225 results about "Fusarium solani" patented technology

The invention discloses a bacillus belleus GT11 and application thereof. The bacillus belleus CT11 is collected at China General Microbiological Culture Collection Center on December 13th, 2016; the collection number is CGMCC No.13446; the bacillus belleus CT11 can be prepared into water agent, wettable powder or coating material and can be used for preventing and controlling the root rot diseases of pseudo-ginseng and plukenetia volubilis and Chinese cabbage clubroot. The invention provides an efficient, nontoxic, safe, non-residual and conveniently used biological agent for preventing and controlling the diseases such as root rot and clubroot; the effective active ingredient is bacillus belleus CT11 strain; the strain can generate the secondary metabolite antimicrobial active matter, is capable of degrading the cell wall of pathogenic bacteria and has an excellent growth-promoting effect for the crops, such as, plukenetia volubilis, pseudo-ginseng and Chinese cabbage; besides, the bacillus belleus CT11 has a better antibacterial effect for pseudo-ginseng phytophthora sojae, pseudo-ginseng fusarium solani, plukenetia volubilis gray mold, paddy bacterial mycosphaerella, and the like.

A method of determining the presence of soybean sudden death syndrome resistance in the soybean plant in a greenhouse setting, the method comprising the steps of: (a) inoculating soil with a low density inoculum of Fusarium solani; (b) planting a soybean plant in said inoculated soil; (c) growing said plant in said soil in a greenhouse; (d) isolating Fusarium solani-infected tissue from said plant; (e) culturing said infected tissue for a period of time sufficient to allow for fungal colony forming unit growth; (f) scoring at least one of disease severity and infection severity in said plant using the number of said fungal colony forming units; and (g) correlating at least one of said disease severity and said infection severity to at least one of disease severity and infection severity data from genetic markers associated with soybean sudden death syndrome resistance to identify a correlation, wherein a statistically significant correlation indicates presence of soybean sudden death syndrome resistance in said soybean plant. Also provided is a method of characterizing resistance to soybean sudden death syndrome in a soybean plant, the method comprising the steps of: (a) isolating roots from a soybean plant infected by Fusariurn solani; (b) culturing the root on a culture plate including a restrictive growth medium that provides for slow fungal growth and restricted bacterial growth; (c) determining root infection severity by statistically evaluating the number of Fusarium solani colony forming units on said culture plate; and (d) characterizing resistance to soybean sudden death syndrome in said soybean plant based on said determined root infection severity.

The invention provides bacillus methylotrophicus with a collection number of CCTCC M2015297. Bacillus methylotrophicus is named bacillus methylotrophicus XK-4. The invention further provides a screening method of bacillus methylotrophicus XK-4, an application of bacillus methylotrophicus XK-4 to inhibition of sclerotinia sclerotiorum, rhizoctonia solani, fusarium solani and fusarium oxysporum and a bacillus methylotrophicus XK-4 agent. Bacillus methylotrophicus XK-4 is a rape rhizosphere microorganism, is safe to human, livestock and crops, is environment-friendly, has a wider antibacterial spectrum, has better inhibitory effects on mycelial growth of common pathogenic bacteria, such as sclerotinia sclerotiorum, rhizoctonia solani, fusarium solani and fusarium oxysporum, has obvious inhibitory effects on mycelial growth of sclerotinia sclerotiorum and causes mycelia deformation. The further prepared agent has obvious control effects on sclerotinia rot of colza, is short in production cycle and has a very extensive application prospect in biological control of plant diseases.

The invention relates to methods and compositions for identifying soybean plants that are tolerant, have improved tolerance or are susceptible to Fusarium solani infection (the causative agent of sudden death syndrome or SDS). The methods use molecular genetic markers to identify, select and / or construct disease-tolerant plants or identify and counterselect disease-susceptible plants. Soybean plants that display tolerance or improved tolerance to Fusarium solani infection that are generated by the methods of the invention are also a feature of the invention.

