Product
Patsnap Eureka
For R&D, Patsnap Eureka makes reading and utilizing patents & technical documents easy.
Patsnap Eureka AIR
Designed for self-driven R&D workflows. Generate viable solutions, solve complex R&D challenges, empower your innovation with AI.
Patsnap Eureka Materials
Designed for material experts only. Revolutionize your material R&D, from search, analyze, to developing new materials.
Feature
TechResearch
Generate reliable direction feasibility study reports for your R&D in just a few steps.
TechSeek
Discover and master advanced knowledge NOW. Basics, ideas, possibilities, all at once.
TechMind
As an expert in R&D Theories, TechMind can generates customized viable solutions instantly.
TechRisk
Analyze your overall solution with one click, know your potential R&D risks in advance.
TechMonitor
Get weekly tech updates, stay abreast of the latest tech innovations and key insights.
Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
73 results about "Phytohemagglutinins" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Mucoproteins isolated from the kidney bean (Phaseolus vulgaris); some of them are mitogenic to lymphocytes, others agglutinate all or certain types of erythrocytes or lymphocytes. They are used mainly in the study of immune mechanisms and in cell culture.
CIK cell, as well as preparation method and cell preparation thereof
InactiveCN101519646AIncrease the number of proliferationPromote cell proliferationMammal material medical ingredientsTissue cultureAbnormal tissue growthPhytohemagglutinins
The invention discloses a CIK cell, as well as a preparation method and a cell preparation thereof. The method for preparing the CIK cell comprises the following steps: placing a separated mononuclear cell in a culture fluid containing phytohemagglutinin; transplanting the mononuclear cell into a culture flask enveloped by antiCD3 monoclonal antibody and antiCD28 monoclonal antibody after the cell is cultured for 24 to 72 hours; and adding a culture fluid containing IL-1alpha and IL-2 into the culture flask to keep on culturing for 5 to 15 days, and separating the cells into different flasks to be cultured every 2 to 3 days. The CIK cell prepared by the method has the characteristics of obvious improved cell proliferation, great increase of CD8 cell proportion, wide antineoplastic spectrum and strengthened antineoplastic activity. The CIK cell and the cell preparation can effectively prevent the metastasis and the recrudescence for of postoperative patients with tumor, can be combined with chemicotherapy to effectively reduce toxic and side effects of the chemicotherapy, strengthens the survivability tolerance of the patients to improve healing efficacy, and can obviously prolong lifecycle to of end-stage patients so as to improve the life living quality.
Owner:上海德嘉生物科技有限公司 +1
Extract method for lectin of leguminous plants
A method for extracting bean phytohemagglutinin belongs to the extraction technical field of useful constituents of plants. The method is a water extraction method in which the extraction is performed at 40-60 DEG C based on the following steps: crushing bean seeds to 40-100 meshes, adding soak solution to extract bean husk at the temperature of 40-60 DEG C for 60-180min and then fish the bean husk, performing rough separation to remove bean dregs, centrifugally separating to obtain supernatant with the bean phytohemagglutinin, further performing separation and purification on the supernatant, condensing or drying the supernatant to obtain the phytohemagglutinin. Ultrasonic wave or microwave can be exerted in the whole or part of the extraction process, or the ultrasonic wave and the microwave are alternately exerted to perform auxiliary extraction. A traditional low-temperature soaking and low-temperature treatment method is completely discarded in the method, and practice proves that the method is a simple and efficient method for extracting the phytohemagglutinin in short time. Related detection proves that the obtained phytohemagglutinin can meet conventional experimental needs and medicinal needs. The method helps overcome the disadvantage of more chemical reagent composition residue and long extraction time of the products obtained by the prior art.
