412 results about "Brown spot" patented technology

The invention relates to a synthesis and fungicidal activity of an aryl ether based triazole compound which comprises a structural formula I, the general expression is shown in the figure, in which R represents a hydrogen atom, an alkyl, a methoxyl, a nitro or a halogen. The synthesis and fungicidal activity of an aryl ether based triazole compound has the advantages of strong specificity inhibitory activity to peanut brown spot pathogen at 50ppm to 200ppm and ability to be used for preparing agricultural fungicides.

A comprehensive, single agent cosmetic cream or lotion containing a high number of ingredients that target anti-aging in a defined manner. The cream or lotion contains a high number and variety of active substances that demonstrate excellent safety and efficacy in all of the various defined categories of skin aging, including but not limited to wrinkles, abnormal pigment or brown spots due to aging of the skin and an unexpectedly high efficacy in the reduction of redness and acne and rosacea blemishes.

A comprehensive, single agent cosmetic cream or lotion containing a high number of ingredients that target anti-aging in a defined manner. The cream or lotion contains a high number and variety of active substances that demonstrate excellent safety and efficacy in all of the various defined categories of skin aging, including but not limited to wrinkles, abnormal pigment or brown spots due to aging of the skin and an unexpectedly high efficacy in the reduction of redness and acne and rosacea blemishes.

The invention discloses a streptomyces microflavus. The streptomyces microflavus is the streptomyces microflavus NMG2-4-8, and the preservation number thereof is CGMCG No.3442. The streptomyces microflavus is separated and screened from alfalfa healthy tree rhizosphere soil for the first time in the invention, and experiments prove that the streptomyces microflavus has remarkable antagonistic action for 18 pathogenic fungi and 9 bacteria and broad antibacterial spectrum. The streptomyces microflavus has strong inhabiting action for crop pathogenic bacteria, such as alfalfa downy mildew and the like, shows a good disease prevention effect for the alfalfa downy mildew, powdery mildew, brown spots, stemphylium leaf spot disease, root rot disease and the like, and indicates that the bacterial strain has broad application prospect in the biological prevention and cure fields of alfalfa diseases.

The invention relates to a bactericidal composition containing thifluzamide and triazole compounds. The bactericidal composition comprises an active component A and an active component B, wherein the active component A is selected from thifluzamide, and the active component B is selected from one of metconazole, triticonazole, imibenconazole or fenbuconazole; the weight proportion of the effective active component A to the effective active component B is (1-80 percent): (1-80 percent); and an accessory ingredient and an excipient are added into the effective active components to prepare wettable powder, water dispersible granules, a suspending agent, suspended emulsion or a suspension seed coating agent. The bactericidal composition provided by the invention is used for preventing and curing rice blast, banded sclerotial blight, septoria nodorum, a rust disease, powdery mildew, anthracnose, botrytis, downy mildew, alternaria mali Roberts, a rot disease, a tan disease, cladosporium cucumerinum, leaf spot, an epidemic disease, a black rot disease, a brown spot disease, a scab disease and leaf blight and has the effects of less dosage, rain washing resistance and obvious synergia.

The invention discloses a selective breeding method of a high-resistance tobacco variety and belongs to the technical field of resistive breeding of tobacco. According to the selective breeding method, the tobacco high-yield varieties Yunyan 85, Yunyan 87 and Zhongyan 100 or K326 are taken as recurrent parents, and the cold-resistant variety Honghua Dajinyuan, the drought-resistant variety Zhongyan 14, the brown spot and blackleg resistant variety Zhongyan 86, the root-knot nematode-resistant variety TI1706, the common mosaic virus-resistant variety Taiyan No.7 and the tobacco bacterial wilt-resistant variety D101 as resistant resource parents, and the seven major drought, cold and disease resistant resource genes of the tobacco are pyramided and inserted into the good-quality high-yield tobacco variety with the same genetic background by use of a synchronous backcross and convergent cross method, so that a new high-resistance, high-yield and good-quality tobacco variety, which is highly resistant to 7 drought, cold and disease damage factors and highly consistent with the original recurrent parents in other agricultural and yield traits, is obtained.

