914 results about "Low temperature treatment" patented technology

The invention provides a method for extracting DHA from cells of algae and fungi by breaking cell walls and relates to a method for extracting active ingredients from microorganisms. The method provided by the invention avoids the assistance from any organic solvent and chemical medicaments, breaks the walls of the cells of the algae and the fungi by using a physical method and extracts a grease product DHA (docosahexaenoic acid) from the cells and is suitable for large-scale production at low cost. The method comprises: after fermentation is finished, introducing fermentation liquor of microalgae or fungi to a separation system for separating and collecting the cells, adjusting the pH value of bacterial sludge with an acid to 2.0 to 4.0, keeping the temperature of the bacterial sludge between 10 and 20 DEG C, adding antioxidant into the bacterial sludge, and performing high-pressure homogenization and wall breaking by using a high-pressure homogenizer; and adding water into the bacterial sludge subjected to wall breaking, stirring the bacterial sludge, and separating feed liquor in a three-phase separator to obtain DHA grease. In the invention, physical wall breaking and the physical extracting method are used, the process is simple, the cell breaking is high efficient, the low-temperature treatment and antioxidant treatment of the bacterial sludge can effectively protect thebioactivities of the matters in the cells of the algae and the fungi, and the product is green, nontoxic and free from residues.

An amorphous hard carbon film has low friction coefficient from the beginning of sliding, when the metal oxide is contained in the film. The adhesion of the film to the substrate is also improved due to the low temperature treatment of RF plasma enhanced CVD method. This film can be applied to many sliding members, e.g., a piston ring, a vane of compressor and a plunger of fuel injection pump, used under severe condition.

The invention discloses a high specific capacity spindle-shaped ferroferric oxide / carbon nano composite material for a negative electrode of a lithium ion battery. A raw material, namely soluble ferric salt is solved in a mixed solvent of glycerol and distilled water; after low-temperature treatment, precipitates are collected, and a spindle-shaped iron oxide hydroxide (FeOOH) precursor is obtained; and after the precursor is subject to carbothermal reduction, the ferroferric oxide / carbon nano composite material with the same appearance is obtained. The invention discloses a preparation method of the spindle-shaped ferroferric oxide / carbon, which has the advantages that the operation is simple, convenient and simple to do, the used raw material has a cheap price, mass production is facilitated, and the synthetized spindle-shaped ferroferric oxide / carbon nano composite material shows high first time coulomb efficiency and high specific capacity when being used as the negative electrode of the lithium ion battery, and is a promising negative electrode material of the lithium ion battery.

A microhollow cathode discharge assembly capable of generating a low temperature, atmospheric pressure plasma micro jet is disclosed. The microhollow assembly has at two electrodes: an anode and a cathode separated by a dielectric. A microhollow gas passage is disposed through the three layers, preferably in a taper such that the area at the anode is larger than the area at the cathode. When a potential is placed across the electrodes and a gas is directed through the gas passage into the anode and out the cathode, along the tapered direction, then a low temperature micro plasma jet can be created at atmospheric pressure. Selection of gas microhollow geometry and operational characteristics enable the application of the assembly to low temperature treatments, including the treatment of living tissue.

A method for extracting bean phytohemagglutinin belongs to the extraction technical field of useful constituents of plants. The method is a water extraction method in which the extraction is performed at 40-60 DEG C based on the following steps: crushing bean seeds to 40-100 meshes, adding soak solution to extract bean husk at the temperature of 40-60 DEG C for 60-180min and then fish the bean husk, performing rough separation to remove bean dregs, centrifugally separating to obtain supernatant with the bean phytohemagglutinin, further performing separation and purification on the supernatant, condensing or drying the supernatant to obtain the phytohemagglutinin. Ultrasonic wave or microwave can be exerted in the whole or part of the extraction process, or the ultrasonic wave and the microwave are alternately exerted to perform auxiliary extraction. A traditional low-temperature soaking and low-temperature treatment method is completely discarded in the method, and practice proves that the method is a simple and efficient method for extracting the phytohemagglutinin in short time. Related detection proves that the obtained phytohemagglutinin can meet conventional experimental needs and medicinal needs. The method helps overcome the disadvantage of more chemical reagent composition residue and long extraction time of the products obtained by the prior art.