Owner:YUNNAN KANGZHOU BIOLOGICAL SCI & TECH
Preparation method of high-purity, high-multiplication capacity and high-cytotoxin activity CIK (cytokine induced kill) cell
ActiveCN102154206AIncrease the number ofHigh activityMammal material medical ingredientsBlood/immune system cellsPhytohemagglutininsPeripheral blood mononuclear cell
The invention belongs to the technical field of cell culture in vitro, and particularly relates to a preparation method of high-purity, high-multiplication capacity and high-cytotoxin activity CIK (cytokine induced kill) cell. The method comprises the following steps: collecting and separating peripheral blood mononuclear cell of a patient, eliminating CD4+CD25+Treg cell by means of Mini MACS (magnetic active cell sorting) method, and sorting to obtain CD3+, CD4+ and CD8+T cells; and putting the obtained cells into culture solution containing phytohemagglutinin (PHA), so that the PHA concentration in the suspension liquid is 100ng / ml, hatching for 24h under the culture condition of 5% CO2 at 37 DEG C, transferring the hatched suspension liquid into a cell culture bottle coated by CD3 monoclonal antibody (1mug / ml), adding IFN (interferon)-gamma (1000U / ml), adding IL (interleukin)-2(500U / ml) and IL (interleukin)-21(1000U / ml) after 48h, compensating sodium selenite-containing (0.005mg / L)cell culture after four days, and continuously culturing for 7-14 days to obtain the high-purity, high-multiplication capacity and high-cytotoxin activity CIK (cytokine induced kill) cell. The quantity, the activity and the purity of the CIK cell which is prepared by the method and amplified in vitro are improved, so that the antineoplastic function of the CIK is enhanced.
Owner:郑骏年
Irx-2 modified manufacturing process
ActiveUS20090258395A1Peptide/protein ingredientsElectrical/wave energy microorganism treatmentPhytohemagglutininsWhite blood cell
A highly efficient method of making a primary cell derived biologic by purifying mononuclear cells (MNCs) in a automated cell processor to remove contaminating cells by loading leukocytes onto lymphocyte separation medium (LSM) and centrifuging the medium to obtain purified MNCs, storing the MNCs overnight in a closed sterile bag system, stimulating an induction mixture of the MNCs with phytohemagglutinin (PHA) or other mitogen and ciprofloxacin in a scalable cell culture device and producing a primary cell derived biologic from the MNCs, removing the mitogen from the induction mixture by filtering, incubating the induction mixture, clarifying the induction mixture by filtering to obtain a primary cell derived biologic supernatant, and clearing the primary cell derived biologic supernatant from adventitious agents by anion exchange chromatography, filtration. A closed system prevents contamination of the resulting primary cell derived biologic. An automated method of purifying cells. A method of scalably inducing cells.
Owner:BROOKLYN IMMUNOTHERAPEUTICS LLC
Method for separating red corpuscle from whole blood
InactiveCN101469323AFaster magnetic separationFast separationBlood/immune system cellsElectrical/wave energy microorganism treatmentPhytohemagglutininsMicrosphere
The invention relates to a novel method for quickly separating erythrocyte in whole blood of human beings, in particular to a method for separating the erythrocyte in the whole blood of the human beings by using polymer microsphers which are pre-coated by phytohemagglutinin or anti-erythrocyte antibodies and contain magnetic materials. The method can quickly and highly efficiently separate the erythrocyte from a whole-blood sample of the human beings.
Owner:INTEC PROD INC
A kind of compound preparation for human body and application thereof
InactiveCN102274486AHigh activityEffective fertilizationCosmetic preparationsPeptide/protein ingredientsHuman usePhytohemagglutinins
A compound preparation for human external use and its application belong to the technical field of human preparations. The lectin and the white fungus polysaccharide are used as active components, and the relative weight ratio between the two active components contained in the preparation is: 0.5-1.0 parts of the lectin and 1.5-3.0 parts of the white fungus polysaccharide. Said lectin is preferably kidney bean lectin. The dosage form is preferably a gel solution. 100ml of the gel solution contains 0.5-1.0 grams of plant lectin, 1.5-3.0 grams of tremella polysaccharide, 0.8-0.9 grams of NaCl, and pH buffers, stabilizers, and preservatives. The preparation is mainly used for external use by humans during sexual behavior, and it also has the functions of contraception, prevention and treatment of sexually transmitted diseases and lubrication; it can also be used as skin care products. Its principle of action is brand new and will not affect the human endocrine system; it can interfere and prevent HIV and other pathogens from invading normal cells in the body; it is extremely safe.
