53 results about "Staining technique" patented technology

Scanning and analysis of cytology and histology samples uses a flatbed scanner to capture images of the structures of interest such as tumor cells in a manner that results in sufficient image resolution to allow for the analysis of such common pathology staining techniques as ICC (immunocytochemistry), IHC (immunohistochemistry) or in situ hybridization. Very large volumes of such material are scanned in order to identify cells or clusters of cells which are positive or warrant more detailed examination, and if analysis at higher resolution is necessary, information regarding these positive events is transferred to a secondary microscope, such as a conventional scanning microscope, to allow further analysis and review of the selected regions of the slide containing the sample.

The invention aims at providing a pathological diagnosis reagent, which is mainly used for processing various tissue samples in the pathology department in all levels of hospitals, can satisfy the requirements of a global non-xylene HE dyeing treating fluid of routine paraffin slices of biological tissue samples that is required in the slicing technique of the tissue samples with manual operation or operation of an automatic dehydrator and in the HE dyeing technique of cast-off cells, and is separated into two parts of tissue dehydration and section staining. As a novel conservation-conscious environmental protection technique, the pathological diagnosis reagent quickens splicing speed and simultaneously leads the tissue not to become over shrunk, hardened, split and crisp and leads the cells and the tissue structure to be closer to original patterns; the pathological diagnosis reagent has more complete tissue dehydration and adopts various novel mixed reagents that are pure analyzing organic chemical reagents with low volatility or non-volatility, non-toxicity and non-pollution, thus replacing originally widely used xylene reagents that have toxicity and heavy environmental pollution and eliminating occupational diseases that are caused by the xylene reagents in the global slicing of tissue sample preparation and tissue slice HE dyeing.

The present invention relates to a fluid waterless dyeing technique, in the concrete, it relates to a dyeing technique using carbon dioxide as dye carrier under the supercritical condition. The invented dyeing technique includes dyeing process and dyeing equipment. Its dyeing process includes the following steps: heating and pressurizing liquid CO2 in dyeing system and making said liquid CO2 into supercritical fluid; utilizing circulation pump to make CO2 be continuously and circularly reciprocated between dyeing tank and dye tank, dissolving dye while dyeing textile, then using new CO2 to wash dyed textile, after the dyed textile is washed, said equipment releases pressure, recovering CO2 and dye. Said dyeing equipment includes dyeing system and recovery separation system, said two systems one connected by means of pipeline.

The invention relates to an automatic bone marrow sample processing device, and an automatic analyzing and radiograph reading method thereof. The device combines automatic staining technology, sample quality automatic detection technology, encrpytion technology, microscopical technology and image processing technology, and can realize the all-around automation of staining, scanning and radiograph reading. The automatic radiograph reading method comprises the steps of firstly, performing low-power lens complete sample scanning and splicing, so as to obtain a low-power lens complete sample panoramic image, then inputting an interested characteristic parameter set, and finally performing high-power lens interested region scanning and splicing, so as to obtain a high-power lens interested region panoramic image set. Compared with the traditional bone marrow sample processing and radiograph reading method, the automatic analyzing and radiograph reading method has the advantages that the problem that the complete marrow sample quality cannot be checked by doctors on the whole is solved, and the radiograph reading speed and accuracy of doctors are improved. The low-power lens panoramic image and the high-power lens panoramic image set obtained can serve as an objective base and experimental data for clinical diagnosis and provide a necessary support for the development of myelopathy diagnostics, and are huge in social benefit.

