Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing phosphoglyceric oxidase using inducing culture substrate chain coccus

A technology of phosphoglycerol oxidase and inducing medium, which is applied in the field of producing phosphoglycerol oxidase by using inducing medium

Inactive Publication Date: 2007-11-14
王腾 +1
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 1997, Feng Yongmei, Institute of Microbiology, Chinese Academy of Sciences, etc. reported the research on the strain selection and fermentation conditions of phosphoglycerol oxidase, and there have been no related research reports since then.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] One, make culture medium (weight percentage)

[0018] Slant induction medium (%): yeast extract 2.5, peptone 2.0, glucose 0.5, dipotassium hydrogen phosphate 0.8, glycerol 0.5, agar 3.0, magnesium sulfate 4, manganese chloride 1, sodium chloride 20, pH 6.8 0.4 ml of saline solution;

[0019] Seed medium (%): yeast extract 2.5, peptone 2.0, glucose 0.8, dipotassium hydrogen phosphate 0.8, glycerol 0.5, magnesium sulfate 4, manganese chloride 1, sodium chloride 20, pH 6.8 mixed salt solution 0.4 ml ;

[0020] Fermentation medium (%): yeast extract 2.5, peptone 2.0, dipotassium hydrogen phosphate 2.0, glycerol 0.5, magnesium sulfate 4, manganese chloride 1, sodium chloride 20, 0.4 ml of mixed salt solution of pH 6.8;

[0021] 2. Cultivate strains

[0022] After the strain was cultured on a slant medium at 32°C for 28 hours, it was inserted into the seed medium, and cultured with shaking at 32°C and 200 rpm for 24 hours. The seed solution was added to the fermentation m...

Embodiment 2

[0030] 1. Production medium (%):

[0031] Slant induction medium: yeast extract 0.5, peptone 1.0, glucose 0.05, dipotassium hydrogen phosphate 0.2, glycerin 0.1, agar 1.5, magnesium sulfate 4, manganese chloride 1, sodium chloride 20, pH 6.8

[0032] Mixed salt solution 0.1 ml;

[0033] Seed medium: yeast extract 0.5, peptone 1.0, glucose 0.1, dipotassium hydrogen phosphate 0.2, glycerin 0.1, magnesium sulfate 4, manganese chloride 1, sodium chloride 20, 0.1 ml of mixed salt solution with pH 6.8;

[0034] Fermentation medium: 0.5 yeast extract, 1.0 peptone, 0.5 dipotassium hydrogen phosphate, 0.1 glycerol, 4 magnesium sulfate, 1 manganese chloride, 20 sodium chloride, 0.1 ml of mixed salt solution with pH 6.8;

[0035] 2. Cultivate strains

[0036] After the strain was cultured on the slant medium at 25°C for 18 hours, it was inserted into the seed medium, and cultured with shaking at 25°C and 200 rpm for 18 hours. The seed solution was added to the fermentation medium at a...

Embodiment 3

[0039] 1. Production medium (%)

[0040] Slant induction medium: yeast extract 1.0, peptone 1.5, glucose 0.25, dipotassium hydrogen phosphate 0.5, glycerin 0.12, agar 2.0, magnesium sulfate 4, manganese chloride 1, sodium chloride 20, pH 6.8 mixed salt solution 0.2 ml;

[0041] Seed medium: 0.5 yeast extract, 1.0 peptone, 0.5 glucose, 0.5 dipotassium hydrogen phosphate, 0.1 glycerol, 4 magnesium sulfate, 1 manganese chloride, 20 sodium chloride, 0.2 ml of mixed salt solution with pH 6.8;

[0042] Fermentation medium: yeast extract 0.5, peptone 1.0, dipotassium hydrogen phosphate 2.0, glycerin 0.1, magnesium sulfate 4, manganese chloride 1, sodium chloride 20, 0.2 ml of mixed salt solution with pH 6.8;

[0043] 2. Cultivate strains

[0044] After the strain was cultured on the slant medium at 30°C for 22 hours, it was inserted into the seed medium, and cultured at 30°C at 200 rpm for 20 hours. The seed solution was added to the fermentation medium at a ratio of 10%, and cultur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

This invention is with the guide hammer bacterium of culture medium preparation Lin the method of sour glycerine oxidize enzyme. The group of culture medium divides and cultivates condition: Bevel guides culture medium, draw thing by yeast , egg white Dong , glucose , Lin the mixture salt solution of 2 sour hydrogen potassiums, glycerines, agars, the magnesiums of sulphuric acid, chlorination manganese, common salts and pH6.8s forms; Seed culture medium draws thing and egg white by yeast Dong , glucose , Lin the mixture salt solution of 2 sour hydrogen potassiums, glycerines, the magnesiums of sulphuric acid, chlorination manganeses, common salts and pH6.8s forms; Seed culture medium draws thing and egg white by yeast Dong , glucose , Lin the mixture salt solution of 2 sour hydrogen potassiums, glycerines, the magnesiums of sulphuric acid, chlorination manganeses, common salts and pH6.8s forms; Ferment culture medium draws thing and egg white by yeast Dong , Lin the mixture salt solution of 2 sour hydrogen potassiums, glycerines, the magnesiums of sulphuric acid, chlorination manganeses, common salts and pH6.8s forms. Bacterial strain is cultivated by bevel, seed is cultivated , revolving shaking table ferments to train , enlarging again collect bacterium body after training , adoption assembles glycol - the two water of ammonium sulphate appearance extraction and the layer of two steps Xi purification gets Lin sour glycerine oxidize enzyme.

Description

technical field [0001] The invention relates to a method for producing oxidase, in particular to a method for producing phosphoglycerol oxidase with an induction medium. Background technique [0002] The content of triglycerides in serum has been recognized as an important index in the clinical diagnosis of hyperlipidemia and heart disease. The glycerol phosphate oxidase method for the determination of triglyceride content has the advantages of high specificity, small amount, simplicity, rapidity, and automatic instrument determination. It was once recommended by the Chinese Medical Association as a routine method for the determination of serum triglycerides in clinical laboratories. [1，2] . Glycerol phosphate oxidase (L-a-Glycerophosphate Oxidase, GPO, EC 1.1.3.21), also known as glycerol phosphate oxidase, is the last enzyme in the determination reaction of the triglyceride kit, which requires higher specificity and enzyme activity and specific activity. At present, the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12N9/02C12R1/46
Inventor 王腾孟延发
Owner 王腾
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com