848 results about "Active enzyme" patented technology

The invention applies to the tea crop production, which can improve the soil structure and provide adequate moisture, oxygen and nutrition to improve the yield and quality in tea crop cultivation. The invention relates to a liquid fertilizer containing microorganisms, organic matters and inorganic matters. The fertilizer adopts biological technology and electrolysis technology which complement each other, takes micro-organisms and mineralized substances as additive, as well as combines with nutritional formula (nitrogen, phosphorus, potassium, sulfur, magnesium, calcium, silicon, boron, zinc, iron, molybdenum, bacterial protein, amino acid, humus, plant nucleic acid, B vitamins, active enzymes and unknown factors which promote crop growth), so as to provide adequate nutrition for crops and improve soil to make the soil conducive to tea crop cultivation. Through biotechnology, the micro-organisms in a dormant state are added to the fertilizer and are activated under favorable environmental conditions to decompose the animal and plant residues, pesticides, fertilizers, heavy metal residues and minerals in the soil so as to change the contents of nitrogen, phosphorus and potassium. Through electrolysis technology, the mineralized substance additive enables the soil to produce a chain reaction to moisture and oxygen supply, so as to gradually change the ion charge in the soil, increase the soil oxygen supply amount, improve the moisture infiltration capacity and strengthen the conditions for microbial activities.

The present invention is directed to methods and systems for the treatment of inborn genetic errors or other defects that cause deficiencies of active enzymes or proteins within the cells of the central nervous system. Such methods and systems generally comprise an implantable catheter system designed for the chronic delivery of specially formulated proteins to intrathecal, intracerebroventricular, and / or intraparenchymal regions of the central nervous system. The invention has application in the neuropathic aspects of the broad category of lysosomal storage diseases. These genetic based diseases are the result of insufficient enzyme activity to catabolize specific substances, which thereby accumulate in the cellular lysosomes.

Methods for expressing active enzymes are described that involve co-expressing a first enzyme with a second enzyme that has an enzymatic activity that reverses a modification on the first enzyme and / or for identification of soluble and / or active catalytic domains by systematic variation of fragment lengths around catalytic domain boundaries.

The invention relates to storable medicaments produced from pharmaceutical active ingredient preparations which are virus safe. Said medicaments contain at least one intact therapeutic protein obtained from plasma or by means of genetic engineering, as an active pharmaceutical substance. Said active ingredient preparations contain active enzymes, especially proteases, which are either free or bound to the substrates thereof and act against the therapeutic protein(s) present.

The present invention provides an anaerobic digestion process for the treatment of organic waste materials, which process comprises a bacterial process that is carried out in the absence of oxygen and wherein said process comprises digestion, in which said waste is fermented in tanks at an elevated temperature, and wherein said process results in the production of biogas, which can be used in generators for electricity production and / or in boilers for heating purposes, the comprises treating an organic waste with a composition comprising a fermentation supernatant containing active enzymes from a Saccharomyces cerevisiae culture; and a non-ionic surfactant, wherein said nonionic surfactant may be selected from the group consisting of ethoxylated nonylphenol and ethoxylated octyl phenol.

Isolated and / or recombinant enzymes that include surface binding domains, surfaces with active enzymes bound to them and methods of coupling enzymes to surfaces are provided. Enzymes can include large and / or multiple surface coupling domains for surface coupling.

The invention discloses a biogas residue humus pot and a preparation method thereof. The biogas residue humus pot is made of 62.5-186.5 parts of fresh biogas residue, 50-150 parts of clay and 0.5-5 parts of cotton seed hulls or 1-18 parts of wheat straws or corn straws. The fresh biogas residue, the clay and the cotton seed hulls (or wheat / corn straws) are proportionally mixed well to obtain a mixture, the mixture is fed into a mould to be manually or mechanically pressed into an object with a rectangular or disc-shaped seeding hole in the middle, and the object is then stripped and naturally aired for 2-8 days or dried so as to obtain a biogas residue humus pot or seedling raising tray. The biogas residue humus pot is full of nutrients and high in organic content, further contains vitamins B, gibberellin, heteroauxin, amino acid and active enzymes, has fine chemical and biological properties, and meets conditions of compost. The biogas residue humus pot has the advantages of no environmental pollution, natural degradability, no need of stripping, simplicity in preparation, low cost, fastness in seedling raising speed, capability of enhancing seedlings, effectiveness and the like.

