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Bacillus subtilis with antibacterial activity and application of bacillus subtilis

A Bacillus subtilis, antibacterial activity technology, applied in the field of Bacillus subtilis, can solve the problems of increasing the difficulty and cost of treatment, drug resistance, drug resistance, poor BV effect, etc., to avoid recurrent attacks, antibacterial Broad spectrum, speed up the effect of balance

Active Publication Date: 2015-03-25
山东宝来利来生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antibiotics kill pathogenic microorganisms and normal flora at the same time, causing excessive reproduction of pathogenic bacteria or conditional pathogenic bacteria, resulting in repeated infections and multiple infections, resulting in drug resistance and resistance, increasing the difficulty and cost of treatment
Traditional Chinese medicine treatment, using the method of fumigation and washing, often has poor effect on BV. At present, the common Chinese patent medicine gels, suppositories, effervescent tablets, etc. on the market are mainly used for adjuvant treatment of antibacterial drugs
According to the survey, many drugs are not effective, especially for VVC and recurrent vulvovaginal candidiasis (RVVC), the course of treatment is long and easy to repeat

Method used

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  • Bacillus subtilis with antibacterial activity and application of bacillus subtilis
  • Bacillus subtilis with antibacterial activity and application of bacillus subtilis
  • Bacillus subtilis with antibacterial activity and application of bacillus subtilis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1: the acquisition and identification of bacterial strain JYM841

[0053] 1. Obtaining of strain JYM841

[0054] 1. Medium

[0055] (1) Tryptone soybean agar medium: tryptone 1.5%, soybean peptone 0.5%, sodium chloride 0.5%, agar 1.6%, pH7.2;

[0056] (2) Sandcastle agar medium: 4% glucose, 1% peptone, 2% agar, pH5;

[0057] (3) Nutrient agar medium: beef extract 0.5%, peptone 1%, sodium chloride 0.5%, pH 7.2-7.4, agar 1.6%.

[0058] (4) Bacillus basal medium: glucose 0.2%, peptone 1.0%, sodium chloride 0.5%, yeast extract 0.5%, pH 7.0.

[0059] (5) Lactic acid bacteria medium (MRS medium): glucose 2.0%, ammonium citrate 0.2%, sodium acetate 0.5%, dipotassium hydrogen phosphate 0.5%, manganese sulfate 0.02%, magnesium sulfate 0.05%, peptone 1.0%, beef extract 1.0%, yeast extract 0.5%, Tween-800.1%, pH6.0.

[0060] (6) LB liquid medium: glucose 0.2%, peptone 1.0%, sodium chloride 0.5%, yeast extract 0.5%, pH 7.0.

[0061] (7) LB slant medium: glucose 0.2...

Embodiment 2

[0079] Example 2: Research on the characteristics of metabolites of bacterial strain JYM841

[0080] 1. Test material:

[0081] 1.1 Tested strains: Bacillus subtilis N9-1-35 (preserved in China Center for Type Culture Collection on August 31, 2011, and its preservation number is: CCTCC M 2011301); Bacillus subtilis B7348 (preserved in 2010 It was preserved in the China Center for Type Culture Collection on October 12, and its preservation number is CCTCC M 2010260); the strains JYM811, JYM812, JYM831, JYM841, JYM851, JYM862 and JYMX obtained by screening in Example 1.

[0082] 1.2 Indicator bacteria: Candida albicans (marked as BN), Staphylococcus aureus (marked as JP).

[0083] 2. Test method and result:

[0084] (1) Temperature tolerance of extracellular antimicrobial proteins

[0085] The preparation of the metabolites of the bacterial strains was the same as in Example 1. The metabolites of the bacterial strains JYM812, JYM831, JYM841, JYM851, and JYM862 were treated at 6...

Embodiment 3

[0128] Embodiment 3: the antibacterial spectrum research of bacterial strain JYM841

[0129] 1. Test material:

[0130] 1.1 Tested strains: strain JYM841 screened in Example 1.

[0131] 1.2 Indicator bacteria: Candida albicans, Staphylococcus aureus, Escherichia coli, Lactobacillus plantarum LP, Streptococcus thermophilus 1.2471, Lactobacillus acidophilus 1.2467, Lactobacillus casei 1.0062, Lactobacillus helveticus 1.1877. Among them, Lactobacillus plantarum LP (preserved in China Center for Type Culture Collection on June 21, 2010, its preservation number is: CCTCC M 2010150, recorded in Chinese patent application CN201010240725.X); the rest of the indicator bacteria are commercially available product.

[0132] 2. Methods and results:

[0133] Get the fermentation broth of bacterial strain JYM841 and centrifuge at 10000rpm for 10min, take the supernatant, and carry out the antibacterial test; Lactobacillus acid 1.2467, Lactobacillus casei 1.0062, Lactobacillus helveticus ...

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Abstract

The invention discloses bacillus subtilis JYM841 with antibacterial activity. The bacillus subtilis is preserved in a China center for type culture collection in January 10, 2014, and the preservation number is CCTCC NO: M 2014012. The bacillus subtilis JYM841 has a strong inhibitory effect on staphylococcus aureus and candida albicans, is high in antibacterial activity, wide in antibacterial spectrum, high in enzyme production, sensitive to bacterial antibiotic, drug-resistant to fungal antibiotic, high in heat-resisting ability and tolerant to acid and alkaline, has high antibacterial activity after treated at the temperature of 100 DEG C for 20min, and has tolerance to various enzymes such as trypsin, papain and protease K. An acid and alkali resistant pH (potential of hydrogen) value ranges from 6 to 10, and metabolite is stable, so that the bacillus subtilis can be applied to preparation of medicines for treating genital tract infection.

Description

technical field [0001] The invention relates to a strain of Bacillus subtilis with antibacterial activity and application thereof. Background technique [0002] Antibiotics are some microorganisms that convert primary metabolites into structural secondary metabolites through enzymatic reactions. The unreasonable large-scale use and abuse of antibiotics, coupled with the lack of strict supervision, lead to death, the emergence of drug-resistant microorganisms and super-resistant strains, and the misuse of antibiotics also puts patients at unnecessary risk of drug resistance; adverse drug reactions The cost of hospitalization caused by antibiotics has grown to hundreds of millions of dollars, greatly increasing medical costs, and the side effects of antibiotic abuse have become a global problem that needs to be solved urgently. [0003] Vulvovaginal candidiasis (VVC) and bacterial vaginosis (Bacterial Vaginosis, BV) are an infection caused by vaginal microecological imbalance...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/742A61P31/04A61P31/10A61P15/00C12R1/125
CPCA61K35/742C12N1/20C12N1/205C12R2001/125Y02A50/30
Inventor 单宝龙陈雷谷巍程秀芳王红刘虹张建梅黄启亮翟延庆
Owner 山东宝来利来生物工程股份有限公司
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