36 results about "Erythrocyte sedimentation" patented technology

The present invention provides methods and compositions for separating cells from a sample containing erythrocytes. The method is for recovering desired cells from a sample containing the desired cells, erythrocytes and undesired cells comprising: a) contacting the sample with a composition, said composition comprising: i) an erythrocytes aggregation reagent ii) at least one antigen recognizing moiety coupled to a magnetic particle, wherein said particle with said at least one antigen recognizing moiety specifically binds to at least one antigen specific for one or more undesired cellular components; b) applying simultaneously i) gravity sedimentation for sedimentation of erythrocytes and ii) a magnetic field gradient to said sample for immobilizing said magnetic particle generating a pellet and a supernatant phase, and c) recovering the desired cells from the supernatant phase. Compositions for the use within the present method are also disclosed.

The invention discloses a blood analyzing device (100) comprising a holder (110) arranged for carrying a container (10) having a cuvette (20) containing a blood sample (30). The container (10) is positioned in the holder (110) so that a longitudinal axis (60) of the cuvette (20) is angled relative a horizontal axis (70). A light source (120) provides light (40) into the sample (30) and a detector (130) detects the output light (50) from a sub-portion of the blood sample (30). Kinetic information indicative of the change in hemoglobin concentration in a measuring volume (32, 34) is determined by a Hb processor (145) from the detected output light (50). An ESR processor (140) determines the erythrocyte sedimentation rate of the sample (30) based on the kinetic information.

An apparatus and method for rapid determination of erythrocyte sedimentation rates for a blood specimen (29) which can be linearly transposed to Westergren sedimentation rates. The method includes the steps of inducing accelerated rouleaux formation in the specimen (29) in an amount sufficient to begin settling at substantially the decantation rate for the specimen. In one embodiment a structure (27) which produces a very thin cross-sectional region (37) of the specimen (29) inside the lumen (23) of a specimen container (21) is provided to accelerate rouleaux formation. In an alternative embodiment (120), accelerated rouleaux formation is accomplished using a centrifuge (122). A third embodiment employs a movable rod (223) mounted inside the specimen tube (221) to induce accelerated rouleaux formation. All embodiments of the process next employ gravity settling the specimen in a near horizontal oriented container (21, 121, 221). Thereafter, the amount of settling occurring is determined. A sealed specimen container (21, 121, 221) which permits thorough mixing of blood in a very small diameter container for use in performing the method also is provided.

A method for calibrating machines suitable to effect an analysis of a blood sample by means of measuring the erythrocyte sedimentation rate (ESR) and / or aggregation of the red corpuscles, wherein said measurement is effected by exploiting the optical density kinetics obtained from the measurement of the variation in the optical density of the blood sample in an interval of time, comprises a measuring step in which, by means of the same machine with which the measurement of the optical density is effected on said blood sample, a measurement is effected of the optical density of two latexes, or turbidimetric samples. Each of the two latexes has a known optical density, reproducible, measurable and different from each other. The method also comprises a comparison step, in which the difference is calculated between the values of optical density of the latexes as obtained from the measurement performed by the machine and the known values of optical density, to allow to determine at least one correction factor usable to calibrate said machine.

The invention discloses a blood detection micro-fluidic chip. The blood detection micro-fluidic chip comprises a substrate and a cover plate; and the cover plate and the substrate are in sealing fit to form a chip body; the chip body is provided with a plurality of separation and detection units, and the plurality of separation and detection units are radially distributed by taking the circle center of the chip body as an original point; each separation and detection unit comprises a whole blood sampling pool, a plasma separation pool, an erythrocyte sedimentation pool, a plasma storage pool, a quantification pool, a front primary waste liquid pool, a front secondary waste liquid pool, a rear primary waste liquid pool, a detection reagent sampling pool, a detection pool, a whole blood sample injection hole, a whole blood sample injection pool vent hole, a plasma storage pool vent hole, a rear primary waste liquid pool vent hole and a detection reagent sample injection hole, wherein the whole blood sampling pool, the plasma separation pool, the erythrocyte sedimentation pool, the plasma storage pool, the quantification pool, the front primary waste liquid pool, the front secondary waste liquid pool, the rear primary waste liquid pool, the detection reagent sampling pool and the detection pool are arranged on the substrate, and the whole blood sample injection hole, the whole blood sample injection pool vent hole, the plasma storage pool vent hole, the rear primary waste liquid pool vent hole and the detection reagent sample injection hole are formed in the cover plate. The blood detection micro-fluidic chip has separation and detection functions at the same time, can achieve plasma / serum separation, reagent injection, reaction and detection integration; and the problem that blood coagulation measurement and detection cannot meet the ever-increasing instant detection requirement is solved.

