) high yielding and quality carrot seeds are selected, seta is removed from the seeds by kneading, then the seeds are dipped in mild water for accelerating germination and sowed when the seeds steep to germination; 2) ridges are started to be made, then 10-20 percent of germinated carrot feeds are evenly mixed with fine soil and sowed in lines, then the seeds are watered sufficiently and covered with soil evenly, ensuring that no seed exposes out of the soil; 3) thinning is made before final singling of seedlings to remove over close, weak, inferior and disease seedlings; 4) sufficient basic fertilizer is fertilized and solid iodine organic fertilizer is added to the surface of the soil in a lump before the carrot seeds are sowed in the field, or liquid iodine fertilizer is sprayed by stages before harvest, and cultivation and weeding as well as prevention and curing of plant diseases and insect pests are well done. The carrot containing iodine planted by the invention can not only replenish nutrition, which is good for physical health, but also can be an effective natural source of iodine for human body through food.

The invention provides a primer pair and a probe which are used for detecting phytophthora hibernalis and a detection method for phytophthora hibernalis, the nucleotide sequence of the primer pair is shown as SEQ ID No. 1 and 2, and the nucleotide sequence of the probe is shown as SEQ ID No. 3. The invention further provides a RT-PCR method for detecting phytophthora hibernalis. The method comprises: taking total DNA of a sample as a template, utilizing the above primer pair and probe to perform real-time fluorescence PCR amplification, acquiring data after each circulation is finished, and determining the result according to an amplification curve after the reaction is finished. The method is capable of rapidly accurately detecting phytophthora hibernalis from in a complicated pathogenic bacteria environment in diseased plant tissues and soil. A detection kit constructed according to the method is simple in operation, good in specificity and high in sensitivity, and has important meaning on aspects of early warning of phytophthora hibernalis, pathogeny monitoring in epidemic areas, import and export safety and the like.

The present invention discloses a method for preventing and treating kiwifruit canker. The method includes the following steps: A, microbial fertilizer application: a microbial fertilizer, i.e. a Dr. Ma microbial fertilizer, is applied to kiwifruits once in early September, once in middle-late October, once in late March and once in mid May; B, tree body and soil surface drip washing-type spray sterilization: the tree body and soil surface are subjected to drip washing-type spray sterilization once in early September, once in late October to late November and once in late February in the next year; and C, canker site scraping and sterilization: canker sites on the tree body are scraped, and the positions subjected to scraping are sterilized with alcohol and then applied with agricultural chemical liquid. The method can effectively prevent the occurrence of kiwifruit canker, has a high cure rate for diseased plants, and is simple and easy for fruit growers to use without harming the tree body.

The invention discloses a rice blast resistance gene Piym2 and application thereof, and provides the nucleotide sequence of the rice blast resistance gene Piym2 of paddy rice and the amino acid sequence of a protein coded by the rice blast resistance gene Piym2, and the gene is a member belonging to the CC-NBS-LRR resistance gene family. The invention also provides primers for amplifying the riceblast resistance gene Piym2 of paddy rice and the application of the gene in transforming paddy rice or other plants to breed disease-resistant varieties. When being transferred into diseased plants,the gene can help to produce new disease-resistant plants. In particular, hybridization and transformation methods can be used for adding a plurality of disease-resistant genes into plants, while theproblem of linkage drag in the genome, which appears in the conventional breeding technique, cannot occur, moreover, the breeding time can be shortened, and the variety-breeding efficiency can be increased.

The invention discloses a WRKY20 protein and application of an encoding gene thereof in regulating stress resistance of plants. The WRKY20 protein disclosed by the invention is a protein as shown in A1), A2), A3) or A4), wherein A1) is a protein, the amino acid sequence of which is a sequence 4, A2) is a protein, the amino acid sequence of which is a sequence 13, A3) is a protein, the amino acid sequence of which is a sequence 14, and A4) is a protein, the amino acid sequence of which is a sequence 21. Experiments verify that reduction of the WRKY20 protein content plays a role of improving resisting cotton bollworm, two-spotted spider mite and aphids and increase of the WRKY20 protein content plays a role of preventing bemisia tabaci, fungal diseases and geminivirus for plants, thereby providing a gene resource for culturing new species of insect-resistant and / or anti-disease plants. The protein has relatively good potential application value.

The invention provides a control method for a tobacco black shank disease. The prevention effect on the tobacco black shank disease is better, the incidence rate of the tobacco black shank disease is greatly reduced, the effect is good, and the yield and the quality of tobacco are improved. The method is carried out according to the following modes: (1) ecological soil management comprising (1.1) application of 200-300ml of Difulai per acre and (1.2) biological control namely watering of the root by using a high yield technology; (2) agricultural control; (3) physical control, wherein diseased plant residual bodies are removed and are destroyed in a gathered manner; (4) chemical control, wherein after transplanting and seedling returning, the root is watered for one time by using metalaxyl-mancozeb, and the root is watered by using propamocarb hydrochloride water or metalaxyl-propamocarb wettable powder in a tobacco-growing area where black shank germs show resistance to metalaxyl. According to the method, the tobacco black shank disease can be effectively prevented by the ecological soil management; meanwhile, comprehensive control is carried out by taking the measures of agricultural control, physical control and the like, so that the average incidence rate of the tobacco black shank disease is reduced, the yield is increased, the tobacco grade is remarkably improved, and good economical benefits are gained.