Product
Patsnap Eureka
For R&D, Patsnap Eureka makes reading and utilizing patents & technical documents easy.
Patsnap Eureka AIR
Designed for self-driven R&D workflows. Generate viable solutions, solve complex R&D challenges, empower your innovation with AI.
Patsnap Eureka Materials
Designed for material experts only. Revolutionize your material R&D, from search, analyze, to developing new materials.
Feature
TechResearch
Generate reliable direction feasibility study reports for your R&D in just a few steps.
TechSeek
Discover and master advanced knowledge NOW. Basics, ideas, possibilities, all at once.
TechMind
As an expert in R&D Theories, TechMind can generates customized viable solutions instantly.
TechRisk
Analyze your overall solution with one click, know your potential R&D risks in advance.
TechMonitor
Get weekly tech updates, stay abreast of the latest tech innovations and key insights.
Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
36 results about "Tilletia" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Tilletia is a genus of smut fungi in the Tilletiaceae family. Species in this genus are plant pathogens that affect various grasses. Tilletia indica, which causes Karnal bunt of wheat, and Tilletia horrida, responsible for rice kernel smut, are examples of species that affect economically important crops.
Agricultural sterilizing composite containing thifluzamide
ActiveCN101828559AImprove securityMeet security requirementsBiocideFungicidesDiseaseAdditive ingredient
The invention discloses an agricultural sterilizing composite containing thifluzamide, containing a first active ingredient thifluzamide, and a second active ingredient selected from tebuconazole, difenoconazole, hexaconazole, propiconazole and cyproconazole, wherein the weight ratio of the first active ingredient to the second active ingredient is (12 : 1)-(1 : 12). The sterilizing composite is applied to controlling rice sheath blight disease, rice false smut, wheat sharp eyespot, wheat loose kernel smut and wheat Tilletia contraversa of cereal crops, and has reasonable ingredients, curing and protecting function, good sterilizing effect, less times of spraying insecticide and lower cost of spraying insecticide. In addition, the activity and the sterilizing effect of the sterilizing composite are not simple addition of the activity of the active ingredients, but remarkable synergism is achieved. The invention has good safety on plants, slows down the generation of resistance and meets the safety requirement on agrochemicals.
Owner:SHAANXI SUNGER ROAD BIO SCI
Agricultural compound fungicide
The invention belongs to the field of agricultural fungicide and particularly relates to an agricultural compound fungicide. The effective components of the fungicide are pyraclostrobin and fenpyrazamine which are compounded. The weight ratio of the two components is 1: 0.1-10, preferably, 1: 2-3. The prepared fungicide expands fungicide spectrum and has high activity on botrytis cinerea, rhizoctonia, puccinia, ustilago, tilletia, corticium, pyrenophora and other pathogenic fungi, and is remarkable in synergy which is not simple composition of activity of the components. The fungicide is strong in preventive treatment efficacy, long in effect lasting time, small in resistance risk and capable of reducing field usage of effective components, reducing production and using cost and reducing fungicide residue and environment pollution. The fungicide is favorable for overcoming and delaying production of bacteria drug resistance and is safe for crops due to effective components with different action mechanisms.