The invention discloses a panax notoginseng antimicrobial peptide gene PnSN1. The nucleotide sequence of the PnSN1 gene is shown as SEQ ID NO:1, and the PnSN1 gene is used for coding snakin antimicrobial peptide. The functional genomics correlation technique study proves that the PnSN1 gene has the function of improving the plant fungal infection resistance capability; the antifungal PnSN1 gene is built onto a plant expression vector and is transferred into tobaccos for overexpression; the transgenosis tobacco has high in vitro antifungal activity. The transgenosis tobacco subjected to PnSN1 overexpression has the strong inhibiting effect on the growth of fusarium oxysporum, fusarium solani, verticillium fusarium, botryosphaeria dothidea and beaded gibberella.

The invention relates to a bacterial strain for preventing potato blight and a preparation method and the application of a preparation thereof. The bacterial strain is bacillus subtilis MES810, the preservation date is Aug, 10th, 2017, and the preservation number is CGMCCNo. 145154. The preparation method of the preparation corresponding to the bacterial strain comprises the following steps: activation of a culture; preparation of a primary seed solution; preparation of a secondary seed solution; fermentation in a fermentation tank, and discharging to obtain a bacillus subtilis liquid preparation. The bacillus subtilis has a strong inhibiting effect for fusarium pathogenic bacteria consisting of Fusarium solani, fusarium oxysporum, fusarium moniliforme, Fusarium nivale or elderberry fusarium and Eavenaceum, the occurrence of potato blight is prevented, the control efficiency is more than 80%, and the increase of both production and income for potato is promoted. The bacterial strain disclosed by the invention has the characteristics of no toxicity, no residue, no growth inhibition, small using amount and simple application method, and provides the possibility for the diversification of application schemes and the protection of ecological environment.

A process of preparing chitosan used Fusarium Solami. Chitosan produced from Fusarium Solami is a kind of high efficient inscribed enzyme. Viscosity of chitosan solution can reduce to 90% in 2h between 37deg.C and 55deg.C. Producing field is bigger than 70% after 8h. Content of oligosaccharide below 10 is more than 90%. The process contains strain culture, ferment process, pre-process of material, filtering of enzymolysised solution, molecular weight assortment, decolour, desalting, concentrating and drying.

The invention relates to methods and compositions for identifying soybean plants that are tolerant, have improved tolerance or are susceptible to Fusarium solani infection (the causative agent of sudden death syndrome or SDS). The methods use molecular genetic markers to identify, select and / or construct disease-tolerant plants or identify and counterselect disease-susceptible plants. Soybean plants that display tolerance or improved tolerance to Fusaruim solani infection that are generated by the methods of the invention are also a feature of the invention.

The invention discloses a lilium regale inducible promoter PD1 and application thereof, the nucleotide sequence of the inducible promoter PD1 is shown as SEQ ID NO:1, and the lilium regale inducible promoter PD1 is proved to respond to several plant hormones, biological stress and abiotic stress through molecular biology and genetic engineering related technical research; a fusion gene expression cassette constructed by the lilium regale promoter PD1 and a [beta]-glucuronidase gene is transferred into tobacco for expression, and the glucuronidase activity of transgenic tobacco is quantitatively detected through adoption of a fluorescence method. Results show that after the transgenic tobacco is treated by abscisic acid, salicylic acid, methyl jasmonate, gibberellin, ethephon, fusarium solani, injury stress and sodium chloride, the activity of glucosidase is obviously enhanced; and the inducible promoter PD1 disclosed by the invention has a wide application prospect in genetic engineering for resisting biological or abiotic stress.

The invention relates to solid fermentation of trichoderma harzianum. The solid fermentation of the trichoderma harzianum comprises the steps that trichoderma harzianum M-17 strains are inoculated into a solid culture medium for culture at 28 DEG C for 7-10 days, and according to the formula, the culture medium is prepared from the components of Chinese medicinal powder, potato flakes, corn flour,wood chips and potato skins. The trichoderma harzianum cultured by the method has a good inhibiting effect on pathogenic bacteria of potato root rots such as fusarium equiseti, fusarium sambucinum, fusarium oxysporum, fusarium solani and fusarium acuminatum, and can be applied to the prevention and control process of the potato root rots, the occurrence of the potato root rots is reduced and prevented, the yield of potatoes is increased and the quality of the potatoes is improved.