The invention relates to a computing device-implemented method and apparatus for unsupervised segmentation of microscopic color image of unstained specimen and digital staining of segmented histological structures. Image of unstained specimen is created by light microscope 101, recorded by color camera 102 and stored on computer-readable medium 103. The invention is carried out by a computing device 104 comprised of: computer-readable medium for storing and computer for executing instructions of the algorithm for unsupervised segmentation of microscopic color image of unstained specimen and digital staining of segmented histological structures. Segmented histological structures and digitally stained image are stored and displayed on the output storing and display device 105 in order to establish diagnosis of a disease. The invention is an improvement over the prior art as it is characterized by the: (i) shortening of slide preparation process; (ii) reduction of intra-histologist variation in diagnosis; (iii) elimination of adding chemical effects on specimen; (iv) elimination of altering morphology of the specimen; (v) simplification of histological and intra-surgical tissue analysis; (vi) being significantly cheaper than existing staining techniques; (vii) being harmless to the user because toxic chemical stains are not used; (viii) discrimination of several types of histological structures present in the specimen; (ix) usage of the same specimen for more than one analysis.

The invention discloses a lens optical film product, at least comprising plastic layers and a color changing layer wherein the color changing layer is attached between the two plastic layers. One of the polarization layer, UV absorbing material layer or dye layer or the three arbitrary combination can be attached between the between the two plastic layers. The color changing layer is sandwiched between the two plastic layers using the attaching technique, rather than the color changing layer is coated on the plastic layers using staining technique, so as to avoid unevenly dyeing and prevent the color changing layer from falling off. Moreover the color changing layer is attached with the plastic layer after independently moulding, thus the color changing time can be well controlled to prevent the color changing time from being slow.

An “in-gel” staining technique for detecting and / or separating proteins during electrophoresis, illustratively as a modification of the standard Laemmli procedure. A fluorescent dye, such as Nile red or Phosphine, is included in the running buffer (mobile phase) which may be a standard Laemmli Tris-Glycine SDS buffer that has been modified to reduce the concentration of detergent (SDS) to less than the typical concentration (0.10% v / v). The fluorescent dye stains proteins during electrophoretic separation. The post-electrophoretic operations are, therefor, reduced and the separated, stained fractions are recoverable for further processing, purifying or analysis.

The invention belongs to the field of medical tests, and relates to a urinary cell micro staining analysis system, in particular to a urinary cell micro staining analysis method and an involved analyzing device. Combining with a computer automatic control and recognition technique, the invention provides a urinary cell micro staining quantitative system which can achieve cell separation, extraction and thin layer flaking of arena, support a single-flow micro staining technique, and aim at clinical large samples and rapid analysis. The system overcomes the defects of the prior art, and the results of urine sample clinical medical inspection practices show that the urinary cells are stained clearly, are distributed evenly and are easy to recognize and quantitate. The method can prevent sample cross contaminations and can perform precise quantitative analysis without staining solution environmental pollution. The method is in accordance with the requirements on arena examination standardization established by the inspection branches of International (NCCL) and Chinese Medical Association.

The invention discloses a method for researching the effects of Dickkopf-1 and cell apoptosis in steroid-induced avascular necrosis of femoral head (SANFH). The method comprises the following steps: by taking the femoral head sample of SANFH taken through total hip replacement as an experiment group and taking the sample after the hip replacement for fracture of neck of femur as a contrast group, cutting the femoral head sample, fixing and decalcifying the cut femoral head sample to prepare slices, observing the morphologic change of osteocytes by an electronic microscope, observing the pathological change of the osteocytes by a light microscope, counting the vacant bone lacuna, detecting the expression of Dickkopf-1 by an immunohistochemical staining technique, thus exploring the effects of Dickkopf-1 and cell apoptosis in SANFH. The method can be used for providing assistance for early diagnosis and curing of SANFH and also providing a theoretical basis for clinical prevention and curing of SANFH in future.