Owner:青岛润生农化有限公司
Method for identifying teliospores of Tilletia controversa Kuhn and Tilletia foetida
PendingCN105039491AMicrobiological testing/measurementMicroorganism based processesTriticeaeTriticum turgidum
The invention provides a method for identifying teliospores of Tilletia controversa Kuhn and Tilletia foetida and relates to the technical field of cell biology. The method includes the steps of 1, making slides, to be specific, smearing the teliospores of Tilletia controversa Kuhn and Tilletia foetida, respectively, after drying, dropwise adding oil camera oil to a microscopic portion, and covering the smears with cover slips; 2, performing observation, to be specific, placing the made slides under a laser confocal scanning microscope, selecting a proper scanning mode and scanning parameters to perform scan-observation and photographing, with the scanning mode being an xyz scanning mode and the scanning parameters including excitation wavelength and emission wavelength, the excitation wavelength of Tilletia controversa Kuhn being 488nm, the emission wavelength of Tilletia controversa Kuhn being 510 to 550nm, the excitation wavelength of Tilletia foetida being 488nm, and the emission wavelength of Tilletia foetida being 510 to 550nm. The method enables quick identification of the teliospores of Tilletia controversa Kuhn and Tilletia foetida.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Sterilizing composition
InactiveCN107691460ASynergistic effect is obviousReduce pollutionBiocideFungicidesDiseaseSeptoria musiva
The invention discloses a sterilizing composition. The effective components are Sedaxane and captan, the mass ratio of the both is 50:1-1:50. The composition has obvious synergism and is effective totreat diseases caused by rhizoctonia solani, puccinia horiana, pyrenophora graminea, ustilago esculenta, Tilletia, Erysiphe, pyrenophora teres, Septoria musiva, and Ramularia; the composition has special effect to seed-borne disease. The composition can be applied to treat seeds and spray stems and leaves, the effect duration lasts for 4-6 weeks; the composition can be applied to the disease prevention and treatment of cereal type crops; particularly, it is applied to the seed treatment for preventing wheat ustilago esculenta, wheat tilletia, wheat basal rot, sphacelotheca reiliana, corn stalkrot, corn gall smut and rice bakanae disease.
Owner:SHENZHEN NOPOSION AGROCHEM
Standard molecule for detecting tilletia walkeri castebury&carris and construction method thereof
InactiveCN102212611ASolve the problem of lackStable storageMicrobiological testing/measurementMicroorganism based processesAgricultural sciencePositive control
The invention discloses a standard molecule for detecting tilletia walkeri castebury&carris;. The standard molecule is a plasmid which can be duplicated, and the plasmid contains at least one of a sequence A (Seq ID N0.1) and a sequence B (Seq ID N0.2); the sequence A is 99.65% homologous with a 2.3kb nucleotide sequence of the mitochondrial DNA of the tilletia walkeri castebury&carris; and the sequence B is 100% homologous with the DNA sequence of a ribosomal internal transcribed spacer of the tilletia walkeri castebury&carris;. The invention also discloses a preparation method and detection method of the standard molecule. The standard molecule constructed in the invention can serve as a positive control for detecting the molecule of the tilletia walkeri castebury&carris; instead of the DNA of the tilletia walkeri castebury&carris; is suitable for qualitative and quantitative PCR (polymerase chain reaction) detection of the tilletia walkeri castebury&carris; and is suitable for the inspection and quarantine department of the port, the agricultural production department, the plant protection department and the like.
Owner:SHENZHEN AUDAQUE DATA TECH +1
Antigens and polyclonal antibody for discriminating Tilletia controversa Kuehn and Tilletia foetida
ActiveCN105348371AQuick judgmentAccurate judgmentSerum immunoglobulinsImmunoglobulins against animals/humansEuropean rabbitTilletia ayresii
