40results about How to "Solve the problem of lack" patented technology

The invention discloses total mixed pellet feed for meat sheep and a manufacturing method and application of the feed. The feed comprises: 20.00-40.00% of straw, 0.00-30.00% of caragana korshinskii, 0.00-30.00% of sunflower shells, 0.00-30.00% of leymus chinensis, 10.00-30.00% of corn, 5.00-10.00% of molasses, 5.00-8.00% of soybean meal, 0.00-10.00% of cottonseed meal, 0.00-5.00% of sesame meal, 0.00-15.00% of maize germ meal, 0.00-5.00% of rapeseed meal, 0.00-8.00% of corn bran with slurry, 0.00-3.00% of wheat bran, 0.00-5.00% of rice bran, 0.00-20.00% of corn distiller's dried grains with soluble, 0.00-3.00% of bentonite, 0.40-0.80% of salt, 0.10-0.50% of calcium hydrogen phosphate, 0.50-1.50% of stone flour, 0.00-0.80% of slow-release protein, 0.50-1.50% of sodium bicarbonate, 0.04-0.08% of a mildew inhibitor, 0.40-0.50% of ammonium chloride, 1.00-3.00% of Chinese herbal medicine premix and 0.80-1.50% of meet goat fattening premix. The feed can replace common refined feeding and roughage, the breeding process is simplified, the breeding cost is saved, and the total mixed pellet feed can prevent the occurrence of rumen acidosis and urinary calculus of meet sheep.

The invention discloses an occluded pedestrian re-identification method based on massed learning and deep network learning. According to the method, multiple types of occluded training samples are generated from original non-occluded training samples through an occlusion simulator, the generated occluded training samples and the original training samples form a united training set used for model training, meanwhile, occluded and non-occluded classification losses are added into pedestrian classification losses, and a multi-task loss function is used to replace a single-task loss function in the past. In this way, pedestrian re-identification under occlusion is effectively coped with, and priori information of occlusion and non-occlusion is considered to perform feature extraction during deep network feature learning. Experiments indicate that through the method, the performance of an existing deep network in occluded pedestrian re-identification can be substantially improved, and the method has high application value.

The invention relates to a method for promoting growth of plants on manganese tailing residues. Used indigenous bacteria come from the tailing residues on which plants do not grow. Used plants are Phytolacca americana. An inoculated water-retaining gel bacteria bed and the tailing residues are evenly mixed according to a mass ratio being 1: (4-8) to cultivate Phytolacca americana seedlings. The plants can be promoted to grow and breed on the manganese tailing residues to form vegetation. The water-retaining gel bacteria bed contains 5 parts of white mica, 5 parts of montmorillonite, 7 parts of agar and 83 parts of indigenous bacteria culture solution. The indigenous bacteria culture solution contains soluble starch with concentration being 5g / L, beef tallow cream with concentration being 1g / L, peptone with concentration being 3g / L, dipotassium hydrogen phosphate with concentration being 0.2g / L, sodium chloride with concentration being 5g / L, cane sugar with concentration being 3g / L, magnesium sulfate with concentration being 0.2g / L, ferrous sulfate with concentration being 0.2g / L, potassium nitrate with concentration being 0.5g / L, urea with concentration being 10g / L and calcium superphosphate with concentration being 5g / L. The method has the advantages that the operation is simple, the cost is low, the efficiency of resisting and controlling the migration and the diffusion of manganese and other harmful metal in manganese tailing residues, the environment can be beautified and the like.