The invention relates to an SPA-antibody tripolymer, a cell treating kit containing the tripolymer, a preparation method and application thereof. The tripolymer comprises an anti erythrocyte antibody, an SPA and an antibody of a certain anti leukocyte antigen or other antigens. The tripolymer is combined with an erythrocyte through the anti erythrocyte antibody per se, the other antibody is combined with a corresponding leukocyte antigen (or other antigens), and then leukocyte (other cells, factors) and the erythrocyte are deposited through an erythrocyte sedimentation process or other methods of density gradient centrifugation and the like, thereby achieving the purpose of eliminating ingredients of corresponding cells and the like, connecting the erythrocyte with a corresponding antigen by the SPA, and being used as a mean for detecting a certain antigen. The invention has convenience and simpleness, and can be directly used for clinically separating and extracting stem cells / progenitor cells; and the collected and extracted stem cells / progenitor cells have no external markers. Meanwhile, the kit based on the tripolymer can be industrially produced so as to realize the popularization of the separation and purification technology of hematopoietic stem cells.

The embodiment of the invention discloses a method for purifying cord blood mononuclear cells. The method comprises: step 1, centrifuging human umbilical cord blood to obtain blood cells and plasma; step 2, subjecting the blood cells to erythrocyte sedimentation with hydroxyethyl starch to obtain a lymphocyte suspension; and step 3, performing gradient centrifugal separation on the lymphocyte suspension by using a Ficoll reagent so as to obtain the cord blood mononuclear cells. The method can effectively reduce the number of erythrocyte and thrombocyte in the final product, and the number of the purified cord blood mononuclear cells is large, and the purity and the motility rate are high.

The invention relates to a kit for high-purity high-recovery separation in vitro of allogeneic peripheral blood mononuclear cells and an application method of the kit. The allogeneic mononuclear cells separated by the kit can be used for immunotherapy of unexplained habitual abortion. The kit comprises a reagent I, namely a peripheral blood thinner, a reagent II, namely an erythrocyte sedimentation solution, a reagent III, namely mononuclear cell separating solution and a reagent IV, namely a mononuclear cell washing solution. The method for separating the allogeneic peripheral blood mononuclear cells by using the kit comprises the following steps: anticoagulation pre-centrifugation; erythrocyte initial reaction; erythrocyte sedimentation; two-step separation of the mononuclear cell separating solution; secondary purification of mononuclear cells; bacteria detection; mononuclear cell cryopreservation. According to the technology, the mononuclear cell recovery rate can achieve more than 90 percent, the purity can achieve more than 95 percent, the survival rate can achieve more than 98 percent, and the service efficiency of the peripheral blood mononuclear cells is greatly improved. Plasma is removed through the step of anticoagulation pre-centrifugation. Compared with the conventional separation technology, the dose of the separation reagent is reduced, and the separation cost is effectively reduced.

The invention discloses sample density separation liquid which comprises the following components in percentage by mass: 5-10% of glycerin, 3-5% of ethanol, 3-5% of sodium chloride, 79.8-88.9% of double distilled water and 0.1-0.2% of tea saponin. By adopting the sample density separation liquid, the cells of diagnostic value can naturally sedimentated more quickly while the erythrocyte sedimentation interference is reduced, thus a microscopic examination slice has clearer microscopic examination view and good cell dyeing contrast, and the sample density separation liquid can be widely applied to the exfoliative cytology specimens of gynecology, non-gynecology and the like and to a manual, semi-automatic or full-automatic liquid-based cell slide preparation process.

