304 results about "Cell staining" patented technology

Methods for efficient production of recombinant AAV employ a host cell which comprising AAV rep and cap genes stably integrated within the cell's chromosomes, wherein the AAV rep and cap genes are each operatively linked to regulatory sequences capable of directing the expression of the rep and cap gene products upon infection of the cell with a helper virus, a helper gene, and a helper gene product. A method for producing recombinant adeno-associated virus (rAAV) involves infecting such a host cell with a helper virus, gene or gene product and infecting the infected host cell with a recombinant hybrid virus or plasmid vector containing adenovirus cis-elements necessary for replication and virion encapsidation, AAV sequences comprising the 5′ and 3′ ITRs of an AAV, and a selected gene operatively linked to regulatory sequences directing its expression, which is flanked by the above-mentioned AAV sequences.

The present invention provides compositions and methods for targeted cleavage of cellular chromatin in a region of interest and / or homologous recombination at a predetermined site in cells. Compositions include fusion polypeptides comprising a TAL effector binding domain and a cleavage domain. The cleavage domain can be from any endonuclease. In certain embodiments, the endonuclease is a Type IIS restriction endonuclease. In further embodiments, the Type IIS restriction endonuclease is FokI.

A full set of 24 chromosome-specific probes to analyze single cells or cell organelles to test for abnormalities is described. When used in an assay based on sequential hybridization, the full set is comprised of three subsets of chromosome-specific probes with each set comprised of 8 different probes. Also described are assays using a set of probes to analyze single cells and cellular organelles to accurately determine the number and type of targeted human chromosomes in various types of cells and cell organelles, such as tumor cells, interphase cells and first polar bodies biopsied from non-inseminated oocytes. Methods of selection or generation of suitable probes and hybridization protocols are described, as are preferred probes for frill set of 24 chromosome-specific probes to target all 24 human chromosomes are described in the Tables.

The invention relates to a method for producing stem cells having an increased development potential from somatic stem cells, wherein a tissue sample comprising somatic stem cells or a body fluid sample comprising somatic stem cells is taken from an organism, wherein from this tissue sample or body fluid sample as an option somatic stem cells are isolated and / or cultivated, and wherein the thus obtained somatic stem cells are treated with a substance modulating the methylation of the DNA of the cells or a substance modulating the acetylation of chromatin of the cells.

Solution-based methods for identifying an MHC-binding peptide or measuring affinity of MHC-binding peptides for an MHC monomer, or modified MHC monomer by incubating at least one MHC monomer or modified MHC monomer having a bound template MHC-binding peptide, an excess amount of a competitor peptide, and a tracer MHC-binding peptide tagged with a detectable label so as to allow competition binding between the three peptides At least a portion of the competitor peptide exchanges with the template peptide and a difference in signal produced by the detectable label in the total sample as compared with signal produced solely by monomers after the competition assay is obtained and used to calculate affinity of the competitor peptide for the monomer. These methods are useful in peptide discovery programs and exchanged monomers can be further tested for activity in tetramer cell staining assays.

The invention relates to a study on in vivo fluid shearing force simulation cell behaviors on the basis of a microfluidic chip system. An external precise fluid injection pump is used as a motive power source, a microfluidic chip soft etching technology is used as the basis, and the fluid resistance principle is utilized for designing and manufacturing a microfluidic passage resistance network, so cells in a microfluidic chip cell culture microchamber can feel fluid shearing force with different intensities, and further, the cell behavior change is studied. Through the microfluidic chip system, the process of the inoculation and long-term culture of cells, the fluid shearing force stimulation on the cells and the cell dyeing and analysis and detection are integrated on a functionalization chip to be completed, and the cell behavior analysis and the in vivo ultra-low fluid shearing force simulation on a microfluidic chip platform are realized.

Methods for differentially identifying cells in an instrument employ compositions containing a combination of selected antibodies and fluorescent dyes having different cellular distribution patterns and specificities, as well as antibodies and fluorescent dyes characterized by overlapping emission spectra which form non-compensatable spectral patterns. When utilizing the compositions described herein consisting of fluorescent dyes and fluorochrome labeled antibodies with overlapping spectra that cannot be separated or distinguished based upon optical or electronic compensation means, a new fluorescent footprint is established. This new fluorescent footprint is a result of the overlapping spectra and the combined cellular staining patterns of the dyes and fluorochrome labeled antibodies chosen for the composition. The new fluorescent footprint results in histogram patterns that are useful for the identification of additional cell populations or subtypes in hematological disease.

