195 results about "Diseases plants" patented technology

A portable fluorescence and transmittance imaging spectroscopy system for use in diagnosing plant health. The system has a primary LED light source array with spectral wavelengths in the 400-600 nm range, a focus cone that collects the LED light source output and focuses it, a controller that controls the primary LED array to turn it on and off, or certain of the spectral wavelengths on and off such that the primary LED array controllably emits light of a desired wavelength in the range, the light irradiating the plant through the focus cone, a digital imaging device that both spatially and temporally captures a fluorescence image comprising chlorophyll fluorescence emitted by the plant due to the emitted light from the LED array, a leaf holder located proximate to the output of the focus cone to maintain a consistent position and distance between the digital imaging device, the LED light source and the leaf and providing for fixed position and non-destructive leaf imaging and testing, a secondary light source for providing broad-band transmissive light through the leaf, a lens for focusing onto the imaging device the light emitted from the secondary light source, and one or more memory devices that store the fluorescence image and the transmitted light data received by the digital imaging device and store a library of plant fluorescence-intensity data indicative of both healthy plants and stressed or diseased plants, and plant light transmittance data indicative of certain plant conditions.

The invention provides a CNN and transfer learning-based disease intelligent identification method and system, which can reduce interference of a picture background, can achieve high recognition accuracy under the condition of a limited sample number, and supports higher training sample multi-classification operation efficiency. The disease image identification method comprises the following steps: image preprocessing: normalizing the size of the image, quickly positioning a disease region by using Fast-RCNN, and eliminating background interference; image feature extraction: using a triplet similarity measurement model to extract image features, and then using SIFT features as compensation features to perform weighted fusion; and disease classification and recognition: learning a first image feature of a normal plant image by adopting a deep convolutional neural network, then learning a second image feature of the disease plant image by using transfer learning, and finally performing classification and recognition by combining the first image feature and the second image feature.

The invention discloses a method for obtaining sogatella furcifera carrying SRBSDV (southern rice black-streaked dwarf virus) and an application sogatella furcifera. The method for obtaining the sogatella furcifera carrying the SRBSDV, comprises the following steps of: feeding the sogatella furcifera by utilizing rice infecting the SRBSDV or cryopreserved rice diseased plant so that the sogatella furcifera obtains the SRBSDV, transferring the infected sogatella furcifera to a healthy rice to be fed till passing through a circulation period of virus, and detecting the sogatella furcifera by utilizing an RT-PCR (reverse transcription-polymerase chain reaction) technology and a serology method to obtain the sogatella furcifera carrying the SRBSDV, wherein the carrier rate of the sogatella furcifera can reach more than 50% through a manual feeding manner based on the experiment. Through utilizing the method for obtaining the sogatella furcifera carrying the SRBSDV, the obtained sogatella furcifera carrying the SRBSDV can be applied to screening of SRBSDV-resisting monoclonal antibody to carry out a rice inoculation experiment to identify the resistance of the rice, screen disease-resistant variety and provide a service of the breeding for disease resistance. The invention can be further applied to researching mutual relation between the SRBSDV and a vector insect sogatella furcifera and provides powerful theoretical evidence for prevention and treatment on the viruses.

The invention provides a prevention and control method of potato late blight, which is used for preventing and controlling potato late blight by comprehensive utilization of the traditional measures. The prevention and control method comprises the aspects of: potato seed treatment before sowing, management at seedling stage, cultivation measures, prediction and pre-warning, chemical control and the like. The prevention and control method is stable and permanent in prevention and control, reduces environmental pollution, is low in cost, and can predict and persistently control, thereby being amethod for realizing comprehensive prevention and control of potato late blight. From the growing conditions of the above-ground parts in field, the purpose of controlling the incidence and prevalence of late blight is achieved by adopting a late blight prediction and pre-warning system to instruct the chemical control on the field late blight. By adopting the prevention and control method, the disease plants are few, the yield is increased and is 25.7-45.8% higher than that of common planting, and the chemical spraying frequency is simultaneously reduced.