The invention discloses a method for automatically detecting the sedimentation rate of red blood cells. The method comprises the following steps of: setting the sampling time and the sampling times; upon the arrival of the sampling time each time, taking a picture of a blood sample, and accumulating the times for taking photos; obtaining the current liquid level position of the blood sample according to the pictures; comparing the accumulated times for taking pictures with the sampling times; and if the accumulated times for taking pictures is equal to the sampling times, obtaining the sedimentation rate of the red blood cells according to a plurality of the current liquid level positions. According to the method for automatically detecting the sedimentation rate of the red blood cells, which is provided by the embodiment of the invention, a mechanical transmission device is not needed, the mechanical design and software control are simple and feasible, and the computational complexity is low. The invention also discloses a device for automatically detecting the sedimentation rate of the red blood cells.

The invention discloses a plasma extraction device based on a microfluidic chip. The device comprises a microfluidic chip and a matched driving module. During use, whole blood is added into the microfluidic chip to leave the whole blood to stand for a while, almost red blood cells are settled in the bottom of a reaction chamber, and a small amount of red blood cells and plasma are located in the top layer of the reaction chamber; by means of an external mechanical action force, compression deformation on height of the reaction chamber of the microfluidic chip happens by means of the driving module, and the volume becomes small, so that the plasma and part of red blood cells are discharged out of the reaction chamber through a whole blood filter membrane, the red blood cells are blocked by micropores in the filter membrane while the plasma passes through the filter membrane, and is exported through the micropores of a transfer structural sheet, so that the plasma in the whole blood is extracted. The plasma extraction device based on the microfluidic chip is simple to operate, does not need complicated driving devices, and has an ideal plasma extraction efficiency. The plasma extraction device can be combined with other microfluidic detection modules to separate the plasma in a whole blood sample and detect a landmark of a disease.

The invention discloses a leflunomide tablet and a preparation technology and use thereof. The product is prepared from leflunomide, milk sugar, pregelatinized starch, hydroxypropyl methyl cellulose, carboxymethyl starch sodium, polysorbate-80, 12% slushing pregelatinized starch, magnesium stearate and a gastric soluble film coating premix. The production method comprises the steps of fabricating tablets and coatings, and the like. The leflunomide tablet disclosed by the invention is high in dissolution rate, good in stability, good in anti-inflammatory analgesic effect, and small in effect on erythrocyte sedimentation rate, and the total effective rate is 85%. The drug disclosed by the invention has the characteristics of being fewer in medication administration times, stable in plasma concentration, long in lasting time and the like.

An hemoglobin (Hb)-Dextran (Dx) conjugate having a molecular weight between 50 kd and 500 kD provides a blood substitute that results in acceptable erythrocyte sedimentation rate (ESR) and excretion rate (EXC) values. DxHb conjugates of the invention can be used for a variety of purposes as an alternative to blood.

The invention discloses a precise detecting method for obtaining erythrocyte sedimentation data. The precise detecting method comprises the following steps: step I. acquiring original erythrocyte sedimentation data by virtue of an analog-digital conversion acquiring device of an erythrocyte sedimentation deposition instrument, performing array loop traverse checking on the original erythrocyte sedimentation data, eliminating reading errors of the original erythrocyte sedimentation data, and screening the accurate original erythrocyte sedimentation data; step II, fitting the original erythrocyte sedimentation data through a least square method to generate a fitted curve to establish a mathematical model so as to obtain the initial erythrocyte sedimentation data; step III. subjecting the initial erythrocyte sedimentation data obtained in the step 2 to temperature compensation and error value elimination to obtain the final erythrocyte sedimentation data; and a step IV. feeding the calculated erythrocyte sedimentation data back to a data receiving end of the erythrocyte sedimentation deposition instrument for displaying.