The present invention relates to a method and a system for automatically recognizing rare cells. The method comprises: injecting a cell enrichment liquid carrying rare cells into a prepared coating-slice device, staining the cells according to a standard immunostaining process, and carrying out multi-fluorescence channel shooting through a fluorescence microscope to obtain a fluorescence image; treating the fluorescence image into a clean image strictly having a background gray value of zero and retaining the true fluorescence value in the cell contour, and carrying out cell contour extraction on the clean image; and carrying out statistics on the average fluorescence intensity value of various extracted contour channels corresponding to the multi-fluorescence channel, and determining the rare cells according to the statistics results. According to the present invention, the obtained fluorescence image is treated to strictly achieve the background gray value of zero while the true fluorescence value in the cell contour is retained, such that the influence of the background noise on the cell contour extraction is minimized; and for the fluorescence image obtained through the multi-fluorescence channel, the cells are confirmed and recognized from more parameters so as to improve the staining recognizing of the rare cells.

The invention discloses an automatic identification and statistics method for white blood cells in a gynecologic microscopic image. A color image is firstly converted into a gray image, and an image segmentation method is used to obtain a template for the white blood cells; AND-operation is carried out on the template and the gray image; each small area is segmented; and by using the roundness of the white blood cells, the length-width ratio of an external rectangle, the area and the ratio of a cell nucleus to the cell, identification on the white blood cells is realized. Automatic identification and statistics are realized, clinical requirements are met, artificial detection and cell identification by using cell staining are replaced, and the manpower and the material resources are saved greatly.

The invention belongs to the technical field of bio-analytical detection and discloses a fluorescent compound with aggregation-induced luminescence properties and application thereof to the cell fluorescence imaging field. The fluorescent compound with the aggregation-induced luminescence properties adopts one of structures shown as a formula 1 to a formula 4. The fluorescent compound with the aggregation-induced luminescence properties almost does not emit light or emit weak fluorescence in a solution state, can obtain the strong fluorescence emission aggregation-induced luminescence properties in solid state and aggregation states, has room temperature phosphorescent emission properties in a crystalline state, is simple to prepare and wide in luminous spectrum range, is applied to cell fluorescence imaging, biological analysis and other fields, has the characteristics of being sensitive and quick in cell dyeing, small in cytotoxicity, excellent in fluorescence signal anti-photobleaching properties and capable of in-situ real-time monitoring, and has practical application values.

Described herein are compounds, methods, and kits for long-term tracking of cell proliferation, differentiation, and / or function. The compounds of the present invention are novel cell-tracking reagents, efficiently excitable with a 405-nm violet laser, that provide bright fluorescence intensity, uniform cell staining, and good retention within cells as well as low toxicity toward cells.

The present invention relates to methods for detecting the chromatin state of a cell based on recording a super resolution image of nucleosome organization and correlating said imaged with size of nucleosomal clutches, nucleosomal density and / or number of nucleosomes per nucleosomal clutches. Additionally, the invention relates to a kit comprising a first antibody capable of specifically binding to a histone protein and a photo switchable fluorophore linked-secondary antibody and the use of the kit of the invention for detecting the chromatin state of a cell and isolating a cell in an open chromatin state or in a close chromatin state. The invention also relates to a device adapted to detect the chromatin state of a cell.

MicroRNA genes are associated with regulatory elements of living cells of all species. The perturbations of the expression of these genes and their gene products in the cell or genomic structure or chromosomal architecture of a cell provide specific signatures on the condition of the cell and even the organism. Evaluation of miR gene expression can therefore be used to indicate the presence and state of specific cell types and / or their state of differentiation relative to their surrounding tissue. The present invention relates to the identification of a stem cell-specific signature or signatures composed of protein and / or nucleic acid markers expressed by virtue of the position of a cell or cells in the time line of its / their development and the impact of the cells' environment on this signature as it relates to the cells' stem cell potential. The composition and combination of these signatures provides a means of identifying, manipulating and differentiating said adult stem cells and thus, their acquisition and utilization in research, diagnosis, and therapy of normal and pathological conditions.