Enzyme catalyst immobilized on porous flouropolymer supports. Catalytically active enzymes can be bound to a variety of fluoropolymer supports for use as a reaction catalyst. Moreover, consistently high rate of conversion during catalyst re-use over time is achieved. Furthermore, inactive enzyme catalyst can be stripped from the support and fresh enzyme can be bound to the support to achieve the original conversion rate. The immobilization of catalytic enzymes on porous fluoropolymers is a viable and novel technology for the preparation of advantaged catalysts.

Disclosed is an integrated process comprising first and second biotechnical processes. The first process produces biofuel and / or starting material for biofuel and uses a microorganism capable of producing enzymes. The second process produces biofuel and / or starting material for biofuel. In the integrated process, the microorganisms are cultivated and biofuel and / or starting material for biofuel and enzymes are produced. The microorganism culture, supernatant or a protein enriched fraction or a dilution of the supernatant comprising catalytically active enzyme(s) are introduced into the first and / or into the second process, or feedstock for the process(es) is treated. The invention relates also to use of the produced enzymes in biofuel production process or in other applications as an enzyme preparation or a source of enzymes. The invention relates also to use of the produced lipids and alcohols as biofuel, a component of biofuel or a starting material for biofuel production.

The invention discloses bacillus megatherium, a method for preparing a microbial inoculum through solid fermentation of the bacillus megatherium and an application of the microbial inoculum, belonging to the technical field of microbial preparations. The bacillus megatherium JSSW-JD-2F5 has been preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC NO.8045. By adopting solid fermentation, a preparation method of a bacillus megatherium JSSW-JD-2F5 solid fermentation microbial inoculum and the application of the solid fermentation microbial inoculum are disclosed, wherein the solid fermentation microbial inoculum is used as an additive of a livestock, poultry and fishery cultivation feed and is added into the feed according to a mass percentage of 0.01-0.05%. The product of the microbial inoculum contains high-concentration viable bacteria, active enzyme and physiological active matters, and the concentration of the viable bacteria reaches more than 1.0*10<10>CFU / g and can meet requirements of a feeding microbial additive on the viable count; the application of the inoculum not only can improve the breeding environment, but also has an important significance for increasing the feed utilization ratio and promoting the growth of animals, so that the inoculum is widely applied to livestock, poultry and fishery cultivation.

Activity-based probes, which are specific for certain active cysteine proteases (caspase, cathepsin and legumain) and carry radioactive labels, are disclosed. The present probes comprise an acyloxymethyketone (AOMK) “warhead” that binds only to active enzyme. The probes further comprise peptide-like structure that targets the probe to a specific cysteine protease or protease family, and a radiolabel on the probe, which is bound to the targeted enzyme. It has been found that the present probes are stable in vivo and give specific target images distinguishable over background. The preferred probes are labeled with a positron-emitting agent such as 64Cu, 125I (SPECT) and 99mTc (PET). The probes show in vivo half-life and stability well suited for imaging.

The present invention provides, inter alia, fluorogenic enzyme substrates, such as fluorogenic polypeptide substrates, libraries of fluorogenic enzyme substrates and methods for assaying for enzymatically active enzymes, such as hydrolases (e.g., proteases), in biological samples.

The invention belongs to the technical field of microorganism-fermented traditional Chinese medicines, and provides a method for preparing a composite-probiotic-fermented Chinese herbal medicine in order to meet the current probiotic diet therapy healthcare requirements and solve the problem that active ingredients are broken by a high temperature during the Chinese herbal medicine preparation. The method comprises the following steps of: respectively preparing a Chinese herbal medicine culture medium and composite probiotic strains; adding the composite probiotic strains into the Chinese herbal medicine culture medium based on 35-42% of gross amount of the Chinese herbal medicine culture medium, sealing and fermenting, and controlling a fermentation temperature to 28+ / -2 DEG C and fermentation time to 30 days; adding 10 times of water after fermentation, stirring and leaching, separating leaching liquid, standing for precipitating, separating a clear solution, and obtaining the finished product of the composite-probiotic-fermented Chinese herbal medicine. The method for preparing the composite-probiotic-fermented Chinese herbal medicine, disclosed by the invention, avoids the breakage of the high temperature to the active ingredients, reserves active enzymes, vitamins and the like to the maximum extent, and also reserves a great amount of live probiotics, bacteroid secretion and bacteroid autolysate by utilizing normal-temperature solid fermentation; and no three wastes are discharged during preparation.