The invention discloses a rapid erythrocyte sedimentation rate measuring method and a measuring device thereof. The measuring method comprises the steps that the geometric size of an erythrocyte sedimentation rate tube is modified, a photovoltaic detecting sensor is used for detecting the height value change of the sedimentation surface of erythrocyte in the modified erythrocyte sedimentation ratetube in the erythrocyte sedimentation process, an erythrocyte sedimentation rate Westergren value is obtained by a modern signal processing method and a regression algorithm, the measuring time of the rapid measuring method is half the time of a traditional Westergren method, and the correlation of the rapid measuring method with the Westergren method achieves 99%. The measuring device comprisesa microprocessor 01, a motor driving module 02, a motor execution module 03, a mechanical motion module 04 and a photovoltaic module 05. A rapid erythrocyte sedimentation rate analyzing meter is established by the rapid erythrocyte sedimentation rate measuring method and the measuring device thereof, a rapid erythrocyte sedimentation, rapid painting of an erythrocyte sedimentation rate value and erythrocyte sedimentation rate process curve can be realized, erythrocyte sedimentation rate sample measuring of a large batch can be automatically carried out, and the practical value is high.

A method for calibrating machines suitable to effect an analysis of a blood sample by measuring the erythrocyte sedimentation rate (ESR) and / or aggregation of the red corpuscles, wherein the measurement is effected by exploiting the optical density kinetics obtained from the measurement of the variation in the optical density of the blood sample in an interval of time, to include measuring in which, by the same machine with which the measurement of the optical density is effected on the blood sample, a measurement is effected of the optical density of two latexes, or turbidimetric samples. Each of the two latexes has a known optical density that is reproducible, measurable and different from each other. The method also calibrates in which the difference is calculated between the values of optical density of the latexes as obtained from the measurement performed by the machine and the known values of optical density, to determine at least one correction factor usable to calibrate machine.

The invention discloses a Widmannstatten method full-automatic erythrocyte sedimentation rate dynamic analyzer and a detection method, and the analyzer mainly comprises a transmission device used for placing a blood sample to be detected, the transmission device comprises a rotating disc, and the rotating disc is provided with a plurality of hole sites used for placing the blood sample; the sample introduction device is used for sucking a blood sample into the Widmanni erythrocyte sedimentation glass tube, and the blood sample enters the Widmanni erythrocyte sedimentation glass tube through the sample introduction needle, the valve group and the silicone tube connected with the Widmanni erythrocyte sedimentation glass tube; the detection device is used for detecting the liquid level height of the blood sample in the Widmanni erythrocyte sedimentation glass tube; the cleaning device is used for cleaning and blow-drying the Widmannstan erythrocyte sedimentation glass tube after the test is finished. By adopting the analyzer and the detection method, the working efficiency can be improved, the reading is accurate, the operation is simple, the biological hazard is reduced, and the cleaning is convenient.

The invention discloses a preparation method of hydroxyapatite by using red blood cells as bioreactor, which belongs to the technical field of synthesis of inorganic nano materials. The technical proposal of the invention comprises the following main points that the upper plasma is removed from animal blood or human blood by centrifugation and the remainder is washed with physiological saline several times to obtain erythrocyte sedimentation, the erythrocyte sedimentation is added to the physiological saline containing soluble barium salt and the reaction is carried out under the condition of ice-water bath, then the mixture obtained by the reaction is centrifuged and washed after the reaction, the erythrocyte sedimentation obtained by the centrifugation is collected and resuspended in the PBS buffer solution(PH=9) to carry out the reaction, and functional red blood cells containing hydroxyapatite are obtained by centrifugation after the reaction, then the hydroxyapatite nanoparticles with an average particle size of 5.6 nm are prepared by the crush, separation and purification of the functional red blood cells. The invention has a series of advantages of green environmental protection, simple operation, low cost and good biocompatibility.