The invention provides a lightning current online monitoring system of an overhead transmission line based on a differential ring, relating to a structure of the lightning current online monitoring system of the overhead transmission line. The lightning current online monitoring system mainly comprises a current sensor, a lightning stroke signal collecting unit, a data receiving and GPRS (General Packet Radio Service) transmission unit, a power supply unit and a user unit. With the adoption of the lightning current online monitoring system provided by the invention, lightning current of the overhead transmission line is monitored online in a non-contact manner. The lightning current online monitoring system has the advantages of being safe to use, reliable in work, intelligent in operation, high in monitoring accuracy, high in sensitivity and high in precision, can help staff to rapidly troubleshoot a lightning fault of the overhead transmission line to reduce loss, and has the characteristics of simple circuit structure, low cost and convenience in popularization and application and the like. The lightning current online monitoring system provided by the invention can be widely applied to the lightning current online monitoring system of the overhead transmission line, and is particularly suitable for the lightning current online monitoring system of the 110-500 kV high-voltage overhead transmission line.

The invention discloses polynucleotide, a method and a kit for detecting listeria monocytogenes. Specifically, the invention discloses polynucleotide, which can be used as a standard molecule in realtime fluorescent PCR detection of listeria monocytogenes, and DNA constructs (LY01 and LY02). The provided plasmid standard molecule can solve the problem that realtime fluorescence PCR detection of listeria monocytogenes is lack of standard substances, and moreover, can guarantee the comparability of the detection results of realtime fluorescence PCR detection. A reliable quality control method is provided for the realtime fluorescence PCR detection of listeria monocytogenes.

The invention relates to a high-powered recycling process of coal-chemical industry clean wastewater and a special device for the high-powered recycling process. The coal-chemical industry clean wastewater is subjected to multistage clarification, ultrafiltration and osmosis treatment, the water recovery rate of the clean wastewater ultimately reaches above 95%, the purpose of high-powered recycling of the coal-chemical industry clean wastewater is achieved, the water resource is saved and the difficult problem of the deficiency of the fresh water resource is solved.

The invention discloses a polynucleotide, a method and a kit for detecting Bacillus anthraci, and particularly discloses the polynucleotide which can be used as a standard molecule for real-time fluorescent PCR (polymerase chain reaction) detection of the Bacillus anthraci as well as DNA constructs PA and capA. By the aid of the plasmid standard molecule, the problem of lack of standard substances for real-time fluorescent PCR detection of the Bacillus anthraci is solved, the comparability of the detection result of a real-time fluorescent PCR method of the Bacillus anthraci is guaranteed, and a reliable quality control method is provided for detection with the real-time fluorescent PCR method of the Bacillus anthraci.

The invention provides a selenium-rich high-dietary-fiber recombinant rice and a processing method thereof. The processing method comprises the following steps: uniformly mixing the following raw material powder in parts by weight, adding water which accounts for 20-35% of the weight of the mixed raw material powder for hardening and tempering, putting the mixed raw material powder into a double-screw extrusion granulator for low-temperature extrusion to form rice grains, and carrying out air draft drying at 35-65 DEG C for 1-2 hours to obtain the selenium-rich high-dietary-fiber recombinant rice with the dietary fiber content of 6-15% and the selenium content range of 18-30mu g / 100g. The mixed raw material powder is prepared from the following raw material powder in percentage by weight: rice flour, whole grain powder, medicinal and edible food powder, bean powder, potato powder, dietary fiber powder and a selenium-enriched oyster mushroom enzyme hydrolysis extract. The selenium-rich high-dietary-fiber reprocessed rice can well solve the problem of a supplement source of selenium element for people in selenium-deficient areas, and can be used as a supplement source of dietary fibers for people with insufficient dietary fiber intake, so as to achieve the purpose of supplementing the dietary fibers.

The invention discloses polynucleotide, a method and a kit for detection of a bacterial drug-resistant gene, namely, a New Delhi metallo-blactamase-1 (NDM-1) gene. Specifically, the invention discloses polynucleotide and a DNA construction material pXL08 capable of being used as real-time fluorescent PCR detection standard molecules for the bacterial drug-resistant gene NDM-1. According to the invention, due to plasmid standard molecules, the difficult problem of lack of standard substances in the real-time fluorescent PCR detection of the bacterial drug-resistant gene NDM-1 is solved, the comparability of a real-time fluorescent PCR detection result of the bacterial drug-resistant gene NDM-1 is ensured, and a reliable quality control method is provided for the real-time fluorescent PCR detection of the bacterial drug-resistant gene NDM-1.

