528 results about "Cytology" patented technology

Raman molecular imaging (RMI) is used to detect mammalian cells of a particular phenotype. For example the disclosure includes the use of RMI to differentiate between normal and diseased cells or tissues, e.g., cancer cells as well as in determining the grade of said cancer cells. In a preferred embodiment benign and malignant lesions of bladder and other tissues can be distinguished, including epithelial tissues such as lung, prostate, kidney, breast, and colon, and non-epithelial tissues, such as bone marrow and brain. Raman scattering data relevant to the disease state of cells or tissue can be combined with visual image data to produce hybrid images which depict both a magnified view of the cellular structures and information relating to the disease state of the individual cells in the field of view. Also, RMI techniques may be combined with visual image data and validated with other detection methods to produce confirm the matter obtained by RMI.

A method and system of differentially manipulating cells where the cells, suspended in a fluid, are irradiated with substantially monochromatic light. A Raman data set is obtained from the irradiated cells and where the data set is characteristic of a disease status. The data set is assessed to identify diseased cells. A Raman chemical image of the irradiated cells is also obtained. Based on the assessment and the Raman chemical image, the fluid in which the cells are suspended is differentially manipulated. The diseased cells are directed to a first location and other non-diseased cells are directed to a second location as part of the differential manipulation. The diseased cells may be treated with a physical stress, a chemical stress, and a biological stress and then returned to a patient from whom the diseased cells were obtained prior to the irradiation.

A solution for preservation and / or storage of a cell or tissue is described. This simple nonaqueous composition can have 10% polyethylene glycol and 90% methanol. It can be used at room temperature. Special chemicals, equipment, and techniques are not needed. Tissue preserved with and / or stored in the solution can be processed for cytology or histology, including chemical staining and / or antibody binding, by a variety of methods; antigen, DNA, and RNA can be extracted from processed tissue in high yield and with minimal or no degradation. Advantages of the solution include: economy and safety, easy access to archival material, and compatibility with both cellular and genetic analyses. The use and manufacture of the solution are also described.

Provided is an HPV E6, E7 mRNA assay, referenced herein as the “In Cell HPV Assay,” that is capable of sensitive and specific detection of normal cervical cells undergoing malignant transformation as well as abnormal cervical cells with pre-malignant or malignant lesions. The In Cell HPV Assay identifies HPV E6, E7 mRNA via in situ hybridization with oligonucleotides specific for HPV E6, E7 mRNA and quantitates the HPV E6, E7 mRNA via flow cytometry. The In Cell HPV Assay can be carried out in less than three hours directly from liquid-based cervical (“LBC”) cytology specimens. The In Cell HPV Assay provides an efficient and highly sensitive alternative to the Pap smear for determining abnormal cervical cytology.

Scanning and analysis of cytology and histology samples uses a flatbed scanner to capture images of the structures of interest such as tumor cells in a manner that results in sufficient image resolution to allow for the analysis of such common pathology staining techniques as ICC (immunocytochemistry), IHC (immunohistochemistry) or in situ hybridization. Very large volumes of such material are scanned in order to identify cells or clusters of cells which are positive or warrant more detailed examination, and if analysis at higher resolution is necessary, information regarding these positive events is transferred to a secondary microscope, such as a conventional scanning microscope, to allow further analysis and review of the selected regions of the slide containing the sample.

Raman molecular imaging is used to differentiate between normal and diseased cells or tissue. For instance benign and malignant lesions of bladder and other tissues can be distinguished, including epithelial tissues such as lung, prostate, kidney, breast, and colon, and non-epithelial tissues, such as bone marrow and brain. Raman scattering data relevant to the disease state of cells or tissue can be combined with visual image data to produce hybrid images which depict both a magnified view of the cellular structures and information relating to the disease state of the individual cells in the field of view.

This invention provides a novel device and method for preparing cytology slides. The device comprises a book-like form including an absorbent material and filter attached to the inside surface of a front cover and a cytology slide removeably attached to an inside surface of a back cover. A sample is removed from the body of a patient, placed in a liquid-based solution, and then on the filter. When the book-like form is closed, the sample is effectively transferred to the slide.