The invention belongs to the technical field of microbial fluorescent staining, and particularly relates to a genital tract secretion multi-fluorescent staining solution as well as a preparation method and application thereof. The genital tract secretion multi-fluorescent staining solution comprises fluorescein, nucleic acid dye, a bacteriostatic agent, an anti-quenching agent, a permeable agent and a buffer solution, and the pH is 4.5 to 6.0. The genital tract secretion multiple-fluorescence staining solution has short staining time on vaginal secretion samples, and is suitable for large-scale clinical work; the staining result is stable and reliable, the morphological structures of cells and various pathogenic microorganisms are clear, the contrast is distinct, and the morphology, size and distribution of various pathogenic microorganisms and the variety and number of various cells in the smear can be clearly observed under a microscope. In addition, the multi-fluorescent staining solution is good in stability and can be used for a long time, and the staining cost is greatly reduced.

The invention is applicable to the technical field of biological tissue imaging, and provides a tumor tissue imaging device and method. The tumor tissue imaging device comprises a light source, a polarizing part, a rotating wheel, a polarization analyzing part and a camera, wherein the light source, the polarizing part, the polarization analyzing part and the camera are distributed in a light pathdirection, and the rotating wheel is located between the polarizing part and the polarization analyzing part; and the rotating wheel is used for enabling the polarizing part or the polarization analyzing part to rotate according to a preset angle so as to switch imaging modes, and the imaging modes comprise reflection type imaging and transmission type imaging. Compared with an existing stainingtechnology, the requirement for pretreatment of tumor tissue to be detected is not high, the limitation on the size, the form and the light transmission of the tumor tissue to be measured is smaller,the measurement objects are wider, switching between the transmission type and reflection type can be freely realized, and the use flexibility and the applicability of the device are effectively improved.

According to the invention, a functional molecular marker Sly_miRNA164b of tomato fruit development associated genes is used to express tomato fruit development associated genes, and then GUS stainingtechnology can be used to rapidly verify development status of plant fruits. The method has simple and easy operations and obtains reliable results. Meanwhile, the correlation of the Sly_miRNA164b toshape and maturity of tomato fruits is obtained through functional molecular marker analysis. The functional molecular marker can be used to quickly, accurately and efficiently identify the shape andmaturity of the tomato fruits, provides a basis for the screening of tomatoes and offers important way for molecular markers in identification of germplasm resources.

Presented herein are methods of improving the consistency of staining with a counterstain. In some embodiments, the method makes use of an automated specimen processing apparatus or other staining device. In some embodiments, the staining methods are applied manually. In some embodiments, the counterstain includes eosin.

An object of the present invention is to provide a method for evaluating the physical strength of husks of a barley ingredient for malt manufacture. Barley kernels with husks are disposed in a sulfuric acid solution with a concentration of approximately 40% to 60%, and are agitated for a prescribed time (e.g. approximately 1 hour) using a stirrer bar or the like. After agitation, the barley kernels are treated with a mixed liquid of Methylene Blue and Eosin, and the degree of peeled husk (remaining degree) is examined by referring to the degree of dyed barley kernels, to thereby evaluate the physical strength of the husks of the barley kernels.

The present invention discloses a dyeing process of an anti-staining nylon color strip fabric cheese, and relates to the technical field of textile dyeing. The dyeing process comprises the following steps of (1) preparing a dye liquor; (2) performing dyeing; (3) preparing a color fixation liquid; (4) performing color fixation; and (5) carrying out cleaning. Dyeing of the nylon cheese is completedby virtue of combination of dyeing of an active dye and two times of color fixation, the dye-uptake reaches almost 100%, the nylon cheese after being dyed is high in color fastness, an anti-staining effect is achieved during processing of the color strip fabric, and especially the problem of staining after water washing of the color strip fabric can be avoided effectively, thereby ensuring the appearance quality of the color strip fabric.

The invention relates to the technical field of fluorescent staining, and particularly discloses a fluorescent staining solution and an application thereof. The fluorescent staining solution is prepared from the following raw materials: a fluorescent agent, a buffer solution, a preservative and water. The fluorescent staining solution disclosed by the invention can be used for staining epithelialcells, leukocytes, bacteria, fungi and parasites of human secretions or body fluids, and has the advantages of improving a microbiological detection rate and reducing a missed diagnosis rate and a misdiagnosis rate.