The invention relates to antigens and a polyclonal antibody used for discriminating Tilletia controversa Kuehn and Tilletia foetida. A semiantigen used for preparing the polyclonal antibody for discriminating Tilletia controversa Kuehn and Tilletia foetida has an amino acid sequence as shown in SEQ ID No. 1. An immunogen used for preparing the polyclonal antibody for discriminating Tilletia controversa Kuehn is characterized in that the semiantigen mentioned above is reconstructed and coupled so as to obtain the immunogen, the immunogen is used to immunize domestic rabbits, domestic rabbits generating high-titer whole blood are screened and whole blood is acquired from a selected domestic rabbit and undergoes separating and purifying so as to obtain the polyclonal antibody. The antibody can specifically identify Tilletia controversa Kuehn without undergoing an antigen-antibody reaction with Tilletia foetida, so the polyclonal antibody can be used for discriminating Tilletia controversa Kuehn and Tilletia foetida difficult to distinguish in a field.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Detection primer, probe and detection method for tilletia indica mitra
InactiveCN101054599AShorten detection timeHigh sensitivityMicrobiological testing/measurementBiologyTilletia
Owner:上海出入境检验检疫局
Bactericidal composition containing tetramycin and thifluzamide
InactiveCN105076166ASynergistic effect is obviousDelay key drug resistanceBiocideFungicidesSuspending AgentsPlant disease
The invention discloses a bactericidal composition containing tetramycin and thifluzamide. Binary compounding is performed on the tetramycin and the thifluzamide of effective components of the bactericidal composition, and the remaining components are auxiliary components; the mass ratio of the tetramycin and the thifluzamide of the effective components of the bactericidal composition is (1-50):(50-1); the mass fraction ratio of the tetramycin to the thifluzamide of the effective components in the preparation is 1 percent to 80 percent, and the remaining components are the auxiliary components allowed to be used and accepted in pesticides. The bactericidal composition disclosed by the invention is missible oil, suspending agent, wettable powder, water dispersible granules, emulsion in water, microemulsion, granules, microcapsules and water aqua in dosage form; the bactericidal composition is mainly used for controlling powdery mildew of wheat, strawberries, grapes, apples and cucumbers, meanwhile, is capable of effectively controlling fungi, such as Rhizoctonia, Puccinia, Tolyposporium, Tilletia, Corticium and Pyrenophora, and especially has a special effect on diseases, such as banded sclerotial blight and damping off, caused by Basidiomycetes fungi.
Owner:HAILIR PESTICIDES & CHEM GRP
Fungicide composition containing metalaxyl and thifluzamide
InactiveCN104757005ASynergistic effect is obviousDelay key drug resistanceBiocideFungicidesWater dispersibleUstilago
The invention discloses a fungicide composition containing metalaxyl and thifluzamide. The fungicide composition comprises the effective components, including metalaxyl and thifluzamide which are compounded, and the balance of auxiliary components, wherein the mass ratio of the effective components metalaxyl and thifluzamide in the fungicide composition is (1-50) to (50-1), and the mass part ratio of the effective components metalaxyl and thifluzamide in the preparation is 1%-80%, and the balance is auxiliary components required to be used and accepted in pesticides. The fungicide composition has the dosage forms of missible oil, suspension, wettable powder, water dispersible granules, emulsion in water, microemulsion, granules, microcapsules and water aqua, is mainly used for preventing and controlling powdery mildew of wheat, strawberries, grapes, apples, and cucumbers, meanwhile can effectively prevent and control fungi such as rhizoctonia, puccinia, ustilago, tilletia, corticium, pyrenophora, and especially has special effects to banded sclerotial blight, damping off and the like due to basidiomycotina fungus.
Owner:HAILIR PESTICIDES & CHEM GRP
Plant disease control agent and plant disease control method
ActiveCN114025612AGood control effectGood storage stabilityBiocideDead animal preservationBiotechnologyBrassicaceae
This invention addresses the problem of providing an agent for controlling disease induced in a plant by a bacterium selected from the genus Rhizoctonia, the genus Fusarium, the genus Pythium, the genus Microdochium, the genus Tilletia, the genus Ustilago, the genus Streptomyces, the genus Erwinia, and the genus Aphanomyces, the plant being selected from cruciferous plants, beans, corn, wheat, potatoes, and sugar beets. In the present invention, this disease can be controlled by treating seeds, seedlings, or soil of these plants using a fungus belonging to the genus Talaromyces.
