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Method for separating red corpuscle from whole blood

A technology of red blood cells and anti-red blood cell antibodies, which is applied in the field of separation of red blood cells, can solve the problems of complex operation and long time consumption, and achieve the effect of meeting different applications and needs, accelerating the speed of magnetic separation, and facilitating popularization and application

Inactive Publication Date: 2009-07-01
INTEC PROD INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have their own characteristics, but they all take a long time and the operation is more complicated.

Method used

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  • Method for separating red corpuscle from whole blood

Examples

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Effect test

Embodiment 1

[0025] Example 1: Method for Separating Red Blood Cells Using Phytohemagglutinin Coated Magnetic Particles

[0026] Take 100g of red beans, wash them with pure water, soak them in 200ml of pure water for 24 hours, use a juicer to crush the red beans into soybean milk, add 50ml of pure water during the crushing process to improve the grinding efficiency, and centrifuge the milk at 6000g for 30 Minutes, the supernatant was retained and further purified by saturated ammonium sulfate method to obtain pure plant lectin for future use.

[0027] According to the ratio of 20ug phytolectin coated with magnetic polymer microspheres (average diameter 100nm) solution of 10% concentration per milliliter, the magnetic polystyrene microspheres were dissolved in PBS (50mM, pH7.4) buffer solution in advance (concentration 10%, m / v), adding the lectin under the condition of rapid stirring, stirring (100 rpm) at room temperature for 30 minutes. Then add BSA to a final concentration of 0.2%, sti...

Embodiment 2

[0030] Example 2: Method for Separating Red Blood Cells Using Anti-erythrocyte Antibody Coated Magnetic Particles

[0031] Take 1 mg of anti-erythrocyte antibody purchased from Beijing Xinchuang Bioengineering Co., Ltd. According to the ratio of 20ug anti-erythrocyte antibody coated with 10% concentration of magnetic polyethylene microspheres (average diameter 100nm) solution per milliliter, the magnetic polyethylene microspheres were dissolved in PBS buffer (50mM pH7.4) in advance (concentration 10%) , m / v), under the situation of rapid stirring, add anti-erythrocyte antibody, stir (100 rev / min) reaction at room temperature for 30 minutes, add BSA to final concentration 0.2%, stir (100 rev / min) reaction at room temperature for 15 minutes , finally centrifuged at 10000g for 20 minutes, discarded the supernatant, resuspended the pellet with PBS (50mM pH7.4) buffer solution, and obtained the magnetic polyethylene microsphere solution labeled with anti-erythrocyte antibody (the f...

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Abstract

The invention relates to a novel method for quickly separating erythrocyte in whole blood of human beings, in particular to a method for separating the erythrocyte in the whole blood of the human beings by using polymer microsphers which are pre-coated by phytohemagglutinin or anti-erythrocyte antibodies and contain magnetic materials. The method can quickly and highly efficiently separate the erythrocyte from a whole-blood sample of the human beings.

Description

field of invention [0001] The invention relates to a method for separating red blood cells from human whole blood, in particular to a method for separating red blood cells in human whole blood by using polymer microspheres containing magnetic materials pre-coated with lectin or anti-erythrocyte antibodies. The present invention can be widely used in clinical examination and medical scientific research and other fields where a large number of human whole blood samples are used. Background of the invention [0002] Blood samples are the most commonly used samples in clinical and scientific research, but they are generally limited to serum or plasma without red blood cells. Therefore, in many cases, whole blood samples need to be pretreated to separate red blood cells from them. There are many methods for separating red blood cells from whole blood in clinical and scientific research, including natural sedimentation, centrifugation, size exclusion filtration and adsorption filt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12N13/00C12N5/078
Inventor 王言帅
Owner INTEC PROD INC
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