The invention provides a photochromic staining solution suitable for an anodic oxidation technology, and provides a preparation method and a staining technique of the staining solution. The photochromic staining solution comprises following components of, by weight, 10-30 parts of organic solvents, 0.5-1.5 parts of photochromic dye, 1-2 parts of light stabilizers, 1-2 parts of antioxidants, and 2-8 parts of polymer materials. During staining, the staining solution is subjected to ultrasonic dispersion for several minutes, a certain negative pressure state is maintained, thus presentation of the staining effect is facilitated, meanwhile, the hole sealing voltage is increased by 10%-50%, and the photochromic oxidation film layer surface with excellent chroma is also advantageously obtained.The staining solution and the technique are applied to various oxidation film layers containing porous structures, thus the evenly stained material surface with the obvious color changing effect, goodweather resistance, good abrasion resistance and good color fixation is obtained, and the special sandblasting or drawing texture of the material surface can further be reserved.

The invention provides a preparation method and application of heat transfer printing medium-temperature disperse dye ink, and relates to the technical field of dyeing. The heat transfer printing medium-temperature disperse dye ink is prepared from the following raw materials in percentage by mass: 0.5% to 9% of a medium-temperature disperse dye, 0.5% to 10% of a dispersing agent, 5% to 50% of a viscosity regulator, 0.01% to 5% of a surface tension regulator, 0.01% to 1% of an antibacterial agent and the balance of water. The disperse dye adopted by common heat transfer printing ink is the low-temperature disperse dye, the dye sublimation fastness is low, and heat transfer resistance is not achieved. In the transfer printing processing process, the thermal migration phenomenon occurs afterhigh-temperature treatment or in the long-term storage process. The heat transfer printing medium-temperature disperse dye ink is high in sublimation fastness and resistant to thermal migration, thethermal migration phenomenon cannot occur in the transfer printing processing process, and staining and color matching phenomena cannot occur in the storage and secondary processing process. The yieldand the customer satisfaction can be improved in the production process.

The invention relates to an acid-fast staining method and a quality monitoring method, and belongs to the technical field of tissue staining. The method comprises a formaldehyde detection step in the staining incubation process, when the formaldehyde concentration in the incubation environment is detected to be smaller than a threshold value, the staining process is judged to be qualified, and otherwise, the staining process is judged to be unqualified. By adopting the method, the quality of the acid-resistant staining process can be monitored, the problem of incapability of decoloring is avoided, and the detection accuracy is improved.

The invention discloses a method for detecting polysaccharide bacteria in sewage treatment activated sludge by using SYBR GREEN I single-staining technology, which belongs to the technical field of sewage biological treatment. According to the physical properties of polysaccharide bacteria combined with SYBR GREEN I single staining technology, the present invention detects its content in the sewage treatment system. The morphological characteristics of the whole bacteria were detected by flow cytometry, and the percentage of glycan bacteria in the whole bacteria was analyzed according to the size of the bacteria and the complexity of intracellular particles, and then the relative quantification of the glycan bacteria was done. The invention provides an economical, convenient and reliable quantitative method for polysaccharide bacteria in activated sludge in a sewage treatment system, and provides a theoretical basis for regulating and controlling biological phosphorus removal conditions in actual sewage treatment plants.

The invention discloses a phytophthora capsici cell division protein as well as an encoding gene and application thereof. The phytophthora capsici cell division protein can be a protein (a) which is formed by amino acid sequences 1 or a protein (b) which is formed by substituting amino acid sequences 1 by one or more amino acid residues and / or deleting amino acid sequences 1 and / or adding one or more amino acid residues, is related to plant salt resistance and is derived from amino acid sequences 1. Experiments prove that the phytophthora capsici cell division protein plays a role during the growth and development of phytophthora capsici, and the obtained silence transformants are obviously different from the wild phytophthora capsici in growth and development. The inoculation of hot pepper leaves against zoospores in vivo finds that the diseased spot areas of the inoculated silence transformants are obviously smaller than those of the wild phytophthora capsici. The research carried out on development of spores during staining by adopting the aniline blue staining technology proves that the spores of the silence transformants germinate late and the germinal tubes are short. The conclusions are helpful to the research on the development process and the molecular pathogenic mechanism of the phytophthora capsici to some extent.