The invention relates to a single strand conformation polymorphism (SSCP) marker closely linked with a major wheat scab resistance quantitative trait locus (QTL). The SSCP marker is characterized in that: scab resistance candidate genes of a scab resistance QTL in a 3BS area of a wheat variety or strain resisting scab is PCR amplified by using a primer; after denaturalization, a PCR-amplified product has different single strand conformation; and the SSCP marker closely linked with the major wheat scab resistance QTL is established for detecting a genotype of the wheat variety or strain during breeding. The SSCP marker has the advantages of: overcoming the disadvantages that the wheat scab resistance screening can only be authenticated in a flowering period and is easily influenced by the environment in conventional breeding, predicting and screening wheat plants with the scab resistance by detecting a molecular marker at a seedling stage, eliminating disease plants, reducing waste of labor and materials and improving the breeding efficiency. Compared with an ABI DNA sequence analysis meter-based marker, the SSCP marker closely linked with the major wheat scab resistance QTL has the advantages of simple and convenient operation, low cost and same sensitivity.

The invention provides a method for laodelphax striatellus to obtain a rice black-streaked dwarf virus (RBSDV) from refrigerated diseased leaves. The principle of the invention is as follows: storing plant leaves carrying RBSDV by using a refrigerated method, obtaining RBSDV for laodelphax striatellus from refrigerated diseased leaves by a feeding method, and obtaining laodelphax striatellus with RBSDV applied in inoculating and identification after laodelphax striatellus passes a circulative period of virus. The varietalresistance identification method established by the invention can overcome the technical bottleneck of difficult long-term storage of diseased plants by adopting the method of obtaining the virus which feeds laodelphax striatellus with diseased plant better, broadens the varietalresistance identification time, and is hopeful for quickening the screening speed of disease-resistant resources and breeding process of disease-resistant varieties.

The invention relates to a loop-mediated isothermal amplification (LAMP) detection method for the specificity of radopholus similis and application thereof, and belongs to the technical field of biology. In the LAMP method for the specificity of the radopholus similis, LAMP detection primers for the specificity of the radopholus similis in a reaction system are RSF3, RSB3, RSFIP, RSBIP and RSLF respectively, and the radopholus similis is detected quickly and specifically by extracting the DNA of the radopholus similis or single-head radopholus similis in diseased plant tissues and performing LAMP isothermal amplification. Therefore, the detection method is high in specificity and sensitivity and low in cost, is easy and convenient to operate, and has the high application value in aspects of the on-site quick detection of the radopholus similis and the early diagnosis of radopholus similis diseases.

This invention relates to Bacillus subtilis and its application. Bacillus subtilis Jaas ed1 is stored in China General Microbiological Culture Collection Center on Dec. 12, 2005 with a number of CGMCC No.1564. Bacillus subtilis Jaas ed1 can enter crops with a low bacteria solution concentration, and colonize in crops. Bacillus subtilis Jaas ed1 has good effect on preventing plant diseases, especially soil-borne diseases. Besides, Bacillus subtilis Jaas ed1 can promote plant growth and produce various antibacterial metabolites, and has such advantages as low cost, easy operation, no pollution and no harm.

The invention discloses a potato breeder's seed seedling-raising substrate and a preparation method thereof, and belongs to the field of potato breeding and planting. The substrate comprises 20-40 parts of loam compound, 5 parts of potash fertilizer and 65-85 parts of specially-made organic fertilizer, wherein the loam compound comprises loam and a recycled substrate, the specially-made organic fertilizer is prepared by fermenting fallen leaves, manure, potato stems and leaves, coal ash powder and rice bran through microorganisms, and the fallen leaves include 60-80% of pine needles. The substrate mainly comprises the loam, the recycle substrate and the specially-made organism fertilizer. The specially-made organic fertilizer mainly comprises the fallen leaf pine needles, the manure and the potato stems and leaves free of diseased plants. The loam and the recycled substrate are exposed in sun and dried at high temperature, and the specially-made organic fertilizer is fermented at high temperature, so most bacteria, insect eggs and seeds can be killed, and convenient, clean and fertile substrate soil is provided for potato breeder's seed seedling-raising. The component raw materials of the substrate are wide in source, waste is reused due to the recycled substrate and the potato stems and leaves produced in seedling raising, and the substrate is quite suitable for being used and popularized in potato seedling raising bases in suburbs and countries.

