322 results about "Rhizoctonia" patented technology

The invention discloses a streptomyces corchorusii strain NF0919, which is collected in China General Microbiological Culture Collection Center, and has the collection number CGMCC No. 3968. The strain can be used for preventing and treating rhizoctonia cerealis, rice false smut, rice sheath blight disease and other various plant diseases.

The invention relates to a Bacillus amyloliquefaciense BA-KA3 and application thereof. The Bacillus amyloliquefaciense BA-KA3 is preserved in China General Microbiological Culture Collection Center on October 23, 2013, and has preservation No. CGMCC8342; its on tomato gray mold germs has obviously of antagonistic anti-role, on wheat scab bacteria. The Bacillus amyloliquefaciense BA-KA3 has obvious antagonistic effect on Fusarium graminearum, Rhizoctonia cerealis, Alternaria alternata, Phytophthora capsici, Colletotrichum capsici, Cercospora personata, Alternaria alternate and Garlic sclerotinia disease, and can significantly improve plant growth indexes such as germination rate, and budding rate and root length of seeds,and especially applies to preparation of agricultural preparations for control of tomato diseases due to Botrytiscinerea invasion and has certain growth promotion effect.

The present invention relates to an application of anthraquinone derivative as pesticice for controlling plant fungous diseases. The anthraquinone derivative pesticide contains one kind or several kinds of 10 anthraquinone derivative compounds. The anthraquinone derivative compounds have obvious action for preventing and curing several plant diseases, and can be used for preventing and curing economic crop, grain crop, vegetable, fruit tree and melon diseases due to fungi, including sphaerotheca, erysiphe, blumeria, fusarium, sclerotinia, pyricularia, rhizoctonia, colletotrichum, botrytis, verticillium and alternaria.

The invention discloses bacillus subtilis S001 capable of inhibiting a variety of pathogens and further discloses a microbial preparation prepared from the bacillus subtilis S001 and a preparation method the microbial preparation. The bacillus subtilis S001 with the collection number of CGMCC No.6425 has the function of promoting growth and inhibiting pathogens including the fusarium, pepper rhizoctonia solani, tomato leaf mold, tomato early blight pathogen, tomato grey mold, eggplant sclerotia pathogen, wheat full-rotting pathogen, wheat root rotting pathogen and wheat sheath blight pathogen. The microbial pesticides and microbial fertilizers prepared from the bacillus subtilis S001 are environmentally-friendly and have good safety, and pathogens can not resist the microbial pesticides and the microbial fertilizers easily.

Disclosed is a bio-fertilizer composition for promoting growth of orchid plants. The composition comprises a symbiotic organism, a growth substance; and a medium, which is used to mix and evenly disperse the symbiotic organisms and the growth substance. The symbiotic organism is selected from the group consisting of Rhizoctonia sp. (BCRC930076) and Rhizoctonia sp. (BCRC930077). The bio-fertilizer composition according to the invention can promote the growth of orchid plants, increase the flowering rate and flowering quality, decrease the occurrence of root disease to prevent the usage of pesticide, and increase the beneficial components toward human health of medicinal orchids.

The invention relates to an organic liquid fertilizer containing chitosan. The organic liquid fertilizer comprises the chitosan, humic acid, fulvic acid, phosphorus pentoxide, potassium hydroxide, boric acid, edetic acid, zinc sulphate, manganese sulfate monohydrate, glycine, glutamic acid, serine and lysine. Aqueous extract of traditional Chinese medicines is added into the organic liquid fertilizer. As various marine bioactive ingredients such as the chitosan are added into the organic liquid fertilizer, the organic liquid fertilizer can be used for culturing a great quantity of effective microbial communities of actinomycetes and the like within short time after being applied to soil. Antibiotics and other substances secreted from the effective microbial communities can inhibit harmful microbial communities of pythium aphanidermatum, rhizoctonia and the like, and thus a microbial environment of the soil can be improved rapidly. The chitosan also can be used for accelerating the growth of root systems and the combination of the root systems and the soil. In addition, as the aqueous extract with insecticidal efficacy of the traditional Chinese medicines is added into the organic liquid fertilizer, plant diseases and insect pests of leaf surfaces can be effectively reduced when the fertilizer is applied to the leaf surfaces.

