Liquid-based thin-layer cell preservation solution and use thereof

Abstract

The invention discloses liquid-based thin-layer cell preservation solution and use thereof. The preservation solution comprises 5 to 28 volume percent of aldehyde solution, 0.1 to 3 volume percent of acid solution, 0.1 to 6 mass volume percent of chloride, 0.1 to 3 mass volume percent of tris(hydroxymethyl)aminomethane and the balance volume percent of double distilled water. The preservation solution of the invention can remove red blood cells, preserve white blood cells, timely fix the original state of cells of diagnostic value, soften mucilage and epidermal tissues, weaken mucous effect and separate effective cells from mucilage. The microscopic examination piece manufactured by using the preservation solution has clear microscopic examination view field and high cellular staining contrast and is widely suitable for gynecological and non-gynecological exfoliative cytological specimens and manual, semi-automatic or fully-automatic liquid-based cell substrate making processes. The production cost of the preservation solution is low, so the application and promotion of a liquid-based thin-layer cell detection technique are convenient.

Description

technical field [0001] The invention relates to a cell treatment liquid, in particular to a liquid-based thin-layer cell preservation treatment liquid and its application. Background technique [0002] The commonly used preparation method in cell detection is the Pap cell smear method. The doctor needs to manually smear the cell sample obtained with the collector on the microscope. The smear made by this traditional manual method only collects a maximum of 20% of the cells, while more than 80% of the cell samples remain on the sampler and are discarded together with the sampler. In addition, more than 40% of the smears will become cloudy due to the influence of blood, mucus and inflammatory tissue, as well as artifacts caused by untimely fixation. These shortcomings are responsible for the inaccurate results of most routine Pap tests. [0003] The liquid-based thin-layer cytology method is an internationally advanced cytological examination technology developed in recent y...

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Problems solved by technology

[0004] At present, the imported cell preservation treatment solution is expensive, and the cost of a liquid-based thin-layer cell inspection is about 180 yuan. Such a high inspection fee makes it difficult to promote this technology in low-income areas; among similar domestic products, such as the invention patent ZL200610021857.7 " The liquid-based thin-layer cell preparation method in "Simple Thin-Layer Liquid-Based Cytological Examination Method", the cell preservation solution and the treatment solution are separated, the preparation process is cumbersome and the efficiency is low

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