119results about How to "High enzyme production" patented technology

The invention relates to crop stalk fermentation and lactic acid and quality fodder preparation method. The synchronous diastatic fermentation provided by the invention is: adding in pretreated stalk of 2-3 times water in weight, 121 DEG C sterilizing, adding cellulase leaven (step 2 prepared), enzyme activity of cellulase leaven of total substrate amounting to 42-50IU / g in cellulose filter paper enzyme activity, inoculating activated high temperature resistant lactobacillus, inoculating volume is 5-10% of total substrate, fermenting 72-108 hours in 45-50 DEG C condition, adjusting substrate pH to 5.0-6.0 during fermenting. The invention shortens greatly of fermentation time, promotes the fermentation effiency. The 72-108 hours synchronous fermentation can produce 6-8g lactic acid and 60-70g quality fodder every 100g dry stalk. The invention has merits of low cost, low investment, no pollution, little energy consumption, high resource exploitation.

The present invention provides one kind of Aspergillus niger WZ001 capable of realizing industrial production of producing naringinase and cirmtimase simultaneously in high yield and its application. The Aspergillus niger WZ001 has the preservation number of CCTCC No.206047. It has high yield of naringinase and cirmtimase over 14000 U / g in liquid fermentation culture or over 6000 U / g in solid fermentation culture.

The invention relates to a microorganism multienzyme additive for promoting the growth and development of baby pigs, which is characterized in that the additive is formed by composite synchronizing solid fermentation of a composite strain stock solution, carbon source, nitrogen source, inorganic salt and enzyme revulsant. A manufacturing method is that all the strains adopt a composite inducing technology to carry through filtering; a method of synchronizing liquid culture and solid composite culture is adopted to lead all the strains to be cultivated in the same culture medium; the total number of live bacterium reaches 3 billion / g and simultaneously a fermented product is kept completely. The product comprises massive physiological metabolism substances like active enzyme, small peptide, oxidation resistance substances, FAA, vitamins, etc. A specific type for feed preparing particles carries through a double-layer microcapsule peridium treatment on the strains that can not resist heat. The invention has the advantages of promoting the digestive absorption on the feed by the baby pigs, promoting the compound of organism protein and full utilization, increasing the disease resistance and immunological competence of the baby pigs, improving the feed switching rate and the production capacity of cultivated animals, reducing the pollution to the environment caused by animal dejecta. The invention is a green and safe agrotechnical product.

The invention discloses Bacillus subtilis high in nattokinase secretion and application thereof. The strain is named as Bacillus subtilis gs-11061 and is preserved in China General Microbiological Culture Collection Center, and the collection number of the Bacillus subtilis gs-11061 is CGMCC No. 13932. The invention further discloses a method using the Bacillus subtilis high in nattokinase yield to produce nattokinase. Compared with other fermentation methods, the method has the advantages that the Bacillus subtilis can be fermented under high temperature, and the fermentation cycle is shortened evidently; in terms of a culture medium, a xylose mother liquor coarse raw material is used to lower cost, and low energy consumption and low pollution are achieved; compared with original bacteria, the secretion of the nattokinase is increased evidently, fermentation products can be processed easily, and few byproducts are produced.

The invention relates to a high-yield 'cellulose, hemicelluloses and lignin enzyme' straw decomposition additive and a preparation method thereof. The high-yield 'cellulose, hemicelluloses and ligninenzyme' straw decomposition additive comprises the following preparation raw materials: a cellulose, hemicelluloses and lignin enzyme mixture, plant materials, sugar and urea, wherein the cellulose, hemicelluloses and lignin enzyme mixture consists of cellulase bacillus subtilis fermentation liquor, a rhizopus oryzae fermentation substance and ligninase white-rot fungi fermentation liquor; the weight ratio of the cellulase bacillus subtilis fermentation liquor to the rhizopus oryzae fermentation substance to the ligninase white-rot fungi fermentation liquor is 3-7 to 5-10 to 5-10. By adoptingthe high-yield 'cellulose, hemicelluloses and lignin enzyme' straw decomposition additive provided by the invention, different bacterial florae can harmoniously coexist and be mutually promoted, an effect of complementary advantages can be achieved, the effective viable count can be increased, the enzyme yield can be increased, cellulose, hemicelluloses and lignin in crop straw can be rapidly andeffectively decomposed, and rapid treatment and circulation of straw biomass resources can be achieved.