Owner:日本史迪士生物科学株式会社
Pesticide composition containing triticonazole
ActiveCN103283768AImprove stabilityAvoid harmBiocideDead animal preservationPlant diseaseHigh activity
The invention discloses a pesticide composition containing triticonazole. The composition is mainly characterized in that effective active components contain the triticonazole and an effective component B; the effective component B is selected from thiamethoxam, thiacloprid, imidacloprid and clothianidin, wherein the weight ratio of the triticonazole to the effective component B ranges from (1:90) to (30:1); the composition is prepared into a suspended seed coating. The composition disclosed by the invention can prevent and treat various underground insects and bacteria and has the obvious synergistic effect; an insecticidal spectrum is enlarged; the pesticide composition has high activity to the insects and the bacteria on wheat, corns, cotton, cucumbers and paddy rice; and diseases of the corns, the paddy rice and the cotton contain corn head smut, wheat smut, wheat powdery mildew, wheat tilletia and wheat net blotch. The pesticide composition has the slow release effect; the dosage of pesticides can be reduced; the pesticide composition is safe to people and livestock, has no phytotoxicity and is good in environmental compatibility.
Owner:陕西亿田丰作物科技有限公司
Bactericidal composition containing cyflufenamid and thifluzamide
InactiveCN103798256ASynergistic effect is obviousDelay key drug resistanceBiocideFungicidesSuspending AgentsUstilago
The invention relates to a bactericidal composition containing cyflufenamid and thifluzamide. The bactericidal composition comprises active ingredients including the cyflufenamid and the thifluzamide in a binary compound way, and the balance of auxiliary components, wherein the mass ratio of the active ingredient cyflufenamid to the active ingredient thifluzamide in the pesticide composition is (1-50): (50-1); the preparation comprises the active ingredients including the cyflufenamid and the thifluzamide with the mass percent of 1-80%, and the balance of acceptable auxiliary components which are allowed to be used in pesticide. The pesticide composition can be prepared into different preparations such as missible oil, suspending agent, wettable powder, water dispersible granules, emulsion in water, microemulsion, granules, microcapsules or aqueous solution, is mainly used for controlling the powdery mildew of wheat, strawberries, grapes, apples and cucumbers, is capable of effectively controlling fungi such as rhizoctonia, puccinia, ustilago, tilletia, corticium and pyrenophora, and especially has special effect for diseases such as banded sclerotial blight and damping off caused by basidiomycotina fungus.
Owner:HAILIR PESTICIDES & CHEM GRP
Primer, probe and method for detecting tilletia walkeri
InactiveCN101054600AShorten detection timeHigh sensitivityMicrobiological testing/measurementDNA/RNA fragmentationTeliosporeBiology
The present invention discloses specific primer and probe for detecting Tilletia walkeri and method for detecting Tilletia walkeri using the primer and probe. The inventive method can shorten greatly the detection time and promote the sensitivity. Single teliospore can be detected. Two kinds of pathogen teliospore can be simultaneously detected in the invention so detection efficiency is promoted.
Owner:上海出入境检验检疫局
Agricultural sterilizing composite containing thifluzamide
ActiveCN101828559BImprove securityMeet safety requirementsBiocideFungicidesPropiconazoleRice false smut
The invention discloses an agricultural sterilizing composite containing thifluzamide, containing a first active ingredient thifluzamide, and a second active ingredient selected from tebuconazole, difenoconazole, hexaconazole, propiconazole and cyproconazole, wherein the weight ratio of the first active ingredient to the second active ingredient is (12 : 1)-(1 : 12). The sterilizing composite is applied to controlling rice sheath blight disease, rice false smut, wheat sharp eyespot, wheat loose kernel smut and wheat Tilletia contraversa of cereal crops, and has reasonable ingredients, curing and protecting function, good sterilizing effect, less times of spraying insecticide and lower cost of spraying insecticide. In addition, the activity and the sterilizing effect of the sterilizing composite are not simple addition of the activity of the active ingredients, but remarkable synergism is achieved. The invention has good safety on plants, slows down the generation of resistance and meets the safety requirement on agrochemicals.