Raman molecular imaging is used to differentiate between normal and diseased cells or tissue. For instance benign and malignant lesions of bladder and other tissues can be distinguished, including epithelial tissues such as lung, prostate, kidney, breast, and colon, and non-epithelial tissues, such as bone marrow and brain. Raman scattering data relevant to the disease state of cells or tissue can be combined with visual image data to produce hybrid images which depict both a magnified view of the cellular structures and information relating to the disease state of the individual cells in the field of view.

The present invention provides a system and apparatuses / devices capable of automatically performing the processes of cell treatment preparatory to flow cytometry and similar cytological studies in a fully automated and streamlined manner. The system and apparatuses / devices are being adapted for preparation and (pre)processing of cell samples, e.g. blood and / or bone marrow samples.

A method and cytology device for manually adjusting the depth of endocervical sampling, and simultaneously sampling the endocervical canal and outer cervix using a dual-brush system. The device (38) comprises a primary cone-shaped endocervical sampling brush (40) adjacent to a secondary donut-shaped cervical sampling brush (42). The primary brush handle (52) is joined to the secondary brush handle (54) by means of a slidable rail (78), allowing adjustability of the primary brush length. The desired length and depth are visible by means of aligning incremental markings or measurements on the primary brush handle (80) with the positioning mark on the secondary brush handle (82). The brush handles are fixed in position and stabilized by means of a securing sheath (58), which has a pinch-release option to release or restore the friction existing between the sheath and the brush handles.

The invention discloses an exfoliative cells preserving fluid which is prepared from a pH buffering agent, an osmotic pressure maintenance agent, preservatives, a fixing agent for maintaining cellular morphology, an anticoagulant, a mucus softener, an antimicrobial reagent, a cleaning agent, a humectant and red blood cell destroying components. The components in the preserving fluid are reasonable in proportioning, and the exfoliative cells can be preserved at a long time under normal temperature, wherein the longest time can reach 2 years; mucus can be sufficiently dissolved and the red cells can be partially destroyed; a film production effect is good, cellular distribution is very even, cellular morphology is perfect, cytoplasm and cell nucleus demarcation is distinct, gradation is clear, and cytoplasm and cell nucleus transparency is very good, and the exfoliative cells preserving fluid can be simultaneously used for the HPV-DNA (human papillomavirus-deoxyribonucleic acid), Chlamydia and immunohistochemical test; the special liquid base preserving fluid used for the cytologic examination of other parts can be provided, an imported product can be completely replaced, and the cellular constituent with diagnostic significance can be sufficiently preserved; and the exfoliative cells preserving fluid is low in configuration cost and easy to popularize.

An apparatus for treating objects, in particular cytological or histological specimens, having multiple processing stations (2) and a transport device (4) for delivering the objects into and out of the processing stations (2), is characterized in that the transport device (4) comprises a robot arm (5) that is movable in three dimensions and provides for arbitrary positioning of the objects.

This invention provides a novel universal collection medium for cell collection. The medium allows for the first time the ability to perform cytology and direct molecular analysis on cells preserved in a single sample. This invention also provides novel methods for analyzing cells to assess human conditions.

Provided is an HPV E6, E7 mRNA assay, referenced herein as the “In Cell HPV Assay,” that is capable of sensitive and specific detection of normal cervical cells undergoing malignant transformation as well as abnormal cervical cells with pre-malignant or malignant lesions. The In Cell HPV Assay identifies HPV E6, E7 mRNA via in situ hybridization with oligonucleotides specific for HPV E6, E7 mRNA and quantitates the HPV E6, E7 mRNA via flow cytometry. The In Cell HPV Assay can be carried out in less than three hours directly from liquid-based cervical (“LBC”) cytology specimens. The In Cell HPV Assay provides an efficient and highly sensitive alternative to the Pap smear for determining abnormal cervical cytology.