The invention discloses a polynucleotide, method and kit for detecting transgenic crops. Specifically, the present invention discloses the sequences of the polynucleotide construct pLW10 and its matching primer probes that can be used as standard molecules for real-time fluorescent PCR detection of transgenic crops. The plasmid standard molecule of the present invention solves the problem of lack of standard substances in the real-time fluorescent PCR detection of transgenic crops, ensures the comparability of the detection results of the real-time fluorescent PCR method of the transgenic crops, and provides a reliable quality control method for the real-time fluorescent PCR detection of the transgenic crops.

The invention discloses a plasmid standard molecule of enterobacter sakazakii, a preparation method and a quantification method of the plasmid standard molecule and applications thereof. An obtained standard substance is a plasmid standard molecule pXL04, wherein the sequence of the plasmid standard molecule pXL04 is represented by SEQ ID NO: 2 in a sequence table. According to the plasmid standard molecule of enterobacter sakazakii, the plasmid standard molecule pXL04 is quantified by using an ultraviolet spectrophotometric method and a high-resolution inductive coupling plasma emission mass spectrum (HR-ICP-MS) so as to ensure the good traceability. According to the plasmid standard molecule, the difficulty that the standard substances are lacked in real-time fluorescence quantification PCR detection of the enterobacter sakazakii is overcome; the comparability of a detection result of a real-time fluorescence quantification PCR detection method of the enterobacter sakazakii is ensured and a reliable quality control method is provided for the detection of the real-time fluorescence quantification PCR detection method of the enterobacter sakazakii.

The invention discloses polynucleotide, a method and a kit for detecting salmonella bacteria. Specifically, the invention discloses polynucleotide and DNA constructs pXL10 and pXL11, which can be used as standard molecules for real-time fluorescent PCR (polymerase chain reaction) detection of the salmonella bacteria. The plasmid standard molecules disclosed by the invention solve the difficulty that the real-time fluorescent PCR detection of the salmonella bacteria lacks standard substances, thereby ensuring the comparability of a result of a real-time fluorescent PCR method detection of the salmonella bacteria, and providing a reliable quality control method for the real-time fluorescent PCR method detection of the salmonella bacteria.

The invention discloses a plasmid standard molecular adaptable to sakazakii real-time fluorescent quantitation PCR (polymerase chain reaction) detection, a preparation method thereof, a quantitation method thereof and application. The plasmid standard molecular comprises a 16S rDNA gene sequence of the sakazakii. The 16S rDNA gene sequence is shown as SEQ ID NO: 1 in sequence lists. The plasmid standard molecular is quantitated by the ultraviolet light spectrophotometry method and a high resolution inductively coupled plasma emission mass spectrometry (HR-ICP-MS), and good traceability is guaranteed. By the aid of the plasmid standard molecular, the problem of the lack of standard material during the sakazakii real-time fluorescent quantitation PCR (polymerase chain reaction) detection, comparability of results of the sakazakii real-time fluorescent quantitation PCR detection, and a reliable quality control method is provided for the sakazakii real-time fluorescent quantitation PCR detection.