The invention discloses a plasma extraction device based on a microfluidic chip. The device comprises a microfluidic chip and a matched driving module. During use, whole blood is added into the microfluidic chip to leave the whole blood to stand for a while, almost red blood cells are settled in the bottom of a reaction chamber, and a small amount of red blood cells and plasma are located in the top layer of the reaction chamber; by means of an external mechanical action force, compression deformation on height of the reaction chamber of the microfluidic chip happens by means of the driving module, and the volume becomes small, so that the plasma and part of red blood cells are discharged out of the reaction chamber through a whole blood filter membrane, the red blood cells are blocked by micropores in the filter membrane while the plasma passes through the filter membrane, and is exported through the micropores of a transfer structural sheet, so that the plasma in the whole blood is extracted. The plasma extraction device based on the microfluidic chip is simple to operate, does not need complicated driving devices, and has an ideal plasma extraction efficiency. The plasma extraction device can be combined with other microfluidic detection modules to separate the plasma in a whole blood sample and detect a landmark of a disease.

The invention discloses a tenoxicam tablet and a production process and application thereof. The tenoxicam tablet is prepared from tenoxicam, lactose, pregelatinized starch, hydroxypropylmethyl cellulose, carboxymethyl starch sodium, polysorbate-80, 15% starching pregelatinized starch, micro-powdered silica gel, magnesium stearate and a stomach dissolved type film coating premix. The production process comprises the steps of preparing a tablet core, coating and the like. According to the tenoxicam tablet, shown by treating 30 cases of rheumatoid arthritis, the anti-inflammatory and analgesic treatment effect is good, however, the influence on erythrocyte sedimentation rate is not great, and the total effective rate is 83%; the tenoxicam tablet has the characteristics that the number of medicine taking is small, the plasma concentration is stable, the duration is long and the like.

The embodiment of the invention discloses a sample analyzer, a signal acquisition method and a computer storage medium. When an acquired optical signal is used for erythrocyte sedimentation analysis, an adverse effect on an erythrocyte sedimentation analysis result is avoided. According to the sample analyzer provided by the embodiment of the invention, a control module controls opening or closing of an optical signal generation module, an optical signal acquisition module and a heating module which are in communication connection with the control module, the control module controls the optical signal acquisition module to acquire optical signals beyond the time period when the heating module is opened or closed to bring interference signals, and acquisition of the optical signals can be not interfered; and in the time period when the heating module is turned on or turned off to bring the interference signals, the control module controls the optical signal acquisition module to stop acquiring the optical signals, so that the interfered optical signals are prevented from being acquired. By performing linkage control on a plurality of modules such as the optical signal acquisition module and the like, in the whole signal acquisition process, pure and interference-free optical signals can be acquired, and subsequent signal processing and analysis processes are facilitated.

The invention discloses a chrysanthemum total flavone granule, which is made of the following raw materials in the weight ratio: sucrose powder: dextrin: dry extract powder of the total flavonoids of chrysanthemum = 0.5-1.5: 1.5-2.5: 0.5-1.5 . The present invention prepares chrysanthemum total flavonoids into granules. The granules have good formability, properties, particle size, water content, solubility, etc. all meet the requirements, and the quality is controllable and the stability is good. The color changes, the smell is lost, the content of active ingredients decreases, and the color of the item in the soaking process does not turn green. Chuju total flavonoids granules can significantly prolong the coagulation time of rats with cold coagulation and blood stasis syndrome, reduce the length of thrombus in vitro and dry and wet weight, reduce hematocrit, slow down erythrocyte sedimentation rate, and increase serum NO content. The product is easy to drink and carry, easy to store, has no toxic and side effects, and has the characteristics of preventing, treating and improving abnormal blood rheology in rats with blood stasis.

The invention discloses a preparation method of nano barium carbonate by using red blood cells as bioreactor, which belongs to the technical field of synthesis of inorganic nano materials. The technical proposal of the invention comprises the following main points that the upper plasma is removed from animal blood or human blood by centrifugation and the remainder is washed with physiological saline several times to obtain erythrocyte sedimentation, the erythrocyte sedimentation is added to the physiological saline containing soluble barium salt and the reaction is carried out under the condition of ice-water bath, then the mixture obtained by the reaction is centrifuged and washed after the reaction, the erythrocyte sedimentation obtained by the centrifugation is collected and resuspended in the physiological saline containing sodium carbonate to carry out the reaction, and functional red blood cells containing the barium carbonate are obtained by centrifugation after the reaction, then the barium carbonate nanoparticles with an average particle size of 4.2 nm are prepared by the crush, separation and purification of the functional red blood cells. The invention has a series of advantages of green environmental protection, simple operation, low cost and good biocompatibility.