A chemically-biological synergistic bactericide for preventing and treating the Rhizoctonia diseases and fungal diseases of agricultural crops contains jinggangmycin A (validamycin A), kresoxim-methyl, filler, solvent, water and assistant.

Disclosed herein is a method for increasing the production of crops, particularly wheat and soybean, using herbicide resistant cultivars. In one aspect of this method, the method increases crop yield by diminishing the impact of the root diseases caused by Gaeumannomyces and Rhizoctonia species by treating the crop with an herbicide, in particular glyphosate. In another aspect the method for treating crops reduces the effects foliar pathogens and diseases, particularly fungal pathogens, such as rusts, including soybean rust, stem rust, stripe rust and leaf rust.

The invention provides a bacillus amyloliquefaciens ZCM18 with broad spectrum growth-promoting and stress-resisting effects. The preservation No. of the bacillus amyloliquefaciens ZCM18 is CGMCC No.12959. The bacterial strain has higher phosphate solubilization and nitrogen fixation capability and capability of generating IAA, ACCD, siderophore and cytokinin; the tolerance of salinity can reach up to 5%; the bacillus amyloliquefaciens has higher antagonism to fusarium and rhizoctonia pathogenic bacteria and is suitable for the production of multifunctional bio-fertilizer, such as, disease prevention, growth promoting and salt and alkali resistance; the application scope is wide; the bacillus amyloliquefaciens is suitable for biological improvement of various soil (saline-alkali soil, degenerated soil and conventional cultivated land) and production of various plants (vegetables, grain crops and fruit trees); the bacillus amyloliquefaciens has obvious effects in boosting the root development, preventing and controlling root diseases, increasing the crop yield, and the like; the bacillus amyloliquefaciens is capable of reducing the dosage of chemical fertilizers and pesticides and is wide in application prospect.

The invention discloses Burkholderia pyrrocinia of which the classification name is Burkholderia pyrrocinia. The strain number is LWK2, the Burkholderia pyrrocinia is preserved in the China center for type culture collection (CCTCC), the preservation number is CCTCC NO: M 2016007, and the preservation date is January 5, 2016. The invention further discloses application of the Burkholderia pyrrocinia in cercidiphyllum japonicum growth promotion. The LWK2 strain has the strong potassium decomposing capacity and auxin secretion capacity and can obviously promote germination of cucumber seeds; when an LWK2 bactericide is inoculated to a cercidiphyllum japonicum seedling, the result shows that the bactericide can obviously promote growth of the cercidiphyllum japonicum seedling. Meanwhile, the strain has a certain antagonistic effect on phytopathogen such as pyticularia oryzae, cytospora chrysosperma, fusarium oxysporum, riziocotinia solani and rhizoctonia.

The invention relates to Bacillus amyloliquefaciens LX-J1 and application thereof. A preparation of the Bacillus amyloliquefaciens LX-J1 is prepared from a fermentation solution, copper sulfate, a phosphorus compound, a potassium compound and xanthan gum. Compared with the prior art, the liquid preparation of Bacillus amyloliquefaciens LX-J1 disclosed by the invention has the advantages that the inhibition ratio for rhizoctonia cerealis is up to 87.5%, the inhibition ratio for rhizoctonia solani is up to 78.82%, the inhibition ratio for pythium ultinum trow is up to 87%, the inhibition ratio for sclerotium rolfsii is up to 80.67%, the inhibition ratio for potato black scurf is up to 78.57%, and the inhibition ratio for corticium rolfsii saccardo can be up to more than 83%.