Owner:YUNNAN NORMAL UNIV
IRX-2 modified manufacturing process
ActiveUS8470562B2Peptide/protein ingredientsElectrical/wave energy microorganism treatmentPhytohemagglutininsWhite blood cell
A highly efficient method of making a primary cell derived biologic by purifying mononuclear cells (MNCs) in a automated cell processor to remove contaminating cells by loading leukocytes onto lymphocyte separation medium (LSM) and centrifuging the medium to obtain purified MNCs, storing the MNCs overnight in a closed sterile bag system, stimulating an induction mixture of the MNCs with phytohemagglutinin (PHA) or other mitogen and ciprofloxacin in a scalable cell culture device and producing a primary cell derived biologic from the MNCs, removing the mitogen from the induction mixture by filtering, incubating the induction mixture, clarifying the induction mixture by filtering to obtain a primary cell derived biologic supernatant, and clearing the primary cell derived biologic supernatant from adventitious agents by anion exchange chromatography, filtration. A closed system prevents contamination of the resulting primary cell derived biologic. An automated method of purifying cells. A method of scalably inducing cells.
Owner:BROOKLYN IMMUNOTHERAPEUTICS LLC
Engineering bacterium, construction method and application for producing immobilized alkaline pectinase nano micro-spheres
ActiveCN105296409ARealize fixed productionAvoid cumbersome proceduresBacteriaMicroorganism based processesEscherichia coliPectinase
Owner:XI AN JIAOTONG UNIV +1
Serum-free human peripheral blood lymphocyte culture medium
The invention relates to a biological agent and particularly relates to a lymphocyte culture medium. The lymphocyte culture medium comprises lectin, a basal culture medium and a serum substitution, wherein the lectin is L-type phytohemagglutinin; the basal culture medium is an RPMI1640 basal culture medium; and the serum substitution comprises bovine serum albumin, recombinant human insulin, ferric citrate and an amino acid mother solution. By adopting the serum-free human peripheral blood lymphocyte culture medium, the animal or human serum is not used so that the cost of the culture medium is reduced and the disease dissemination risk is also reduced. The components of the serum-free human peripheral blood lymphocyte culture medium are determined so that the problems of complex traditional serum components, quality differences in different batches and fluctuated culture effect can be avoided. The culture medium provided by the invention is high in lymphocyte conversion rate and lymphocyte metaphase index, low in reagent cost and stable in quality, and the cultured cell can be used for clinical diagnosis of the karyotype analysis.
Owner:广州和能生物科技有限公司
Induced culture composition capable of promoting proliferation of spleen-derived CD8 positive T cell and application of induced culture composition
The invention relates to an induced culture composition capable of promoting proliferation of a spleen-derived CD8 positive T cell and application of the induced culture composition. The induced culture composition comprises a culture medium and inducing factors added in the culture medium, the inducing factors comprises phytohemagglutinin, a CD3 antibody and a CD28 antibody, the final concentration of the phytohemagglutinin is 1 mu g / mL to 10 mu g / mL, the final concentration of the CD3 antibody is 1 mu g / mL to 10 mu g / mL, the final concentration of the CD28 antibody is 1 mu g / mL to 10 mu g / mL. The induced culture composition provided by the invention can promote proliferation of the spleen-derived CD8 positive T cell.
Owner:深圳灵赋拓普生物科技有限公司
Method for enhancing reproductive capacity of CIK (Cytokine Induced Killer) cell and improving tumor cell killing capacity
InactiveCN102978159AHigh mitogenic effectStrong cytotoxicityBlood/immune system cellsPhytohemagglutininsTreatment effect
The invention provides a method for enhancing the reproductive capacity of CIK (Cytokine Induced Killer) cells and improving tumor cell killing capacity. The method comprises the following steps of: sterilely collecting and separating the peripheral blood mononuclear cells of a healthy person; carrying out resuspension on the obtained peripheral blood mononuclear cells by using a Q007 culture medium; adding 24-26 ug / ml of PHA-L type phytohemagglutinin; and culturing for at least 15 days in the Q007 culture medium. The method provided by the invention has the beneficial effects that the reproductive capacity of the CIK cells and the cytotoxic effect are enhanced by adding 24-26 ug / ml of the PHA-L type phytohemagglutinin in a CIK cell culturing process, thereby enhancing the kill rate and the cure effect of the tumor cells.