The technology provides an automatic water collecting and fire extinguishing tree which can automatically be used for storing rainfall, automatically detecting a forest fire, fire spreading and smog of the forest fire, and automatically spraying water to suppress the forest fire when the forest fireline is reaching. The water collecting and fire extinguishing tree is particularly suitable for areas where forest fires take place frequently and comprises a trunk, a tree crown, a smog and fire detecting system and an automatic fire extinguishing system; the trunk is an airtight hollow column body used for storing water; the tree crown comprises at least one water collector used for collecting rain water; the water collector comprises a leaf and a leaf stem; the leaf is a curved surface which is concave in the middle and high on two sides; the upper end of the leaf stem with a through hole is connected to the edge of the concave part of the leaf; the lower end of the leaf stem penetrates a leaf stem hole in the trunk wall and extends into the internal cavity of the trunk; the water collector is inclined, so that rain water can drip along the two sides of the curved surface to gather in the concave middle part and flows in the trunk through the through hole of the leaf stem; the automatic fire extinguishing system comprises a water pump arranged on a water spray pipe, and a controller for controlling the water pump to work; one end of the water spray pipe extends in the internal cavity of the trunk, and the other end of the water spray pipe is connected with a spray nozzle; and the smog and fire detecting system comprises a smog sensor which is electrically connected with the controller.

The invention discloses a standard molecule for detecting tilletia walkeri castebury&carris;. The standard molecule is a plasmid which can be duplicated, and the plasmid contains at least one of a sequence A (Seq ID N0.1) and a sequence B (Seq ID N0.2); the sequence A is 99.65% homologous with a 2.3kb nucleotide sequence of the mitochondrial DNA of the tilletia walkeri castebury&carris; and the sequence B is 100% homologous with the DNA sequence of a ribosomal internal transcribed spacer of the tilletia walkeri castebury&carris;. The invention also discloses a preparation method and detection method of the standard molecule. The standard molecule constructed in the invention can serve as a positive control for detecting the molecule of the tilletia walkeri castebury&carris; instead of the DNA of the tilletia walkeri castebury&carris; is suitable for qualitative and quantitative PCR (polymerase chain reaction) detection of the tilletia walkeri castebury&carris; and is suitable for the inspection and quarantine department of the port, the agricultural production department, the plant protection department and the like.

The invention discloses plasmid standard molecules PLW03 and PLW04 for detecting Shiga-toxin-producing Escherichia coli and application thereof. The invention particularly discloses a polynucleotide for Shiga-toxin-producing Escherichia coli PCR detection and a primer pair matched with the polynucleotide. The polynucleotide sequence is prepared into the standard plasmid molecules by using an appropriate framework plasmid. When being matched with the primer pair to perform real-time fluorescent PCR detection, the standard plasmid molecules have the advantages of excellent specificity, excellent sensitivity and favorable stability.

The invention discloses a polynucleotide, a method and a kit for detecting Bacillus anthraci, and particularly discloses the polynucleotide which can be used as a standard molecule for real-time fluorescent PCR (polymerase chain reaction) detection of the Bacillus anthraci as well as DNA constructs PA and capA. By the aid of the plasmid standard molecule, the problem of lack of standard substances for real-time fluorescent PCR detection of the Bacillus anthraci is solved, the comparability of the detection result of a real-time fluorescent PCR method of the Bacillus anthraci is guaranteed, and a reliable quality control method is provided for detection with the real-time fluorescent PCR method of the Bacillus anthraci.

The invention discloses C80 concrete prepared from stone chips, the preparation materials of the concrete comprise a cementing material, coarse aggregate, a water reducing agent, water and high-calcium stone chips, the dosage of the cementing material is 580-600kg / m < 3 >, and the water-binder ratio is 0.22-0.26. The C80 concrete has the beneficial effects that the stone chips with the average particle size of less than 4.75 mm are adopted, and stone powder contained in the stone chips can fill cement particle gaps; the gel compactness is improved, the surface energy is relatively low, the dispersion is easy, and the water requirement for achieving the same liquidity is low; the problem of lack of high-quality fly ash can be solved by blending ultrafine stone powder in the stone chips andslag powder to prepare high-performance concrete; river sand is replaced with the stone chips of mine production stone to serve as fine aggregate, the problem that no high-quality river sand is used for preparing high-strength concrete in mountainous areas is solved, environmental protection and sustainable development of building materials are facilitated, and huge economic benefits are achieved.