The invention discloses a peanut chromosome probe staining kit and a method for using the same. The kit includes Probe Mix dry powder, DAPI mother solution with a concentration of 100 μg / ml, 2×SSC buffer, DAPI buffer, a dyeing cylinder and a variety control nucleus The probes in the Probe Mix dry powder include TAM fluorescently labeled DP-1, DP-7 and FAM fluorescently labeled DP-5, DP-8. The invention establishes a peanut probe staining technology and develops a probe staining kit, which is a new type of peanut banding analysis method. The method is simple, efficient and low-cost, and can be effectively used for peanut species identification and genome classification, etc., and lays the foundation for the next automated analysis and identification of peanut chromosomes.

The invention belongs to the field of medical tests, and relates to a urinary cell micro staining analysis system, in particular to a urinary cell micro staining analysis method and an involved analyzing device. Combining with a computer automatic control and recognition technique, the invention provides a urinary cell micro staining quantitative system which can achieve cell separation, extraction and thin layer flaking of arena, support a single-flow micro staining technique, and aim at clinical large samples and rapid analysis. The system overcomes the defects of the prior art, and the results of urine sample clinical medical inspection practices show that the urinary cells are stained clearly, are distributed evenly and are easy to recognize and quantitate. The method can prevent sample cross contaminations and can perform precise quantitative analysis without staining solution environmental pollution. The method is in accordance with the requirements on arena examination standardization established by the inspection branches of International (NCCL) and Chinese Medical Association.

The invention provides a method for rapidly identifying cadmium sensitivity of rice by using a fluorescent staining technology, and relates to the technical field of rice breeding. The method for rapidly identifying the cadmium sensitivity of the rice by using the fluorescent staining technology comprises the following steps: S1, stress treatment; and S2, microscope observation. According to method, cadmium sensitivities of different genotypes of rice are intuitively and accurately judged by utilizing correlation between quantity of active oxygen generated by in-vitro leaves in cadmium stress environment and fluorescence intensity, and cadmium sensitivities of different genotypes are identified on basis of fluorescence intensity and distribution characteristics in rice leaves. According to the method, only a small number of rice leaves are needed, the method is not limited by the size of the rice, and the cadmium tolerance of the rice can be rapidly and accurately identified in a short time. The method has the advantages of being high in efficiency, good in repeatability, low in cost and the like, a large number of genotypes sensitive to cadmium tolerance are screened according to the leaf fluorescence intensity identification technology, the scale of field trials can be effectively reduced, and the method is worthy of being vigorously popularized.

The invention belongs to the technical field of cell slide dyeing, and mainly relates to an automatic slide dyeing system which comprises a slide conveying mechanism, a dyeing liquid storage bin, a dyeing liquid dropwise adding mechanism, a controller, a dyeing bin, a uniform mixing mechanism, a flushing mechanism, a drying mechanism and a waste liquid collecting system. The slide conveying mechanism is used for conveying dyed slides and taking the dyed slides out of the dyeing area; the staining solution dropwise adding mechanism is used for dropwise adding different staining solutions to a to-be-stained area of the slide according to a preset program; the uniform mixing mechanism is used for stirring the staining fluid on the slide to ensure uniform staining; the flushing mechanism is used for cleaning redundant staining fluid and impurities on the stained slide; the drying mechanism is used for drying the cleaned slide; the controller is used for storing a preset dyeing scheme and controlling all the mechanisms to complete automatic dyeing according to the dyeing scheme. The system can be used for stably and quickly dyeing the slide.