Owner:SHAANXI SUNGER ROAD BIO SCI
Preparation method of protoplast of Tilletia glabrata
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Antigens and polyclonal antibodies for differentiating Tilletia tritici and Tilletia tritici
ActiveCN105348371BQuick judgmentAccurate judgmentSerum immunoglobulinsImmunoglobulins against animals/humansTilletia triticiTilletia ayresii
The invention relates to antigens and a polyclonal antibody used for discriminating Tilletia controversa Kuehn and Tilletia foetida. A semiantigen used for preparing the polyclonal antibody for discriminating Tilletia controversa Kuehn and Tilletia foetida has an amino acid sequence as shown in SEQ ID No. 1. An immunogen used for preparing the polyclonal antibody for discriminating Tilletia controversa Kuehn is characterized in that the semiantigen mentioned above is reconstructed and coupled so as to obtain the immunogen, the immunogen is used to immunize domestic rabbits, domestic rabbits generating high-titer whole blood are screened and whole blood is acquired from a selected domestic rabbit and undergoes separating and purifying so as to obtain the polyclonal antibody. The antibody can specifically identify Tilletia controversa Kuehn without undergoing an antigen-antibody reaction with Tilletia foetida, so the polyclonal antibody can be used for discriminating Tilletia controversa Kuehn and Tilletia foetida difficult to distinguish in a field.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Standard molecule for wheat tilletia indica mitra detection and construction method thereof
InactiveCN102212518ASolve the problem of lackStable storageMicrobiological testing/measurementMicroorganism based processesBiotechnologyA-DNA
The invention discloses a standard molecule for wheat tilletia indica mitra detection. The standard molecule is plasmid capable of being replicated, the plasmid contains at least one of sequences A and B, the homology of the A (Seq ID No. 1) and a nucleotide sequence (AF218059) of the last fragment 2.3kb of wheat tilletia indica mitra mitochondria DNA is 99.56 percent, and the homology of the B (Seq ID No. 2) and a DNA sequence (AF399888) of a transcribed spacer in wheat tilletia indica mitra ribosome is 99.85 percent. The invention also discloses a preparation and detection method of the standard molecule. The constructed standard molecule can be used for substituting wheat tilletia indica mitra DNA and used as positive control of wheat tilletia indica mitra molecular detection, is suitable for qualitative polymerase chain reaction (PCR) detection and quantitative PCR detection of wheat tilletia indica mitra, and is suitable for port inspection and quarantine, agricultural production, plant protection and the like.
Owner:SHENZHEN AUDAQUE DATA TECH +1
A kind of pesticide composition containing fenconazole
The invention discloses a pesticide composition containing triticonazole. The composition is mainly characterized in that effective active components contain the triticonazole and an effective component B; the effective component B is selected from thiamethoxam, thiacloprid, imidacloprid and clothianidin, wherein the weight ratio of the triticonazole to the effective component B ranges from (1:90) to (30:1); the composition is prepared into a suspended seed coating. The composition disclosed by the invention can prevent and treat various underground insects and bacteria and has the obvious synergistic effect; an insecticidal spectrum is enlarged; the pesticide composition has high activity to the insects and the bacteria on wheat, corns, cotton, cucumbers and paddy rice; and diseases of the corns, the paddy rice and the cotton contain corn head smut, wheat smut, wheat powdery mildew, wheat tilletia and wheat net blotch. The pesticide composition has the slow release effect; the dosage of pesticides can be reduced; the pesticide composition is safe to people and livestock, has no phytotoxicity and is good in environmental compatibility.
Owner:陕西亿田丰作物科技有限公司
Standard molecule for detecting tilletia walkeri castebury&carris and construction method thereof
InactiveCN102212611BSolve the problem of lackStable storageMicrobiological testing/measurementMicroorganism based processesBiotechnologyQuarantine
The invention discloses a standard molecule for detecting tilletia walkeri castebury&carris;. The standard molecule is a plasmid which can be duplicated, and the plasmid contains at least one of a sequence A (Seq ID N0.1) and a sequence B (Seq ID N0.2); the sequence A is 99.65% homologous with a 2.3kb nucleotide sequence of the mitochondrial DNA of the tilletia walkeri castebury&carris; and the sequence B is 100% homologous with the DNA sequence of a ribosomal internal transcribed spacer of the tilletia walkeri castebury&carris;. The invention also discloses a preparation method and detection method of the standard molecule. The standard molecule constructed in the invention can serve as a positive control for detecting the molecule of the tilletia walkeri castebury&carris; instead of the DNA of the tilletia walkeri castebury&carris; is suitable for qualitative and quantitative PCR (polymerase chain reaction) detection of the tilletia walkeri castebury&carris; and is suitable for the inspection and quarantine department of the port, the agricultural production department, the plant protection department and the like.