Methods and devices for performing minimally invasive procedures useful for Fallopian tube diagnostics are disclosed. In at least one embodiment, the proximal os of the Fallopian tube is accessed via an intrauterine approach; an introducer catheter is advanced to cannulate and form a fluid tight seal with the proximal os of the Fallopian tube; a second catheter inside the introducer catheter is provided to track the length of the Fallopian tube and out into the abdominal cavity; a balloon at the end of the second catheter is inflated and the second catheter is retracted until the balloon seals the distal os of the Fallopian tube; irrigation is performed substantially over the length of the Fallopian tube; and the irrigation fluid is recovered for cytology or cell analysis.

The present invention provides a treatment method to excise a cancerous lesion, such as in the breast, with subsequent ablation of the margin. The method provides for location and excision with ablation under open guidance or guided imaging and for diagnosis by cytology. The method may be a minimally, invasive same day method with diagnosis before or immediately after excision. Also provided is a method of treating close or positive margins of an excisional site of a cancerous lesion in a breast by ablating the margin while monitoring the fluorescence of a fluorophor at the site to determine when ablation of the close or positive margin has occurred.

The invention belongs to the field of medical devices, and relates to a medical device for recovering targeted components of a cavity content. The device recovers the targeted components of the human body cavity content in vitro. The device can be used for greatly preparing a fecal bacterium solution with parasitic ova removed to support a preparation procedure of standardized fecal bacterium transplantation, used for greatly collecting the parasitic ova in a fecal suspension to increase a positive detection rate of the parasitic ova, used for collecting deslagged secretions in intestine and stomach cavities by an endoscope to meet requirements of checking and diagnosis, used for collecting lavage liquid in a trachea and removing slag by the endoscope to meet requirements of a hormone test and cytological examination, and used for collecting liquid of biliary and pancreatic ducts and a urogenital canal to meet requirements of the checking and the cytological examination. The method solves the practical problems of clinical applications, and has important innovation and an application value.

Provided herein are systems and methods that permit low resolution Raman spectroscopy to be used for detection of biological components within cells in order to classify the cells, for example, as premalignant, malignant, or benign.

The invention is directed toward a micro-endoscope assembly for the removal of tissue and cells from breast ducts comprising a cylindrical guide tube with a beveled distal end defining an internal cylindrical passageway and a first smaller cylindrical tube eccentrically formed in the cylindrical passageway of a smaller diameter than the tube internal cylindrical passageway and adapted to receive and guide an endoscope with a handle assembly wherein the smaller cylindrical tube together with an inner wall surface of the cylindrical guide tube forms a second passageway. A second conduit of a smaller diameter than the smaller cylindrical tube is mounted in the second passageway to divide the second passageway into two separate divided sections and is of sufficient diameter to receive a laser fiber and a micro-endoscope is mounted in the smaller cylindrical tube and a laser is mounted in the second cylindrical tube in the second passageway. The assembly is inserted into a mammary duct and the interior of the duct is viewed until an abnormality is determined in the duct. The tissue and cells from the abnormality area are dislodged, irrigated and aspirated through a suction channel to a removable collection device.

A device, system and method for cytology acquisition which may be performed with a swallowable in vivo device, specifically with a swallowable endoscopy capsule. The swallowable capsule may comprise a rotatable drum, a brush attached onto the drum for brushing against a tissue and acquiring cytology, and a porthole through which the brush may be in contact with the tissue.

The present invention provides a treatment method to excise a cancerous lesion, such as in the breast, with subsequent ablation of the margin. The method provides for location and excision with ablation under open guidance or guided imaging and for diagnosis by cytology. The method may be a minimally, invasive same day method with diagnosis before or immediately after excision. Also provided is a method of treating close or positive margins of an excisional site of a cancerous lesion in a breast by ablating the margin while monitoring the fluorescence of a fluorophor at the site to determine when ablation of the close or positive margin has occurred.