A portable fluorescence and transmittance imaging spectroscopy system for use in diagnosing plant health. The system has a primary LED light source array with spectral wavelengths in the 400-600 nm range, a focus cone that collects the LED light source output and focuses it, a controller that controls the primary LED array to turn it on and off, or certain of the spectral wavelengths on and off such that the primary LED array controllably emits light of a desired wavelength in the range, the light irradiating the plant through the focus cone, a digital imaging device that both spatially and temporally captures a fluorescence image comprising chlorophyll fluorescence emitted by the plant due to the emitted light from the LED array, a leaf holder located proximate to the output of the focus cone to maintain a consistent position and distance between the digital imaging device, the LED light source and the leaf and providing for fixed position and non-destructive leaf imaging and testing, a secondary light source for providing broad-band transmissive light through the leaf, a lens for focusing onto the imaging device the light emitted from the secondary light source, and one or more memory devices that store the fluorescence image and the transmitted light data received by the digital imaging device and store a library of plant fluorescence-intensity data indicative of both healthy plants and stressed or diseased plants, and plant light transmittance data indicative of certain plant conditions.

A system (100), method and computer program product for determining plant diseases. The system includes an interface module (110) configured to receive an image (10) of a plant, the image (10) including a visual representation (11)of at least one plant element (1). A color normalization module (120) is configured to apply a color constancy method to the received image (10) to generate a color-normalized image. An extractor module (130) is configured to extract one or more image portions (11e) from the color-normalized image wherein the extracted image portions (11e) correspond to the at leastone plant element (1). A filtering module (140) configured: to identify one or more clusters (C1 to Cn) by one or more visual features within the extracted image portions (11e) wherein each cluster isassociated with a plant element portion showing characteristics of a plant disease; and to filter one or more candidate regions from the identified one or more clusters (C1 to Cn) according to a predefined threshold, by using a Bayes classifier that models visual feature statistics which are always present on a diseased plant image. A plant disease diagnosis module (150) configured to extract, byusing a statistical inference method, from each candidate region (C4, C5, C6, Cn) one or more visual features to determine for each candidate region one or more probabilities indicating a particulardisease; and to compute a confidence score (CS1) for the particular disease by evaluating all determined probabilities of the candidate regions (C4, C5, C6, Cn).

The invention provides a molecular detection primer of acidovorax avenae subsp. Citrulli and application thereof. The special primer comprises an upstream primer AIT1F: 5'-GCTGGATCACCTCCTTTCTG-3' and a downstream primer AIT2R: 5'-TGACGCAATCAAATTTTTGTCA-3'. Through PCG (Polymerase Chain Reation) amplification and agarose gel electrophoresis, a special amplification product with a fragment length of 462bp can be amplified in pure DNA of the acidovorax avenae subsp. Citrulli, a bacteria carrying disease plant tissue and a seed to rapidly detect the acidovorax avenae subsp. Citrulli. The special detection primer and the application thereof can be used for rapidly, sensitively and specially detecting the acidovorax avenae subsp. Citrulli in bacteria carrying watermelon seeds and plant seeds infected the acidovorax avenae subsp. Citrulli in production practice, can also be used for early diagnosis of field diseases and monitoring and identification of germs and provide a reliable technology and theoretical basis to control diseases caused by the acidovorax avenae subsp. Citrulli.