The invention relates to a producing method of a teflon spatial structure membrane comprising following steps: fiberglass cloth low temperature genapping treatment, fiberglass cloth bottom layer dipping treatment, structure layer dipping treatment, surface layer dipping treatment and sintering treatment. Low temperature high speed genapping, multitime coating low-temperature treatment, and once hyperthermia flash sintering effectively reduce tensile strength descending caused by high-sintering treatment. Since fiberglass cloth is used as backing material, dipping treatment is not needed to be performed on the fiberglass yarn, the procedure is simplified to facilitate the production thereof, and shortcomings in prior art are overcome which are modified fiberglass yarn weaving difficulty and intensity and plasticity descending caused by multitime sintering. The teflon spatial structure membrane produced by using the invented method has a bottom layer, a structure layer and a surface layer, is provided with high pulling-against intensity, high tearing-against intensity, anti-aging property, good flexibility, good light-admitting quality and a long service life, and can be used widely in air stations, gymnasiums, exhibition halls, large-scale factory buildings and factory building warehouses.

The invention relates to a process method for improving the manufacturing accuracy of an aluminum-alloy open-type integral impeller. The method comprises the concrete steps of preparing materials, carrying out rough-turning on impeller blank shape, carrying out rough-turning on a process gripping head, carrying out heat treatment, carrying out artificial aging, carrying out semi-fine-turning on the process gripping head, carrying out semi-fine-turning on impeller shape, carrying out low-temperature treatment, carrying out five-axis-linkage-machining rough-milling, carrying out natural aging, carrying out five-axis-linkage-machining fine-milling, gripping, carrying out fine-turning on an axle hole, carrying out fine-turning on an impeller, gripping and carrying out dynamic balance. The impeller manufactured by adopting the process method provided by the invention has the characteristics that the shape is complete, the curved face is smooth and unhindered, blade curved faces and flow passage curved faces are low in deformation, and shown by the results of a fluorescent flaw detection, blade surfaces and front edges of the blades are flawless; during the dynamic balance of the integral impeller, mass reduction is relatively low, the qualified rate is high, and the qualified rate of excess-revolution tests of the integral impeller is greatly increased; the improvement of process for the series of integral impeller parts is realized, and good product quality, performance and design requirements are guaranteed; meanwhile, the stability of production quality is improved, and the reliability of products is improved.

The invention belongs to the technical field of preparation of semiconductor thin films, and particularly relates to a low-temperature treatment method for producing an indium gallium zinc oxide (IGZO) semiconductor thin film by using a sol-gel method. The method comprises the following steps of: dissolving In(NO3)3.4*5H2O, Ga(NO3)3.4*5H2O and Zn(C2H3O2)2*5H2O into ethylene glycol monomethyl ether serving as a solvent and monoethanolamine serving as a stabilization agent to form a clear stable precursor solution; and coating the precursor solution on a glass substrate in a rotatable manner, and irradiating by using an infrared heating lamp to obtain the flat and transparent IGZO semiconductor thin film. Compared with the conventional method for preparing the IGZO thin film through annealing of a heat plate by using the sol-gel method, the method has the advantages that the IGZO thin film irradiated by the infrared heating lamp is relatively high in semiconductor and optical properties, and the process temperature is relatively low and lower than 250 DEG C; the IGZO thin film is used as a thin film transistor with a trench layer material; the switch current ratio is more than 5*10<6>; the saturated migration rate is more than 1.8 cm<2> / Vs; and the subthreshold amplitude is less than 2.2 V / dec.