The invention discloses liquid-based thin-layer cell preservation solution and use thereof. The preservation solution comprises 5 to 28 volume percent of aldehyde solution, 0.1 to 3 volume percent of acid solution, 0.1 to 6 mass volume percent of chloride, 0.1 to 3 mass volume percent of tris(hydroxymethyl)aminomethane and the balance volume percent of double distilled water. The preservation solution of the invention can remove red blood cells, preserve white blood cells, timely fix the original state of cells of diagnostic value, soften mucilage and epidermal tissues, weaken mucous effect and separate effective cells from mucilage. The microscopic examination piece manufactured by using the preservation solution has clear microscopic examination view field and high cellular staining contrast and is widely suitable for gynecological and non-gynecological exfoliative cytological specimens and manual, semi-automatic or fully-automatic liquid-based cell substrate making processes. The production cost of the preservation solution is low, so the application and promotion of a liquid-based thin-layer cell detection technique are convenient.

The invention uses synergetic combination effect of multiple cytoactive protection solution and a stabilizer, and a protective agent provides nutrition components and living environment required by maintaining normal physiological activity for blood cells for anaerobic glycolysis; the stabilizer containing cytolemma and cytoplasm fixed substances with lower concentration can slowly permeate the cytolemma to act on the cytoplasm and firmly combine with biomolecule of cell components such as protein, enzyme, lipid and the like effectively, thus enabling white blood cells, red blood cells and blood platelets to be fixed in different degrees and get effective protection and enabling blood cells to keep apparent morphological characteristics thereof. The blood cells in protection and good stabilized treatment is subjected to assignment according to traceability link delivery procedures and reference methods thereof, and can be used as blood cell analyzer calibrators for clinic usage. The invention uses stem complete blood cell (CBC) from human beings or animals, thus having good stability; the invention can be used in microscope manual counting and cell staining analysis, and has longerexpirydate compared with the current full blood cell calibrators.

The invention relates to a carbon quantum dot fluorescence labeling material with orange peels used as a carbon source as well as a preparation method and an application of the carbon quantum dot fluorescence labeling material. The orange peels are used as a raw material, and a simple method for one-step synthesis of a high-fluorescence-quantum-efficiency carbon quantum dot is established. The method comprises steps as follows: the orange peels are mixed with water; then a mixed liquid is mixed with concentrated sulfuric acid; the pH is adjusted by the aid of NaOH, and undissolved residues are removed in a centrifugal manner; then dichloromethane is added to a centrifuged supernate, and a non-fluorescence fat-soluble substance is removed in the centrifugal manner; finally, the centrifuged supernate passes through a film, and the fluorescence labeling material is prepared. According to the method, high-temperature and high-pressure reactions are not required to be performed by equipment such as plasma emitters, hydrothermal reaction kettles, muffle furnaces and the like during synthesis, reaction conditions are mild, operations are simple and convenient, the cost is very low, the method can be implemented in ordinary laboratories, the synthesized fluorescence labeling material lays a foundation for the further application to work such as bio-labeling, heavy metal analysis, antibody labeling, cell staining and the like.

The invention relates to an evaluation method for measuring cell DNA damage caused by soluble heavy metal. The method includes the following steps that firstly, tested cells are processed through a soluble heavy metal solution; secondly, the processed tested cells are fixed and are subjected to cell permeabilization; thirdly, the tested cells having been subjected to cell permeabilization are dyed; fourthly, detection is performed through a high-content imaging system and analysis is performed. The evaluation method for measuring the cell DNA damage caused by the soluble heavy metal is convenient to operate, high in sensitivity and high in detection speed and has high specific pertinence, and research on the genetic toxicity of the heavy metal is supplemented and expanded.