The invention provides a sterilization composition comprising the following active components with effective quantity: flutolanil and one of triazole class compounds of hexaconaole, ethyltrianol, diniconazole, triazolone, triadimenol, epoxiconazole, fluquinconazole, flusilazole, systhane, penconazole, tetraconazole, biphenyl triadimenol and cyclopropyl hexaconazole. The composition can be used forpreventing and treating diseases caused by rhizoctonia and has the following advantages: (1) mixed matching has an obvious synergistic action, and the preventing and treating effect is enhanced; (2)the function mechanisms of two active components in the composition are different, the single selection pressure of pathogenic bacteria is lightened, and the composition is beneficial to overcoming the generation of the drug resistance of the pathogenic bacteria and delaying the generation speed of resistibility; (3) the preventing effect is obviously enhanced after the medicaments are mixed and matched, and the use quantity of the effective components is reduced, thereby the cost is lowered and environmental pollution is lightened; and (4) preventing and treating spectra are expanded, the composition also has a treating function to other diseases, and can treat multiple diseases.

The invention provides a bacterial biological fungicide and a preparation method thereof. Microbial pesticide living Pseudomonas putida is used as a starting strain; and the bacterial biological fungicide is obtained after fermentation and reproduction. The bacterial biological fungicide can be used for nearly all crops infected by Pythium, Rhizoctonia and Fusarium, is safe for the crops, and has good effect on the crops such as cucumber, tomato, scallion, eggplant, green pepper, cotton, watermelon, sesame and the like. The bacterial biological fungicide has the greatest advantages that the bacterial biological fungicide is all nature, harmless, safe to people and livestock and environment-friendly; the pathogenic bacteria are not easy to produce resistance; and the bacterial biological fungicide is favorable for production of green vegetables, and has huge economic, ecological and social benefits.

The invention discloses a PCR (polymerase chain reaction) primer capable of simultaneously detecting five soil-borne fungal diseases of wheat paddock, wherein the sequence of the rhizoctonia solani primer is as follows: an upstream primer P1: 5'-TCCTCCCGCTTATTGATATGC-3'; and a downstream primer P2: 5'-CGGTTGAGCTGGGTCTTTTA-3'. A multi-PCR detecting system built by the invention can simultaneously detect the five pathogenic funguses such as rhizoctonia cerealis, gaeumannomyces graminis, fusarium graminearum, fake fusarium graminearum and bipolaris sorokinianum. The size of an amplified product segment disclosed by the invention is accordant with an expected result, and five pairs of the primers only can be generated aiming at the target specific stripe and the non-specific stripe, so that the five pathogenic funguses can be well distinguished from one another. The PCR primer is convenient and quick: a sample can be prepared and detected within four hours, and the five pathogens such as the rhizoctonia cerealis, the gaeumannomyces graminis, the fusarium graminearum, the fake fusarium graminearum and the bipolaris sorokinianum can be simultaneously detected; the PCR primer is high in sensitivity: 0.39ng of a pathogen DNA (deoxyribonucleic acid) sample can be detected: and the PCR primer is exact: compared with the conventional separation identifying and symptom identifying method,the PCR primer is higher in the accuracy of the detecting result.

The invention relates to bacillus velezensis having various disease preventive effects, application thereof and a biocontrol inoculant and belongs to the technical field of plant disease and pest prevention and control. Bacillus velezensis MS012 having bacteriostatic activity is obtained by separating and screening from healthy branches of malus sieversii. Bacillus amyloliquefaciens has remarkableantagonistic effect on botryosphaeria dothidea, colletotrichum gloeosporioides, phomopsis mali, rhizoctonia cerealis, gaeumannomyces graminis, botrytis cinerea, fusarium graminerum, clivia stem basaldecay pathogen, nandina anthrax pathogen and erwinia amylovora and has broad spectra in bacteriostatic object, and a new microbial resource is provided for biocontrol on disease and pest of plants like apple trees.

