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Bacillus methylotrophicus and application thereof

A methylotrophic, bacillus technology, applied in the application, bacteria, fungicides and other directions, to achieve the effects of simple use, short production cycle and low cost

Inactive Publication Date: 2015-11-04
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports of methylotrophic Bacillus that have an inhibitory effect on Sclerotinia sclerotiorum

Method used

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  • Bacillus methylotrophicus and application thereof
  • Bacillus methylotrophicus and application thereof
  • Bacillus methylotrophicus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Rapeseed Sclerotinia, rice sheath blight, watermelon Fusarium wilt, and walnut root rot described in this embodiment are provided by the Plant Pathology Research Office of the Agricultural College of Changjiang University. Rapeseed Sclerotinia and rice sheath blight obtained in other ways , Fusarium wilt of watermelon, root rot of walnut are also applicable to the present invention.

[0042] 1. Isolation, screening and identification of methylotrophic Bacillus XK-4

[0043] Isolation: Vigorously growing rapeseed plants were collected from the rapeseed fields around Jingzhou City, Hubei Province, and the rhizosphere soil adhered to the roots was taken and placed in a 250mL triangular flask. Pack the Sclerotinia sclerotiorum sclerotia collected in the previous growing season into 5cm 2 Size, mesh is in the nylon pouch of 1.5mm2, every bag 10, then nylon bag is buried in the above-mentioned triangular bottle that fills rhizosphere soil. Three weeks later, take out the sc...

Embodiment 2

[0095] Embodiment 2 is basically the same as Embodiment 1, the difference is:

[0096] This embodiment completes the preparation of the methylotrophic bacillus XK-4 inoculum according to the following main steps:

[0097] (1) Inoculate the methylotrophic Bacillus XK-4 on LB solid medium, cultivate at 30°C for 22h, and activate the strain; LB solid medium preparation: 5g of yeast extract, 10g of tryptone, chlorine Sodium chloride 10g, agar powder 20g, NaOH to adjust the pH to 7.1, 121 ℃, 20min autoclave.

[0098] (2) Insert the strain activated in step (1) into LB liquid medium, shake and culture at 155r / min at 30°C for 22h to make seed liquid; LB liquid medium preparation: 5g of yeast extract, 10g of tryptone , sodium chloride 10g, NaOH to adjust the pH to 7.1, 121 ℃, 20min autoclaving.

[0099] (3) Inoculate the seed fermentation broth obtained in step (2) at 5% by volume in LB liquid fermentation medium, and shake and cultivate at 30° C. at 140 r / min for 42 hours.

[0100...

Embodiment 3

[0102] Embodiment 3 is basically the same as Embodiment 1, the difference is:

[0103] This embodiment completes the preparation of the methylotrophic bacillus XK-4 inoculum according to the following main steps:

[0104] (1) Inoculate the methylotrophic Bacillus XK-4 on LB solid medium, cultivate at 30°C for 24h, and activate the strain; LB solid medium preparation: 5g of yeast extract, 10g of tryptone, chlorine Sodium chloride 10g, agar powder 20g, NaOH to adjust the pH to 7.2, 121 ℃, 20min autoclaving.

[0105] (2) Insert the activated bacterial strain in step (1) into LB liquid medium, shake and culture at 165r / min at 30°C for 24h to make seed liquid; LB liquid medium preparation: 5g of yeast extract, 10g of tryptone , sodium chloride 10g, NaOH to adjust the pH to 7.2, 121 ℃, 20min autoclaving.

[0106] (3) Inoculate the seed fermentation broth obtained in step (2) at 5% by volume in LB liquid fermentation medium, and shake and cultivate at 30° C. at 140 r / min for 44 hou...

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Abstract

The invention provides bacillus methylotrophicus with a collection number of CCTCC M2015297. Bacillus methylotrophicus is named bacillus methylotrophicus XK-4. The invention further provides a screening method of bacillus methylotrophicus XK-4, an application of bacillus methylotrophicus XK-4 to inhibition of sclerotinia sclerotiorum, rhizoctonia solani, fusarium solani and fusarium oxysporum and a bacillus methylotrophicus XK-4 agent. Bacillus methylotrophicus XK-4 is a rape rhizosphere microorganism, is safe to human, livestock and crops, is environment-friendly, has a wider antibacterial spectrum, has better inhibitory effects on mycelial growth of common pathogenic bacteria, such as sclerotinia sclerotiorum, rhizoctonia solani, fusarium solani and fusarium oxysporum, has obvious inhibitory effects on mycelial growth of sclerotinia sclerotiorum and causes mycelia deformation. The further prepared agent has obvious control effects on sclerotinia rot of colza, is short in production cycle and has a very extensive application prospect in biological control of plant diseases.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to a methylotrophic bacillus and an application thereof. Background technique [0002] Rapeseed is one of the important oil crops in my country, and its planting area and output both rank first in the world. Sclerotinia sclerotiorum caused by Sclerotinia sclerotiorum infection is an important disease, ranking first among the three major diseases of rapeseed in my country. It occurs and is prevalent in nearly one hundred countries on five continents. In recent years, with the popularization of no-tillage direct seeding cultivation technology of rapeseed, the incidence of Sclerotinia sclerotiorum has been increasing year by year, and the annual average incidence rate in the Yangtze River Basin has risen to 15-30%. The occurrence of rapeseed sclerotinia will seriously affect the yield and quality of rapeseed, and cause significant economic loss...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
Inventor 孙正祥张长青马东方周京龙
Owner YANGTZE UNIVERSITY
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