A system for producing Erbium Oxide thin films with increased photoluminescence. The system includes a depositing stage for forming Erbium Oxide molecules by reacting Erbium sputtered atoms with O2 in a gas phase and creating the Erbium Oxide thin film by depositing the Erbium Oxide molecules on a substrate coated with Silicon Oxide. The system further includes an annealing stage for annealing the Erbium Oxide thin films by utilizing a low temperature treatment for a specified amount of time and temperature followed by a high temperature treatment for another specified amount of time and temperature, wherein the crystallinity of the thin films has improved.

The invention discloses a preparation method for a composite hard carbon negative electrode material. The preparation method comprises the following steps: 1) subjecting asphalt to cross-linking polymerization so as to obtain a cross-linked polymer; 2) successively subjecting the cross-linked polymer to curing, crushing, grading and precarbonization low temperature treatment so as to obtain a carbon precursor; 3) crushing and grading the carbon precursor and then carrying out high temperature carbonizing treatment so as to obtain a carbonized particle; and 4) uniformly mixing the carbonized particle with natural graphite according to a mass ratio of 2: 3 to 4: 1. The preparation method provided by the invention has the advantages of simple and easily practicable process, wide sources of raw materials and low cost. The invention further discloses the composite hard carbon negative electrode material and application thereof. The composite hard carbon negative electrode material has initial discharge capacity of more than 300 mAh / g, initial charge and discharge efficiency of more than 85%, good heavy-current charge and discharge performance, good electrochemical performance, good cycle performance, good heavy-current charge and discharge performance, good security, good adaptability to an electrolyte and other additives and stable properties, and different batches of the prepared material barely show a difference.

The invention discloses a preparation method of an astaxanthin micro nanometer solid steady-state preparation capable of being dispersed through cold water. A natural astaxanthin extract is used as a raw material, through technologies of low-temperature treatment, low-energy self micro emulsification, microencapsulation, drying, granulation and embedding twice and the like, the astaxanthin micro nanometer solid steady-state preparation capable of being dispersed through cold water can be prepared. The prepared astaxanthin solid preparation powder disclosed by the invention is dried, good in flow property, and good in product processing and storing stability; and besides, the bioavailability of astaxanthin is significantly improved. The method is simple in preparation technology, mild in condition, and suitable for industrialized popularization and application. Through the adoption of the technique of the preparation method disclosed by the invention, the astaxanthin can be steadily applied in the field of aqueous-phase medium foods, solid medium foods, various premix food, daily chemical articles for use, medicines and the like, the application field of the astaxanthin is greatly widened, the pluralism of products is greatly widened, and the market potential is tremendous.

The invention relates to a skate liver oil and a preparation method thereof. The skate liver oil is characterized in that the skate liver oil comprises 0.76-1.13mass% of alkoxy glycerin, 0.71-0.92mass% of squalene, 25-28mass% of EPA and DHA, 1200-1600IU / g of vitamin A, 120-150IU / g of vitamin D, and the other substances which are lipids. The preparation method comprises the following steps: freezing skate livers, unfreezing, chopping, carrying out enzymatic hydrolysis treatment, carrying out enzyme killing treatment after the enzymatic hydrolysis, centrifuging, freezing the obtained emulsion, unfreezing, demulsifying, centrifuging, refining, degumming by citric acid, deacidifying by a caustic soda-soda ash mixture (a mass ratio of caustic soda to soda ash is 2:1), carrying out low temperature treatment, and carrying out evacuating nitrogen-filling packaging. The method has the advantages of reasonable operation, low cost and mild conditions, and the obtained skate liver oil has a very low peroxide value, a very low acid value and no nutrition loss, is rich in vitamin A, vitamin D, EPA, DHA, and important squalene and alkoxy glycerin, has a reinforced healthcare effect, and is easy to preserve and circuit in the market because of the evacuating nitrogen-filling packaging.