The invention belongs to the field of biotechnology and particularly relates to Bacillus subtilis and application thereof. The Bacillus subtilis is Bacillus subtilis HS5B5. The strain is preserved in China General Microbiological Culture Collection Center, with the preservation number of CGMCC No. 6088. The Bacillus subtilis HS5B5 provided by the invention has a very strong restraining effect on the pathogen Cladosporiwm paeoniae of brown spot of peony, and can effectively prevent and cure brown spot of peony. The Bacillus subtilis HS5B5 is harmless to the ecological environment and cannot cause the resistance of the pathogen. The Bacillus subtilis HS5B5 is cultured under simple conditions, is easy to preserve, facilitates industrial production and has a favorable development and application prospect.

The invention provides Bacillus siamensis and application thereof, and belongs to the technical field of microbial prevention and control. Research shows that Bacillus siamensis LZ88 provided by the invention has very good effects on the biological control of plant diseases such as tobacco brown spot, tobacco black shank, wheat scab, grape anthracnose, cucumber fusarium wilt, peanut fusarium and the like, the induction of the resistance to tobacco plant diseases and the like, and provides a new strain resource for biological prevention and control of main diseases of tobacco, such as brown spot, and the like, thereby having good practical application value.

The invention discloses a bacillus firmus strain. The bacillus firmus strain is bacillus firmus Bf-02 CGMCC No.3129, and can prevent and control a plurality of plant diseases, such as the anthracnose of walnuts, the gray mold of cucumber, wheat sharp eyespots, the ring rot of apples, tobacco brown spots, corn southern leaf blight, Dongzao jujube black patch, cotton fusarium wilt, cotton red rot, wheat scab and the like. Tests prove that the zymotic fluid of the bacillus firmus Bf-02 CGMCC No.3129 has remarkable effect of preventing and controlling vegetables, particularly the gray mold of the cucumber with the healthy growth of the vegetables and without hazards, and also remarkable growth promotion and yield increasing effects on the cucumber with the growth rate of 29.95 percent after one-time application.

The invention provides bacillus amyloliquefaciens cd5bc. The strain is classified and named as Bacillus amyloliquefaciens cd5bc and is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO:M 2016232, and the preservation time is April 28, 2016. The invention further provides application of the strain in the aspect of preventing and controlling crop diseases and a biocontrol preparation prepared from the strain. The strain has a very significant preventing and controlling effect on red pepper phytophthora blight, the biocontrol effect reaches up to 100% after inoculation is conducted for 15 days, and the biocontrol effect reaches up to 82.36% after inoculation is conducted for 60 days. The bacillus amyloliquefaciens cd5bc has excellent disease-resistant effects on multiple crop diseases, the bacteriostasis rates of the strain on pathogenic bacteria of red pepper sclerotinia blight, bitter gourd wilt, cucumber wilt, cucumber scabs, rice blast, a rice sheath blight disease, broad bean chocolate spots and broad bean brown spots all reach up to 70% or above, and the bacteriostasis rate of the strain on the broad bean chocolate spots and broad bean brown spots reaches up to 100%.

The invention relates to a bacteriocidal composition containing boscalid and triazole compounds, comprising an active component A and an active component B, wherein, the active component A is selected from boscalid, the active component B is selected from one of imibenconazole, triticonazole, ipconazole or fenbuconazole, and the weight ratio of the active component A to B is 1-80%:1-60%. The composition of the present invention can be prepared into dosage forms, such as wettable powder, water dispersible granule, suspending agent, suspended emulsion, suspending seed coating agent, emulsion in water or micron emulsion, by adding auxiliary agents and excipients into the active components, used for controlling powdery mildew, botrytis, net blotch, rice blast, elsinoe ampelina, septoria nodorum, rust, anthracnose, root rot, rot disease, brown rot, apple scab, leaf spot, epidemic disease, black rot, brown spot, scab and leaf rust, with effects of little chemical consumption, anti-rain wash and obvious synergistic action.