The invention provides loop-mediated isothermal amplification (LAMP) primers for detecting Phytophthora capsici and a kit comprising the primers. The primers comprise FIP, BIP, F3 and B3 (shown as Seq ID No.1-4). An LAMP primer technology is used for quickly detecting the Phytophthora capsici, and the Phytophthora capsici can be accurately detected in a complicated pathogenic bacteria environmentin diseased plant tissues and soil. The method has higher specificity and sensitivity than the conventional PCR method, propagules of the Phytophthora capsici in various forms, such as mycelia, oospores and zoospores can be detected, and the method has significance in aspects of early warning of Phytophthora capsici epidemics and monitoring pathogeny in epidemic areas; meanwhile, expensive instruments can be avoided, and the method is easily promoted and used in basic level.

The invention discloses bacillus siamensis X. The collection number of the bacillus siamensis X in the China Center for Type Culture Collection is CCTCC NO: M 2017008; a strain of the bacillus siamensis X is applied to the prevention and the treatment of the powdery mildew of the plant, compared with the existing chemical preventing and treating agent, the bacillus siamensis X is obvious in prevention and treatment effects, can be used for effectively controlling and relieving the state of an illness, can be used for making a disease plant recuperate health, further, is stable in the prevention and treatment effects, makes the recrudescence difficult, is safe, nontoxic, harmless and environment-friendly, and can be used for effectively avoiding pesticide-residue and environmental-pollution problems brought by the prevention and the treatment of a chemical pesticide; further, a preparation method of a product provided by the invention is simple, and is easy to operate; the bacillus siamensis X is suitable for industrialized production and market popularization and application.

The invention discloses a method for treating the citrus yellow shoot. Particularly, the method includes the following steps of 1, the soil sterilization process; 2, the soil improvement process; 3, the process of treating root systems of diseased plants; 4, the nutrition supplementing process conducted after the plants are cured. According to the method, the citrus yellow shoot in rutaceous plants (especially rutaceous fruit trees) can be effectively treated, and prevention and treatment effects on the aspects of soil bad bacterium treatment, plant root system phloem bacillus treatment, plant nutrition supplementing, maintenance after cure and the like are provided. The whole technology is complete, effective and safe, organic biological material products used in the treatment process are all safe and free of toxins, no other pesticide needs to be used, the production cost is reduced, harvested fruits can achieve the purpose of zero pesticide residue, the freshness and taste are kept, and the yield of the fruits is increased.

The invention provides a primer pair and a probe which are used for detecting phytophthora hibernalis and a detection method for phytophthora hibernalis, the nucleotide sequence of the primer pair is shown as SEQ ID No. 1 and 2, and the nucleotide sequence of the probe is shown as SEQ ID No. 3. The invention further provides a RT-PCR method for detecting phytophthora hibernalis. The method comprises: taking total DNA of a sample as a template, utilizing the above primer pair and probe to perform real-time fluorescence PCR amplification, acquiring data after each circulation is finished, and determining the result according to an amplification curve after the reaction is finished. The method is capable of rapidly accurately detecting phytophthora hibernalis from in a complicated pathogenic bacteria environment in diseased plant tissues and soil. A detection kit constructed according to the method is simple in operation, good in specificity and high in sensitivity, and has important meaning on aspects of early warning of phytophthora hibernalis, pathogeny monitoring in epidemic areas, import and export safety and the like.

The present invention discloses a method for preventing and treating kiwifruit canker. The method includes the following steps: A, microbial fertilizer application: a microbial fertilizer, i.e. a Dr. Ma microbial fertilizer, is applied to kiwifruits once in early September, once in middle-late October, once in late March and once in mid May; B, tree body and soil surface drip washing-type spray sterilization: the tree body and soil surface are subjected to drip washing-type spray sterilization once in early September, once in late October to late November and once in late February in the next year; and C, canker site scraping and sterilization: canker sites on the tree body are scraped, and the positions subjected to scraping are sterilized with alcohol and then applied with agricultural chemical liquid. The method can effectively prevent the occurrence of kiwifruit canker, has a high cure rate for diseased plants, and is simple and easy for fruit growers to use without harming the tree body.