The invention discloses ectomycorrhizal helper bacteria bacillus pumilus HR10. The classified nomenclature of the ectomycorrhizal helper bacteria bacillus pumilus HR10 is bacillus pumilus, the bacillus pumilus is preserved in CCTCC, the collection number of the bacillus pumilus is CCTCC No: M 2010143 and the preservation date is June 12th, 2010. The bacillus pumilus HR10 is plant growth-promoting rhizobacteria, has direct promoting effect on black tree growth and can promote the growth of rhizipogen luteous of ectomycorrhizal fungi; double inoculation of the bacillus pumilus HR10 and the rhizipogen luteous has obvious growth-promoting effect on pinus thunbergii parl; the bacillus pumilus HR10 has inhibiting effect on pathogenic rhizoctonia of pine nursery stock damping-off disease; and greenhouse preventing and controlling experiments show that the bacillus pumilus HR10 strains can effectively prevent and control the pine nursery stock damping-off disease.

The invention discloses a synergistic fungicide composition, the active components of which are flutolanil and any strobilurin fungicide, and the weight proportion between the flutolanil and the strobilurin fungicide is 50:1 to 1:50. The strobilurin fungicide is chosen from azoxystrobin, kresoxim methyl, SYP-1620, trifloxystrobin, enestroburin, ZJ0712, pyraclostrobin and the like. The composition can be used to control diseases caused by rhizoctonia, and in particular, the composition has a good control effect on rice sheath blight.

The invention belongs to the field of agricultural biological control and discloses a strain HMB27703 of bacillus subtilis for controlling cotton rhizoctonia. The collection number of the strain is CGMCC No.11458. The invention further discloses a microbial agent for preparation of the bacillus subtilis and an application of the microbial agent for control of the cotton rhizoctonia. The bacillus subtilis HBM27703 has a high pesticide effect on cotton rhizoctonia, and the average control effect is higher than 80.0%; besides, the bacteriocidal spectrum is wide, and bacillus subtilis has a remarkable control effect on botrytis cinerea and cotton fusarium wilt besides cotton rhizoctonia; moreover, bacillus subtilis has high pertinency, the efficacy lasts long, and pesticide resistance is not prone to be caused; the microbial agent is safe to human and the livestock and causes little environmental pollution; the preparation method is simple, low in cost and simple to use.

The invention provides crop growth promoting antagonistic trichoderma, in particular to Trichoderma atroviride H18-1-1 and TF28 with preservation numbers of CGMCC No.9774 and 9775 and aims to solve the problem that using agricultural chemicals for preventing and treating agricultural diseases is prone to causing environment pollution and agricultural chemical residues, jeopardizing an agricultural ecological system, killing beneficial microorganisms in the environment, improving drug resistance of phytopathogen and finally causing decline of prevention and treatment effects and even failure in prevention and treatment. The trichoderma not only can be applied to agricultural biological prevention and treatment but also can promote growth of crops such as wheat and prevent and treat diseases caused by rhizoctonia cerealis, fusarium culmorum, fusarium proliferatum and / or fusarium oxysporum and the like.

Disclosed is the agricultural pesticide containing camptothecine or camptothecine derivatives and the use thereof, wherein the pesticide comprises camptothecine or its derivatives 0.02-2.0%, emulsifying agent 1.0-20%,penetrating agent 0.5-10%, and balancing organic solvent. The pesticide can be used for controlling agronomic crop downy mildew, leaf streak, pythium blight, head blight, root and stem rot, rhizoctonia rot, damping-off, wilt,bitter rot, powdery mildew, black shank, brown spot, leaf scab, ring rot, black pox, gray mold,phytophthora rot, ulcer disease, black spot, bacterial wilt, angular leaf spot, leaf streak and leaf scar.