The invention provides a fertilizer for cultivation of tobacco. The fertilizer consists of the components by weight as follows: 0.5-1.5 parts of a composite microbial agent, 3-7 parts of an activating agent, 3-7 parts of binder, and 86-92 parts of nitrogen, phosphorus and potassium fertilizer. The effective components of the composite microbial agent are Bacillus licheniformis, Bacillus pumilus, Brevibacillus laterosporus, Bacillus megatherium and Silicate bacteria. The invention further provides a preparation method and an application of the fertilizer. The product provided by the invention can remarkably improve crumb-structure of soil for cultivation of tobacco, and microflora distribution and micro-environment of the soil. A plenty of derivatives are generated, thereby chelating or blocking damage of heavy metals, and absorption and assimilation of tobacco to nutrient elements are improved, the drought resisting and disease resisting abilities of tobacco are enhanced, damage of diseases and insects caused by continuous cropping of tobacco in successive years are effectively prevented, and in particular, tobacco mosaic virus and brown spot disease are specially prevented and treated. According to the invention, the raw materials are low in cost and easy to obtain and the preparation and using methods are simple and easy to operate.

Citrus canker and brown spot are common diseases of citrus. At present, few drugs are available to prevent and control the two diseases and have certain defects. Amino groups at the 7th positions of fluoroquinolone drugs are linked with an active fragment by means of a connecting structure so as to obtain compounds shown in a formula I or a formula II. Experiments prove that the compounds providedby the invention have effects of preventing and controlling the citrus canker and the brown spot and have very good application prospect.

The invention discloses a bamboo dice online detection method based on vision. The method includes the following steps that 1, bamboo dices to be detected move through a second conveyor belt, and an image triggering and collecting module carries out photographing to collect images and numbers the images; 2, the images are transmitted to a PC for white balance processing so that images according with human vision can be obtained, then whether defects exist in the images of the bamboo dices to be detected or not is judged according to a bamboo dice defect detection algorithm, and defective bamboo dices to be detected are removed, wherein the detection process specifically includes the steps of outline defect detection, white spot detection and brown spot detection. According to the method, the bamboo dices can be automatically conveyed and automatically turned over, image information of the bamboo dices to be detected is collected through the image triggering and collecting module, the appearance defect, the white spot defect and the brown spot defect of the bamboo dices are detected and judged on line through the PC according to the defined bamboo dice defect detection algorithm, and the defective bamboo dices are removed; the manual picking cost is significantly reduced, the bamboo dice detection efficiency is improved, the product percent of pass is increased, input is small, operation is convenient, and the method has broad market prospects.

The invention discloses a method for detecting the 4-methyl imidazole in food quickly belonging to food additives detection range. The sample containing caramel pigment is extracted in solid phase through solid phase extraction pile (XTR is more than 500mg), after the sample passes through the pile, it is rinsed with organic solvent, the purged liquid is condensed, uses micro sample applicator to spot sample directly on the chromatography material polyamide film, and it is developed in the developing agent with the same proportion, observes it with naked eye after it is color developed with iodine steam, it observes the brown spots with the rf values the same to the standard sample in rf range between 0.5 and 0.7, then considers that the measured material has 4-methyl imidazole. It can detect if the measured sample is natural or is confected manually, especially proper to detect the sample such as sauce, vinegar and cola containing caramel pigment.