The invention relates to a cell staining slide making machine and a staining slide making method. The cell staining slide making machine comprises a first rotating disc, a second rotating disc located on the periphery of the first rotating disc, and at least one cantilever which is located above the first rotating disc and the second rotating disc and can move horizontally. A plurality of sample racks used for carrying samples are arranged on the first rotating disc in the circumferential direction of the first rotating disc, and a plurality of slide racks used for carrying slides are arranged on the second rotating disc in the circumferential direction of the second rotating disc; or a plurality of slide racks are arranged on the first rotating disc in the circumferential direction of the first rotating disc, and a plurality of sample racks are arranged on the second rotating disc in the circumferential direction of the second rotating disc. First tube racks and second tube racks are arranged on the cantilevers and can move vertically relative to the cantilevers, the first tube racks are used for installing suction nozzles or staining tubes, and the second tube racks are used for installing staining tubes or suction nozzles. The risk of cross contamination can be effectively avoided, and the working efficiency is high.

The invention belongs to the technical field of fluorochrome and discloses preparation and application of pH-response type rhodamine grafted lignin-based fluorochrome. The general structure of the fluorochrome is shown in the formula (1). The preparing method comprises the steps of dissolving 100 parts of lignin or lignosulfonate in water or an organic solvent and water mixed solution, adding alkali to regulate PH value to be 9-14, adding 1-20 parts of chloro-alkylene oxide for reaction lasting 0.5-24 h at 25-50 DEG C, adding alkali after reaction to maintain PH value at 9-14, adding 1-25 parts of rhodamine amido derivative organic solvent solution, increasing temperature to 60-80 DEG C for reaction lasting 0.5-24 h, conducting sulfonation after reaction, and conducting dialysis membrane or cation-anion resin purification to obtain the product. The product has AIE activity, can be used for in vitro cell staining, and has a strong inhibition effect on cancer cells.

The invention discloses a system using an extremely low frequency pulsed electric field to treat seeds, which comprises an extremely low frequency high voltage pulse generator, wherein a copper plate is respectively connected with two poles of the extremely low frequency high voltage pulse generator, the two copper plates are placed horizontally and serve as electrodes, and organic glass which is used for placing seeds is arranged between the two copper plates. A method which adopts the system to treat seeds includes the following steps: firstly, cleaning and sterilizing the seeds, killing surface microorganisms, and immersing the seeds in water for 3 to 24 hours; and then placing seeds which absorb water and germinate on the organic glass, and starting the extremely low frequency high voltage pulse generator to treat the seeds. According to the system using the extremely low frequency pulsed electric field to treat seeds and the treatment method, no pretreatment of seeds in any mode is required, only the extremely low frequency pulsed electric field is used for facilitating germination and growth of seeds, environmental pollution is avoided, nucleuses are not affected, chromosome variation of cells and environment hazards are avoided, the system and the method are simple and practical, and the effects are obvious.

The invention discloses a breeding method for triploid cassava. The triploid cassava is bred through the following steps: inducing diploid cassava with chemical medicines so as to double cell chromosomes, manually cutting and separating branches of the induced diploid cassava a plurality of times so as to breed branches of totally mutated tetraploid cassava, carrying out softwood or greenwood cutting propagation on the branches so as to breed tetraploid lines, and hybridizing the tetraploid lines with diploid cassava so as to breed triploid cassava. The advantages of the invention are as follows: colchicine is utilized in the invention to induce chromosome double of cassava and goal-directed breeding is carried out; the breeding method has the characteristics of a simple process, easy operation, a high success rate, short time and greatly improved efficiency in breeding new species of cassava.

The invention discloses a dyeing device. The dyeing device comprises a workbench and a moving arm which is mounted on a cross beam at the upper part of the workbench in a sliding manner, wherein a suction nozzle which can be assembled and disassembled is mounted on the lower end surface of the moving arm, a rotating disk which can rotate in forward and reverse directions is arranged on the workbench, a plurality of dyeing bins are arranged on the rotating disk, a plurality of liquid distribution bottles are further arranged on the workbench, and the actions of the moving arm and the rotating disk are controlled by a controller arranged on the workbench. According to the dyeing device disclosed by the invention, the rotating disk is arranged, the dyeing bins are mounted on the rotating disk, the liquid medicine is sucked from the liquid distribution bottles through the moving arm and the suction nozzle which can be assembled and disassembled, through the action of a valve, the liquid medicine in the suction nozzle can be quantitatively added into the dyeing bins, after the dyeing of cells is completed, the liquid medicine in the dyeing bins can be rapidly drained through the centrifugal force generated by rotation of the rotating disk, and the dyed cells can be prevented from being sucked away. The dyeing device disclosed by the invention can fast add and move liquid, can shorten the duration of a cell dyeing experiment, and can be widely used as a cell dyeing device.