The invention discloses a preparation method of hydroxyapatite by using red blood cells as bioreactor, which belongs to the technical field of synthesis of inorganic nano materials. The technical proposal of the invention comprises the following main points that the upper plasma is removed from animal blood or human blood by centrifugation and the remainder is washed with physiological saline several times to obtain erythrocyte sedimentation, the erythrocyte sedimentation is added to the physiological saline containing soluble barium salt and the reaction is carried out under the condition of ice-water bath, then the mixture obtained by the reaction is centrifuged and washed after the reaction, the erythrocyte sedimentation obtained by the centrifugation is collected and resuspended in the PBS buffer solution(PH=9) to carry out the reaction, and functional red blood cells containing hydroxyapatite are obtained by centrifugation after the reaction, then the hydroxyapatite nanoparticles with an average particle size of 5.6 nm are prepared by the crush, separation and purification of the functional red blood cells. The invention has a series of advantages of green environmental protection, simple operation, low cost and good biocompatibility.

An erythrocyte sedimentation rate log capable of quickly and easily measuring an erythrocyte sedimentation rate in the blood by measuring the number of erythrocytes in the blood passing through a branched channel from a small amount of blood in an electrical manner without an additional driving means is disclosed. The erythrocyte sedimentation rate log includes a main body formed of an upper plate and a lower plate, a blood introduction part formed in the main body to include an inlet and an outlet formed at one side of the upper plate of the main body, a channel disposed at one side of the blood introduction part to communicate with the blood introduction part such that erythrocytes in blood pass through the channel, and a current supply part installed in the channel to supply constant current to cause an electrical resistance in erythrocytes.

The present invention relates to the use of a combination of: (i)a macromolecular erythrocyte sedimentation enhancer, and (ii) dimethyl sulphoxide (DMSO), dimethylglycine (DMG) and / or valine; to recover non-erythrocyte blood cells from a blood cell-containing sample and / or to prime non-erythrocyte blood cells to protect their integrity in subsequent cryopreservation step(s).

The invention discloses an efficient and rapid erythrocyte sedimentation agent and a preparation method thereof. The method relates to the field of clinical examination, medical research and other fields using whole blood samples of people. The preparation method comprises the following steps of adopting starch as a raw material, carrying out carboxylation on the raw material under a certain condition to prepare high substituted modified starch, and co-mixing the modified starch and sodium chloride according to a certain proportion to prepare the erythrocyte sedimentation agent. The erythrocyte sedimentation agent can be used for quickly and efficiently separating and sedimentating erythrocyte from whole blood, is high in safety, cheap in raw material which is easy to get, low in production cost, simple, convenient and easy in preparation process, and suitable for industrial production in large scale.

The present invention relates to the use of a combination of: (i)a macromolecular erythrocyte sedimentation enhancer, and (ii) dimethyl sulphoxide (DMSO), dimethylglycine (DMG) and / or valine; to recover non-erythrocyte blood cells from a blood cell-containing sample and / or to prime non-erythrocyte blood cells to protect their integrity in subsequent cryopreservation step(s).

A bone marrow fluid of a patient is sampled, housed in a collection tube 11 and conveyed into an isolator in a sterile state. In the isolator, the bone marrow fluid in the collection tube is pipetted into test tubes and culture tube, etc. To the bone marrow fluid in the tubes, an erythrocyte sedimentation agent is added. After precipitating erythrocytes, the supernatant is collected. From the collected supernatant, a bone marrow cell-containing fraction for liver regeneration is concentrated and pipetted into flasks which are conveyed into an incubator to start the cell-culture. From the isolator system, test tubes are taken out. The bone marrow fluid in the test tubes and the concentrate are subjected to a safety test, etc.