The invention provides acid-resistant pseudomonas protegens CLP-6 and application thereof. According to the acid-resistant pseudomonas protegens CLP-6 and the application thereof, the bacterial strainof the pseudomonas protegens CLP-6 is preserved in China General Microbiological Culture Collection Center on October 27th, 2016, and the biological preservation number of the pseudomonas protegens CLP-6 is CGMCC No.13205; the bacterial strain of the pseudomonas protegens CLP-6 has acid resistance, is good in disease prevention and plant growth promotion effects and high in stress resistance, hasthe antagonistic activity of pathogenic bacteria, is wide in soil acidity tolerance range and wide in antibacterial spectrum, and has application and development potential in prevention and treatmentof bacterial wilt, black shanks and brown spots; and meanwhile, under the general background that the acidification conditions in China is increasingly severe, the research on the bacterial strain ofthe pseudomonas protegens CLP-6 has reference significance in prevention and treatment of plant diseases under various soil qualities, a microbial resource is provided for green prevention and control over the bacterial wilt, the black shanks and the brown spots in acid soil, and secondary metabolites of microorganisms are excavated, so that an important foundation can be laid for the biocontrolefficacy on the bacterial wilt, the black shanks and the brown spots in the acid soil.

The invention provides an agricultural fungicide WYLZ-1349 taking azalomycin F3, azalomycin F4 and a nyphimycin composition as effective constituents, as well as preparation method and application thereof. The preparation method of the agricultural fungicide WYLZ-1349 comprises the following steps of: firstly culturing Streptomyces malaysiensis ECO 00002 with the preservation number of CGMCCNo. 1680; then separately extracting a fermentation culture; breaking the wall of mycelium with acetone or methanol and mixing with a fermentation supernate; and absorbing by macroporous absorption resin for separation; eluting by the acetone or the methanol; concentrating or further purifying an eluent so as to obtain the agricultural fungicide WYLZ-1349 or the azalomycin F3, the azalomycin F4 and nyphimycin; and then adding a carrier or an auxiliary agent so as to obtain a finished product. Test results show that the agricultural fungicide WYLZ-1349 has good effect especially in preventing and controlling rice blast, tobacco brown spot, tomato gray mold and pepper anthracnose and has small using amount, low using cost for peasants, wide usable range, and the like compared with the traditional medicament.

Disclosed is the agricultural pesticide containing camptothecine or camptothecine derivatives and the use thereof, wherein the pesticide comprises camptothecine or its derivatives 0.02-2.0%, emulsifying agent 1.0-20%,penetrating agent 0.5-10%, and balancing organic solvent. The pesticide can be used for controlling agronomic crop downy mildew, leaf streak, pythium blight, head blight, root and stem rot, rhizoctonia rot, damping-off, wilt,bitter rot, powdery mildew, black shank, brown spot, leaf scab, ring rot, black pox, gray mold,phytophthora rot, ulcer disease, black spot, bacterial wilt, angular leaf spot, leaf streak and leaf scar.

The invention discloses a biocontrol bacterium and azoxystrobin composition belonging to the technical field of bactericides. The biocontrol bacterium and azoxystrobin composition contains active components including bacillus subtilis and azoxystrobin. The biocontrol bacterium and azoxystrobin composition disclosed by the invention can be used for remarkably reducing the medication cost while effectively preventing and controlling various diseases such as rice sheath blight disease, rice false smut, rice blast, ear rot, seedling-stage damping-off, tomato late blight, gray mold, wheat powdery mildew, sheath blight, cotton rhizoctoniosis, foxysporum, verticillium dahliae, soybean root rot, corn sheath blight, cucumber gray mold, blight, tobacco brown spot, bacterial wilt, various field crop and garden crop root rots and damping off, so that the medication cost can be reduced, the preventing and controlling ranges can be widened, the environment pollution can be reduced, and the phytotoxicity and pesticide resistance risks can be reduced.