The invention discloses a WRKY20 protein and application of an encoding gene thereof in regulating stress resistance of plants. The WRKY20 protein disclosed by the invention is a protein as shown in A1), A2), A3) or A4), wherein A1) is a protein, the amino acid sequence of which is a sequence 4, A2) is a protein, the amino acid sequence of which is a sequence 13, A3) is a protein, the amino acid sequence of which is a sequence 14, and A4) is a protein, the amino acid sequence of which is a sequence 21. Experiments verify that reduction of the WRKY20 protein content plays a role of improving resisting cotton bollworm, two-spotted spider mite and aphids and increase of the WRKY20 protein content plays a role of preventing bemisia tabaci, fungal diseases and geminivirus for plants, thereby providing a gene resource for culturing new species of insect-resistant and / or anti-disease plants. The protein has relatively good potential application value.

The invention provides a control method for a tobacco black shank disease. The prevention effect on the tobacco black shank disease is better, the incidence rate of the tobacco black shank disease is greatly reduced, the effect is good, and the yield and the quality of tobacco are improved. The method is carried out according to the following modes: (1) ecological soil management comprising (1.1) application of 200-300ml of Difulai per acre and (1.2) biological control namely watering of the root by using a high yield technology; (2) agricultural control; (3) physical control, wherein diseased plant residual bodies are removed and are destroyed in a gathered manner; (4) chemical control, wherein after transplanting and seedling returning, the root is watered for one time by using metalaxyl-mancozeb, and the root is watered by using propamocarb hydrochloride water or metalaxyl-propamocarb wettable powder in a tobacco-growing area where black shank germs show resistance to metalaxyl. According to the method, the tobacco black shank disease can be effectively prevented by the ecological soil management; meanwhile, comprehensive control is carried out by taking the measures of agricultural control, physical control and the like, so that the average incidence rate of the tobacco black shank disease is reduced, the yield is increased, the tobacco grade is remarkably improved, and good economical benefits are gained.

The invention provides a method for eliminating a wilt disease strain in a banana garden subjected to an incipient disease at high temperature. The method comprises the following steps that the banana wilt disease strain is isolated; at least 100 jin or above of boiled boiling water is sprayed to soil which surrounds the banana wilt disease strain and is 40-50 cm away from the stalk, and water spraying continuous to be conducted in a surrounding mode; glyphosate is injected into the sucker stem bases of plants beside the banana wilt disease strain; finally, paecilomyces varioti E7 and bacillus complex bacteria are blended into fermented organic fertilizer to obtain bacterial manure; after the banana wilt disease strain pending to be injected with the glyphosate and plants beside the banana wilt disease strain die, the bacterial manure is scattered in the place surrounding the stem within 40 cm, and withered leaves cover the manure. According to the technical scheme, for the banana garden subjected to the incipient disease, the diseased plants are treated in time, fusarium oxysporum diffusion is reduced, the diseased plants are effectively prevented from being damaged, and fusarium oxysporum diffusion and infection are effectively removed and prevented.

The invention discloses a method for controlling pests of greenhouse vegetables based on biological experiment of senior high schools. The method comprises the following steps of field cleaning: after the vegetables are harvested, timely collecting, burning or burying the disease plants which are left in the field; high-temperature greenhouse sealing: in high-temperature seasons of summer and spring, after fertilizer applying and soil furrowing in the greenhouse, tightly closing a plastic thin film, closing a greenhouse door and ventilating ports, rising the greenhouse temperature as much as possible, forming a high-temperature anaerobic environment, and killing multiple types of bacteria, nematodes and pests in the soil; when the pest attack occurs, using a biopesticide made by the inventor self to control. The method has the advantages that after proofing by field test, the control effect is good, the residue of pesticide is avoided, and the pollution to environment is avoided.

