195 results about "Diseases plants" patented technology

A portable fluorescence and transmittance imaging spectroscopy system for use in diagnosing plant health. The system has a primary LED light source array with spectral wavelengths in the 400-600 nm range, a focus cone that collects the LED light source output and focuses it, a controller that controls the primary LED array to turn it on and off, or certain of the spectral wavelengths on and off such that the primary LED array controllably emits light of a desired wavelength in the range, the light irradiating the plant through the focus cone, a digital imaging device that both spatially and temporally captures a fluorescence image comprising chlorophyll fluorescence emitted by the plant due to the emitted light from the LED array, a leaf holder located proximate to the output of the focus cone to maintain a consistent position and distance between the digital imaging device, the LED light source and the leaf and providing for fixed position and non-destructive leaf imaging and testing, a secondary light source for providing broad-band transmissive light through the leaf, a lens for focusing onto the imaging device the light emitted from the secondary light source, and one or more memory devices that store the fluorescence image and the transmitted light data received by the digital imaging device and store a library of plant fluorescence-intensity data indicative of both healthy plants and stressed or diseased plants, and plant light transmittance data indicative of certain plant conditions.

The present invention relates to the generation of transgenic plants resistant to infections caused by microorganisms restricted to the phloem, and it comprises the induction of the expression of a chimeric or fusion protein, which comprises a phloem protein of Citrus aurantifolia (CsPP16), a linker protein and a protein with antimicrobial activity; for example, with antibacterial activity or antibacterial hydrolytic enzyme. The portion of the fusion protein corresponding to the CsPP16 protein can exert its function of simplasmic movement, which occurs via the plasmodesmata of the plant to reach the phloem sieve tubes, while the antimicrobial portion can act to eliminate the microorganism, which settles in the phloem. The invention allows controlling the disease that causes the citrus yellowing or Huanglongbing (HLB), also applying to the treatment of other diseases caused by bacteria and other microorganisms that settle in the vascular tissue of higher plants. The invention includes the designed molecular vectors carrying and expressing a fusion protein and the transgenic plants obtained with a stable expression of said fusion protein and the transformation method of adult plants, healthy or diseased, to express the chimeric proteins and controlling, e.g., HLB disease. The transformation of diseased plants with the fusion protein is a method to produce healthy shoots free from the microorganism causing the disease; e.g., free from HLB bacteria.

The invention discloses a rapid and accurate detection method for Fusarium oxysporum based on a RPA (recombinase polymerase amplification)-lateral flow chromatography test strip technology. The methodcomprises the steps as follows: 1) extracting DNA of a to-be-detected sample; 2) performing RPA with DNA as a template; 3) detecting an amplification product by a lateral flow chromatography test strip; when two brown bands appear on the test strip, one is located in the quality control area and the other is located in the detection area, the result is positive and indicates that the sample contains the Fusarium oxysporum; when only a brown band appears in the control area of the test strip and no band is located in the detection area, the result is negative and indicates that the sample doesnot contain the Fusarium oxysporum. The primer probe group has good RPA effect and strong band specificity and has no cross reaction with other bacteria, positive reaction occurs in the detection area, detection sensitivity is increased, a new technical platform is provided for detection of the Fusarium oxysporum, pathogens are detected at the early stage of disease infection, and the Fusarium oxysporum in nursery grounds or diseased plants can be detected.