Bacterial strain WXX-2 and bacterial agent for controlling peanut root rot

A WXX-2, peanut root technology, applied in the direction of chemicals for biological control, methods based on microorganisms, biocides, etc., can solve problems such as unstable effects, achieve low cost, less amount of bacterial agents, and control Effects of Peanut Root Rot Disease

Active Publication Date: 2016-11-16
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, general biocontrol agents only control the occurrence of soil-borne diseases from the perspective of plant growth promotion and microbial competition, and the effect is often unstable.

Method used

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  • Bacterial strain WXX-2 and bacterial agent for controlling peanut root rot
  • Bacterial strain WXX-2 and bacterial agent for controlling peanut root rot
  • Bacterial strain WXX-2 and bacterial agent for controlling peanut root rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The peanut rhizosphere soil of a piece of continuous cropping peanut field in the red soil area was collected for related experiments, and the specific steps were as follows:

[0030] (1) Soil sample treatment: prepare sterilized enrichment medium (ingredients: 2.5g of fine powder chitin, MgSO 4 ·7H 2 O 0.5g, K 2 HPO 4 0.7g, KH 2 PO 4 0.3g, FeSO 4 ·7H 2 (0.01g, distilled water 1000ml), sub-pack 20ml in a sterile Erlenmeyer flask, and get 1.0g of fresh soil sample into the Erlenmeyer flask, mix well, and cultivate at room temperature for 48h at 150r / min to obtain an enriched culture solution.

[0031] (2) Separation by coating: absorb 1mL filtrate and add 9.0mL sterile water, serially dilute 3 times to obtain a dilution of 10 -4 Gradiently dilute the sample solution, spread 0.02mL of the soil dilution on the plate separation medium, incubate at 28°C for 4-7d, observe and pick the bacterial strains that produce transparent circles.

[0032] (3) Cultivation and pur...

Embodiment 2

[0038] Embodiment 2: Antibacterial experiment to various root rot pathogens

[0039] Utilize preservation number to carry out antibacterial experiment with the bacterial strain that is CGMCC NO.11540: first described bacterial strain is activated, inoculates pathogenic fungus earlier in the center of PDA plate, punches at the edge of pathogenic bacteria colony with the hole puncher of diameter 5mm to make bacteria cake, and inoculates To the center of the PDA plate, and then inoculate the bacterial strain to be tested about 2.5cm away from the pathogenic bacteria around the petri dish. Each strain of bacteria has three replicates, cultured at 28°C for one week, observe whether there is an inhibition zone, and use the cross method to measure the diameter of the pathogenic fungal colony , to calculate the inhibition rate. The selected pathogens are: Rhizoctonia solani (Rhizoctonia solani), Fusarium solani (Fusarium solani) and Fusarium oxysporum f.sp.diath, such as figure 2 — ...

Embodiment 3

[0042] Embodiment 3: Greenhouse living body control experiment

[0043] Activate the peanut root rot pathogen (Fusarium solani) in PDA medium and culture it at 28°C for 7 days, wash the spores of the pathogenic bacteria on the medium with sterile water, filter the bacterial solution with sterilized filter paper to remove the hyphae, and the pathogenic bacteria will Dilute the filtrate with appropriate distilled water to a spore concentration of 1×10 7 Individual / mL suspension, set aside.

[0044] Add the biocontrol strains for testing without fungal hyphae inducer culture medium (beef extract 3%, peptone 8%) to carry out liquid fermentation (26°C, 72h, 200rpm), and then inoculate sterilized mushrooms in a 5% ratio. The powder matrix was placed in an incubator (28°C) for 5 days, and it was used as bacterial agent A for later use.

[0045] Simultaneously according to above-mentioned same method, just add inactivated fungal mycelia inducer (3%) to carry out bacteriostasis and s...

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Abstract

The invention relates to a bacterial strain WXX-2 and a bacterial agent for controlling peanut root rot. The bacterial strain is Bacillus pumilus with the preservation number of CGMCC No. 11540. The 16s rRNA base sequence of the bacterial strain is shown as SEQ ID No.1. Chitinase produced by the bacterial strain and used for degrading fungal hypha cell walls has higher activity ad has significant degradation capacity against fungal pathogen hyphae such as fusarium oxysporum, fusarium solani, blight bacteria, rhizoctonia and the like infecting roots of crops. The quantity of seed dressing with the bacterial agent is generally only 10-15 kg / mu, damage by the frequent peanut root rot in upland red soil can be effectively controlled, the control effect reaches 60% or higher, and the bacterial strain WXX-2 and the bacterial agent have the advantages of small application quantity, broad antibacterial spectrum, remarkable yield increasing effect and the like, and the influence of the root rot on the loss of peanut yield is effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of agricultural intensive production, and in particular relates to bacterial strains and bacterial agents for preventing and treating peanut root rot diseases. Background technique [0002] Peanut is an important oil crop in my country, and the annual sown area accounts for about 35% of the total sown area of ​​oil crops. The southern red soil hilly area is one of the main peanut producing areas in my country, but continuous cropping is common. Continuous cropping of peanuts often leads to aggravation of soil-borne fungal diseases, among which root rot is the most serious disease in continuous peanut cropping in red soil areas, and is an important factor restricting local peanut production. [0003] The current commonly used treatment method is the application of chemical fungicides. Chemical bacteria agents or high-efficiency fumigants can effectively kill the base of soil pathogenic bacteria and temporar...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/205C12R2001/07
Inventor 李孝刚王兴祥李忠佩丁昌峰周志高
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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