Owner:深圳市中美康士生物科技有限公司
Chromosome preparation method, as well as required culture medium and preparation method thereof
InactiveCN102443623AQuality improvementEasy to check and analyzeMicrobiological testing/measurementPhytohemagglutininsPrenatal diagnosis
The invention discloses a chromosome preparation method, as well as a required culture medium and a preparation method thereof, and is used for solving karyotype analysis problem of chromosome. The culture medium consists of RPMI (Roswell Park Memorial Institute) 1640, heparin sodium, HEPES (2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid), L-glutamine, NaHCO3, benzylpenicillin potassium, streptomycin sulphate, bovine serum and phytohemagglutinin (PHA). The detection method comprises the following steps: implanting 0.3 to 0.4ml of human peripheral blood into the culture medium; adding colchicinamide in 2-4 hours before culture is terminated to realize that the cell is terminated in anaphase; culturing the cell after 68 to 72 hours to harvest the cell; performing hypotonicity for 40 minutes, three times of fixation, banding, dyeing and other treatments; and performing chromosome analysis under a microscope to determine whether the peripheral blood supplier has a phenomenon of chromosome abnormality. The culture medium disclosed by the invention has convenience for use, simpleness in operation, low cost and low patient detection fee, and is suitable for genetic diagnosis, infertility and prenatal diagnosis in each level of hospitals.
Owner:苏州苏大赛尔免疫生物技术有限公司
Method for making soybean-containing nutritious steamed bread
The invention discloses a method for making soybean-containing nutritious steamed bread. The method comprises the following steps: taking a raw material, boiling soybeans, grinding, kneading dough, fermenting, molding, proofing and steaming. The steamed bread obtained by raw soybean milk or raw soybean flour and cereal fermentation has a very strong soybean flavor, while the soybean phytohemagglutinin also affects the yeast fermentation process, so that the steamed bread is not easily accepted due to poor sensory quality. Therefore, the beany flavor and soybean phytohemagglutinin are ruined after the soybeans are boiled, so that the yeast normally ferments; the boiled soybeans are treated by a colloid mill to reach the micron level, so that the taste of the steamed bread cannot be affected, the soybean flavor of the streamed bread is enhanced, and the sensory level and nutritional value of the steamed bread are improved. The soybean component of the soybean-containing nutritious steamed bread can reach 30 to 50 grams, meeting the nutritional requirements of resident dietary guidelines in China; and if eaten for a long term, the steamed bread has a greater role in promoting the health of all residents.
Owner:CANGZHOU MEDICAL COLLEGE
Preparation method of chromosomes of adult epinephelus akaara
InactiveCN104483178AIncrease injection concentrationGood effectPreparing sample for investigationPhytohemagglutininsEpinephelus akaara
The invention discloses a preparation method of chromosomes of adult epinephelus akaara and belongs to the field of cell biology. The preparation method comprises the following steps: pretreatment of materials, preparation of kidney cell suspension, low-permeation treatment, fixation, dropping and dyeing, wherein the pretreatment of the materials comprises the steps of with an in vivo injection method of phytohemagglutinin (PHA), injecting PHA into the pectoral-fin base part of the experimental adult epinephelus akaara with 15 micrograms in each gram of the adult epinephelus akaara; after 24 hours, injecting colchicine solution with 2 micrograms in each gram of the adult epinephelus akaara; and after 2.5-3 hours, taking out the head kidney. The preparation method disclosed by the invention has the advantages that the method for one-time injection of PHA is adopted, simultaneously, the injection concentration is increased, and good effect can be obtained after 24 hours, so that the time is saved; and due to the characteristics of tissue cells of the epinephelus akaara, complete cells are not easily obtained when the low-permeation treatment is carried out in the prior art, so that the prior art is creatively changed and a clear chromosome image is obtained.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI +2
Method for extracorporeally preparing transfer factor against specificity of infectious bronchitis virus of chickens
InactiveCN101757027AImprove immunityAntiviralsMammal material medical ingredientsPhytohemagglutininsLymphocytes.immature
The invention relates to a method for extracorporeally preparing a transfer factor against the specificity of infectious bronchitis viruses of chickens. In the invention, splenic organs or peripheral blood of chickens are used as raw materials to make a lymphocyte monolayer, and then phytohemagglutinin and infectious bronchitis viruses of chickens are used to culture lymphocytes through induction so as to extracorporeally produce a large quantity of transfer factors against the specificity of the infectious bronchitis viruses of chickens. The anti-specificity transfer factor prepared by using the method of the invention is used for treating infectious bronchitis of chickens and can raise the immunity of poultry.