The invention applies to the tobacco crop growth, which can improve the soil structure and provide adequate moisture, oxygen and nutrition to improve the yield and quality in tobacco crop cultivation. The invention relates to a fertilizer containing microorganisms, organic matters and inorganic matters. The fertilizer adopts biological technology and electrolysis technology which complement each other, takes micro-organisms and mineralized substances as additive, as well as combines with nutritional formula (nitrogen, phosphorus, potassium, sulfur, magnesium, calcium, silicon, boron, zinc, iron, molybdenum, bacterial protein, amino acid, humus, plant nucleic acid, B vitamins, active enzymes and unknown factors which promote crop growth), so as to provide adequate nutrition for crops and improve soil to make the soil conducive to the cultivation of tobacco crops. Through biotechnology, the micro-organisms in a dormant state are added to the fertilizer and are activated under favorable environmental conditions to decompose the animal and plant residues, pesticides, fertilizers, heavy metal residues and minerals in the soil so as to change the contents of nitrogen, phosphorus and potassium. Through electrolysis technology, the mineralized substance additive enables the soil to produce a chain reaction to moisture and oxygen supply, so as to gradually change the ion charge in the soil, increase the soil oxygen supply amount, improve the moisture infiltration capacity and strengthen the conditions for microbial activities.

The invention discloses a fruit flavor for cigarettes and application of the flavor. The fruit flavor for cigarettes is prepared by naturally fermenting, filtering, centrifuging, and freeze-drying edible fruits and sugar as raw materials. The fruit flavor is rich in active enzyme, reducing sugar, amino acid, vitamin, trace elements and minerals and the like and can be used in tobacco leaf or shredded stem feeding process. The fruit flavor for cigarettes is simple to manufacture, safe and environmentally friendly and has good application prospects; by the fruit flavor, the internal quality of the cigarettes can be significantly improved.

The invention discloses an animal feed additive and a preparation method thereof. The animal feed additive is prepared from the following raw materials in parts by weight: 5-10 parts of corn fiber, 15-20 parts of soybean curb residue, 12-15 parts of zeolite powder, 40-60 parts of corn sugar residue, 1 part of compound biological active enzyme, 1 part of saccharomycetes, 1 part of lactobacillus, 1 part of bacillus and 1 part of bifidobacterium, wherein the compound biological active enzyme comprises the following components: 20,000 IU / g of xylanase, 10,000 IU / g of pectinase, 400 IU / g of cellulose and 200 IU / g of protease. The preparation method comprises the steps of raw material weighing, pre-processing, mixing, dual fermentation and packaging. The animal feed additive disclosed by the invention can be used for improving the digestibility to promote the growth and development of animals, regulating the gastrointestinal micro-ecological balance to enhance animal immunity and improve disease resistance, improving animal feed intake, improving the cultivation environment to reduce respiratory diseases, and improving the quality and quantity of cultivated products.

The invention relates to a thin-film material for an optical fiber biosensor probe and a preparation method thereof. The material has catalytic characteristic and photosensitivity and is made from Fe3O4 / SiO2 magnetic nanocomposite particles, bioactive enzyme, fluorescent material and sol-gel. The bioactive enzyme is fixed on the Fe3O4 / SiO2 magnetic nanocomposite particles and the fixed bioactive enzyme and the fluorescent material are embedded in a sol-gel grid. The preparation method comprises the following steps: a Fe3O4 / SiO2 magnetic nanocomposite particle carrier is synthesized; the carrier is activated by glutaraldehyde or by glutaraldehyde, bovine serum albumin and glutaraldehyde in sequence and then has a crosslinking reaction with the bioactive enzyme to fix the enzyme; the Fe3O4 / SiO2 magnetic nanocomposite particles fixed with the bioactive enzyme are dripped on the surface of a treated glass substrate after being mixed with the sol-gel liquid blended with a Ru(bpy)3Cl2 fluorescent indicator; after cleaning, drying and aging, a sol-gel optical bio-composite sensing film embedding the bioactive enzyme and the fluorescent indicator is obtained. The optical fiber biosensor probe made from the thin-film material is especially applicable to the detection of blood sugar, blood cholesterol and the like.

The invention discloses a method for preparing pinctada martensii high F value oligopeptide by an enzymatic method, relates to enzymatic hydrolysis of a pinctada martensii meat protein and desorption of an aromatic amino acid in a hydrolysate by adopting an effective separation means, and provides a method for preparing an oligopeptide mixture with the molecular weight of less than 2,000 Da and the F value (the molar ratio of a branched chain amino acid to the aromatic amino acid) of more than 20 by adopting the enzymatic hydrolysis of the pinctada martensii meat protein and an adsorbent adsorption and separation desorption effect. In the method, an aquatic animal protein is first taken as a raw material to prepare the high F value oligopeptide; the raw material is extracted by using hot water in a preparation process, the extract can be used for extracting taurine, and the precipitated protein is taken as an enzymatic substrate, so that the resource is utilized fully and effectively; a single enzyme, namely pancreatin is used as the preferred active enzyme; and the aromatic amino acid is desorbed by adopting a method of combining activated carbon static adsorption and gel filtration chromatography to prepare a high F value oligopeptide product.