The invention relates to a cultivation method for an oriental lily fresh cut-flower, which comprises the following steps of: carrying out different stages of rooting and low-temperature forced germination on handpicked refrigerated seed balls, then planting in a transformed ground substance, strictly controlling the illumination, the temperature, the humidity of each growing stage, and evenly applying all kinds of required fertilizers. In the invention, through low-temperature treatment in a refrigeration house before planting, the roots of the lily seed balls grow to a certain extent, the influence of the harmful factors, such as high temperature and the like, on root system growth in the growth process after planning is reduced, thereby ensuring the supply of the nutrient and the moisture for the plant, and the quality of the cut-flower is improved. By using the cultivation method, the oriental lily has the advantages of fine growth and development, high and robust plant, high cut-flower rate, excellent quality, long childbearing period, bright-coloured flower color, bright lamina, thick and rigid petal character, good insertion-resistance, and little appearing leaf burning; the cultivated lily plant has strong disease resistance and aromatic flavor; and the cultivation method has high use ratio of facility space and can shorten the growth cycle of the fresh cut-flower.

The invention relates to a cultivation method of crops, and particularly relates to a cultivation method of broccolis and the method belongs to the technical field of plant cultivation. According to the invention, on the basis of the existing cultivation technologies, lucifugal low-temperature treatment is carried out on germinated broccoli seeds, so that the green vernalization of the broccolis can be promoted; and on the basis of accelerating each state of the flower bud differentiation, the leafing period is brought forward, benefit is brought to the improvement of photosynthetic products and the aim of promoting the high yield of the broccolis is achieved. The cultivation method has the beneficial effects that simplicity and operability are provided, the mature period of the broccolis can be brought forward by about 3 days, the yield is increased by more than 10% compared with the conventional cultivation, and the comprehensive benefit is higher.

The invention relates to a rice anther culture improvement technology, and in particular relates to a method for improving the frequency of callus induction and plant differentiation rate in japonica rice anther culture. The method comprises the following steps that: 200g / 667m<2> of 10% of paclobutrazol wettable powder and 50-60L of water are mixed for spraying treatment before sampling, the spraying of the paclobutrazol and low-temperature treatment cause multiple pollen cells in the anther to be in the later monokaryotic stage, novel cytokinin 4-FA is introduced into a callus inducing medium, under the synergistic action of the 4-FA, KT, and 2,4-D, division is easily started in the dark culture after inoculation, and the frequency of callus induction is greatly improved. By using the anther culture method, the frequency of callus induction in the japonica rice anther culture is obviously improved to exceed 85.0%. A differential medium comprises the following ingredients: an MS (Murashige and Skoog) basic culture medium, 0.5mg / L of NAA, 0.5mg / L of ZTO, 0.8mg / L of TDZ, 0.2mg / L of FA, 0.2mg / L of MET, 30g / L of saccharose, and 8g / L of agar powder; and after the novel hormones of ZT, 4-FA and TDZ are introduced, light cultivation is carried out, and plant differentiation rate can reach more than 86%.

The invention discloses a flowering time adjustment and control method for enabling a peony to blossom in winter. The method comprises the steps of variety and plant selection, forced dormancy, root growth promoting and flower forcing treatment, so that a purpose of manually advancing the flowering time of the peony is achieved. The flowering time of the peony is advanced by performing low-temperature treatment on the peony in summer for breaking the dormancy; a purpose of effectively controlling the flowering time is achieved by controlling the temperature and illumination during flower forcing; the treated peony is large and bright-colored; a peony plant is robust; the flowering time is longer; and an ornamental value is high.

The invention relates to a shape memory resin material for 3D printing, and a preparation method and application thereof. The resin material is prepared from the following raw materials in parts by weight: 70-95 parts of polycaprolactone, 4.5-5.5 parts of polyurethane, 4.5-5.5 parts of inorganic filler, 1.5-2.5 parts of crosslinking agent and 0.5-1.5 parts of surfactant. The shape memory resin material for 3D printing has a shape memory function in the presence of warm water; a medical product prepared from the shape memory resin material can be taken down from and put on a human body at a low human body tolerable temperature according to needs, the thickness is adjustable, and the flexural rigidity can be adjusted by changing the thickness of a formed film; meanwhile, the shape memory resin material can be shaped randomly by being soaked at 50 DEG C or below, being blown by a hot-air fan and being irradiated with near infrared light; and after being subjected to low-temperature treatment at 50 DEG C or below, multiple polymer films can be superposed randomly, thereby dimensionally forming a whole.