Owner:TIANJIN SHENGJI GRP CO LTD
Method for synthesizing PHA (Phytohemagglutinin) by using pseudomonas putida KT2442
InactiveCN103215318AEnvironmentally friendlyEasy to adjustMicroorganism based processesFermentationPhytohemagglutininsPseudomonas putida
The invention provides a method for synthesizing PHA (Phytohemagglutinin) by using pseudomonas putida KT2442. The method comprises the following steps of: 1, mixing a small quantity of pseudomonas putida KT2442 in an LB (lysogeny broth) culture medium, and culturing to obtain a seed culture solution containing strains; and 2, adding the seed culture solution obtained in the step 1 and a carbon source to the culture medium, culturing, separating, quick-freezing, drying, and refining to obtain a final product PHA. According to the method, the pseudomonas putida KT2442 in culture wastewater is used, the supply of the carbon source is adjusted, and correlated matrix octane acid and non-correlated matrix glucose are respectively added in the culturing process, thus the final product PHA is obtained. The product obtained by using the method has strong decomposability, low glass transition temperature, good flexibility and adhesion and can be applied to the medicine field, and therefore, the production has a bright application prospect.
Owner:SHANGHAI MEDICAL INSTR COLLEGE
Method for rapidly preparing prematurely condensed chromosomes of human peripheral blood lymphocytes
InactiveCN103805564AReduce incubation timeShort timeBlood/immune system cellsPhytohemagglutininsColchicine
The invention relates to a method for rapidly preparing prematurely condensed chromosomes of human peripheral blood lymphocytes. The method mainly comprises the following steps: 1, processing a blood sample by phytohemagglutinin, allowing the processed blood sample to stand in a constant temperature incubator with the temperature of 37DEG C for 45-90min; 2, culturing lymphocytes in the phytohemagglutinin processed blood sample in a mixed culturing liquid containing colchicine, ATP, 5% fetal calf serum, CalyculinA and CDK1 / CyclinB at 37DEG C for 3-12h; 3, carrying out hypoosmotic treatment of the cultured lymphocytes by using 0.075mol of KCl for 10-20min; and 4, adding an immobile liquid into a hypoosmotic liquid containing the hypoosmotic lymphocytes to realize first-time immobilization, adding the immobile liquid to realize second-time immobilization, and suspending the obtained lymphocytes in the immobile liquid, wherein each of the first-time immobilization and the second-time immobilization is carried out for 10-15min. The preparation method provided by the invention takes a substantially shorter time of 3-12h than routine preparation methods of the prematurely condensed chromosomes.
Owner:NAT INST FOR RADIOLOGICAL PROTECTION & NUCLEAR SAFETY CHINESE CENT FOR DISEASE CONTROL & PREVENTION
Lymphocyte serum-free medium with stable pH value as well as preparation method and application of lymphocyte serum-free medium
ActiveCN105039251ASpeed up cloningIncrease in the number of mitotic cellsBlood/immune system cellsHemagglutininSerum free media
The invention provides a lymphocyte serum-free medium with a stable pH value. The lymphocyte serum-free medium comprises the following components: a basic medium, 10 mg / L of transferrin, 0.01 mg / L of sodium selenite, 110 mg / L of pyruvic acid, 1.5 mg / L of ethanolamine, 10 mg / L of insulin, 10-30 mg / L of a glutamine substitute, 300 mg / L of lipid-type bovine serum albumin, 0.001 mg / L of copper sulfate, 0.0005 mg / L of ammonium metavanadate, 0.00005 mg / L of manganese chloride, 0.8 mg / L of zinc sulfate, 2 mg / L of vitamin E, 100 mg / L of phytohemagglutinin (PHA) and 10-50 mM of a pH buffer system. The invention further discloses a preparation method and application of the lymphocyte serum-free medium. Through the adoption of the lymphocyte serum-free medium, lymphocyte differentiation can be improved, and a large number of division-phase lymphocytes are generated; moreover, the pH value is stable , so as to facilitate stable storage of the lymphocyte serum-free medium.
Owner:广州达晖生物技术股份有限公司
Method for extracorporeally preparing transfer factor against specificity of bursal disease virus of chickens
InactiveCN101759765AImprove immunityChicken bursal virusPeptide preparation methodsCytokines/lymphokines/interferonsPhytohemagglutininsTransfer factor
The invention relates to a method for extracorporeally preparing a transfer factor against the specificity of bursal disease viruses of chickens. In the invention, splenic organs or peripheral blood of chickens are used as raw materials to make a lymphocyte monolayer, lymphocytes are extracorporeally cultured, and then phytohemagglutinin and bursal disease viruses of chickens are used to culture the lymphocytes through induction so as to extracorporeally produce a large quantity of transfer factors against the specificity of the bursal disease viruses of chickens. The transfer factor against the specificity of bursal disease viruses of chickens extracorporeally prepared by using the method of the invention is used for treating infectious bursal diseases of chickens and can raise the immunity of poultry.