The present disclosure relates to chemical dyes useful for staining and imaging of cells. In particular, the disclosure relates to compound of Formula I, method of preparation thereof, and it's use as a fluorescent probe for staining and / or imaging mitochondria in cells, tissues or animals, resulting in a range of applications including, but not limiting, to sensing local ordering or viscosity of mitochondria, tracking mitochondrial mobility, comparing & evaluating mitochondrial function, local ordering, microviscosity and dynamics. Said dyes have additional advantages including, but not limiting, to low toxicity, longer shelf-life, generate little or no reactive species upon long term light irradiation and do not perturb the functionality of the mitochondria in cells compared to prior art dyes.

The invention discloses a target cell staining kit for displaying the proliferative activity of a cell and a use method thereof. The kit comprises I type and II type which respectively comprise an enzyme substrate, test paper, enzyme stationary liquid, phosphate buffer solution, Mayer hematoxylin and the like. The staining method comprises the steps of preparing pieces, fixing, staining and the like. The method can display acid phosphatase in exfoliative cells on smears such as exfoliative cervical cells, esophagus brush sheet cells and the like and search abnormal proliferated cells according to the target. Compared with the traditional Pasteur smear method, the method has the advantages of higher sensitivity for detecting cancer and precancerous lesions and similar specificity, therefore, the invention can be used for cytology screening of cervical carcinoma, esophageal cancer, lung cancer and other malignant tumors.

The invention provides a blood cell component analysis instrument and a method. The instrument comprises a sample absorbing and transfer unit, a dyeing liquid adding unit, a stirring and smearing unit, a cleaning unit, a microscanning unit, an image processing unit and a control unit. The sample absorbing and transfer unit is used for absorbing a quantitative blood sample to a glass slide; the dyeing liquid adding unit is used for adding the dyeing liquid to dye cells and dilute the blood sample; a reverse T-shaped stirring and smearing unit is used for mixing the blood sample and the dyeing liquid uniformly and smearing the mixed blood sample; the microscanning unit is used for performing microscopic examination on the smear; the instrument automatically and continuously performs axial scanning on the unclear targets for many times; the image processing unit processes the scanning image and selecting the images with clearest cells or granules in the multi-focus images into a whole image; and the analysis instrument is simple to operate, low in failure rate, accurate in result and few in consumed material reagent, and can perform quantitative analysis on the blood cells conveniently and accurately.

A kit used for quickly diagnosing concentration of semen consists of semen collection box, cell-stain liquid and test plate. It is featured as forming said semen collection box by plastic collection box and semen liquefier; forming said test plate by shell and base plate including water absorption paper, filtering film and standard color code; arranging two holes of test hole and comparison hole at center of shell.

Disclosed are HSV-1 amplicons that supply all necessary helper functions required for rAAV packaging and methods for their use. These HSV-1 amplicons have been shown to be capable of rescuing and replicating all forms of rAAV genomes including rAAV genomes introduced into cells by infection of rAAV virions, rAAV genomes transfected into cells on plasmids or proviral rAAV genomes integrated into cellular chromosomal DNA. Also provided are methods for preparing high-titer rAAV vector compositions suitable for gene therapy and the delivery of exogenous polynucleotides to selected host cells.

Systems and methods are used to display cell structures of a biological cell. A plurality of cell structures of a biological cell is stored and for each cell structure of the plurality of cell structures one or more stain colors are stored. A selected cell structure is received from an input device. One or more stain colors of the selected cell structure are retrieved. The one or more stain colors of the selected cell structure are displayed. A selected stain color is received from the input device. The selected cell structure is displayed in the selected stain color in an exemplary cell image. Further, a three-dimensional image of a biological cell is stored. The three-dimensional image is displayed on a display that includes a touch screen. A movement selection is received from the touch screen. The three-dimensional image is displayed on the display according to the movement selection.