The invention relates to a associated fermentation technique of germzymin acted as fodder additive. Its process flow is as follows: to plant multiple bacterial spawn of enzyme production and multipleready produced bacterial spawn on the slope respectively, after ripeness to be developed into spore suspension; then mixing said of each kind respectively according to proportion; hence seed culture medium to be led into enzyme production and spore suspension of ready produced and mixed bacteria spawn; finally through developing seed culture medium sets up full spore, mixing seed disinfectant of enzyme production germ and of ready produced bacterium, developing into mixture transfer agent of germ and enzyme, thereafter ferment breeding, products after ferment to be treated and become germzymin.

The invention relates the vibrionaceae vibrio strain and its application. The invention relates the vibrionaceae vibrio strain from sea-tangle, and the output of algin lyase made by the vibrionaceae vibrio strain is 2-5 times than that of algin lyase made with now technology. The algin lyase can be used to make algin oligosaccharide.

The invention relates to a detoxifying agent for biologically degrading ochratoxin A in feeds and a preparation technology of the detoxifying agent. The detoxifying agent comprises 40-60 parts of bentonite, 20 parts of immune polysaccharides, 10-20 parts of a free-radical scavenger and 20-30 parts of bacillus subtilis powder for degrading the ochratoxin A, wherein the bacillus subtilis powder is prepared through the following steps of (1) performing seed liquor activation on original strains, and performing mother seed culturing in a first-class fermentation tank loaded with culture mediums; (2) performing amplified culturing on mother seed culturing liquor in a second-class fermentation tank loaded with LB culture mediums; and (3) separating thalli from fermentation liquor, performing concentration, and performing drying so as to prepare the bacillus subtilis powder for biologically degrading the ochratoxin A. The bacillus subtilis powder, a first-time mixture and a second-time mixture in parts by weight are compounded so that a finished product of the detoxifying agent is obtained. According to the detoxifying agent disclosed by the invention, the degradation rate of the detoxifying agent for the ochratoxin A is as high as 92% or above, the degradation efficiency is high, and defects of an adsorbent are overcome; and besides, immune polysaccharide and free-radical removing ingredients are added to products, so that the functions of protecting the liver and enhancing the immunity of organisms can be achieved, and the effects are obvious.

The invention provides a pectinase producing bacterial strain, namely Asperillus niger WZW001, and an application in preparation of pectinase and peeled citrous complex enzyme. The strain is preserved in the CCTCC (china center for type culture collection) on Oct. 12, 2012, and the preservation No. is CCTCC No: M2012399. The peeled citrous complex enzyme prepared by the Asperillus niger WZW001 has the advantages of low production cost, specificity and efficiency, stable application and the like; during the application, as long as the Asperillus niger WZW001 strain fermentation liquor is used for preparing solid pectinase or is separated and purified to obtain PL, Exo-PG, and PE single enzyme elements, and one or more of the solid pectinase or the single enzyme elements is compounded with marketed cellulose without adding other enzymes, so that the orange capsule can be efficiently removed. According to the pectinase producing bacterial strain and the application, the problem of high cost of enzyme for an enzyme-method capsule removing process at present is solved, the replacement of the process to an acid-alkali chemical method is facilitated, and the energy-saving, emission-reduction, green and efficient production of the orange deep processing industry in the country has practical significance.

The invention discloses chitosanase OUC-CsnCA. The amino acid sequence of the chitosanase OUC-CsnCA is as shown in SEQ ID NO. 1. The invention also discloses a gene for encoding the chitosanase OUC-CsnCA, and the nucleotide sequence of the gene is shown as SEQ ID NO.2. The invention also discloses a preparation method of the chitosanase OUC-CsnCA. The invention also discloses application of the chitosanase OUC-CsnCA in degradation of chitosan / preparation of chitosan oligosaccharide, and further relates to application of the chitosanase OUC-CsnCA in preparation of an enzyme preparation for degrading chitosan. The chitosanase OUC-CsnCA disclosed by the invention is used for degrading chitosan to generate chitosan oligosaccharide, the optimum pH value of the chitosanase OUC-CsnCA is 8.0, theoptimum reaction temperature of the chitosanase OUC-CsnCA is 55 DEG C, and the specific enzyme activity of the chitosanase OUC-CsnCA is 1786.227 U / mg; in addition the enzyme activity and the enzyme production level are high, wherein the protein concentration after purification is 1.284 mg / ml, and compared with reported chitosanase, the chitosanase has certain advantages. The chitosanase OUC-CsnCAdisclosed by the invention has important industrial application value and economic value.