Owner:TIANJIN SHENGJI GRP CO LTD
Use of hAMSCs (Human Amniotic Mesenchymal Stem Cells) in preparing drug for treating acute graft-versus-host disease
InactiveCN109674818ADecrease in secretionImprove proliferative abilityUnknown materialsImmunological disordersPhytohemagglutininsPeripheral blood mononuclear cell
The invention provides use of hAMSCs (Human Amniotic Mesenchymal Stem Cells) in preparing a drug for treating the acute graft-versus-host disease. The hAMSCs can meet basic biological characteristicsof mesenchymal stem cells of adult tissues, also expresses an embryonic stem cell marker, and has multiplication capacity stronger than that of BMSCs (Bone Marrow Mesenchymal Stem Cells). When the hAMSCs and PHA (phytohemagglutinin) activated healthy human PBMCs (Peripheral Blood Mononuclear Cells) are co-cultured, the proportion of Th1 and Tc1 cell subsets is reduced, the proportion of Th2, Tc2 and Treg cell subsets is increased, the secretion level of IL-2 and IFN-gamma is reduced, the IL-10 level is increased, and therefore, the hAMSCs can be used for preparing the drug for treating the acute graft-versus-host disease.
Owner:NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
Method for quantitatively detecting coagulant activity of bean phytohemagglutinin
ActiveCN103558176AThe detection method is simpleThe results are relatively consistentPreparing sample for investigationColor/spectral properties measurementsPhytohemagglutininsActivity concentration
The invention discloses a method for quantitatively detecting the coagulant activity of bean phytohemagglutinin and belongs to the technical field of plant component detection. According to the method, a red cell suspension for detection is subjected to cell quantification; meanwhile, a coagulant activity concentration standard curve is drawn by using the bean phytohemagglutinin with the known concentration as a standard substance, and the coagulant activity concentration of the bean phytohemagglutinin is further calculated, so that the coagulant activity of the bean phytohemagglutinin is quantified.
Owner:JIANGHAN UNIVERSITY
Recombinant strain of mink IFN-Gamma gene
InactiveCN101531986AHas antiviral activityBacteriaMicroorganism based processesEscherichia coliBiotechnology
The invention relates to a recombinant strain of a mink IFN-Gamma gene, which is characterized by being an IFN-Gamma gene sequence which is cloned from peripheral mink bloodlymph cells (PMBC) simulated by phytohemagglutinin (PHA) and being a recombinant plasmid vector containing a nucleotide sequence; i.e., using a plasmid vector pGM-T which is formed by the cutting of a cloning vector by taking an EcoR V enzyme as the cutting point and then adding a 'T' from the '3' end at both sides; and TA cloning is carried out on the nucleotide sequence of a coding Gamma-interferon which is amplified from the peripheral mink bloodlymph cells so as to construct the recombinant plasmid of the nucleotide sequence of the mink Gamma-interferon. In addition, Escherichia coli conversed from the plasmid vector is used as a host. The recombinant strain aims at laying down foundation for researching and developing genetically engineered mink recombinant interferons biological products with anti-viral activity and immunomodulatory activity.
Owner:INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
Method for taking glucose as substrate to continuously produce polyhydroxylbutyrate valerate (PHBV) in one step by using halophilic mixed bacteria
InactiveCN103865960AEasy to operateReduce manufacturing costFermentationPhytohemagglutininsDecomposition
The invention discloses a method for taking glucose as a substrate to continuously produce polyhydroxylbutyrate valerate (PHBV) in one step by using halophilic mixed bacteria, and belongs to the technical field of PHBV production. The method comprises the following steps: collecting bottom mud at an estuary to inoculate into a fermentation tank, and then operating the fermentation tank by adopting an intermittent operation mode; injecting air into the fermentation tank at a constant speed, controlling the dissolved oxygen concentration in a substrate solution at 1-3mg / L, and continuously running for 5-8 hours, wherein the operation conditions of the fermentation tank are as follows: the constant temperature of the fermentation tank is 37+ / -0.1 DEG C, and the pH is 6-8, and discharging 75% mixed solution in the fermentation tank, wherein the mass percent of the PHBV can be up to over 30% by continuous repetition; recovering biomass in the discharged mixed solution, and extracting phytohemagglutinin (PHA). The method is simple to achieve, and engineering operation is easy to carry out, and the produced PHA is a PHBV copolymer, and has high decomposition temperature and low melting temperature. By adopting the method, the production cost of the PHA is reduced by 20-30%.