The invention relates to antagonistic bacteria preventing and removing continuous cropping cucumber rhizoctonia rot and a microbial organic fertilizer produced thereby, which belong to the technologies of agricultural intensified production. The antagonistic bacteria NJN43 with the significant antagonistic effect for original fungi of the cucumber rhizoctonia rot is obtained by separation, the antagonistic bacteria and an organic compost are mixed for preparing the microbial organic fertilizer, the fertilizer contains more than 1 multiplied by 10<8> / g of antagonistic microbes, the content of total nitrogen is 4-5% (more than 90% is organic nitrogen), the total content of nutrients of nitrogen, phosphorus and potassium is 6-10% and the content of organic matters is 30-35%. Tests show that the microbial organic fertilizer can lead the antagonistic bacteria to be rapidly propagated after being applied into the soil and form heterotic groups in the soil, and the biological rate for preventing and treating the rhizoctonia rot in the continuous cropping soil with the serious cucumber rhizoctonia rot can be more than 70%. The long-term application of the microbial organic fertilizer in the soil without the formation of continuous cropping obstacles can prevent the occurrence of the rhizoctonia rot of continuous cropping crops to a great extent.

The invention discloses bacillus amyloliquefaciens. Bacillus amyloliquefaciens has a conservation number of CGMCC No.13492. Bacillus amyloliquefaciens is used for inhibiting bacteria, molds and phytopathogen; the bacteria are escherichia coli, staphylococcus, tetrads and bacillus subtilis; the molds are mucor, aspergillus niger and penicillium; phytopathogen is rhizoctonia cereal, lily root rot, fusarium oxysporum vasinfectum, fusarium oxyspirum F sp niveum, colletotrichum capsici, botrytis cinerea, apple ring spot and peach brown rot.

The present invention relates to a microbial bacteriocide, its preparation method and application. Said invented production strain is Brevibacillus laterosporus G4. Said invented microbial bacteriocide is prepared by adopting conventional shake-flask fermentation process, and its solid culture medium formula includes beef extract 3g, peptone 8g, sodium chloride 4g, agar 18g and water 1000 ml, pH 7.5 and its liquid fermentation culture medium formula contains 0.2-0.4% of beef extract, 10-20% of soybean care powder, 0.5-2% of fish powder, 0.3-0.5% of sodium chloride, pH 7-8 and the rest is water. Said invention has good bactericidal action for two plant pathogenic bacteria of fusarium and rhizoctonia.

The invention relates to a microbial preparation for preventing and controlling plant silborne fungal diseases, and application thereof, which belong to the field of microbial pesticide. The microbial preparation is prepared by a conventional method. A production strain of the microbial preparation is actinomyces Da07210 which is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the actinomyces Da07210 is CGMCC No.2561. The strain is prepared into a liquid preparation through liquid fermentation, or is prepared into wettable powder by mixing the strain with an adsorption carrier according to a certain proportion, filtering, concentrating, grinding and airing the mixture. The microbial preparation has the advantages that the microbial preparation can prevent and control banana wilt, plague, seedling blight, damping-off and the like caused by fusarium, phytophthora, rhizoctonia and pythium pathogenic fungi, and can also stimulate root systems of plants to grow and promote the growth of plant seedlings.

The invention relates to a bacterial strain WXX-2 and a bacterial agent for controlling peanut root rot. The bacterial strain is Bacillus pumilus with the preservation number of CGMCC No. 11540. The 16s rRNA base sequence of the bacterial strain is shown as SEQ ID No.1. Chitinase produced by the bacterial strain and used for degrading fungal hypha cell walls has higher activity ad has significant degradation capacity against fungal pathogen hyphae such as fusarium oxysporum, fusarium solani, blight bacteria, rhizoctonia and the like infecting roots of crops. The quantity of seed dressing with the bacterial agent is generally only 10-15 kg / mu, damage by the frequent peanut root rot in upland red soil can be effectively controlled, the control effect reaches 60% or higher, and the bacterial strain WXX-2 and the bacterial agent have the advantages of small application quantity, broad antibacterial spectrum, remarkable yield increasing effect and the like, and the influence of the root rot on the loss of peanut yield is effectively reduced.