The invention provides a method for preparing hydrophobic vitamin microcapsules, and relates to vitamin microcapsules. The method comprises the following steps: (1) adding an oil-phase core material in an agitating pan, and melting the oil-phase core material to prepare vitamin oil containing antioxidant, wherein the oil-phase core material comprises lipid-soluble vitamin, an antioxidant and other greases; (2) dissolving a wall material in water to prepare a water solution with solid content of 20-60wt% to obtain a water phase; (3) adding the vitamin oil containing antioxidant prepared in the step (1) into the water phase obtained in the step (2), and emulsifying and homogenizing to form uniform emulsion; (4) adding protein active enzyme into the emulsion prepared in the step (3), granulating, performing a crosslinking reaction, and drying to obtain the hydrophobic vitamin microcapsules. According to the method, other toxic or strong oxidizing chemical substances are not added, the prepared microcapsules are safer and more environment-friendly, has longer shelf life, and is high in embedding rate and low in surface oil, so that the microencapsulation efficiency can be further improved.

A produce-treatment composition is provided. According to an embodiment, the composition contains a nonionic surfactant, an antimicrobial agent, and a botanical extract from a plant selected from the Liliaceae and Cactus families, the extract retaining active enzymes and amino acids of the plant. Methods of treating produce and produce-handing and storage equipment are also provided.

The invention relates to a method for extracting type I collagen. The method comprises the following steps: cleaning bovine tendon, refrigerating and slicing; disinfecting and degreasing the sliced bovine tendon with 75-percent alcohol and normal hexane; adding a proper amount of active enzyme into homogenate for performing enzymatic digestion after removing impure proteins and smashing the homogenate; performing salting-out and dialysis treatment on supernatant; performing freeze drying and vacuum packaging on a collagen stock solution obtained after the dialysis to obtain a type I collagen product. The method is simple in preparation process, can be used for realizing large-scale production, and is an economical and effective extraction method. The type I collagen extracted by the method has superior biocompatibility and bioactivity, can be applied to biomedicine, and has an important application value in the field of makeups; meanwhile, epithelial cells can be activated, the hyperplasia of the epithelial cells and the generation of collagenase are facilitated, and the skin becomes tight and elastic.

The invention belongs to the technical field of health-care food, and relates to compound combined rape pollen chewable tablets and a preparation method thereof. The preparation method comprises the following steps of: crushing and screening a mixture of rape pollen, soybean lecithin, Chinese angelica extract, royal jelly freeze-dried powder, tea polyphenol, vitamin, soft sugar and microcrystalline cellulose, preparing the mixture into a soft material, then performing granulation and drying, performing granulation again to form granules, finally mixing the granules and magnesium stearate, pressing the granules and the magnesium stearate into tablets of different forms through a tablet press, and performing damp-proof packing. The soybean lecithin, the bee pollen, the Chinese angelica extract, the royal jelly freeze-dried powder and the like have complementary and additive effects; the chewable tablets can be directly chewed, are convenient for digestion and absorption, and have good taste; and the chewable tablets have unique effects of improving sleeping and removing chloasma at the same time of increasing multiple nutritional components, active enzymes and trace elements for the human body.

The invention relates to a corn husk tobacco sheet and a production method thereof. The production method adopts the following steps: (1) the biodegradation and the extraction treatment are performed, wherein a. when a corn husk is heated to 55 DEG C in water, active enzyme is added; b. the mixture reacts for 2 hours at a temperature of 60 DEG C, and alcohol with the same amount of the water is added; c. biological enzyme is inactivated at a temperature of 90 DEG C to reclaim the alcohol; and d. the corn husk is dehydrated, and reaction extraction liquid is condensed to be pasty; (2) the corn husk after the treatment is produced into paper pulp; (3) the paper pulp is produced into a corn husk sheet; and (4) the pasty extraction liquid is coated on two surfaces of the corn husk sheet and is subject to drying treatment. The corn husk tobacco sheet has the advantages that the corn husk tobacco sheet is blended into tobacco to produce cigarettes, which can provide harmonious aroma for the cigarettes and excite sweet gas of smoke gas; and the corn husk tobacco sheet at least can substitute more than 5 to 15 percent of the using amount of the tobacco, and effectively reduce the smoke gas volume and harmful ingredients of one cigarette.