Owner:深圳出入境检验检疫局动植物检验检疫技术中心 +1
Method of artificially inoculating Tilletia tritici to infect wheat
ActiveCN105409618BHigh incidence of diseaseIncrease success rateMicrobiological testing/measurementHorticulture methodsSporelingTilletia tritici
The invention provides a method for artificial inoculation of Tilletia foetida infected wheat and belongs to the field of agricultural microbiology. The method includes: (1), preparing a Tilletia foetida hypha suspension for infection, namely crushing mycocecidium of Tilletia foetida, preparing a spore suspension, coating the spore suspension on a 2%water agar medium, placing the water agar medium in an incubator at 16 DEG C for incubation until teliospores germinate and hyphae grow out, and using sterilized distilled water to flush down the hyphae for standby use; (2), preparing for inoculation of wheat seedlings, namely soaking wheat seeds until sprouting, subjecting the wheat seeds to vernalization at 5 DEG C for one month, planting the vernalized seeds in sterilized soil, and taking the wheat seedlings when they grow to a jointing-booting stage for standby use; (3), inoculating, namely injecting the Tilletia foetida hypha suspension obtained in the step (1) into a gap of pollen chambers of wheat plants. By adopting the method, success rate of obtaining diseased wheat plants is higher. The method is simple and easy to implement, convenient to implement and operate and conducive to popularization and application.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
A method for distinguishing teliospores of Tilletia dwarfis and teliospores of Tilletia glabris
The present invention relates to the field of cell biology technology, and specifically relates to a method for identifying teliospores of wheat dwarf smut and teliospores of wheat light smut. It includes the following steps: stain the samples to be tested that are suspected to be teliospores of P. triticifolia or teliospores of P. triticifolia in WGA‑AF488 dye, then rinse with PBS, and then scan under a confocal laser microscope. Observe; if the ridges and cell walls are both dyed green, the sample to be tested is teliospores of P. tritici; if only the cell wall is dyed green, the sample to be tested is teliospores of P. tritici. The staining results of this method are clear and can quickly and accurately distinguish TCK teliospores and TFL teliospores.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Molecular detection method for two Tilletia foetida similar species on bentgrass
InactiveCN101328494AQuick checkEasy to detectMicrobiological testing/measurementDNA/RNA fragmentationAgrostisHypha
The invention relates to a method for detecting Tilletia sphaerococca (Wallr)Waldh and T.caries (TCT) in Gramineae grass seed Agrostis spp L. by adopting the PCR primer technique, belonging to the Gramineae grass seed Tilletia detection field. The method designs five sets of PCR primers of a common outer primer, a common inner primer, an outer primer and a special primer of T.sphaerococca, and a special primer of the TCT, wherein, the common inner primer is used for the shell type amplification of the Tilletia teliospore so that the quality monitoring of the amplification process is realized to avoid false negative; the special primer of the T.sphaerococca and the special primer of the T.caries are used for the hypha genome DNA double special PCR amplification; each outer primer and each special primer are used for detecting two teliospores of the double shell type PCR. The method has high detection speed and is reliable; and the whole detection process is finished within one working day.