Owner:BEIJING UNIV OF TECH
Bacteriostatic and antiviral preparation taking kidney bean phytohemagglutinin as major ingredient
ActiveCN108404111ALower pHRestore self-cleaning abilityAntibacterial agentsAntimycoticsPhytohemagglutininsAdditive ingredient
The invention discloses a bacteriostatic and antiviral preparation taking kidney bean phytohemagglutinin as a major ingredient, and belongs to the technical field of medicines. The bacteriostatic andantiviral preparation consists of the following active components according to relative parts by weight: 0.1-1.0 part of the kidney bean phytohemagglutinin, 0.2-2.0 parts of one or two of stachyose and raffinose as well as one or more of the following active components: 0.1-0.5 part of povidone iodine, 0.1-0.3 part of polyhexylene biguanidine and 0.1-0.5 part of chlorhexidine; and in addition, a thickening agent, which is hydroxyethyl cellulose, is required. The bacteriostatic and antiviral preparation provided by the invention is concrete in components, and is capable of achieving quantitative control and guaranteeing the consistency and stability of overall quality; a concrete inhibitory effect can be achieved on bacteria, fungi and viruses; therefore, the bacteriostatic and antiviral preparation is relatively broad in antibacterial and antiviral ranges; and the product (the bacteriostatic and antiviral preparation), which is required to be preserved at low temperature, is long in preservation duration and convenient to use.
Owner:YUNNAN KANGZHOU BIOLOGICAL SCI & TECH
Method of extracting phytohemagglutinin
InactiveCN110283242AHigh purityHigh activityPeptide preparation methodsPlant peptidesPhytohemagglutininsHemolysis
The invention relates to a method of extracting phytohemagglutinin and belongs to the technical field of phytohemagglutinin extraction. The phytohemagglutinin can be extracted from beans by soaking in a buffer solution, acid conditioning to settle protein and adding 0.1-0.4% of sodium chloride solution to settle the protein; the extraction method is simple and convenient; and a reagent used in an extraction process is cheap, easy to obtain and harmless to a human body. Extracted PHA (phytohemagglutinin) protein is high in purity and good in activity; and when the extracted PHA protein is applied to a lymphocyte culture medium, blood coagulation or hemolysis can be avoided, and a good effect of stimulating lymphocyte growth is achieved. The extraction method is suitable for large-scale production of the PHA protein.
Owner:河南赛诺特生物技术有限公司
Pet edible pea pods and preparation method thereof
InactiveCN106359857AHigh medicinal valueHigh nutritional valueAnimal feeding stuffAccessory food factorsPhytohemagglutininsVitamin C
The invention belongs to the field of pet foods and especially relates to pet edible pea pods and a preparation method thereof. Peas have high medicinal and nutritional values and are rich in the vitamin C and enzymes being capable for decomposing nitrosamine in body, thereby decomposing the nitrosamine, and also contain abscisic acid, gibberellins and phytohemagglutinin and the like substances, which have anti-bacterial and anti-inflammation effect and metabolism improving function. The Peas also contains abundant dietary fibers that can prevent constipation of pets and has a function of relaxing bowels. Fish skin contains abundant proteins and various micro-elements, wherein the proteins mainly are high-molecular collagen and mucopolysaccharides. Chicken meat is delicious and is easy to digest. In the invention, the pea pod product is prepared from the peas, the fish skin and the chicken meat. The pea pod product has novel and attractive appearance and is more attractive to pet dogs, thereby improving appetite. The peas, the fish skin and the chicken meat are organically combined, thereby improving the nutritional value of the pea pod pet food.