The invention relates to a citrus reiculata blanco planting method. The citrus reiculata blanco planting method specifically includes the steps of (1) seedling treatment: selecting disease-free citrus reiculata blanco seedlings with complete root systems, and soaking the root systems of the citrus reiculata blanco seedlings in a rooting liquid for 3-5 seconds before planting; (2) soil cultivation; (3) planting: (4) water and fertilization management; (5) branch and leaf trimming and flower and fruit protection: after fruit picking and before budding in a period from February to April in every year, trimming off intersecting branches, weak branches, diseased and wormy branches, dead branches and long branches germinated in summer, and removing diseased plants; (6) fruit picking and after-picking management. The citrus reiculata blanco planting method has the advantages that the method is simple and easy to operate, survival rate and fruit bearing rate of the plants can be increased, probability of occurrence of plant diseases and insect pests is lowered, the amounts of selenium and sugar contained in fruits are increased, fruit quality is improved, and high economic value of the fruits is obtained.

The present invention relates to the generation of transgenic plants resistant to infections caused by microorganisms restricted to the phloem, and it comprises the induction of the expression of a chimeric or fusion protein, which comprises a phloem protein of Citrus aurantifolia (CsPP16), a linker protein and a protein with antimicrobial activity; for example, with antibacterial activity or antibacterial hydrolytic enzyme. The portion of the fusion protein corresponding to the CsPP16 protein can exert its function of simplasmic movement, which occurs via the plasmodesmata of the plant to reach the phloem sieve tubes, while the antimicrobial portion can act to eliminate the microorganism, which settles in the phloem. The invention allows controlling the disease that causes the citrus yellowing or Huanglongbing (HLB), also applying to the treatment of other diseases caused by bacteria and other microorganisms that settle in the vascular tissue of higher plants. The invention includes the designed molecular vectors carrying and expressing a fusion protein and the transgenic plants obtained with a stable expression of said fusion protein and the transformation method of adult plants, healthy or diseased, to express the chimeric proteins and controlling, e.g., HLB disease. The transformation of diseased plants with the fusion protein is a method to produce healthy shoots free from the microorganism causing the disease; e.g., free from HLB bacteria.

The invention discloses a 'method for cultivating tilletia controversa kuhn disease plants in indoor environments', and belongs to the field of tilletia controversa kuhn control. The method includes steps of (1), preparing tilletia controversa kuhn germination winter spores; (2), preparing wheat seeds for tilletia controversa kuhn inoculation; (3), artificially inoculating wheat; (4), cultivating the disease plants in the indoor environments. The method for cultivating the tilletia controversa kuhn disease plants in the indoor environments has the advantages that the stable tilletia controversa kuhn morbidity can be acquired under indoor conditions, the method is beneficial to tilletia controversa kuhn biological research under the indoor isolation conditions, diffusion of germs during outdoor research can be prevented, and accordingly the method is favorable for controlling tilletia controversa kuhn diseases from various aspects.

An orange planting method provided by the invention comprises the following steps: (1) seedling treatment: selecting orange seedlings which have no diseases and have complete root systems as planting seedlings, and soaking the root systems into a rooting solution for 3-5 seconds before planting; (2) soil cultivation; (3) plantation; (4) water and fertilizer management; (5) branch and leaf trim, and flower and fruit protection: after fruit picking and before sprouting from February to April every year, trimming crossing branches, weak branches, insect-plague branches and dead branches, and trimming long branches which are sprouted in summer and removing diseased plants timely; and (6) fruit picking and management after the picking. The orange planting method provided by the invention has the advantages that the method is simple and practicable, can increase the survival rate and fruiting rate, reduce the occurrence probability of diseases and insect pests, increase the selenium content and sugar content of the fruits and increase fruit qualities, and has high economic values.