Owner:ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Sterilizing composition containing sedaxane and fluopicolide
InactiveCN108077267ASynergistic effect is obviousDelay key drug resistanceBiocideFungicidesDiseaseSuspending Agents
The invention relates to a sterilizing composition containing sedaxane and fluopicolide. The active component of the sterilizing composition is formed by compounding the sedaxane and the fluopicolide,and the rest is auxiliary components. The mass ratio of the active components, namely the sedaxane to the fluopicolide, of the pesticide composition is (1-50):(50-1), the mass part ratio of the active components, namely the sedaxane to the fluopicolide, in the preparation is 1 percent to 80 percent, and the rest is auxiliary components which are allowed to use and accept in the pesticide. The dosage forms of the pesticide composition are missible oil, a suspending agent, and the pesticide composition is mainly used for preventing and treating powdery mildew on triticeae, strawberries, grapes,apples and cucumbers; and moreover, the pesticide composition can effectively prevent and control fungi such as rhizoctonia, puccinia person, ustilago, tilletia, corticium and pyrenophora, in particular has a special effect on diseases, such as rhizoctonia solani and damping off, caused by basidiomycetes.
Owner:SHANGDONG HAILIER CHEM
Bactericidal composition
The invention discloses a bactericidal composition, which is prepared from triticonazole and fludioxonil as effective components, and the mass ratio of triticonazole to fludioxonil is (50:1)-(1:50). The composition disclosed by the invention has obvious synergistic effects, is effective to diseases caused by fusarium, ustilago, tilletia, pyrenophora teres, septoria, rhizoctonia solani and the like, is mainly used for preventing and treating diseases of cereal crops, leguminous crops and fruit trees, has special effects on seed-borne diseases, has a lasting period of 4-6 weeks, and can be usedfor preventing and treating diseases of cereal crops and especially preventing and treating ustilago tritici, stinking smut, wheat stem rot, maize head smut, maize stem rot, maize gall smut and rice bakanae disease in seed treatment.
Owner:SHENZHEN NOPOSION AGROCHEM
Method for detecting TilletiafuscaEII&EV and T.bromi(Brockm.)Brockm by using double polymerase chain reaction (PCR) primers
InactiveCN102229998AQuick checkEasy to detectMicrobiological testing/measurementDuplex pcrGenomic DNA
The invention relates to a method for detecting TilletiafuscaEII&EV and T.bromi(Brockm.)Brockm by using a double PCR amplification technique and belongs to the field of detection of tilletia of grass family. In the invention, six primers, namely a universal outer primer, a universal inner primer, an outer primer of T.fusca, a specific primer of T.fusca, an outer primer of T.bromi and a specific primer of T.bromi, are designed. The universal outer primer is used for nested amplification of a hypha genomic DNA or teliospore, the universal inner primer is used for the nested amplification of teliospore, and the universal outer primer and the universal outer primer are used for quality monitoring in an amplification process and detecting the quality of extracted hypha genomic DNA and selection and breakage of teliospore and can avoid false negative in a detection process. The outer primers and specific primers of T.fusca and T.bromi can be used for the double specific PCR amplification of the hypha genomic DNA of the two kinds of bacteria, and the respective outer primers and specific primers are used in double nested PCR specific amplification of DNA of teliospore.