Owner:YANTAI CHINA PET FOODS GRP
Lymphocyte culture medium with stable pH value and preparation method and application thereof
ActiveCN104988120AStable pHFor long-term storageBlood/immune system cellsHemagglutininPhytohemagglutinins
The invention provides a culture medium with a stable pH value. The culture medium is composed of a 1640 base culture medium, a 100 mg / L phytohemagglutinin (PHA), a calf serum with the volume percentage of 20% and a 5-50 mM sodium glycerophosphate / 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) dual-buffering system. The sodium glycerophosphate / HEPES dual-buffering system is composed of a phosphate buffer solution, sodium glycerophosphate and 4-HEPES, wherein the phosphate buffer solution contains Na2HPO4 and KH2PO4. The invention also provides a preparation method of the culture medium and an application of the culture medium in lymphocyte culture. The pH value of the culture medium is not affected by gas composition in the environment, and the pH value during the lymphocyte culture also can be stabilized; and long-term preservation of the culture medium is facilitated.
Owner:广州达晖生物技术股份有限公司
Preparation method of enhanced CIK (Cytokine Induced Killer) cell, and cell preparation
ActiveCN103937741AHigh cytotoxic activityIncrease the value-added multipleMammal material medical ingredientsBlood/immune system cellsPhytohemagglutininsPeripheral blood mononuclear cell
The invention discloses a preparation method of an enhanced CIK cell, and a cell preparation. The method comprises the following steps: separating to obtain a peripheral blood mononuclear cell from peripheral blood collected from a sufferer; putting the peripheral blood mononuclear cells into a blood-free medium to culture, so as to obtain karyocyte of which the concentration is (1-3)*10<6> / ml; adding IFN (interferon)-gamma, phytohemagglutinin and PGE2 (Phenyl Glycidyl Ether), transferring to a culture bottle or a culture bag to culture for 24 hours, and then adding a CD3 monoclonal antibody, IL-2 (interleukin-2), IL-1alpha, IL-4 and GM-CSF (granulocyte-macrophage colony-stimulating factor); continuing to culture for 3-5 hours, and then controlling the cell density at (1-6)*10<6> / ml; and centrifugally colleting to obtain the enhanced CIK cell after continuously culturing for the 14th to 21st days. The enhanced CIK cell preparation comprises the enhanced CIK cell, human serum albumin, IL-2, amino acids, vitamins and inorganic salts. Compared with a preparation in the patient, the CIK preparation has the advantages that the preservation time of the CIK preparation is obviously prolonged, and is prolonged to 36 hours from 6 hours at room temperature. The CIK preparation is higher in reliability in clinical application, and the safety is effectively ensured.
Owner:武汉光谷高新科技发展有限公司
In-vitro preparation method of transfer factors capable of resisting duck viral hepatitis
InactiveCN102697808AFree from infectionAvoid carryingPeptide/protein ingredientsMammal material medical ingredientsDuck hepatitis A virusPhytohemagglutinins
The invention relates to an in-vitro preparation method of transfer factors capable of resisting duck viral hepatitis. In the method, the transfer factors capable of resisting the duck viral hepatitis are prepared through the induction of duck hepatitis virus by utilizing an in-vitro lymphocyte cell culture method; and chicken spleen or peripheral blood is used as a basic raw material to prepare single-layer lymphocyte cells, and then the single-layer lymphocyte cells are induced and cultured through phytohemagglutinin and the duck hepatitis virus, so that a large number of specific transfer factors capable of resisting the duck hepatitis virus are generated in vitro.. The transfer factors can effectively inhibit the virus of duck viral hepatitis, so that the normal bodies cannot be infected with the duck viral hepatitis, and the radical pre-prevention action can be realized. The method provided by the invention has the characteristics of being simple and easy to operate and the like.
Owner:河南后羿生物工程股份有限公司
Method for extracting L-type and E-type phytohemagglutinins (PHAs)
PendingCN112707958AHigh purityEasy to operatePeptide preparation methodsPlant peptidesBiotechnologyPhytohemagglutinins
The invention relates to the technical field of plant extraction, and discloses a method for extracting L-type and E-type phytohemagglutinins (PHAs). According to the method, crude protein extraction is carried out through the steps of soaking, crushing, centrifuging, ammonium sulfate fractional precipitation and the like on beans, and then L-type and E-type PHAs are separated and purified through hydrophobic chromatography and ion exchange chromatography. The method is simple to operate, and the extracted L-type and E-type PHAs are high in purity and suitable for large-scale production of the L-type and E-type PHAs.
Owner:ZHENGZHOU IMMUNO BIOTECH
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com