The invention aims at providing a multifunctional portable moxibustion apparatus system. The system solves the problem of lack of professional moxibustion technicians. A user can select various moxibustion modes at home by himself / herself through the intelligent operation of the moxibustion apparatus system, and a series of moxibustion methods such as hot moxibustion, warm moxibustion and comfortable moxibustion can be implemented by setting time and controlling the temperature and time.

The invention discloses polynucleotide, a method and a kit for real-time fluorescence quantitative PCR (Polymerase Chain Reaction) detection of vibrio cholerae, and particularly provides the polynucleotide, usable for vibrio cholerae PCR detection, and a primer pair matched with the polynucleotide. A polynucleotide sequence is prepared into a standard plasmid molecule PLW07 by using an appropriate framework plasmid, and real-time fluorescence PCR detection is carried out by virtue of the primer pair; the specificity and the sensitivity are extremely high, and the stability is high.

The invention discloses a method for organizing the mixing of spray jet and mainstream gas. By analyzing the mixing mechanism and law of spray jet and mainstream gas, combined with particle dynamics, vortex dynamics and other theories, a blending method based on regulation and control is proposed. The large-scale vortex structure-symmetric anti-vortex pair that dominates the diffusion characteristics of spray droplets in the flow field is used to realize a new idea of ​​rationally organizing the mixing process of the spray jet and the mainstream gas, and according to the characteristics of the symmetrical anti-vortex pair in the mixing flow field The internal relationship between the size and the parameters of the flow field, the determination method of the spray jet atomization characteristic parameters and the nozzle selection method based on the mainstream gas state are given, which solves the problem of mixing the spray jet with the mainstream gas in the current industrial process. The problem of lack of organizational skills in the mixed process.

The invention discloses a positive standard molecule for detecting diaporthe phaseolorum (Cooke etEll) Sacc. var. caulivora Athow et Caldwell (DPC) and diaporthe phaseolorum (Cooke etEll) Sacc. var. meridionalis F.A.Fernandex (DPM). The positive standard molecule is a plasmid molecule capable of autonomously replicating, and a plasmid contains a deoxyribose nucleic acid (DNA) sequence of a riboseinternal transcribed spacer of the DPC and the DPM. The invention further discloses a preparation method and a detection method of the positive standard molecule. The positive standard molecule constructed in the invention can be used for replacing positive DNAs of the DPC and the DPM, and the standard plasmid molecule is suitable for analyzing and detecting the DPC and the DPM through qualitative polymerase chain reaction (PCR) and quantitative PCR. The standard molecule is suitable for being used in the port inspection and quarantine department, the agricultural production department, the plant protection department and the like.

The invention relates to a high-powered recycling process of coal-chemical industry clean wastewater and a special device for the high-powered recycling process. The coal-chemical industry clean wastewater is subjected to multistage clarification, ultrafiltration and osmosis treatment, the water recovery rate of the clean wastewater ultimately reaches above 95%, the purpose of high-powered recycling of the coal-chemical industry clean wastewater is achieved, the water resource is saved and the difficult problem of the deficiency of the fresh water resource is solved.

The invention discloses polynucleotide for staphylococcus aureus detection, a method and a kit and particularly discloses the polynucleotide of standard molecules and capable of being used for real-time fluorescence PCR detection of staphylococcus aureus and a DNA construct LY03. According to the polynucleotide for staphylococcus aureus detection, the method and the kit, plasmid standard molecules solve the problem that standard matter lacks in real-time fluorescence PCR detection of the staphylococcus aureus, the result comparability of the real-time fluorescence PCR method detection of staphylococcus aureus is guaranteed, and a reliable quality control method is provided for real-time fluorescence PCR method detection of staphylococcus aureus.