Owner:ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Agrobacterium-mediated tilletia foetida transformation method
The invention relates to tilletia foetida genetic transformation, and more specifically relates to an agrobacterium-mediated tilletia foetida transformation method. The method comprises the followingsteps: culturing TFL teliospore, when a germination rate is greater than or equal to 60%, collecting mycelia, and preparing 1*106 / ml mycelia liquid; inoculating agrobacterium in a LB medium, performing oscillation culture for 2-3 d at the temperature of 28 DEG C, diluting the material to OD600 being 0.5 by using an IM medium, continuously culturing for 7-8 h, and using the IM medium for diluting the material to OD600 being 0.45-0.5; spreading a layer of aseptic glassine paper at the CM medium, performing isopyknic mixing of agrobacterium bacteria liquid and mycelia liquid and adding AS to 200[mu]mol / l, coating the material at the glassine paper, culturing the material in dark for 24 h at the temperature of 22 DEG C; transferring the glassine paper to a novel CM medium, applying one surface coated with the bacteria liquid to the medium, and culturing the material at the temperature of 16 DEG C until bacterium colony is grown. The method successfully transfers the exogenous gene to thetilletia foetida genome and realizes stable heredity.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Method for infecting wheat by inoculating tilletia foetida to roots
ActiveCN105325196AIncrease success rateSimple methodMicrobiological testing/measurementMicroorganism based processesTriticeaeHypha
The invention relates to a method for infecting wheat by inoculating tilletia foetida to roots, which is characterized by comprising the following steps: (1) culturing hypha of tilletia foetida: coating a 2% water agar culture medium with tilletia foetida teleutospore suspension with concentration of 1*10<5> / ml, culturing at 16 DEG C, and observing and confirming until promycelium grows, wherein 0.1-0.5ml of suspension is added to the culture dish of every 9cm diameter, and the promycelium is used for inoculation in the next step when the size of the promycelium is 1 / 2 the diameter of the teleutospore; (2) inoculating: placing sprouted wheat seeds to the culture medium in which the hypha grows, placing the culture dish to a 5 DEG C incubator, and simultaneously carrying out vernalization and infection for 30-45d; and (3) after vernalization is finished, planting wheat seedlings on sterile soil, and culturing in an artificial climate incubator with day temperature 23 DEG C and night temperature 21 DEG C, wherein the sprouted wheat seeds are sprouted wheat with coleoptile not more than 1mm. By adopting the method, wheat plants with roots infected by tilletia foetida and other parts presenting normal are obtained.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
The method for obtaining the diseased plants of T. dwarfis by indoor cultivation
ActiveCN104221648BEffectively assess the risks of productionStable incidenceHorticulture methodsSpore processesDiseaseDiseased plant
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Agrobacterium tumefaciens-mediated transformation method for Tilletia contraversa Kuhu
The invention relates to genetic transformation of Tilletia contraversa Kuhu, and specially relates to an agrobacterium tumefaciens-mediated transformation method for the Tilletia contraversa Kuhu. The method includes the following steps: culturing TCK teliospores, collecting mycelia when the germination rate is more than or equal to 60%, and preparing a mycelium liquid with 1*10<6> / ml; inoculating the agrobacterium tumefaciens in an LB culture medium, performing shake cultivation at 28 DEG C for 2-3 d, diluting with an IM culture medium until OD<600> is 0.15-0.35, continuing to culture for 7-8 h, and diluting with the IM medium to the OD<600> is 0.53-0.59; laying a layer of sterile cellophane on a CM medium, isovolumetrically mixing an agrobacterium tumefaciens liquid with the mycelium liquid, coating the cellophane with the mixture, and performing darkness culture at 20-22 DEG C for 48 h; and then transferring the cellophane to a new CM medium to make one side coated with a bacterialsolution attach to the culture medium, and performing the darkness culture at 5 DEG C until a bacterium colony grows. According to the method, foreign genes can be successfully transferred into a genome of the Tilletia contraversa Kuhu and can be stably inherited.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
A kit and method for detecting Tilletia dwarf
ActiveCN104593332BTissue cultureImmunoglobulins against fungi/algae/lichensBiotechnologyTilletia tritici
The invention "a kit and method for detecting Tilletia dwarfis" belongs to the detection technology of plant pathogens. Including a test strip and a microporous plate, the test strip includes a bottom plate, a sample absorbent pad, a reaction film and a water absorbent pad; the sample absorbent pad, reaction film, and water absorbent pad are sequentially pasted and installed on the bottom plate; its features In that: there are freeze-dried micropore reagents in the microwells of the micropore plate, and the micropore reagents refer to gold-labeled antibodies obtained by labeling the monoclonal antibody described in claim 2 with colloidal gold; the reaction membrane is divided into A detection area and a quality control area, the detection area is coated with Tilletia tritici polyclonal antibody, and the quality control area is coated with anti-antibody. The kit of the invention has good specificity and very low detection limit, and is suitable for the prevention and control or quarantine of T. tritici.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com