The invention discloses a tissue cultivation method of bulbus fritillariae cirrhosae. Fritillaria cirrhosa D.Don is a Liliaceae Fritillaria perennial medicinal herb, underground bulbs are used as medicine, the bulbus fritillariae cirrhosae has the effects of clearing away heat and moistening the lungs, preventing phlegm from forming and stopping coughing, and is one of the main sources for the famous traditional Chinese medicinal material bulbus fritilariae specified in the Chinese Pharmacopoeia (Year 2005). The bulbus fritillariae cirrhosae is distributed in high altitude localities, is propagated through seeds, and is low in reproductive rate and long in growth cycle; due to long-term harvesting and wild resource deficiency, the problem of large market demand needs to be solved urgently. Bulbs of the bulbus fritillariae cirrhosae are used as explants, bulbus fritillariae cirrhosae in-vitro regenerated plants are obtained through the processes of explant sterilization, induction of callus tissues in two different states, bulb differentiation, multiplication, rooting culture, acclimatization and transplantation and the like, an effective tissue culture rapid propagation system is established, a large number of seedlings can be produced in a short period of time, the breeding efficiency is improved, and the propagation and popularization of the bulbus fritillariae cirrhosae are facilitated.