The invention discloses a pesticidal composition for treating fusarium wilt of cotton, which consists of the following raw materials in percentage by weight: 0.5 to 1.2 percent of 98 percent 3-indoleacetic acid, 0.2 to 0.6 percent of 50 percent naphthalene acetic acid, 1 to 3 percent of salicylic acid, 2 to 6 percent of plant activator protein, 35 to 59 percent of 40 percent acetic aldehyde and 35 to 59 percent of 55 percent white spirit. The pesticidal composition has the advantages of completely killing fusarium wilt bacteria within 30 minutes, stabilizing diseased plants without deterioration, beginning to recover the growth within five days, recovering within 8 to 12 days, controlling the recovery rate in a range from 85 to 93.5 percent and controlling the recurrence rate to be less than 11.5 percent.

The invention relates to a method for preventing and treating watermelon fusarium wilt, which belongs to the field of prevention and treatment of watermelon diseases. According to the technical scheme, the method comprises the following steps of: preparing a potassium permanganate, fulvic acid and fenaminosulf mixed aqueous solution as a liquid medicine; and performing root irrigation on diseased plants and nearby plants with the solution and spraying the solution to the leaves to all watermelons in the full field in the initial disease period, or spraying the solution to the leaves of the watermelons in the blossoming and fruit setting period in the full field for once every 5-7 days and for 2-3 times continuously. The method provided by the invention has the advantages of simpleness in preparation, low cost, low toxicity, no harm, convenience in use, good effect and the like.

The invention discloses a rapid and accurate detection method for Fusarium oxysporum based on a RPA (recombinase polymerase amplification)-lateral flow chromatography test strip technology. The methodcomprises the steps as follows: 1) extracting DNA of a to-be-detected sample; 2) performing RPA with DNA as a template; 3) detecting an amplification product by a lateral flow chromatography test strip; when two brown bands appear on the test strip, one is located in the quality control area and the other is located in the detection area, the result is positive and indicates that the sample contains the Fusarium oxysporum; when only a brown band appears in the control area of the test strip and no band is located in the detection area, the result is negative and indicates that the sample doesnot contain the Fusarium oxysporum. The primer probe group has good RPA effect and strong band specificity and has no cross reaction with other bacteria, positive reaction occurs in the detection area, detection sensitivity is increased, a new technical platform is provided for detection of the Fusarium oxysporum, pathogens are detected at the early stage of disease infection, and the Fusarium oxysporum in nursery grounds or diseased plants can be detected.

The invention discloses PCR detection primers for pantoea ananatis (pantoea ananatis) as pathogeny of bacterial wilt disease of mulberries and an application. The nucleotide sequence of the PCR detection primers for pantoea ananatis is as shown in SEQID NO:1-2. The invention provides specific PCR primers for detecting the pantoea ananatis as the pathogenic bacterium of the bacterial wilt disease of the mulberries, and through the primers, a detection method for the pantoea ananatis as the pathogenic bacterium of the bacterial wilt disease of the mulberries is established. The obtained primershave the advantages of being good in specificity and high in sensitivity, have excellent detection effects on DNA extracted from diseased plants and rhizosphere soil samples, which are infected with the pantoea ananatis as the pathogenic bacterium of the bacterial wilt disease of the mulberries, and have important significance on quick detection of the pantoea ananatis as the pathogenic bacteriumof the bacterial wilt disease of the mulberries in diseases of mulberries in a mulberry field.

The invention relates to a pesticide combination for preventing corn top rot, wherein the effective components of the pesticide combination for preventing the corn top rot comprises plant growth adjusting agents and active polysaccharides, the plant growth adjusting agents are one or two of compound nitrate phenolate and diethylaminoethyl hexanoate, and the active polysaccharides are one or two of chitosan oligosaccharide and portuguese polyene sugar. The pesticide combination for preventing the corn top rot comprises the components with part by weight: compound nitrate phenolate 0.05-90.0 parts and diethylaminoethyl hexanoate 0.05-90.0 parts. The pesticide combination for preventing the corn top rot induces basic resistance of corns by synergistically effects of the plant growth adjusting agents and the active polysaccharides, enhances ability of the corns for resisting environmental stress, promotes diseased plants to rapidly recover normal growth, and prevents and controls the corn top rot to happen and develop.