Methods for the production of purified, catalytically active, recombinant memapsin 2 have been developed. The substrate and subsite specificity of the catalytically active enzyme have been determined and were used to design substrate analogs of the natural -2 substrate that can inhibit the function of memapsin 2. Processes for the synthesis of two substrate analogues including isosteres at the sites of the critical amino acid residues were developed and the substrate analogues, OMR99-1 and OM99-2, were synthesized. The inhibition constant of OM99-2 is 1.6×10−9 M against recombinant pro-memapsin 2. Crystallography of memapsin 2 bound to this inhibitor was used to determine the three dimensional structure of the protein, and the importance of the various residues in binding. This information is useful for designing new inhibitors to memapsin 2, for diagnosing and treating and / or preventing Alzheimer's disease.

The invention discloses an aspergillus niger strain and use thereof. The aspergillus niger strain is aspergillus niger A46CGMCC No.3084. The strain can produce xylanase, cellulose, pectinase, amylase, beta-mannase and the like with high yield at the same time of xylanase, cellulose, pectinase, amylase, beta-mannase and the like at the same time, as well as an enzyme complex for feeding animals, wherein the optimum pH for active enzymes in the enzyme complex is between 4 and 6 which meets the requirements of the gastrointestinal tracts of the animals. The enzyme complex for feed use can be widely used in livestock feed to reduce anti-nutritional factors, improve the digestibility, create excellent economic benefits and promote ecological environment protection and the sound development of the livestock industry.

The invention relates to a method for producing complete biological feed by utilizing edible mushroom residue by way of composite microorganism starter solid fermentation, which includes the process steps of material processing, composite microorganism starter preparation, solid fermentation, drying, grinding and the like. The complete biological feed produced by the method does not have any chemical additive ingredients, has natural fermentation aroma, contains a variety of amino acids, vitamins and other nutrient substances and a great deal of biological active enzyme, can greatly reduce the contents of glutamic-pyruvic transaminase, glutamic-oxalacetic transaminease, cholesterol, triglyceride and urea nitrogen in the blood and improve the quality of animal livers and kidneys, and is a high-quality, cheap green biological complete feed for feeding livestock.

The invention relates to a microorganism multienzyme additive for promoting the growth and development of baby pigs, which is characterized in that the additive is formed by composite synchronizing solid fermentation of a composite strain stock solution, carbon source, nitrogen source, inorganic salt and enzyme revulsant. A manufacturing method is that all the strains adopt a composite inducing technology to carry through filtering; a method of synchronizing liquid culture and solid composite culture is adopted to lead all the strains to be cultivated in the same culture medium; the total number of live bacterium reaches 3 billion / g and simultaneously a fermented product is kept completely. The product comprises massive physiological metabolism substances like active enzyme, small peptide, oxidation resistance substances, FAA, vitamins, etc. A specific type for feed preparing particles carries through a double-layer microcapsule peridium treatment on the strains that can not resist heat. The invention has the advantages of promoting the digestive absorption on the feed by the baby pigs, promoting the compound of organism protein and full utilization, increasing the disease resistance and immunological competence of the baby pigs, improving the feed switching rate and the production capacity of cultivated animals, reducing the pollution to the environment caused by animal dejecta. The invention is a green and safe agrotechnical product.

The invention relates to the microbiological engineering technology. Crop byproducts, such as agricultural straws, hulls and cakes and the like are utilized to produce high protein feed and animal promoters by multiple strains to lead wastes to be effectively utilized; waste materials are changed into valuable things, so that raw materials are inexhaustible, thus improving input-output ratio of feed with significant economic and social benefits. As containing a great number of active enzymes and growth promoting factors, the technology improves the function of animal metabolism, and leads theactive enzymes and growth promoting factors to generate mutual synergism and comprehensive balance to animal organisms, thus ensuring that nutrient substances can be rapidly degraded in animal intestinal canals, promoting animal to absorb and transform nutritious ingredients in the feed, and achieving the purposes of promoting animal growth and saving feed. The technology is mainly applied in animal feed promoter, such as pigs, chickens, cows and sheep and the like and biological high protein feed. The invention has easily mastered production method and process, less investment, low cost and is suitable for investing and building factories for individuals and medium-sized and small enterprises.