The invention discloses a sterilizing composition containing picoxystrobin and triazole. The sterilizing composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is the picoxystrobin; the active ingredient B is a bactericide selected from any one of triadimenol, bitertanol, diniconazole, hexaconazole, cyproconazole and flutriafol; and the weight ratio of the active ingredient A to the active ingredient B is (0.01-0.6):(0.01-0.4). The composition can prevent and control various crop diseases, has remarkable synergistic effect, enlarges the fungicide spectrum and high activities on powdery mildew, leaf blight, septoria nodorum, rust disease, root rot, smut, anthracnose, brown rot, apple scab, leaf spot, brown spot, Alternaria mali Roberts and leaf rust, reduces using amount of pesticide, reduces residual quantity of the pesticide on the crops, and reduces environmental pollution.