The invention discloses a composite super-living body S staining solution, and relates to the technical field of urine detection staining, in particular to the composite super-living body S staining solution which comprises the following raw materials: 1-20mmol / L of alcian blue; 8 to 25 mmol / L of acid blue; 1 to 20 mmol / L of piperidine B; 10 to 35 mmol / L of sand yellow; the concentration of the MES Buffer is 0.1 mol / L. The composite super-living body S staining solution can only stain urine visible components while inhibiting staining of impurities in urine, and can reduce background interference and effectively inhibit mixing of impurities during urinary sediment detection by inhibiting staining of urine sediments during urinary sediment detection, so that a method capable of effectively improving the detection rate of target cells is provided, and the detection efficiency of the target cells is improved. Furthermore, when urine sediment is detected and analyzed, it is possible to sensitively detect rare components such as renal tubular epithelial cells and casts, and to perform a highly sensitive urinary sediment test by increasing the rate of detection of rare components such as epithelial cells and casts in a urine sample. The present invention enables highly accurate analysis of visible components in a urine sample using an automatic analyzer.

The invention provides a silk fabric staining method by using gardenin, the silk fabric staining method comprises the following steps: dissolving the gardenin into a carbon tetrachloride / phenol mixed solution, then adding a surfactant sodium dodecyl benzene sulfonate, obtaining a transparent solution under ultrasonic; putting a silk fabric into the transparent solution, oscillating and heating up to 30 to 60 DEG C to be stained. The staining technique provided by the invention has the advantages that modification on the gardenin is not needed, high temperature staining is not adopted, and the stained fabric is high in color fastness.

The invention discloses a method for flow cytometry analysis of stem cell cytokines, and provides a staining technology capable of truly simulating in-vivo conditions of stress hematopoiesis of hematopoietic stem cells through proper culture and stimulation conditions and accurately detecting the expression condition of the stem cell cytokines in the hematopoietic stem cells through a fixed membrane rupture flow cytometry analysis technique. The method is simple and convenient to operate and low in requirements on cell suspension preparation operation, and the stress hematopoietic stem cells conforming to the real condition of in-vivo infection can be obtained only by skillfully mastering a cell culture technology and conducting culturing according to the conditions designed by the technology. Moreover, operations such as chip preparation are not needed, so cost is relatively low. Extracellular cytokines secreted by a compound are retained in cells, and intracellular and extracellular cytokine expression conditions of the cells can be effectively and accurately measured. A technical foundation is laid for further deeply discussing a regulation mechanism of cytokine expression and secretion of hematopoietic stem precursor cells.

The invention relates to a pathological section staining technology, in particular to a flushing device for staining in the pathology department. The flushing device comprises a tank body; the tank body is used for containing water, and a partition plate slidably penetrates through the tank body and divides the tank body into an upper cavity and a lower cavity; a water outlet is formed in the peripheral wall of the tank body; the upper end of a telescopic rod of the telescopic cylinder is fixedly connected with the lower end of the partition plate, and the lower end of the telescopic cylinder is fixedly connected with the lower bottom wall of the tank body; the telescopic cylinder is electrically connected with a controller; a tank cover comprises a cover body, the cover body is used for covering the upper end opening of the tank body, a cavity between the cover body and the partition plate is used for containing water, a through via hole is formed in the upper end of the cover body, a threaded rod movably penetrates through the via hole in a sleeved mode and is in threaded connection with a nut, and a liquid level sensor is fixed to the lower end of the threaded rod; the liquid level sensor is electrically connected with the controller; and a water valve is arranged on a hose; one end of a pipeline is communicated with one end of a water outlet through the hose, and the lower side face of the pipeline is fixedly connected with a supporting plate. With the flushing device adopted,water flow speed is constant, a slice tissue is not washed away, and the water temperature is controllable.