The invention relates to a phytoplankton culture method, belonging to the field of fresh water plant culture. The method for culturing high-density fresh water attached diatom is characterized by comprising the following steps: preparation of feed rotten liquid: by using a common fresh water fish compound feed, adding 3-5 times by volume of tap water, and carrying out rotting fermentation until the odor becomes weak; adding tap water and the feed rotten liquid into a glass container, wherein larger particles are filtered out before adding the feed rotten liquid; putting the glass container in a glass greenhouse or in an outdoor place under solar radiation (keeping out rain in rainy days), and ensuring the lighting condition to be solar radiation intensity of winter and spring; and heating the water body in the glass container to keep the temperature of the water body at 20-30 DEG C while aerating the water body for 10-14 days, wherein red brown spots, which are diatom, can grow on the wall of the glass container.

The invention relates to a method for constructing a brown spotted rockfish fin cell line. The method is as follows: brown spotted rockfish fin tissue are taken as a material, the hyaluronidase with the concentration of 0.5% and collagenase II with the concentration of 0.2% combining digestion method is used for initiating primary culture, and the material is cultured in a DMEM / F12 culture medium which has the PH value of 7.0-7.4 and contains fetal calf serum, alkaline fibroblast growth factor, insulin-like growth factor I and carboxymethyl chito-oligosacaride, and the trypsin digestion method is adopted for subculturing. The brown spotted rockfish fin cell line constructed by the method is passed to the sixty-first generation at present. The method has scientific and reasonable process, and is expected to be applied to separating, identifying and reproducing fish viruses, preparing viral vaccines, studying the interaction between viruses and host cells, etc.

The invention discloses a health-care bitter gourd tea bag, which is prepared by using bitter gourds, shelled Chinese waxgourd seeds, orange peel, peach blossom and honey as raw materials and by drying, crushing, mixing, disinfecting and sterilizing and packaging. The health-care bitter gourd tea bag has the effects of losing weight, removing toxins, beautifying the skin, activating blood and benefiting vital energy, and can prevent the dryness and ageing of the skin, improve the vitality and elasticity of the skin, and effectively prevent yellow brown spots, freckles and black spots; and when a user drinks the health-care bitter gourd tea regularly, the effect of making appearance red and bright, skin delicate and soft and hair dark and bright is achieved.

The invention discloses a high-yield cultivation method of interplanting of pinellia ternata in apple orchards, belonging to the technical field of interplanting cultivation. The high-yield cultivation method of interplanting of pinellia ternata in apple orchards has the following beneficial effects: apple trees are utilized to shield pinellia ternate from intense light such that pinellia ternate is protected against sprout tumble or death due to exposure under intense light, thereby decreasing production cost; gaps among apple trees are fully utilized so that land resources are saved; meanwhile, fertilizers and water resources in the process of growing pinellia ternate are utilized; without additional fertilizers and water nutrients, the nutrition demand during the growth of apple trees is met; as a result, yield of apples is increased and the incidence rate of brown spots and rotten diseases during the process of planting apples is reduced; yield of pinellia ternate is greatly improved; and the content of needle-like calcium oxalate crystal with stimulating components in pinellia ternata is also decreased, thereby ensuring medical security of pinellia ternate to the large extent.

The invention discloses a biocontrol bacterium and pyraclostrobin composition belonging to the technical field of bactericides. The biocontrol bacterium and pyraclostrobin composition contains active components including bacillus subtilis and pyraclostrobin. The biocontrol bacterium and pyraclostrobin composition disclosed by the invention can be used for remarkably reducing the medication cost while effectively preventing and controlling various diseases such as rice sheath blight disease, rice false smut, rice blast, ear rot, seedling-stage damping-off, tomato late blight, gray mold, wheat powdery mildew, sheath blight, cotton rhizoctoniosis, foxysporum, verticillium dahliae, soybean root rot, corn sheath blight, cucumber gray mold, blight, tobacco brown spot, bacterial wilt, various field crop and garden crop root rots and damping off, so that the medication cost can be reduced, the preventing and controlling ranges can be widened, the environment pollution can be reduced, and the phytotoxicity and pesticide resistance risks can be reduced.

