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Sample density separation liquid and preparation method thereof

A density separation and sample technology, applied in the field of cell separation in biomedical technology, can solve problems such as affecting the observation of samples, and achieve the effects of accelerating the sinking speed, increasing the effective density, and accelerating the speed of cell water loss.

Active Publication Date: 2016-10-26
HANGZHOU HEALTHSKY BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The traditional commonly used sample density separation liquid is Ficoll separation liquid. The main component of Ficoll separation liquid is a synthetic sucrose polymer, referred to as polysucrose. The red blood cells that have not been completely lysed by the preservation solution at the layered part of the separation solution will be in contact with polysucrose to form a string of money and deposit at the bottom of the tube to mix with the sinking cells of diagnostic significance (epithelial cells, tumor cells), affecting Observation of samples after subsequent film production

Method used

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  • Sample density separation liquid and preparation method thereof
  • Sample density separation liquid and preparation method thereof
  • Sample density separation liquid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-4

[0036] Embodiment 1-4: a kind of sample density separation liquid, it is prepared by the following method:

[0037] (1) mixing ethanol, sodium chloride, tea saponin and double distilled water of formula quantity, stirring and dissolving, obtain clear solution;

[0038] (2) Add glycerin to the clear solution obtained in step (1) at 18°C-22°C, and stir evenly to obtain the product;

[0039] Each composition of embodiment 1-4 and consumption thereof are as shown in table 1.

[0040] The composition of table 1 embodiment 1-4 and parts by weight thereof

[0041]

Embodiment 5

[0043] Test object: a sample density separation liquid in Example 3 of the present application was used as a test sample, and a commercially available Ficoll sample density separation liquid was used as a control sample.

[0044]Test content: Using conventional gynecological examination methods, exfoliated cells in the transitional zone of the cervix were extracted, and the samples were preserved with the preservation solution produced by the American CCID company. Fully automatic liquid-based cell staining system for film preparation and microscopic examination and photography.

[0045] test results:

[0046] Microscopic examination photos of cervical normal squamous epithelial cells processed by the sample density separation liquid of Example 3 of the present application are as follows: figure 1 shown;

[0047] Microscopic examination photos of cervical normal squamous epithelial cells treated with commercially available Ficoll sample density separation fluid are as follow...

Embodiment 6

[0052] Test object: a kind of sample density separation liquid of Example 1-4 of the present application was used as a control sample.

[0053] Test content: Using conventional gynecological examination methods, the exfoliated cells in the transition zone of the cervix of normal women were extracted, and the samples were preserved with the preservation solution produced by the American CCID company. The 4800 automatic liquid-based cell staining system is used for film preparation and microscopic examination, and the experimental temperature and natural sedimentation time are the same.

[0054] Test results: Table 2

[0055] Example

[0056] As can be seen from Table 2, compared with Comparative Example 1 and Comparative Example 2, the number of microscopic red blood cells in Comparative Example 3 and Comparative Example 4 is 0, which shows that when the mass percentage concentration of tea saponin in the above-mentioned system is 0.16%, The sample density separation...

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Abstract

The invention discloses sample density separation liquid which comprises the following components in percentage by mass: 5-10% of glycerin, 3-5% of ethanol, 3-5% of sodium chloride, 79.8-88.9% of double distilled water and 0.1-0.2% of tea saponin. By adopting the sample density separation liquid, the cells of diagnostic value can naturally sedimentated more quickly while the erythrocyte sedimentation interference is reduced, thus a microscopic examination slice has clearer microscopic examination view and good cell dyeing contrast, and the sample density separation liquid can be widely applied to the exfoliative cytology specimens of gynecology, non-gynecology and the like and to a manual, semi-automatic or full-automatic liquid-based cell slide preparation process.

Description

technical field [0001] The invention relates to the field of cell separation in biomedical technology, in particular to a sample density separation liquid. Background technique [0002] Cervical cancer is one of the most common malignant tumors in women and the main killer of women's health. Its incidence rate is second only to breast cancer. According to statistics, there are about 500,000 new cases of cervical cancer in the world every year, of which 80% occur In developing countries, early screening of cervical cancer is therefore particularly important. [0003] At present, the commonly used early screening method for cervical cancer is liquid-based cytology, which uses a liquid-based thin-layer cytology detection system to detect cervical cells and conduct cytological classification diagnosis. It is currently a more advanced cervical cancer cell in the world. Compared with the traditional cervical smear and Pap smear, it significantly improves the satisfaction of speci...

Claims

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Application Information

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IPC IPC(8): C12N5/071A01N1/02
CPCA01N1/0205C12N5/0625C12N2500/05C12N2500/30C12N2500/35
Inventor 王云皓吴屹豪
Owner HANGZHOU HEALTHSKY BIOTECH CO LTD
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