34 results about "Erythrocyte antibody" patented technology

The invention relates to a method for detecting a plurality of antigenic molecules carried by erythrocytes and / or a plurality of anti-erythrocyte antibodies, said antigenic molecules carried by the erythrocytes consisting of antigenic molecules carried not only by the erythrocytes, but also by at least one other cell population, other than the blood group antigen molecules, said method comprising bringing a sample into contact with distinguishable beads, on which are attached a) antibodies specific for said antigens, or b) erythrocytes or erythrocyte membrane fragment.

The invention relates to a method for rapidly separating supernatant from human whole blood or suspended red blood cells by utilizing lyophilized preparation. The method comprises the following steps: the phytoagglutinin or the anti erythrocyte antibody is pre-coated on a magnetic polymer microsphere to be prepared into isolating reagent for the liquid whole blood or suspended red blood cells; the isolating reagent for the liquid whole blood or suspended red blood cells is absorbed by insoluble polymer, and after lyophylization, the isolating reagent is cut into thin slices to be prepared into isolating reagent for lyophilized whole blood or suspended red blood cells; and the isolating reagent for the lyophilized whole blood or suspended red blood cells is added to the human whole blood or suspended red blood cells to be uniformly mixed and then placed above a magnetic body, and the supernatant is rapidly separated. The lyophilized preparation preserved for long term at the normal temperature can be prepared by the method, and the supernatant can be rapidly separated form the human whole blood or suspended red blood cells under the conditions without ensuing the electric power and the centrifugal machine.

A process for the detection of antibodies in a test sample by preparing a suspension of erythrocytes with a test serum or plasma by mixing a test serum or plasma with erythrocytes; incubating the suspension of erythrocytes at a temperature of from 37° C. to 45° C. to bind any antibodies in the test serum or plasma to the surface of said erythrocytes; combining the suspension of erythrocytes with an amount of a solution of a macromolecule which is effective to agglutinate the erythrocytes; packing the resultant red cell agglutinates by centrifuging the suspension of erythrocytes; and, determining the presence of anti-erythrocyte antibodies by observing if antibody-dependent erythrocyte agglutination has occurred.

The invention relates to an SPA-antibody tripolymer, a cell treating kit containing the tripolymer, a preparation method and application thereof. The tripolymer comprises an anti erythrocyte antibody, an SPA and an antibody of a certain anti leukocyte antigen or other antigens. The tripolymer is combined with an erythrocyte through the anti erythrocyte antibody per se, the other antibody is combined with a corresponding leukocyte antigen (or other antigens), and then leukocyte (other cells, factors) and the erythrocyte are deposited through an erythrocyte sedimentation process or other methods of density gradient centrifugation and the like, thereby achieving the purpose of eliminating ingredients of corresponding cells and the like, connecting the erythrocyte with a corresponding antigen by the SPA, and being used as a mean for detecting a certain antigen. The invention has convenience and simpleness, and can be directly used for clinically separating and extracting stem cells / progenitor cells; and the collected and extracted stem cells / progenitor cells have no external markers. Meanwhile, the kit based on the tripolymer can be industrially produced so as to realize the popularization of the separation and purification technology of hematopoietic stem cells.