Disclosed is a method and apparatus for accomplishing a noninvasive screen for breast disease markers, including breast cancer markers and cytologically abnormal cells. Intraductal fluid is noninvasively aspirated using compression, heating and suction cycles. The removed sample is thereafter assayed for the presence of cytologically abnormal cells and / or one or more breast disease markers. Sample size and intraductal mobility of breast disease markers may be enhanced by retrograde introduction of a carrier. Devices and assays are also disclosed.

The present invention is directed to a fixative composition, the use of the fixative composition in preparing cytological or histological specimens, and a method of preparing particulate matter, such as cytology, hematology and microbiology specimens, for examination by collecting the particulate matter in a uniform layer, preferably a monolayer, and fixing the particles in a composition according to the present invention. The cytological, hematological, microbiological and histological fixative composition of the present invention contains an aldehyde cross-linker, a polyol and a detergent. The method of the present invention for preserving the particulate or histological specimen uses the fixative composition containing an aldehyde cross-linker, a polyol and a detergent.

A detection method includes preparing single cloning antibody by steps of antigen synthesis, mouse immune, cell fusion, hybrid tumor selection and its cell line collection, extracting ZEN in corn sample by methanol / aqueous solution; filtering and deluting it; flowing liquid sample through affinity column and it by methanol; and using fluoresclence spectrophotometer to measure out content of zearlenone in set sample solution.

The invention provides recombination interferon (rSIFN-co) or the functional analog with new space conformation, enhanced effect, and low side effect. It is showed by the external pharmacodynamics, it can not only restrain the DNA replication of the hepatitis b virus, but also restrain the exudation of the surface antigen (HBsAg) and e antigen (HBeAg). The cytology toxicity is 1 / 8 of the clinical interferon and the antiviral activity is 5-20 times to the clinical interferon and has a higher, a more board spectrum and longer time biological response reaction, and prevent the tumor hyperplasia and transmission. The invention also provides the synthetic gene code, gene carrier, expression system of the synthetic gene of the recombination interferon or the functional analog. Finally, the invention also provides the preparing method and application thereof.

The invention relates to a preparing method for a probe molecule which identifies tumor cells specifically, belonging to the nanometer material and biology technical field; the method comprises the following steps: (1) biocompatible folate is used for modifying PEG with the two sides being amino groups; (2) the PEG connected with the folate is modified to the surface of dendrimer PAMAM; (3) oil-soluble ligand on the surface of a quantum dot is replaced by a ligand exchange method, thus preparing a water-soluble quantum dot; the invention can lead the quantum dot to identify cancer cells expressed with high-level folate receptors, thus being capable of being used as a fluorescent probe used for the study of cytology; at the same time, the PAMAM and the PEG are modified to the surface of the quantum dot, thus improving the stability of the quantum dot greatly; furthermore, the PAMAM and the PEG have good biocompatibility and little toxicity, thus providing feasibility for the application thereof in the biology.

The invention provides the identification of oncogenic pathways activated in cytologically normal airway cells of individuals having or at risk of having lung disease, as well as specific gene expression patterns (biomarkers) associated with pathway activation. These biomarkers and pathways may provide prognostic and / or diagnostic indicators in lung disease, e.g., lung cancer. Additionally, these pathways and biomarkers may provide therapeutic targets for the treatment of lung disease, as well as markers for the assessment of treatment efficacy.

A filter assembly for separating and collecting a layer of particulate matter (e.g., cells) from a liquid containing the particulate matter (e.g., a biological fluid specimen). The filter assembly has a holder and contiguous inner and outer fluid pervious media. The outer fluid pervious medium is attached to the sidewall of the holder. The inner fluid pervious medium is of low fluidic impedance relative to the outer fluid pervious medium. The filter assembly is designed for use in a separation housing that defines two flow paths between inlet and outlet, one directly through the filter and the other around the periphery of the filter. The inlet portion of the housing has a central inlet in a radially sloped surface that faces the filter collection site. A movable suction head is adapted to cooperate with the housing and the filter assembly.