The invention discloses a composite preservation method of hairtail slices by partial freezing treatment in combination with a natural preservative, which comprises natural preservative dipping treatment and partial freezing low-temperature treatment. The invention can achieve the effects of inhibiting bacteria and lower the temperature of fish slices. The natural preservative comprises the following components in percentage by weight: 0.02-0.05% of nisin, 0.01-0.06% of epsilon-polylysine, 0.01-0.05% of lysozyme and the balance of water. The partial freezing process for preserving the product ensures the integrity of the product cells, can maintain the original color, freshness and flavor, greatly prolongs the shelf life of the product; and the product is still very fresh after being stored for 25 days.

The invention discloses a new method for inducing a garlic test tube bulb. The method mainly comprises the following steps of: performing low-temperature treatment of the garlic tender bud at 4 DEG C to induce atmogenic bulbil; rejuvenating atmogenic bulbil on an MS+2.5mg KT+0.5mg NAA culture medium; and inducing the test tube bulb on an MS+60g / L sucrose culture medium without hormone. The method can be used for improving the propagation efficiency of garlic rapid propagation by using atmogenic bulbil as seeds, and reducing the cost. By virtue of the method disclosed by the invention, the atmogenic bulbil induction rate can reach 100% (each bud can induce out atmogenic bulbil), each bud can induce out 25 atmogenic bulbils, all of the atmogenic bulbils can be induced into test tube bulbs, and the test tube bulb induction rate reaches 100%. Without cell dedifferentiation and redifferentiation in the whole induction process, the genetic mutation probability is reduced; and the method disclosed by the invention provides important possibility for the industrialized seedling production of garlic rapid propagation.

The invention provides a technology for cultivating super-early-maturing pollution-free strawberries. The technology mainly comprises the steps of seedling cultivation, high-temperature soil treatment, low-temperature strawberry seedling treatment, field planting and management conducted after field planting. The technology for cultivating super-early-maturing pollution-free strawberries has the advantages that, because during cultivation and planting, application of pesticide is reduced to a great extent, the strawberry taste and the strawberry quality are improved, and the harm of pesticide residuals to a human body is reduced at the same time; meanwhile, due to the fact that the manual low-temperature treatment method and the short-day treatment method are adopted, flower bud differentiation of strawberries is accelerated, the indoor temperature rises, the maturing of strawberry is accelerated, and the life demands of people are met.

The invention discloses a method for screening the cold-resistant variety of steeplebush and relates to a method for screening the cold-resistant variety of a garden tree, namely the steeplebush. The invention provides the method for screening the cold-resistant variety of the steeplebush, a technical system and the method for screening the cold-resistant variety of the steeplebush are established in advance for the first time, and the technical blank about the cold-resistance screening of the steeplebush at home and abroad is filled. The method comprises the following steps of: 1, performingoutdoor natural low-temperature treatment on the steeplebush; 2, performing persistent constant and low-temperature treatment on the steeplebush; 3, performing outdoor natural freezing treatment on the steeplebush; 4, calculating subordinate function values of cold-resistance physiological indexes; 5, performing simulative outdoor low-temperature gradient freezing treatment on the steeplebush, and calculating the semilethal temperature of the steeplebush; and 6, determining the cold resistance of the steeplebush according to the average subordinate function value and the semilethal temperature of the steeplebush so as to finish the screening of the cold-resistant variety. In the method, double results, namely the subordinate function values and the semilethal temperature of the cold resistance are combined, so that the simplicity of the cold-resistance screening is overcome, and the cold-resistance screening is highly consistent with the overwintering performance of the steeplebush. The invention provides technical support for screening cold-resistant steeplebush in northern gardens and is applied to the field of the cold-resistance screening of the steeplebush.