The present invention relates to a bactericidal composition containing probenazole, which includes an active ingredient A and an active ingredient B, wherein the active ingredient A is probenazole, the active component B is one selected from the group consisting of carbendazim, thiophanate-methyl, fosetyl-aluminum, prochloraz, mancozeb, propineb, and polyram, and the weight ratio of the active component A and B is 1:80 to 80:1. The effective active ingredients are added with additives to prepare a wettable powder, a water dispersible granule, a suspending agent or a suspoemulsion. The composition is used for the control of rice blast, bacterial leaf blight, sheath blight, anthracnose, ring rot, brown spot disease, leaf spot disease, gibberellic disease, downy mildew, epidemic disease, powdery mildew, bakanae disease, penicilliosis, early blight, late blight, citrus scab, alternaria leaf spot, rust disease and scab, is resistant to rain wash and has obvious synergism.

The invention discloses a bactericidal composition containing pyraclostrobin and prothioconazole and application thereof. The bactericidal composition uses the pyraclostrobin and prothioconazole as main effective components, wherein the weight ratio of the pyraclostrobin to the prothioconazole is (1-70):(1-50). The bactericidal composition can be used for preventing and treating diseases on cereals, melons and fruits, or vegetable crops, has higher synergic effect, overcomes and postpones the drug resistance of pathogenic bacteria, has the advantages of high bactericidal speed and long duration time, lowers the use cost, and has obviously better preventing and treating effects than a single agent. The bactericidal composition can be used for preventing and treating fungus diseases on the crops, especially can be used for preventing and treating leaf blight, rust disease, powdery mildew, downy mildew, epidemic disease, anthracnose, scab, brown spot, seedling blight, gray mold, banana freckle, penicilliosis and other fungus diseases, and has better effect than a single agent.

The invention discloses a purple-mouth tea-dust glaze and a method for preparing a purple-mouth tea-dust glaze product. The purple-mouth tea-dust glaze comprises the following components in percentage by weight: 22-35 percent of albite, 10-15 percent of calcium carbonate, 5-8 percent of talc, 2-3 percent of kaoline, 7-8 percent of ferric oxide, 1-2 percent of nickel dioxide, 3-5 percent of magnesium carbonate and the balance of quartz. The method comprises glaze preparation and product preparation, wherein in the product preparation, the steps of blank preparation, feeding and burning, cooling and the like adopt strict temperature control conditions and a uniform glazing method. A ceramic product which is prepared by adopting the prescription and burning process of the purple-mouth tea-dust glaze has unique properties and characteristics, and is yellow and smooth in appearance glaze, has block or black-brown spots; the glaze is doped by yellow and green colors, and looks like tea dusts, and the mouth is provided with a 'purple-mouth edge'; the burning process is convenient to control and is energy-saving, reliability can be realized by virtue of burning temperature control, and the yield of burnt products is high.

The invention discloses a method for detecting the titre of live viruses, which comprises the following steps: firstly, infecting diploid cells or other adherent cells with dilute viruses to be detected, culturing and fixing the infected cells; secondly, adding virus antiserum (first antibody) into the fixed cells, culturing and washing, adding a second antibody marked by horseradish peroxidase (HRP), culturing and washing, adding coloring solution for color development and producing red or brown spots, or adding virus antiserum marked by the HRP into the fixed cells, culturing the virus antiserum and washing, adding the coloring solution for color development and producing red or brown spots; thirdly, calculating the virus titre according to the quantity of the red or brown spots and the dilution multiple, or calculating the 1gTCID50 value of the viruses according to the dilution degree and the hole numbers of the spots. The detection method can be applicable to the titre detection of various live viruses, can shorten the detection time of the titre of the viruses due to fast detection speed, has good specificity as the immune spots are caused by special immune reaction, low detection cost as well as simple and convenient operation and does not need special instruments of optical or fluorescent microscopes, and the like or other reagents.