The invention discloses a method for preparing a Chinese poplar or sycamore or pine wood and plastic composite material by adopting the radiation polymerization, crosslinking and grafting jointly. The method has the following steps of: pre-treatment, monomer impregnation, constant temperature irradiation under the protection of nitrogen and post-treatment and specifically comprises the following steps: (1) preparing the Chinese poplar or sycamore or pine wood serving as the basic material into blanks with appropriate sizes, drying in a drying room, and putting the treated blanks in an impregnating and radiating product box; (2) impregnating by methyl methacrylate, other acrylic acid derivatives and related assistants, introducing protection nitrogen, and increasing the pressure, wherein the impregnation rate of a monomer is 40-170%; (3) radiating at the room temperature under the nitrogen protection, wherein the absorbed dose is 15kGy; and (4) performing aftertreatment: polishing the surface of a radiated wood and plastic composite material to prepare for processing the composite material to floor boards and other products. The fast-growing readily-available low-grade wood such as the Chinese poplar wood is used for preparing the wood and plastic composite material by adopting the radiating, the crosslinking and the grafting, and the wood and plastic composite material has high hardness, good wear resistance, low hygroscopicity and corrosion resistance, can be used for replacing the expensive high-quality wood so as to meet various use requirements.

The invention discloses plasmid standard molecules PLW03 and PLW04 for detecting Shiga-toxin-producing Escherichia coli and application thereof. The invention particularly discloses a polynucleotide for Shiga-toxin-producing Escherichia coli PCR detection and a primer pair matched with the polynucleotide. The polynucleotide sequence is prepared into the standard plasmid molecules by using an appropriate framework plasmid. When being matched with the primer pair to perform real-time fluorescent PCR detection, the standard plasmid molecules have the advantages of excellent specificity, excellent sensitivity and favorable stability.

The invention discloses a standard molecule for wheat tilletia indica mitra detection. The standard molecule is plasmid capable of being replicated, the plasmid contains at least one of sequences A and B, the homology of the A (Seq ID No. 1) and a nucleotide sequence (AF218059) of the last fragment 2.3kb of wheat tilletia indica mitra mitochondria DNA is 99.56 percent, and the homology of the B (Seq ID No. 2) and a DNA sequence (AF399888) of a transcribed spacer in wheat tilletia indica mitra ribosome is 99.85 percent. The invention also discloses a preparation and detection method of the standard molecule. The constructed standard molecule can be used for substituting wheat tilletia indica mitra DNA and used as positive control of wheat tilletia indica mitra molecular detection, is suitable for qualitative polymerase chain reaction (PCR) detection and quantitative PCR detection of wheat tilletia indica mitra, and is suitable for port inspection and quarantine, agricultural production, plant protection and the like.

The invention relates to pickled finger citron. The pickled finger citron is prepared in steps as follows: (1), peeling, shredding and microwave vacuum drying of ripe finger citron; (2), flavoring; (3), fermentation; (4), subpackaging. Pulp of the finger citron is processed according to the comprehensive medicine efficacy of the finger citron and the medicine and food homology concept to form the pickled finger citron, and the problem of lack of finger citron products on the market is solved.

The invention discloses a standard molecule for detecting tilletia walkeri castebury&carris;. The standard molecule is a plasmid which can be duplicated, and the plasmid contains at least one of a sequence A (Seq ID N0.1) and a sequence B (Seq ID N0.2); the sequence A is 99.65% homologous with a 2.3kb nucleotide sequence of the mitochondrial DNA of the tilletia walkeri castebury&carris; and the sequence B is 100% homologous with the DNA sequence of a ribosomal internal transcribed spacer of the tilletia walkeri castebury&carris;. The invention also discloses a preparation method and detection method of the standard molecule. The standard molecule constructed in the invention can serve as a positive control for detecting the molecule of the tilletia walkeri castebury&carris; instead of the DNA of the tilletia walkeri castebury&carris; is suitable for qualitative and quantitative PCR (polymerase chain reaction) detection of the tilletia walkeri castebury&carris; and is suitable for the inspection and quarantine department of the port, the agricultural production department, the plant protection department and the like.