The invention relates to a high-strength strain-sensitive polyvinyl alcohol ion hydrogel sensing material, and a preparation method and application thereof. The hydrogel is obtained by fully mixing polyvinyl alcohol with glycerin and deionized water while stirring under strict temperature control conditions, and soaking the mixture in a saturated sodium chloride solution after low temperature treatment. The low temperature treatment promotes formation of a large number of hydrogen bonds between the PVA and the glycerin, and the soaking treatment by the saturated sodium chloride solution promotes entanglement among PVA chains, and interaction between two physical effects of hydrogen bonding and chain entanglement remarkably improves mechanical properties of the hydrogel. Since Na+ and Cl- ions exist in the hydrogel, a hydrogel sensor has a high sensitivity coefficient and can repeatedly and stably detect changes of electrical signals. The synthesis process of the hydrogel sensor is simple to operate and easy to control, raw materials are cheap and easy to obtain and have high biocompatibility and nontoxicity, and a wearable device produced from the hydrogel sensor can safely and effectively monitor the movement of each part of the human body.

The invention discloses a preparation method of a liquid acidic pectinase, belonging to the technical field of enzyme preparations. The preparation method of the liquid acidic pectinase comprises the steps of treating a fermented crude enzyme solution and a blended concentrated solution at normal temperature by using a high-voltage pulsed electric field, and adding a pectin decomposer, a Chinese herb extract, modified dietary fibers and other natural plant raw materials in a blending process, wherein chemical preservatives do not need to be added, and low-temperature treatment is not needed; and then, degerming, filtering, and carrying out sterile filling to obtain the liquid acidic pectinase with stability and long shelf life. Experiments prove that the total bacterial count is 68-102CFU / mL if the liquid acidic pectinase prepared by using the preparation method is stored at room temperature for 12 months; and the enzyme activity loss ratios are respectively 1-1.4% and 0.5-1.1% if the liquid acidic pectinase is stored at 40 DEG C and 0 DEG C for 12 months. The preparation method is simple in process, convenient to operate, energy-saving and environment-friendly.

The present invention provides block copolymer films for application to surfaces exposed to marine environments in order to reduce biofouling of surfaces immersed in the marine environment. The present invention provides a method of fabricating block copolymer films using morphology trapping by lower temperature treatment in conjunction with solvent and or temperature annealing. The present invention inhibits the attachment of marine organisms, but it does not kill the organisms nor is it highly toxic. Cross-linked AB diblock or higher block copolymers, where A and B and any additional blocks if present have different hydrophobicity i.e. A is hydrophobic and B is hydrophilic, mixed with a photo-initiator films that preserve their nanosize domains when immersed in water. The block copolymer films inhibit settlement of marine organisms and can be used as marine antifouling coatings.

The invention relates to a cloning and function expression method of an adversity stress AhMYBL6 gene in peanut. The adversity stress AhMYBL6 gene is synthesized by RT-PCR (reverse transcription-polymerase chain reaction) cloning by the steps of preparation and treatment of materials, extraction of RNA (ribonucleic acid) and synthesis of cDNA (complementary deoxyribonucleic acid). The open reading frame of the gene is 861bp, and 287 amino acids are coded totally. The homology of the amino acid sequence of the gene with the MYb family proteins GmMYB4, GmMYB29 and GmMYB29A2 of the soybean is higher than 60%. The fluorescent quantitative PCR verifies the expression patterns of the AhMYBL6 under low temperature, salt stress and drought stress; the result indicates that the expression levels of the AhMYBL6 are obviously enhanced after various stress treatment for 1 hour, and kept at high levels all the time thereafter; and the expression level of the AhMYBL6 in the leaf subjected to low-temperature treatment is maximally enhanced by 20 times. After the gene is transferred into peanut by transgenic means, compared with the control, the transgenic plant has obvious characteristics of cold tolerance, salt tolerance and drought tolerance. The AhMYBL6 gene can obviously enhance the stress tolerance of the peanut.