The invention provides Bacillus amyloliquefaciens F028. The strain is preserved in the China General Microbiological Culture Collection Center on April 20, 2020, and has a preservation registration number of CGMCC No.19590. A biological control preparation prepared from the strain has a broad-spectrum antibacterial effect on bacterial diseases caused by Xanthomonas campestris, Erwiniacarotovora and Ralstonia solanacearum, and fungal diseases caused by Botrytis cinerea, Rhizoctonia solani, AlternariaNees, Fusarium, Phytophthora spp, Glomerellacingulata(Stonem.)Spauld et Schrenk, Colletotrichumcapsici and Beauveria bassiana. According to the invention, a new resource is provided for replacing a chemically synthesized bactericide with microorganisms and preventing and controlling plant diseases by using a biological prevention and control method, and the Bacillus amyloliquefaciens can be developed and utilized as a biopesticide; and moreover, the Bacillus amyloliquefaciens provided by the invention has a promotion effect on the plant growth of tomatoes and tobaccos, and has a wide application prospect.

The invention relates to a synthesis method of 5, 7, 4'-trihydroxy-3', 5'-dimethoxyflavone and application thereof, particularly to a chemical synthesis method of allelochemicals, 5, 7, 4'-trihydroxy-3', 5'-dimethoxyflavone, by separating from allelochemical rice, Oryza sativa L.(PI312777). Syringic acid and phloroglucinol are used as the raw materials, the object products are synthesized by four steps. The products are protected by benzyl, and benzyl is synchronously removed with glacial acetic acid heating and ring closing in the final reaction step, thereby reducing the reaction steps. Compared with the method of removing benzyl by Pd / C charging hydrogen, the method of the invention is more simple, economical, and safe. The invention is capable of effectively stopping growth of garnyardgrass, cyperus iria linn and cyperus difformis linn seeds in rice, restraining paddyfield weed, improving rice yield, stopping spore germination of pyricularia oryzae and rhizoctonia solan so as to avoid the problems due to pesticide application and promote sustainable development of agriculture.

The invention discloses antagonistic actinomycete for preventing and controlling continuously cropped strawberry root rot and a preparation method of a biocontrol agent of the antagonistic actinomycete. Bacterial strain B04 belongs to streptomyces hygroscopicus (Streptomyces hygroscopicus), with the strain preservation number of CGMCC NO.9918. The biocontrol strain B04 is separated from a healthy strawberry root, and has a strong inhibiting effect on pathogenic bacteria, such as fusarium, verticillium dahliae, rhizoctonia, phytophthora and the like, causing strawberry soil-borne root diseases. The antagonistic actinomycete prepared by taking wormcast and wheat bran as the main materials of fermentation medium can completely meet the nutritional requirements produced by solid fermentation of biocontrol antinomycetes. Compared with conventional fermentation, the production cost of the preparation method is reduced by 30 to 50 percent, and the preparation method is beneficial for popularization and application of the biocontrol agent of the antagonistic actinomycete. After the biocontrol agent of the antagonistic actinomycete is applied to continuously cropped soil, the biological control rate reaches greater than 70 percent, and has great significance on preventing and controlling the soil-borne diseases of strawberry, improving the quality of agricultural products and protecting the environment.

The invention belongs to the field of the microorganisms and particularly relates to burkholderia LH-ZY01 (burkholderiasp.), and also relates to a strain and application thereof in suppressing rhizoctonia solani. The strain, namely the burkholderia LH-ZY01 (burkholderiasp.), is preserved in CCTCC (China Center For Type Culture Collection) on December 18, 2012; the preservation number of strain is CCTCCNO: M2012536. The burkholderia LH-ZY01 is combined with a loose nutrition carrier and the fermentation technology and the drying technology are adopted, so that the burkholderia is capable of growing in the nutrition carrier, and good in late-time stability and long in retention period. If the strain provided by the invention is applied to soil of crops infected by rhizoctonia solani such as rice, cotton, rape and tomato, diseases caused by the pathogenic bacteria can be remarkably eliminated; the efficiency of the strain for rice sheath blight disease is more than 75% and the efficiency for damping-off of colza is more than 59%; the diseases can be fundamentally eliminated from the soil to which the fertilizer is applied continuously for three crops.