The invention discloses bacteria capable of synchronously removing organic substances and ammonia nitrogen in water of a slightly polluted water source under low-temperature and aerobic conditions and a screening and domesticating method, which relate to bacteria and a screening and domesticating method and solve the problem that the prior bacteria are unsuitable for treating the slightly polluted water source as well as low temperature treatment. The bacteria SRA10 of the invention are acinetobacter lwoffi which belong to acinetobacter baumannii and are preserved in China General Microbiological Culture Collection Center on January 19th, 2009, with a preservation number of CGMCC No.2889. The screening method comprises the following steps: I, separating and purifying; II, preparing bacterial solution; III, screening and cultivating a bacterial solution; IV, screening and cultivating a bacterial solution with a live strain; V, repeating the step IV for 1 to 2 times, and stepwise domesticating the live strain; and VI, taking a bacterial solution of the domesticated strain, and rescreening the bacterial solution to obtain the bacteria. The bacteria obtained by screening and domesticating can process water of the slightly polluted water source under a condition of a low temperature of 2 to 10 DEG C.

The invention belongs to the technical field of textile printing and dyeing, and in particular relates to a dyeing and finishing technology for a cotton / viscose ammonia multi-fiber stretch fabric employing bio-enzyme low-temperature treatment. The dyeing and finishing technology comprises a pretreatment technology, a dyeing technology and a post-finishing technology. According to the printing and dyeing method for a cotton / viscose stretch fabric, room-temperature stacked desizing is carried out by a biologic desizing enzyme; and an efficient refining agent is selected, so that various pretreatment indexes are effectively improved; soft and fluffy hand feeling of the cotton / viscose stretch fabric is achieved; energy conservation and emission reduction are achieved through low-temperature desizing; potential drapes in the fabric processing process are eliminated; and smooth production of the dyeing procedure is facilitated. A light-colored fabric is dyed from pad dyeing, and a dark fabric is subjected to cold pad-batch dyeing, so that an improvement of various color fastness indexes is met; dyeing process parameters are optimized; and uniform and full dying material quality is obtained. A softening agent is preferably adopted in fabric post-finishing, so that the hydrophilicity of the fabric is achieved; and the wearing comfort of the fabric is achieved.

A microhollow cathode discharge assembly capable of generating a low temperature, atmospheric pressure plasma micro jet is disclosed. The microhollow assembly has at two electrodes: an anode and a cathode separated by a dielectric. A microhollow gas passage is disposed through the three layers, preferably in a taper such that the area at the anode is larger than the area at the cathode. When a potential is placed across the electrodes and a gas is directed through the gas passage into the anode and out the cathode, along the tapered direction, then a low temperature micro plasma jet can be created at atmospheric pressure. Selection of gas microhollow geometry and operational characteristics enable the application of the assembly to low temperature treatments, including the treatment of living tissue.

The invention relates to an improved ultrasonic-assisted pollen mediated plant genetic transformation method, and aims to obviously increase the setting percentage of the plant fertilized by pollen subjected to ultrasonic treatment, thereby increasing the number of transformants obtained for each treatment. The method comprises the following steps: by using an agrobacterium Ti plasmid carrying an exogenous gene segment, colibacillus plasmid or any other DNA vector as a genetic donor and using plant pollen as a receptor, mixing the pollen and exogenous DNA in a 5-50% sucrose solution subjected to aeration and low-temperature treatment, and transferring the exogenous gene into the receptor pollen under the assisting action of ultrasonic; fertilizing the treated pollen onto the stigma of the plant, and harvesting when the grains become ripe; and in the subsequent growth season, sowing the harvested seeds which are obtained after the fertilization of the transformed pollen, screening the germinated seeds and seedlings, carrying out PCR (Polymerase Chain Reaction) amplification and Southern hybridization on the DNA of a seedling sample, and further determining the transformant. The method provided by the invention does not need tissue culture, does not have species or genotype dependency, and can shorten the genetic transformation breeding time and save the manpower and material resources.